Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,243,428	T→C	E438E (GAA→GAG)	uxaC ←	uronate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,243,428	0	T	C	100.0%	190.8 / NA	53	E438E (GAA→GAG)	uxaC	uronate isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/19);  total (34/19)

ACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCTTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTCAGCAGACCGAGCTGCGCCAGTTGGGTCATCTGACGTTCCA  >  NC_000913/3243280‑3243573                                                                                                                                                     |                                                                                                                                                  aCGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTc                                                                                                                                                 >  1:643805/1‑151 (MQ=255)              cagggttCTTGATGTATTGCATATCAACCCCAGACCTTGTTTCAGTTCAAGGGCGAAGTAGTCCCGCGCATTGTTAACGAAAATTTTTTTCCCCACCCCGCCGAGCGGGTTGTGGTCGGCCGGTGCTTTGCCCGCCTCCACCCAGCGAcc                                                                                                                                     <  2:439034/144‑1 (MQ=255)                       tGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCACCATCTCGCCAAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTgg                                                                                                                           >  2:20668/1‑151 (MQ=255)                           gTATTGCATATCACCCCCAGCCCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCCCCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGcac                                                                                                                        <  1:729104/150‑1 (MQ=255)                                  atatCACCCCCAGTCCTTAGTACAGTTCAATGCCGAAGTAGTCCCGCACATTTTTAAAGCAAATATTTCTCCCCTTCCCGCCCCACAGGTTGATTTCCCCCGGTTCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATgcg                                                                                                                <  1:20690/151‑1 (MQ=255)                                     tCAACCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATgcggcg                                                                                                             >  1:215685/1‑151 (MQ=255)                                      cAACCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGcggcgg                                                                                                            >  2:786914/1‑151 (MQ=255)                                       aaCCCCAGACCAGAGTTCAGTTCAATGGAGAAGTAGTCACGCGCATTGTAAAAGCAAAGATTTTTCACAATCCCGCCCAGCAGGTTAATGCCCGCCGGTGCTCCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATgcggc                                                                                                              <  1:353573/148‑1 (MQ=255)                                        aCCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGa                                                                                                           >  1:799926/1‑150 (MQ=255)                                          cccAGCCCTTAGTTCAGTTCAAGGGCGAAGTAGTCACGCGCATTTTTAAAGCAAATATTTTTCCCCACCCCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGt                                                                                                        <  2:404889/151‑1 (MQ=255)                                           ccAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGt                                                                                                        >  1:818850/1‑150 (MQ=255)                                                      ttcagttcaAGGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTCCCCCACCCCCCCCAGCAGGTTGATGCCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACg                                                                                              <  2:240306/149‑1 (MQ=255)                                                             caATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATg                                                                                      >  2:348705/1‑150 (MQ=255)                                                              aagggCGAAGTATTCCCGCGCATTGTTAAAGAAAATTTTTTCCCCCATCCCGCCCAGCGGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGAc                                                                                    <  2:570062/148‑1 (MQ=255)                                                              aagggCGAAGTAGTCACGCGCATTTTAAAAGAAAATTTTTTCCACCATCCCCCCAAGCAGTTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGAc                                                                                    <  2:186545/148‑1 (MQ=255)                                                              aaTGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGAc                                                                                    >  1:527293/1‑151 (MQ=255)                                                               aTGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGAc                                                                                    >  1:400854/1‑150 (MQ=255)                                                                 ggCGAAGTAGTCACGCGCTTTGTTAAAGAAAATATTTTTCCCCTTCTCCCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAgg                                                                                 <  2:753605/151‑1 (MQ=255)                                                                 ggCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAgg                                                                                 >  2:726731/1‑151 (MQ=255)                                                                      ccgatctaCGCGCATTGTTAAAGCAAATATTTTTCCCCATCCCGCCCAGCAGGTTGATGCCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAg                                                                              <  2:592866/142‑1 (MQ=255)                                                                       gtagtCACGCGCATTGTTAAAGCAAATATTTTCCACCATCTCGCCCAGCAGGTTAATTTCCGCCGGTGTTCCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGaa                                                                               <  1:878082/147‑1 (MQ=255)                                                                             aCGCGCATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAg                                                                              >  1:592915/1‑142 (MQ=255)                                                                               gcgcATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTgtc                                                                   >  2:543070/1‑151 (MQ=255)                                                                                cgcATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTgtc                                                                   >  2:333329/1‑150 (MQ=255)                                                                                 gcATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTgtc                                                                   >  2:740649/1‑149 (MQ=255)                                                                                    ttGTTAAAGCAAATATTTTTCCCCAACTCGCCCAGCAGGTTGATGTCCGCCGGTGCTCCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTgtcag                                                                 <  1:662564/148‑1 (MQ=255)                                                                                       ttAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGc                                                            >  2:599771/1‑150 (MQ=255)                                                                                        tAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCAt                                                          >  1:329969/1‑151 (MQ=255)                                                                                        tAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCAt                                                          >  2:417613/1‑151 (MQ=255)                                                                                         aaaGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCAt                                                          >  1:778000/1‑150 (MQ=255)                                                                                              aaaTATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGAc                                                    >  1:391088/1‑151 (MQ=255)                                                                                                atatTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGAc                                                    >  2:56302/1‑149 (MQ=255)                                                                                                 tatTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACCGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGa                                                     >  2:562628/1‑147 (MQ=255)                                                                                                  atTTTTCACCATCTCGCCCAGCAGGGTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGAGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACa                                                   >  1:178301/1‑148 (MQ=255)                                                                                                    ttttCCCCATCCCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGc                                               <  1:870856/150‑1 (MQ=255)                                                                                                     tttCCCCATCCCCCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCg                                              <  1:516321/150‑1 (MQ=255)                                                                                                     tttCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCgg                                             >  2:486992/1‑151 (MQ=255)                                                                                                      ttCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGc                                            >  2:861131/1‑151 (MQ=255)                                                                                                      ttCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGc                                            >  2:868165/1‑151 (MQ=255)                                                                                                      ttCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGAGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGc                                            >  1:139899/1‑151 (MQ=255)                                                                                                          ccaccccccccAGCAGGTTGATGTCGGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCt                                           <  2:853780/140‑1 (MQ=255)                                                                                                          ccATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTc                                          >  2:803913/1‑149 (MQ=255)                                                                                                              ctcGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTcagc                                       >  2:175141/1‑148 (MQ=255)                                                                                                              cccccccGGCGGGTTGATGCCCCCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTcagca                                      <  2:2868/145‑1 (MQ=255)                                                                                                                   ccAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTCAGCAGACCg                                 >  2:183629/1‑149 (MQ=255)                                                                                                                    cagcagGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTCAGCAGACCGAGc                              >  1:876278/1‑151 (MQ=255)                                                                                                                        agGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTCAGCAGACCGAGCTgcg                          >  1:535006/1‑151 (MQ=255)                                                                                                                         ggTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTCAGCAGACCGAGCTgcgc                         >  1:402973/1‑151 (MQ=255)                                                                                                                              aTGCCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTCAGCAGACCGAGCTGCGCCAGt                     <  1:778519/150‑1 (MQ=255)                                                                                                                               tGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTCAGCAGAACGAGCTGCGCCAGtt                    >  2:553779/1‑150 (MQ=255)                                                                                                                                 tCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTCAGCAGACCGAGCTGCGCCAGTTggg                 >  1:663508/1‑151 (MQ=255)                                                                                                                                                cccGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTCAGCAGACCGAGCTGCGCCAGTTGGGTCATCTGACGtt     <  1:15534/148‑1 (MQ=255)                                                                                                                                                cccGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTCAGCAGACCGAGCTGCGCCAGTTGGGTCATCTGACGTTCCa  <  2:564349/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                  ACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCTTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTCAGCAGACCGAGCTGCGCCAGTTGGGTCATCTGACGTTCCA  >  NC_000913/3243280‑3243573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑