Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,016,166	A→G	Y36C (TAC→TGC)	ygfK →	putative Fe‑S subunit oxidoreductase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,016,166	0	A	G	100.0%	205.2 / NA	54	Y36C (TAC→TGC)	ygfK	putative Fe‑S subunit oxidoreductase subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/26);  total (28/26)

CCGGTCACTCAATGATATCTGTATAAGCTAAGGAGAGGGTTATGGGGGATATTATGCGTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTAAAAACC  >  NC_000913/3016019‑3016311                                                                                                                                                    |                                                                                                                                                  ccGGTCACTCAATGATATCTGTATAAGCTAAGGAGAGGGTTATGGGGGATATTATGCGTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTc                                                                                                                                                <  2:455465/151‑1 (MQ=255)  cGGTCACTCAATGATATCTGTATAAGCTAAGGAGAGGGTTATGGGGGATATTATGCGTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTc                                                                                                                                                >  2:837798/1‑150 (MQ=255)   ggTCACTCAATGATATCTGTATAAGCTAAGGAGAGGGTTATGGGGGATATTATGCGTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCAc                                                                                                                                              >  2:121892/1‑151 (MQ=255)        cTCAATGATATCTGTATAAGCTAAGGAGAGGGTTATGGGGGATATTATGCGTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGt                                                                                                                                          >  2:740939/1‑150 (MQ=255)           aaTGATATCTGTATAAGCTAAGGAGAGGGTTATGGGGGATATTATGCGTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTaaaa                                                                                                                                      <  1:99121/151‑1 (MQ=255)                     gTATAAGCTAAGGAGAGGGTTATGGGGGATATTATGCGTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACt                                                                                                                             <  2:137557/150‑1 (MQ=255)                     gTATAAGCTAAGGAGAGGGTTATGGGGGATATTATGCGTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTg                                                                                                                            <  1:365321/151‑1 (MQ=255)                       ataAGCTAAGGAGAGGGTTATGGGGGATATTATGCGTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGtt                                                                                                                          <  1:149739/151‑1 (MQ=255)                         aaGCTAAGGAGAGGGTTATGGGGGATATTATGCGTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTa                                                                                                                         <  1:510099/150‑1 (MQ=255)                             tAAGGAGAGGGTTATGGGGGATATTATGCGTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTc                                                                                                                    >  2:448183/1‑151 (MQ=255)                              aaGGAGAGGGTTATGGGGGATATTATGCGTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCt                                                                                                                   >  1:908240/1‑151 (MQ=255)                                ggAGAGGGTTATGGGGGATATTATGCGTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACTAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTc                                                                                                                 >  1:62619/1‑151 (MQ=255)                                 gagaGGGTTATGGGGGATATTATGCGTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCg                                                                                                                <  1:685057/151‑1 (MQ=255)                                    agGGATATGGGGGATATTATGCGTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGt                                                                                                              >  2:652132/1‑150 (MQ=255)                                       gTTATGGGGGATATTATGCGTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGaaa                                                                                                          <  2:908167/151‑1 (MQ=255)                                       gTTATGGGGGATATTATGCGTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGaa                                                                                                           >  1:440932/1‑150 (MQ=255)                                         tATGGGGGATATTATGCGTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAGCAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAAcc                                                                                                        >  1:534588/1‑151 (MQ=255)                                          aTGGGGGATATTATGCGTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCt                                                                                                       >  2:385440/1‑151 (MQ=255)                                          aTGGGGGATATTATGCGTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCt                                                                                                       >  1:844741/1‑151 (MQ=255)                                           tGGGGGATATTATGCGTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCtg                                                                                                      >  2:70899/1‑151 (MQ=255)                                                gATATTATGCGTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGcc                                                                                                  >  1:310910/1‑150 (MQ=255)                                                   attatGCGTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTc                                                                                              <  2:820022/151‑1 (MQ=255)                                                   attatGCGTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTc                                                                                              >  2:7763/1‑151 (MQ=255)                                                      atGCGTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCg                                                                                           <  2:531350/151‑1 (MQ=255)                                                       tGCGTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTccca                                                                                           <  2:742450/150‑2 (MQ=255)                                                         cGTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCg                                                                                        <  1:302803/151‑1 (MQ=255)                                                         cGTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCg                                                                                        <  2:226371/151‑1 (MQ=255)                                                               aTTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTg                                                                                  >  2:189525/1‑151 (MQ=255)                                                                         aGGAAATTGTGACGCGCATATTTGATGAATACCAACAACAAAGCTCAATCTTTGATATTCCCGAGAAACAGGTTTGATCACCTGTAAAAGGGAAAACTGTTAGCGTCTTGGGAGAAACCTGTGCCACTCCCGTCGGCTCTGCCGCTGGccc                                                                        >  2:229934/1‑151 (MQ=255)                                                                            aaCTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGc                                                                      >  1:131295/1‑150 (MQ=255)                                                                                   gACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGc                                                                >  1:893790/1‑149 (MQ=255)                                                                                     cgcgCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCt                                                            <  1:642767/151‑1 (MQ=255)                                                                                          atatTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCgcgc                                                       >  2:819862/1‑151 (MQ=255)                                                                                            atTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCa                                                      >  1:109757/1‑150 (MQ=255)                                                                                            atTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCa                                                      <  1:599708/150‑1 (MQ=255)                                                                                                    aaTACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTg                                              <  2:719611/150‑1 (MQ=255)                                                                                                     aTACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCTTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTg                                              <  1:421096/149‑1 (MQ=255)                                                                                                         caacaacaaCGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACtt                                        >  2:587139/1‑151 (MQ=255)                                                                                                              acaacGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTgg                                   >  2:80168/1‑151 (MQ=255)                                                                                                               caacGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGc                                  <  2:560613/151‑1 (MQ=255)                                                                                                                      cAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTgg                           >  1:861016/1‑151 (MQ=255)                                                                                                                       aaTCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGc                          <  1:424821/151‑1 (MQ=255)                                                                                                                            ttGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGa                       <  1:749091/149‑1 (MQ=255)                                                                                                                                tATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCCCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTc                 >  1:111186/1‑151 (MQ=255)                                                                                                                                 aTTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTc                 <  2:146029/150‑1 (MQ=255)                                                                                                                                 aTTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCa                <  1:448230/151‑1 (MQ=255)                                                                                                                                    cccGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCg             <  1:591555/151‑1 (MQ=255)                                                                                                                                        aGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACt         >  1:566452/1‑151 (MQ=255)                                                                                                                                          cAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTaa       <  2:566521/151‑1 (MQ=255)                                                                                                                                          cAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTa        >  1:567957/1‑150 (MQ=255)                                                                                                                                           aaCAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTaa       >  1:729300/1‑150 (MQ=255)                                                                                                                                              aGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTAAAAAc   >  1:159922/1‑151 (MQ=255)                                                                                                                                              aGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTAAAAAc   <  2:398958/151‑1 (MQ=255)                                                                                                                                               gTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTAAAAAcc  <  1:478105/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                  CCGGTCACTCAATGATATCTGTATAAGCTAAGGAGAGGGTTATGGGGGATATTATGCGTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTAAAAACC  >  NC_000913/3016019‑3016311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑