Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,244,516	T→C	T76A (ACC→GCC)	uxaC ←	uronate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,244,516	0	T	C	100.0%	179.3 / NA	50	T76A (ACC→GCC)	uxaC	uronate isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/23);  total (27/23)

GCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAATCGGCTG  >  NC_000913/3244371‑3244663                                                                                                                                                  |                                                                                                                                                    gCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCacac                                                                                                                                                 >  1:802431/1‑150 (MQ=255)    gTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGAc                                                                                                                                             <  2:229512/151‑1 (MQ=255)       gACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGc                                                                                                                                           >  2:713316/1‑150 (MQ=255)          ggAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCgg                                                                                                                                       <  1:856308/151‑1 (MQ=255)              aaaGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCcac                                                                                                                                   >  1:310749/1‑151 (MQ=255)                      ttGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTgg                                                                                                                           >  1:276016/1‑151 (MQ=255)                      ttGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGACGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTgg                                                                                                                           >  1:883184/1‑151 (MQ=255)                         ccAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTAc                                                                                                                        >  2:113372/1‑151 (MQ=255)                         ccAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCCCGGCCACACCGTTGGTAc                                                                                                                        >  2:149452/1‑151 (MQ=255)                            gTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCa                                                                                                                     <  2:742470/151‑1 (MQ=255)                                 aCCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAgc                                                                                                                 <  1:615815/150‑1 (MQ=255)                                        ggacggCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCAtt                                                                                                         <  1:854799/151‑1 (MQ=255)                                          acggCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTg                                                                                                       <  1:577431/151‑1 (MQ=255)                                                    tCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGc                                                                                             >  2:745543/1‑151 (MQ=255)                                                     cGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGcc                                                                                            >  1:151301/1‑151 (MQ=255)                                                       aGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCtt                                                                                          >  1:370922/1‑151 (MQ=255)                                                           gCGTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCa                                                                                       <  2:706312/150‑1 (MQ=255)                                                             gTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGcc                                                                                    <  1:177809/151‑1 (MQ=255)                                                               ccAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGcc                                                                                    >  1:746838/1‑149 (MQ=255)                                                               ccAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCa                                                                                   <  1:55986/150‑1 (MQ=255)                                                               ccAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAg                                                                                  <  2:66142/151‑1 (MQ=255)                                                                      tataACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCAt                                                                           >  1:348909/1‑151 (MQ=255)                                                                        taACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATAc                                                                         >  2:61103/1‑151 (MQ=255)                                                                         aaCGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATAc                                                                         >  2:861530/1‑150 (MQ=255)                                                                            gggTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGt                                                                     <  2:83404/151‑1 (MQ=255)                                                                             ggTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGtt                                                                    >  1:244622/1‑151 (MQ=255)                                                                             ggTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGtt                                                                    >  2:528645/1‑151 (MQ=255)                                                                             ggTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGtt                                                                    >  1:642136/1‑151 (MQ=255)                                                                               ttGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGtttt                                                                  >  1:160472/1‑151 (MQ=255)                                                                                tGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCTTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGtttt                                                                  <  2:588936/150‑1 (MQ=255)                                                                                   cGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTaaa                                                              <  1:581920/151‑1 (MQ=255)                                                                                   cGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTaaa                                                              <  1:231842/151‑1 (MQ=255)                                                                                     aTAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAAc                                                            >  1:351946/1‑151 (MQ=255)                                                                                      tAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACg                                                           >  1:546253/1‑151 (MQ=255)                                                                                       aGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACg                                                           <  2:392270/150‑1 (MQ=255)                                                                                        gtgtGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGAt                                                         <  1:571997/151‑1 (MQ=255)                                                                                                aaCAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTcc                                                 >  1:703102/1‑151 (MQ=255)                                                                                                  cAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGc                                               <  2:684326/151‑1 (MQ=255)                                                                                                     tCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAAt                                            >  2:400326/1‑151 (MQ=255)                                                                                                                    cAAATTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCa                              >  2:424660/1‑150 (MQ=255)                                                                                                                     aaaTTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCaa                             >  1:322969/1‑150 (MQ=255)                                                                                                                       aTTTTTCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATg                          >  2:776310/1‑151 (MQ=255)                                                                                                                         ttttCACGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATgg                         <  1:766668/150‑1 (MQ=255)                                                                                                                              aCGGTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATgg                   <  2:263827/151‑1 (MQ=255)                                                                                                                                ggTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGTTAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGt                  >  1:669134/1‑150 (MQ=255)                                                                                                                                ggTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTa                 <  2:746788/151‑1 (MQ=255)                                                                                                                                 gTCAGACGCATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTaa                <  1:440510/151‑1 (MQ=255)                                                                                                                                         cATCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAAt        <  1:889510/151‑1 (MQ=255)                                                                                                                                          aTCACCGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAATc       <  2:322943/151‑1 (MQ=255)                                                                                                                                               cGGCACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAATCGGCTg  >  1:705670/1‑151 (MQ=255)                                                                                                                                                  |                                                                                                                                                    GCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAATCGGCTG  >  NC_000913/3244371‑3244663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑