Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,083,334	T→C	Y155C (TAT→TGT)	speB ←	agmatinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,083,334	0	T	C	100.0%	195.9 / NA	56	Y155C (TAT→TGT)	speB	agmatinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/22);  total (34/22)

ACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGTGCG  >  NC_000913/3083187‑3083478                                                                                                                                                    |                                                                                                                                                 aCCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTCCGCACAgg                                                                                                                                               >  1:824184/1‑151 (MQ=255)   cTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGt                                                                                                                                              >  1:583993/1‑150 (MQ=255)   cTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGt                                                                                                                                              >  1:333616/1‑150 (MQ=255)              ccAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGGACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGc                                                                                                                                  >  1:205774/1‑151 (MQ=255)                   aCGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCa                                                                                                                              >  1:96698/1‑150 (MQ=255)                    cGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCaa                                                                                                                             >  2:481301/1‑150 (MQ=255)                     ggTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAg                                                                                                                           <  1:205062/151‑1 (MQ=255)                      gTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAgt                                                                                                                          >  1:593983/1‑151 (MQ=255)                        aaaGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAgtgt                                                                                                                        >  1:47660/1‑151 (MQ=255)                          aGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTAc                                                                                                                      <  1:438783/151‑1 (MQ=255)                           gCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTAcc                                                                                                                     >  1:634943/1‑151 (MQ=255)                           gCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTAcc                                                                                                                     <  2:10816/151‑1 (MQ=255)                            ccGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTAcc                                                                                                                     >  2:209571/1‑150 (MQ=255)                                   cTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCAt                                                                                                             >  1:340392/1‑151 (MQ=255)                                       aTCAAACGCGGTACGAATACCAATCTGCACTGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAAGCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCAtttt                                                                                                          <  2:568024/150‑1 (MQ=255)                                          aaaCTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCg                                                                                                      >  2:664393/1‑151 (MQ=255)                                            aCTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGa                                                                                                     >  1:579861/1‑150 (MQ=255)                                               cGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATg                                                                                                 <  1:550218/151‑1 (MQ=255)                                                 gTACGAATCCCAATCTGCACGGAAGGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGGCAAATTCACAACCGTTCGCACAGGTATCGGTGGGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAAt                                                                                                  >  2:279937/1‑148 (MQ=255)                                                 gTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCt                                                                                               >  2:718881/1‑151 (MQ=255)                                                 gTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCt                                                                                               <  2:529722/151‑1 (MQ=255)                                                    cGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTc                                                                                             <  1:209338/150‑1 (MQ=255)                                                     gAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGc                                                                                           >  1:263473/1‑151 (MQ=255)                                                     gAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGc                                                                                           >  1:104599/1‑151 (MQ=255)                                                      aaTACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCa                                                                                          <  2:283552/151‑1 (MQ=255)                                                         aCCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATg                                                                                        <  1:84679/150‑1 (MQ=255)                                                            aaTCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGc                                                                                     >  2:368242/1‑150 (MQ=255)                                                            aaTCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGc                                                                                     >  2:772873/1‑150 (MQ=255)                                                            aaTCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGAGCCATTTTGCCGAAATGCTTCGCATGAGc                                                                                     >  2:194830/1‑150 (MQ=255)                                                                  cACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACgcag                                                                               >  1:315405/1‑150 (MQ=255)                                                                     ggAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCagcagc                                                                           >  2:680674/1‑151 (MQ=255)                                                                        aTGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAgcagcggc                                                                        <  2:682575/151‑1 (MQ=255)                                                                          gATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCCCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAgcagcggcag                                                                      <  2:237420/151‑1 (MQ=255)                                                                           aTTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAgcagcggcagc                                                                     >  2:45986/1‑151 (MQ=255)                                                                             tCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAgcagcggcagcg                                                                    <  1:836486/150‑1 (MQ=255)                                                                             tCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAgcagcggcagcg                                                                    <  1:289144/150‑1 (MQ=255)                                                                               gggTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTa                                                                  >  1:400437/1‑150 (MQ=255)                                                                                     aTCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGt                                                           >  1:330369/1‑151 (MQ=255)                                                                                      tCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTg                                                          >  2:719745/1‑151 (MQ=255)                                                                                         gACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTc                                                       >  1:107592/1‑151 (MQ=255)                                                                                           ccTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTcac                                                     >  1:149811/1‑151 (MQ=255)                                                                                              tcttTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTcacca                                                   <  2:877403/150‑1 (MQ=255)                                                                                                  tCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGa                                               >  1:709385/1‑150 (MQ=255)                                                                                                       gcgGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAagaga                                         <  1:117161/151‑1 (MQ=255)                                                                                                             taGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCAt                                     <  2:824117/149‑1 (MQ=255)                                                                                                                 aCATAGTGCCGTGGTCAAATTCACAACCGTTCGCCCAGGTATCCGTGTGGGCGTCAAAGTGTACCAGCGCCCTTTTGCCTAAATGCTCCCCATTAGCACGCAGCAGCTGCAGCATAACAAAGTGGTCACCCCCGACAGAGAGCATTCGCt                                <  2:701704/150‑1 (MQ=255)                                                                                                                 aCATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCtt                               >  1:596651/1‑151 (MQ=255)                                                                                                                    tAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTAcc                            >  1:638060/1‑151 (MQ=255)                                                                                                                      gTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCgg                          <  1:80243/151‑1 (MQ=255)                                                                                                                        gCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGc                         >  1:125784/1‑150 (MQ=255)                                                                                                                             ggTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGcagccag                   <  1:609796/151‑1 (MQ=255)                                                                                                                                 aaaTTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCagcagc               >  2:827931/1‑151 (MQ=255)                                                                                                                                      cacaACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTtctc          >  1:467487/1‑151 (MQ=255)                                                                                                                                          aCCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCg      <  1:765303/151‑1 (MQ=255)                                                                                                                                               tCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGTgcg  <  1:664442/150‑1 (MQ=255)                                                                                                                                               tCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGTgcg  <  1:191806/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                 ACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGTGCG  >  NC_000913/3083187‑3083478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑