Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,257,613	G→A	A133V (GCC→GTC)	yhaO ←	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,257,613	0	G	A	100.0%	213.5 / NA	56	A133V (GCC→GTC)	yhaO	putative transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/25);  total (31/25)

GGTCGAAATTTTGAATAACAATTTCTCGCCGCGTGAGGAGATCGCCACCAGAATGCAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATC  >  NC_000913/3257465‑3257760                                                                                                                                                     |                                                                                                                                                    ggTCGAAATTTTGAATAACAATTTCTCGCCGCGTGAGGAGATCGCCACCAGAATGCAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACg                                                                                                                                                   <  1:574666/151‑1 (MQ=255)       aaTTTTGAATAACAATTTCTCGCCGCGTGAGGAGATCGCCACCAGAATGCAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGa                                                                                                                                              >  2:518987/1‑150 (MQ=255)          tttGAATAACAATTTCTCGCCGCGTGAGGAGATCGCCACCAGAATGCAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATa                                                                                                                                           <  1:357624/150‑1 (MQ=255)             gAATAACAATTTCTCGCCGCGTGAGGAGATCGCCACCAGAATGCAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGAc                                                                                                                                        <  2:494538/150‑1 (MQ=255)                  aCAATTTCTCGCCGCGTGAGGAGATCGCCACCAGAATGCAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACAt                                                                                                                                  <  1:333665/151‑1 (MQ=255)                        tctcGCCGCGTGAGGAGATCGCCACCAGAATGCAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCaa                                                                                                                              <  2:66146/149‑1 (MQ=255)                            gccgcGTGAGGAGATCGCCACCAGAATGCAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCAcc                                                                                                                        >  1:849819/1‑151 (MQ=255)                                cgTGAGGAGATCGCCACCAGAATGCAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGc                                                                                                                     >  1:729967/1‑150 (MQ=255)                                cgTGAGGAGATCGCCACCAGAATGCAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCa                                                                                                                    <  2:113122/151‑1 (MQ=255)                                 gTGAGGAGATCGCCACCAGAATGCAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCAt                                                                                                                   >  2:590456/1‑151 (MQ=255)                                  tGAGGAGATCGCCACCAGAATGCAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCAtt                                                                                                                  >  2:540872/1‑151 (MQ=255)                                    aggagATCGCCACCAGAATGCAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTAc                                                                                                                <  2:292720/151‑1 (MQ=255)                                        gaTCGCCACCAGAATGCAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGaaa                                                                                                            <  2:93144/151‑1 (MQ=255)                                         aTCGCCACCAGAATGCAAATCAGTACCAGACCATAAAAGGGACTGTCTAGCAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGAGGACGGTGGAATAGACGAACATCCAAATCACCATCATGACGaaa                                                                                                            <  2:682767/150‑1 (MQ=255)                                              caccaGAATGCAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAgc                                                                                                      <  1:599951/151‑1 (MQ=255)                                              caccaGAATGCAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAgc                                                                                                      >  2:138374/1‑151 (MQ=255)                                               accaGAATGCAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAgcg                                                                                                     <  2:26053/151‑1 (MQ=255)                                                  aGAATGCAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCAc                                                                                                  >  1:440076/1‑151 (MQ=255)                                                    aaTGCAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCt                                                                                                <  1:105567/151‑1 (MQ=255)                                                      tGCAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTa                                                                                               >  2:433766/1‑150 (MQ=255)                                                      tGCAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACATa                                                                                               >  2:325968/1‑150 (MQ=255)                                                           aTCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAgg                                                                                          >  1:815820/1‑150 (MQ=255)                                                               gTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGcc                                                                                     >  2:139528/1‑151 (MQ=255)                                                                tACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGccc                                                                                    >  1:559212/1‑151 (MQ=255)                                                                    aGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAg                                                                                 <  2:413419/150‑1 (MQ=255)                                                                         aTAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGttttt                                                                            >  1:364306/1‑150 (MQ=255)                                                                           aaaaGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTAcc                                                                         >  1:566861/1‑151 (MQ=255)                                                                           aaaaGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTAcc                                                                         >  1:833620/1‑151 (MQ=255)                                                                            aaaGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCt                                                                        <  2:399079/151‑1 (MQ=255)                                                                                 gACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAAt                                                                    >  2:101822/1‑150 (MQ=255)                                                                                  aCTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATa                                                                   >  1:39867/1‑150 (MQ=255)                                                                                       cTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCt                                                             >  2:740436/1‑151 (MQ=255)                                                                                           cAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATg                                                         >  2:774574/1‑151 (MQ=255)                                                                                            aGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATg                                                         >  1:687189/1‑150 (MQ=255)                                                                                            aGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGa                                                        <  1:288525/151‑1 (MQ=255)                                                                                              cagcccTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCTCCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGAc                                                       <  1:45891/147‑1 (MQ=255)                                                                                                      ccGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTa                                              <  1:589502/151‑1 (MQ=255)                                                                                                        gtaaCGCCGAAGGAATGCAGGAAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATACAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGACGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGt                                               >  2:566551/4‑148 (MQ=255)                                                                                                          cACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCt                                          >  1:312565/1‑151 (MQ=255)                                                                                                            cGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCttt                                        <  2:248982/151‑1 (MQ=255)                                                                                                            cGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCttt                                        >  2:462740/1‑151 (MQ=255)                                                                                                            cGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCtt                                         <  2:193708/150‑1 (MQ=255)                                                                                                             gCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCttt                                        >  1:99369/1‑150 (MQ=255)                                                                                                                gAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACAt                                    >  2:238456/1‑151 (MQ=255)                                                                                                                   ggTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCt                                 >  2:136749/1‑151 (MQ=255)                                                                                                                      aTGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGt                              >  2:28930/1‑151 (MQ=255)                                                                                                                       tGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTg                             >  1:115901/1‑151 (MQ=255)                                                                                                                        gCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTg                             <  1:326194/150‑1 (MQ=255)                                                                                                                            gTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCt                        <  1:9020/151‑1 (MQ=255)                                                                                                                              aGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTg                       <  1:433813/150‑1 (MQ=255)                                                                                                                                gAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGcc                     >  1:487758/1‑150 (MQ=255)                                                                                                                                        aTCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTAtt             >  1:568143/1‑150 (MQ=255)                                                                                                                                           gTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATtaata         <  1:139551/151‑1 (MQ=255)                                                                                                                                               gTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAAc      >  2:867666/1‑150 (MQ=255)                                                                                                                                               gTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACa     <  1:196825/151‑1 (MQ=255)                                                                                                                                                  aTGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATc  <  2:437586/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    GGTCGAAATTTTGAATAACAATTTCTCGCCGCGTGAGGAGATCGCCACCAGAATGCAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATC  >  NC_000913/3257465‑3257760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑