Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,113,403 A→G C25C (TGT→TGC)  yghG ← secretin (GspDbeta) OM localization lipoprotein pilotin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,113,4030AG100.0% 227.8 / NA 68C25C (TGT→TGC) yghGsecretin (GspDbeta) OM localization lipoprotein pilotin
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/42);  total (26/42)

TCGCTGATAATGGTCATTTTTACCGTTGTGCCGCTACTTTGCGCCAGCACTAAGGTATATCCCGCAGATTTAATCGGCAGGTTTTGGCTGATATTTTGCGCCTG‑TTTTTTCGCCAGTAAACTGGCATTTTCATTATGGCTGGCACAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATACCCTCCCTGGCATTTGTTTTATCGACATGGTAAATAATCTCTAAGGTTATTAATAAGAGTTAAAATGTCACTTTGATAATGACGTGGTTATCATTA  >  NC_000913/3113260‑3113545
                                                                                                                                                |                                                                                                                                              
tCGCTGATAATGGTCATTTTTACCGTTGTGCCGCTACTTTGCGCCAGCACTAAGGTATATCCCGCAGATTTAATCGGCAGGTTTTGGCTGATATTTTGCGCCTG‑TTTTTTCGCCAGTAAACTGGCATTTTCATTATGGCTGGCGCAGcc                                                                                                                                           <  2:74854/149‑1 (MQ=255)
tCGCTGATAATGGTCATTTTTACCGTTGTGCCGCTACTTTGCGCCAGCACTAAGGTATATCCCGCAGATTTAATCGGCAGGTTTTGGCTGATATTTTGCGCCTG‑TTTTTTCGCCAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCa                                                                                                                                          >  1:677666/1‑150 (MQ=255)
         aTGGTCATTTTTACCGTTGTGCCGCTACTTTGCGCCAGCACTAAGGTATATCCCGCAGATTTAATCGGCAGGTTTTGGCTGATATTTTGCGCCTG‑TTTTTTCGCCAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATc                                                                                                                                <  2:187776/151‑1 (MQ=255)
           ggTCATTTTTACCGTTGTGCCGCTACTTTGCGCCAGCACTAAGGTATATCCCGCAGATTTAATCGGCAGGTTTTGGCTGATATTTTGCGCCTG‑TTTTTTCGCCAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCt                                                                                                                              <  1:213981/151‑1 (MQ=255)
              cATTTTTACCGTTGTGCCGCTACTTTGCGCCAGCACTAAGGTATATCCCGCAGATTTAATCGGCAGGTTTTGGCTGATATTTTGCGCCTG‑TTTTTTCGCCAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGt                                                                                                                            <  1:155619/150‑1 (MQ=255)
                 ttttACCGTTGTGCCGCTACTTTGCGCCAGCACTAAGGTATATCCCGCAGATTTAATCGGCAGGTTTTGGCTGATATTTTGCGCCTG‑TTTTTTCGCCAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAAc                                                                                                                         >  1:130747/1‑150 (MQ=255)
                       cGTTGTGCCGCTACTTTGCGCCAGCACTAAGGTATATCCCGCAGATTTAATCGGCAGGTTTTGGCTGATATTTTGCGCCTG‑TTTTTTCGCCAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTcaac                                                                                                                  >  2:398925/1‑151 (MQ=255)
                           gtgCCGCTACTTTGCGCCAGCACTAAGGTATATCCCGCAGATTTAATCGGCAGGTTTTGGCTGATATTTTGCGCCTGTTTTTTTCGCCAGTAAACTGGCTTTTTCATTATGGCTGGCGCAGCCCTTTAATAATCCTTTAACGCTaaatcgg                                                                                                               >  2:401066/1‑147 (MQ=255)
                             gCCGCTACTTTGCGCCAGCACTAAGGTATATCCCGCAGATTTAATCGGCAGGTTTTGGCTGATATTTTGCGCCTG‑TTTTTTCGCCAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGc                                                                                                            <  2:550544/151‑1 (MQ=255)
                              ccGCTACTTTGCGCCAGCACTAAGGTATATCCCGCAGATTTAATCGGCAGGTTTTGGCTGATATTTTGCGCCTG‑TTTTTTCGCCAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCg                                                                                                           <  2:649496/151‑1 (MQ=255)
                               cGCTTCTTTGCGCCAGCACTAAGGTATATCCCGCAGATTTAATCGGCAGGTTTTGGCTGATATTTTGCGCCTGTTTTTTTCGCCAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCt                                                                                                                      <  1:401092/140‑1 (MQ=255)
                                gCTACTTTGCGCCAGCACTAAGGTATATCCCGCAGATTTAATCGGCAGGTTTTGGCTGATATTTTGCGCCTG‑TTTTTTCGCCAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCg                                                                                                           <  1:398914/149‑1 (MQ=255)
                                       tGCGCCAGCACTAAGGTATATCCCGCAGATTTAATCGGCAGGTTTTGGCTGATATTTTGCGCCTG‑TTTTTTCGCCAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTaa                                                                                                    >  2:261607/1‑149 (MQ=255)
                                        gcgcCAGCACTAAGGTATATCCCGCAGATTTAATCGGCAGGTTTTGGCTGATATTTTGCGCCTG‑TTTTTTCGCCAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATAc                                                                                                 <  1:527759/151‑1 (MQ=255)
                                         cgcCAGCACTAAGGTATATCCCGCAGATTTAATCGGCAGGTTTTGGCTGATATTTTGCGCCTG‑TTTTTTCGCCAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATAcc                                                                                                <  2:103071/151‑1 (MQ=255)
                                          gcCAGCACTAAGGTATATCCCGCAGATTTAATCGGCAGGTTTTGGCTGATATTTTGCGCCTG‑TTTTTTCGCCAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTaa                                                                                                    >  2:626290/1‑146 (MQ=255)
                                           ccAGCACTAAGGTATATCCCGCAGATTTAATCGGCAGGTTTTGGCTGATATTTTGCGCCTG‑TTTTTTCGCCAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATAccct                                                                                              <  1:564017/151‑1 (MQ=255)
                                             aGCACTAAGGTATATCCCGCAGATTTAATCGGCAGGTTTTGGCTGATATTTTGCGCCTG‑TTTTTTCGCCAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATAccctcc                                                                                            >  1:737054/1‑151 (MQ=255)
                                               cACTAAGGTATATCCCGCAGATTTAATCGGCAGGTTTTGGCTGATATTTTGCGCCTG‑TTTTTTCGCCAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATAccctccct                                                                                          <  1:790292/151‑1 (MQ=255)
                                               cACTAAGGTATATCCCGCAGATTTAATCGGCAGGTTTTGGATGATATTTTGCGCCTG‑TTTTTTCGCCAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATAccctccc                                                                                           >  2:654297/1‑150 (MQ=255)
                                                 cTAAGGTATATCCCGCAGATTTAATCGGCAGGTTTTGGCTGATATTTTGCGCCTG‑TTTTTTCGCCAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATACCCTCCCTgg                                                                                        >  1:562042/1‑151 (MQ=255)
                                                        atatCCCGCAGATTTAATCGGCAGGTTTTGGCTGATATTTTGCGCCTG‑TTTTTTCGCCAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATACCCTCCCTGGCATTTg                                                                                  <  2:189358/150‑1 (MQ=255)
                                                        atatCCCGCAGATTTAATCGGCAGGTTTTGGCTGATATTTTGCGCCTG‑TTTTTTCGCCAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATACCCTCCCTGGCATTTGt                                                                                 <  1:436499/151‑1 (MQ=255)
                                                          atCCCGCAGATTTAATCGGCAGGTTTTGGCTGATATTTTGCGCCTG‑TTTTTTCGCCAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATACCCTCCCTGGCATTTGtt                                                                                <  1:465145/150‑1 (MQ=255)
                                                                cAGATTTAATCGGCAGGTTTTGGCTGATATTTTGCGCCTG‑TTTTTTCGCCAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATACCCTCCCTGGCATTTGTTTTATCGa                                                                         <  2:778294/151‑1 (MQ=255)
                                                                 aGATTTAATCGGCAGGTTTTGGCTGATATTTTGCGCCTG‑TTTTTTCGCCAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATACCCTCCCTGGCATTTGTTTTATCGAc                                                                        >  1:218042/1‑151 (MQ=255)
                                                                   aTTTAATCGGCAGGTTTTGGCTGATATTTTGCGCCTG‑TTTTTTCGCCAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATACCCTCCCTGGCATTTGTTTTATCGACAt                                                                      <  2:740579/151‑1 (MQ=255)
                                                                            gCAGGTTTTGGCTGATATTTTGCGCCTG‑TTTTTTCGCCAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATACCCTCCCTGGCATTTGTTTTATCGACATGGTAaata                                                              >  1:333093/1‑150 (MQ=255)
                                                                             cAGGTTTTGGCTGATATTTTGCGCCTG‑TTTTTTCGCCAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATACCCTCCCTGGCATTTGTTTTATCGACATGGTAaataat                                                            <  2:728435/151‑1 (MQ=255)
                                                                               ggTTTTTGCTGATATTTTGCGCCTG‑TTTTTTCGCCAGGAAGCTTGCATTTTCATTATGGCTGGCGCAGCCACTTATTAATCCTGTGATGCTCAACATTAGCGCTATTATAACCCTCCCGGGCATTTGTTTTATCGATATGGTaaa                                                                <  2:400392/145‑1 (MQ=255)
                                                                                gTTTTGGCTGATATTTTGCGCCTG‑TTTTTTCGCCAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATACCCTCCCTGGCATTTGTTTTATCGACATGGTAAATAAtctc                                                         <  2:128023/151‑1 (MQ=255)
                                                                                 ttttGGCTAATATGTTGCGCCGG‑TTTTTTCGCCAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATAGTCTCCCTGGCATTTGTTTTATCGACATGGTAAATAAtctc                                                         <  2:72326/150‑1 (MQ=255)
                                                                                  tttGGCTGATATTTTGCGCCTG‑TTTTTTCGCCAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATACCCTCCCTGGCATTTGTTTTATCGACATGGTAAATAATctct                                                        <  2:773780/150‑1 (MQ=255)
                                                                                     ggCTGATATTTTGCGCCTG‑TTTTTTCGCCAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATACCCTCCCTGGCATTTGTTTTATCGACATGGTAAATAATCTCTAAgg                                                    >  2:623522/1‑151 (MQ=255)
                                                                                         gATATTTTGCGCCTG‑TTTTTTCGCCAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATACCCTCCCTGGCATTTGTTTTATCGACATGGTAAATAATCTCTAAGGttat                                                <  2:228500/151‑1 (MQ=255)
                                                                                         gATATTTTGCGCCTG‑TTTTTTCGCCAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATACCCTCCCTGGCATTTGTTTTATCGACATGGTAAATAATCTCTAAGGtt                                                  <  2:426309/149‑1 (MQ=255)
                                                                                          atatTTTGCGCCTG‑TTTTTTCGCCAGTAAACTGGCATTTTCATTATGGCTGGCGCAACCACTTAATAATCCTGTAAAGCTCAACAATAGCGATATTAATACCCTCCCTGGCATTTGTTTTATCGACATGGTAAATAATCTCTAAGGttatt                                               >  1:339310/1‑151 (MQ=255)
                                                                                           tatTTTGCGCCTG‑TTTTTTCGCCAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATACCCTCCCTGGCATTTGTTTTATCGACATGGTAAATAATCTCTAAGGttatt                                               <  1:626339/150‑1 (MQ=255)
                                                                                            atTTTGCGCCTG‑TTTTTTCGCCAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATACCCTCCCTGGCATTTGTTTTATCGACATGGTAAATAATCTCTAAGGTTATtaa                                             <  1:903713/151‑1 (MQ=255)
                                                                                               ttGCGCCTG‑TTTTTTCGCCAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATACCCTCCCTGGCATTTGTTTTATCGACATGGTAAATAATCTCTAAGGTTATtaata                                           <  2:281654/150‑1 (MQ=255)
                                                                                               ttGCGCCTG‑TTTTTTCGCCAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATACCCTCCCTGGCATTTGTTTTATCGACATGGTAAATAATCTCTAAGGTTATtaata                                           >  2:110213/1‑150 (MQ=255)
                                                                                                 gcgcCTG‑TTTTTTCGCCAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATACCCTCCCTGGCATTTGTTTTATCGACATGGTAAATAATCTCTAAGGTTATTAATAaga                                        <  1:810353/151‑1 (MQ=255)
                                                                                                 gcgcCTG‑TTTTTTCGCCAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATACCCTCCCTGGCATTTGTTTTATCGACATGGTAAATAATCTCTAAGGTTATTAATAag                                         <  1:445880/150‑1 (MQ=255)
                                                                                                  cgcCTG‑TTTTTTCGCCAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATACCCTCCCTGGCATTTGTTTTATCGACATGGTAAATAATCTCTAAGGTTATTAATAagag                                       <  1:396898/151‑1 (MQ=255)
                                                                                                    ccTG‑TTTTTTCGCCAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATACCCTCCCTGGCATTTGTTTTATCGACATGGTAAATAATCTCTAAGGTTATTAATAAGAGtt                                     <  1:40180/151‑1 (MQ=255)
                                                                                                     cTG‑TTTTTTCGCCAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATACCCTCCCTGGCATTTGTTTTATCGACATGGTAAATAATCTCTAAGGTTATTAATAAGAGtt                                     >  1:517082/1‑150 (MQ=255)
                                                                                                           ttttCGCCAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATACCCTCCCTGGCATTTGTTTTATCGACATGGTAAATAATCTCTAAGGTTATTAATAAGAGTTAAAAt                                <  2:794998/150‑1 (MQ=255)
                                                                                                           ttttCGCCAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATACCCTCCCTGGCATTTGTTTTATCGACATGGTAAATAATCTCTAAGGTTATTAATAAGAGTTAAAAt                                <  2:722503/150‑1 (MQ=255)
                                                                                                             ttCGCCAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATACCCTCCCTGGCATTTGTTTTATCGACATGGTAAATAATCTCTAAGGTTATTAATAAGAGTTAAAATGTc                             >  2:596829/1‑151 (MQ=255)
                                                                                                              tCGCCAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATACCCTCCCTGGCATTTGTTTTATCGACATGGTAAATAATCTCTAAGGTTATTAATAAGAGTTAAAATGTc                             <  1:602863/150‑1 (MQ=255)
                                                                                                              tCGCCAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATACCCTCCCTGGCATTTGTTTTATCGACATGGTAAATAATCTCTAAGGTTATTAATAAGAGTTAAAATGTc                             <  1:893475/150‑1 (MQ=255)
                                                                                                                 ccAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATACCCTCCCTGGCATTTGTTTTATCGACATGGTAAATAATCTCTAAGGTTATTAATAAGAGTTAAAATGTCACtt                         >  2:733081/1‑151 (MQ=255)
                                                                                                                  cAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATACCCTCCCTGGCATTTGTTTTATCGACATGGTAAATAATCTCTAAGGTTATTAATAAGAGTTAAAATGTCACttt                        >  2:200108/1‑151 (MQ=255)
                                                                                                                  cAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATACCCTCCCTGGCATTTGTTTTATCGACATGGTAAATAATCTCTAAGGTTATTAATAAGAGTTAAAATGTCACttt                        >  1:260438/1‑151 (MQ=255)
                                                                                                                  cAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATACCCTCCCTGGCATTTGTTTTATCGACATGGTAAATAATCTCTAAGGTTATTAATAAGAGTTAAAATGTCACttt                        <  1:82563/151‑1 (MQ=255)
                                                                                                                  cAGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATACCCTCCCTGGCATTTGTTTTATCGACATGGTAAATAATCTCTAAGGTTATTAATAAGAGTTAAAATGTCACttt                        >  1:78478/1‑151 (MQ=255)
                                                                                                                   aGTAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATACCCTCCCTGGCATTTGTTTTATCGACATGGTAAATAATCTCTAAGGTTATTAATAAGAGTTAAAATGTCACTTTg                       <  2:610081/151‑1 (MQ=255)
                                                                                                                    gTAAACTGGCATTTTCATTATGGATGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATACCCTCCCTGGCATTTGTTTTATCGACATGGGAAATAATCTCTAAGGTTATTAATAAGAGTTAAAATGTCACTTTg                       >  2:370651/1‑150 (MQ=255)
                                                                                                                     tAAACTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATACCCTCCCTGGCATTTGTTTTATCGACATGGTAAATAATCTCTAAGGTTATTAATAAGAGTTAAAATGTCACTTTGAt                     >  2:78736/1‑151 (MQ=255)
                                                                                                                      aaaCTGGCATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATACCCTCCCTGGCATTTGTTTTATCGACATGGTAAATAATCTCTAAGGTTATTAATAAGAGTTAAAATGTCACTTTGAt                     >  1:699448/1‑150 (MQ=255)
                                                                                                                             cATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATACCCTCCCTGGCATTTGTTTTATCGACATGGTAAATAATCTCTAAGGTTATTAATAAGAGTTAAAATGTCACTTTGATAATGACGt             <  2:285419/151‑1 (MQ=255)
                                                                                                                             cATTTTCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAAAAATAGCGATATTAATACCCTCCCTGGCATTTGTTTTATCGACATGGTAAATAATATCTAAGGTTATTAATAAGAGATAAAATGTCACATTGATAATg                 >  2:487942/1‑147 (MQ=255)
                                                                                                                                tttCATTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATACCCTCCCTGGCATTTGTTTTATCGACATGGTAAATAATCTCTAAGGTTATTAATAAGAGTTAAAATGTCACTTTGATAATGACGTgg           <  1:623571/150‑1 (MQ=255)
                                                                                                                                    aTTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATACCCTCCCTGGCATTTGTTTTATCGACATGGTAAATAATCTCTAAGGTTATTAATAAGAGTTAAAATGTCACTTTGATAATGACGTGGTTAt       >  2:814425/1‑150 (MQ=255)
                                                                                                                                    aTTATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATACCCTCCCTGGCATTTGTTTTATCGACATGGTAAATAATCTCTAAGGTTATTAATAAGAGTTAAAATGTCACTTTGATAATGACGTGGTTATc      <  2:8774/151‑1 (MQ=255)
                                                                                                                                      tATGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATACCCTCCCTGGCATTTGTTTTATCGACATGGTAAATAATCTCTAAGGTTATTAATAAGAGTTAAAATGTCACTTTGATAATGACGTGGTTATCa     <  1:155900/150‑1 (MQ=255)
                                                                                                                                       aTGGCTGGCGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATACCCTCCCTGGCATTTGTTTTATCGACATGGTAAATAATCTCTAAGGTTATTAATAAGAGTTAAAATGTCACTTTGATAATGACGTGGTTATCAtt   <  2:277940/151‑1 (MQ=255)
                                                                                                                                         ggctggcGCAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATACCCTCCCTGGCATTTGTTTTATCGACATGGTAAATAATCTCTAAGGTTATTAATAAGAGTTAAAATGTCACTTTGATAATGACGTGGTTATCATTa  >  2:535880/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                              
TCGCTGATAATGGTCATTTTTACCGTTGTGCCGCTACTTTGCGCCAGCACTAAGGTATATCCCGCAGATTTAATCGGCAGGTTTTGGCTGATATTTTGCGCCTG‑TTTTTTCGCCAGTAAACTGGCATTTTCATTATGGCTGGCACAGCCACTTAATAATCCTGTAACGCTCAACAATAGCGATATTAATACCCTCCCTGGCATTTGTTTTATCGACATGGTAAATAATCTCTAAGGTTATTAATAAGAGTTAAAATGTCACTTTGATAATGACGTGGTTATCATTA  >  NC_000913/3113260‑3113545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑