Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,118,599	C→T	V172I (GTT→ATT)	sslE ←	putative secreted and surface‑associated lipoprotein mucinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,118,599	0	C	T	100.0%	199.6 / NA	55	V172I (GTT→ATT)	sslE	putative secreted and surface‑associated lipoprotein mucinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/30);  total (25/30)

CCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGCCACGCAAG  >  NC_000913/3118458‑3118742                                                                                                                                              |                                                                                                                                                ccAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCt                                                                                                                                        >  2:812825/1‑151 (MQ=255)   aGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCttt                                                                                                                                      <  1:795280/151‑1 (MQ=255)    gCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCGGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCtttt                                                                                                                                     <  1:888257/151‑1 (MQ=255)    gCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCtttt                                                                                                                                     >  1:832410/1‑151 (MQ=255)    gCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCtttt                                                                                                                                     <  1:273746/151‑1 (MQ=255)        tttGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCtt                                                                                                                                  <  2:844514/150‑1 (MQ=255)         ttGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTg                                                                                                                                 <  2:381972/150‑1 (MQ=255)         ttGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGt                                                                                                                                >  2:803981/1‑151 (MQ=255)           gAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTc                                                                                                                               <  2:613083/150‑1 (MQ=255)                ctTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCg                                                                                                                           <  1:274759/149‑1 (MQ=255)                  tCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGa                                                                                                                         <  1:885501/149‑1 (MQ=255)                    cGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGccc                                                                                                                     <  2:810152/151‑1 (MQ=255)                      gTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGc                                                                                                                   <  1:707592/151‑1 (MQ=255)                        gCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGcc                                                                                                                  >  2:699565/1‑150 (MQ=255)                            gATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTc                                                                                                             >  2:779728/1‑151 (MQ=255)                               cGATTTGCTTATACAGCGAGTCGAAGCGTTTCCTCTCGATCACCGAGGAGAACGTCAGACAACCCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTc                                                                                                             <  1:544544/148‑1 (MQ=255)                                 aTTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTgcg                                                                                                        <  1:477561/151‑1 (MQ=255)                                  tttGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTgcg                                                                                                        >  2:25853/1‑150 (MQ=255)                                    tGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGt                                                                                                     >  1:527449/1‑151 (MQ=255)                                        tataCAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGAAGAACGACAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACAAGCGAAATTGCATTGCTTTTCTTTTCATCGAAGCCCGCCAGTTCTTGCGCGTcc                                                                                                   >  2:714790/1‑149 (MQ=255)                                         ataCAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGCAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTc                                                                                                 >  2:553222/1‑150 (MQ=255)                                         ataCAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCa                                                                                                <  2:758811/151‑1 (MQ=255)                                         ataCAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCa                                                                                                <  1:28428/151‑1 (MQ=255)                                            cAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAgg                                                                                             <  2:308662/151‑1 (MQ=255)                                            cAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAgg                                                                                             >  2:368534/1‑151 (MQ=255)                                               cGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCt                                                                                           >  1:899745/1‑150 (MQ=255)                                                         cGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACtt                                                                                >  2:318450/1‑151 (MQ=255)                                                          gTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACtt                                                                                <  2:206520/150‑1 (MQ=255)                                                             tACTCTCGATCCCCGAGGAGACCGTCAGACAAACCTGTTCTGTATTCGCCGGACACCTGTTATGGGCCGTTACCAGCAAACTCGTTTCGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACttttt                                                                             <  2:153615/150‑1 (MQ=255)                                                              aCTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCa                                                                           <  2:630735/151‑1 (MQ=255)                                                               ctctCGATCACCGAGGCCAACGTCAGACAACCCTGTTCTGTTTTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCTTTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTc                                                                            <  2:66591/149‑1 (MQ=255)                                                                   cGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAAcc                                                                        <  2:358373/149‑1 (MQ=255)                                                                      tCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACcgcac                                                                    <  2:254664/150‑1 (MQ=255)                                                                             ggagAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCt                                                             >  2:80847/1‑150 (MQ=255)                                                                             ggagAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCt                                                             <  2:887125/150‑1 (MQ=255)                                                                                gaACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTAcg                                                          <  2:818894/150‑1 (MQ=255)                                                                                gaACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTAcg                                                          <  1:521380/150‑1 (MQ=255)                                                                                 caCGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTAcgc                                                         >  1:796756/2‑150 (MQ=255)                                                                                         aCAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTct                                                <  1:961/151‑1 (MQ=255)                                                                                            aaCCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTctgac                                             >  2:505067/1‑151 (MQ=255)                                                                                                 gTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAgt                                        <  1:368560/151‑1 (MQ=255)                                                                                                  ttCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAGCGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAg                                         >  2:700199/1‑149 (MQ=255)                                                                                                   tCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAgtg                                       >  1:575789/1‑150 (MQ=255)                                                                                                   tCTGGATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAgtgt                                      >  2:542651/1‑151 (MQ=255)                                                                                                     tGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTg                                    >  2:870252/1‑151 (MQ=255)                                                                                                       tATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGtt                                     <  2:880500/148‑1 (MQ=255)                                                                                                        aTTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAg                                 <  1:203198/151‑1 (MQ=255)                                                                                                              cGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGc                            >  1:57186/1‑150 (MQ=255)                                                                                                                 aCAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAAtt                        <  1:310713/151‑1 (MQ=255)                                                                                                                  cAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTg                       >  1:722842/1‑151 (MQ=255)                                                                                                                          aCTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGtt                >  1:134759/1‑150 (MQ=255)                                                                                                                                 gTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGcc         >  1:431986/1‑150 (MQ=255)                                                                                                                                  ttACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGCCAc       >  2:487923/1‑151 (MQ=255)                                                                                                                                    aCCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGCCACGc     >  1:68071/1‑151 (MQ=255)                                                                                                                                       aGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGCCACGCAAg  <  1:39153/151‑1 (MQ=255)                                                                                                                                              |                                                                                                                                                CCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGCCACGCAAG  >  NC_000913/3118458‑3118742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑