Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	396,736	A→G	D33G (GAC→GGC)	sbmA →	peptide antibiotic transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	396,736	0	A	G	100.0%	173.8 / NA	49	D33G (GAC→GGC)	sbmA	peptide antibiotic transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/20);  total (29/20)

CGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGTTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACGCTTACTACATTGTTTGCGTAGGACTTTTTGCATTGTTCTGGTTTATCTACAGCCCGC  >  NC_000913/396588‑396879                                                                                                                                                     |                                                                                                                                                cGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGGCt                                                                                                                                               >  1:73022/1‑151 (MQ=255)  ggTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGGCTg                                                                                                                                              >  2:264900/1‑151 (MQ=255)      aaTACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGGCTGGGTg                                                                                                                                          <  1:346215/151‑1 (MQ=255)       aTACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGGCTGGGTgg                                                                                                                                         >  2:589661/1‑151 (MQ=255)         acacAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGGCTGGGTGgcg                                                                                                                                       <  1:61205/151‑1 (MQ=255)          cacaGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGGCTGGGTGgcgc                                                                                                                                      >  2:157385/1‑151 (MQ=255)                  ttgttAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGGCTGGGTGGCGCGTATCAcc                                                                                                                              >  1:552317/1‑151 (MQ=255)                  ttgttAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGGCTGGGGGGCGCGTATCAcc                                                                                                                              >  1:637838/1‑151 (MQ=255)                    gttAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGGCTGGGTGGCGCGTATCACCg                                                                                                                             <  1:669499/150‑1 (MQ=255)                       aaaaCGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGGCTGGGTGGCGCGTATCACCGgcgc                                                                                                                         >  1:634478/1‑151 (MQ=255)                          aCGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGGCTGGGTGGCGCGTATCACCGGCGCTTc                                                                                                                      >  2:679177/1‑151 (MQ=255)                            gATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGGCTGGGTGGCGCGTATCACCGGCGCTTCCg                                                                                                                    <  1:589710/151‑1 (MQ=255)                              tAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGGCTGGGTGGCGCGTATCACCGGCGCTTCCggg                                                                                                                  >  1:669877/1‑151 (MQ=255)                               aagaagTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGGCTGGGTGGCGCGTATCACCGGCGCTTCCggg                                                                                                                  <  1:508002/150‑1 (MQ=255)                               aagaagTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGGCTGGGTGGCGCGTATCACCGGCGCTTCCGGGc                                                                                                                 >  2:432260/1‑151 (MQ=255)                                   agTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGGCTGGGTGGCGCGTATCACCGGCGCTTCCGGGc                                                                                                                 >  2:380949/1‑147 (MQ=255)                                     ttAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGGCTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATcc                                                                                                           >  2:224278/1‑151 (MQ=255)                                      tAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCTTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGGCTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATccc                                                                                                          >  2:307055/1‑151 (MQ=255)                                       aGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGGCTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGAGccc                                                                                                          <  1:233465/150‑1 (MQ=255)                                                 cATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGGCTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGcgccg                                                                                               >  2:24232/1‑151 (MQ=255)                                                               ttttttCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGGCTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCg                                                                                 >  2:565381/1‑151 (MQ=255)                                                                tttttCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGGCTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTc                                                                                  <  2:439504/149‑1 (MQ=255)                                                                tttttCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGGCTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTc                                                                                  <  2:368830/149‑1 (MQ=255)                                                                  tttCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGGCTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGtt                                                                               <  1:484199/150‑1 (MQ=255)                                                                  tttCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGGCTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTg                                                                              >  1:483611/1‑151 (MQ=255)                                                                    tCCCAAAGCCTGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGGCTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTgg                                                                             <  2:489097/150‑1 (MQ=255)                                                                    tCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGGCTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTgg                                                                             >  2:243286/1‑150 (MQ=255)                                                                        aaaGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGGCTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTc                                                                        >  2:708059/1‑151 (MQ=255)                                                                        aaaGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGGCTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTc                                                                        >  1:884720/1‑151 (MQ=255)                                                                             cGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGGCTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGAt                                                                   <  2:409800/151‑1 (MQ=255)                                                                               ggAACGTTTTTTCTCTCGGTCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGGCTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGAttt                                                                 >  2:285418/1‑151 (MQ=255)                                                                                 aaCGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGGCTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGAttttt                                                               >  1:302303/1‑151 (MQ=255)                                                                                      tttttCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGGCTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACg                                                           <  2:263326/150‑1 (MQ=255)                                                                                      tttttCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGGCTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACGc                                                          >  1:849527/1‑151 (MQ=255)                                                                                       ttttCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGGCTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACGCt                                                         >  1:16462/1‑151 (MQ=255)                                                                                        tttCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGGCTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACGCt                                                         <  1:457879/150‑1 (MQ=255)                                                                                            tctcGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGGCTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACGCTtact                                                     >  2:671192/1‑150 (MQ=255)                                                                                              tcGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGGCTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACGCTtactac                                                   >  1:44778/1‑150 (MQ=255)                                                                                               cGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGGCTGGGTGGTGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACGCTtacta                                                    <  1:511275/148‑1 (MQ=255)                                                                                               cGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGGCTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACGCTTACTACAt                                                 >  1:49910/1‑151 (MQ=255)                                                                                                    ttCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGGCTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACGCTTACTACATTGttt                                            >  2:381136/1‑151 (MQ=255)                                                                                                              cATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGGCTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACGCTTACTACATTGTTTGCGTAGGACt                                  >  1:401509/1‑151 (MQ=255)                                                                                                                  gattgCCGTTATCTTCTGGCAAGCCGGTGGGGGTGGCTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACGCTTACTACATTGTTTGCGTAGGACttttt                              <  1:533581/151‑1 (MQ=255)                                                                                                                  gattgCCGTTATCTTCTGGCAAGCCGGTGGGGGTGGCTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACGCTTACTACATTGTTTGCGTAGGACtttt                               <  2:473001/150‑1 (MQ=255)                                                                                                                      gCCGTTATCTTCTGGCAAGCCGGTGGGGGTGGCTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACGCTTACTACATTGTTTGCGTAGGACTTTTTGCAt                          <  1:640605/151‑1 (MQ=255)                                                                                                                       ccGTTATCTTCTGGCAAGCCGGTGGGGGTGGCTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACGCTTACTACATTGTTTGCGTAGGACTTTTTGCAtt                         <  2:631243/151‑1 (MQ=255)                                                                                                                           tATCTTCTGGCAAGCCGGTGGGGGTGGCTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACGCTTACTACATTGTTTGCGTAGGACTTTTTGCATTGt                       <  2:851085/149‑1 (MQ=255)                                                                                                                                        gacGGTGGGGGTGGCTGGGTGGCGCGTATCGCCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACGCTTACTACATTGTTTGCGTAGGACTTTTTGCATTGTTCTGGTTTATCt           <  2:75235/146‑1 (MQ=255)                                                                                                                                              gggggTGGCTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACGCTTACTACATTGTTTGCGTAGGACTTTTTGCATTGTTCTGGTTTATCTACAGCCCGc  >  2:260408/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                CGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGTTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACGCTTACTACATTGTTTGCGTAGGACTTTTTGCATTGTTCTGGTTTATCTACAGCCCGC  >  NC_000913/396588‑396879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑