Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,286,334	T→C	L22P (CTT→CCT)	agaI →	galactosamine‑6‑phosphate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,286,334	0	T	C	100.0%	186.3 / NA	52	L22P (CTT→CCT)	agaI	galactosamine‑6‑phosphate isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/31);  total (21/31)

GGGTGAAAAAAGCGCATCCGGTGCTGTTAATCGGCGTGACTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCTTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTACTGACGTATCATTATCTGGTAGAAAAAATC  >  NC_000913/3286187‑3286479                                                                                                                                                    |                                                                                                                                                  gagTGAAAAGAACTCATCCGGTGCTGTTAATCGGCGTGCCTTTTGTGCTCTCTATTGTTTGTACCGCATTCGGCATTTTGTAAATGGAACGATGCACTGTATCTGGTGGTGGCTCTTTACTTAAGGAAATTCATCCTGTGCATACCCCTCa                                                                                                                                                <  2:51625/149‑1 (MQ=255)      aaaaaaGCGCATCCGGTGCTGTTAATCGGCGTGACTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAg                                                                                                                                           <  1:189953/151‑1 (MQ=255)      aaaaaaGCGCATCCGGTGCTGTTAATCGGCGTGACTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAg                                                                                                                                           <  1:625739/151‑1 (MQ=255)        aaaaGCGCATCCGGTGCTGTTAATCGGCGTGACTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGtt                                                                                                                                         <  2:79542/151‑1 (MQ=255)          aaGCGCATCCGGTGCTGTTAATCGGCGTGACTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTg                                                                                                                                        >  2:322648/1‑150 (MQ=255)             cgcATCCGGTGCTGTTAATCGGCGTGACTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGaaaa                                                                                                                                    <  1:863061/151‑1 (MQ=255)                aTCCGGTGCTGTTAATCGGCGTGACTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACta                                                                                                                                 <  2:614073/151‑1 (MQ=255)                        cTGTTAATCGGCGTGACTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCg                                                                                                                          <  2:517465/150‑1 (MQ=255)                        cTGTTAATCGGCGTGACTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGt                                                                                                                         >  2:23442/1‑151 (MQ=255)                          gTTAATCGGCGTGACTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGt                                                                                                                         <  1:23464/149‑1 (MQ=255)                             aaTCGGCGTGACTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGt                                                                                                                    <  2:77803/151‑1 (MQ=255)                                   cGTGACTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGt                                                                                                              <  2:736678/151‑1 (MQ=255)                                   cGTGACTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGt                                                                                                              >  1:859100/1‑151 (MQ=255)                                      gACTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTTCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGc                                                                                                            <  2:811399/150‑1 (MQ=255)                                       aCTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCa                                                                                                          <  2:570186/151‑1 (MQ=255)                                           ttGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCg                                                                                                       <  2:808572/150‑1 (MQ=255)                                             gtgCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAat                                                                                                    <  1:248461/151‑1 (MQ=255)                                                        tgtttgttCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGc                                                                                          <  2:468356/150‑1 (MQ=255)                                                         gtttgttCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGcc                                                                                         <  1:75661/150‑1 (MQ=255)                                                             gttCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGAt                                                                                    <  2:808388/151‑1 (MQ=255)                                                             gttCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGAt                                                                                    >  2:660628/1‑151 (MQ=255)                                                               tCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATc                                                                                   <  1:864132/150‑1 (MQ=255)                                                                 cGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGt                                                                                >  2:467403/1‑151 (MQ=255)                                                                  gCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCg                                                                                 >  2:272006/1‑149 (MQ=255)                                                                    aTTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGc                                                                             >  1:434718/1‑151 (MQ=255)                                                                    aTTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGATGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGc                                                                             >  2:411192/1‑151 (MQ=255)                                                                        ggCATTTTGTAAATGGAACGAGGCACTGGGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTTAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCaaa                                                                          >  2:42774/1‑150 (MQ=255)                                                                        ggCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAAc                                                                         <  1:422537/151‑1 (MQ=255)                                                                           aTTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCg                                                                       >  1:80909/1‑150 (MQ=255)                                                                              ttGTAAATGGAACGAGGCACTGTGTCTGGAGGTGCCTCTTTATTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTGTAAAACTATATGTCGTTAACTGTACGTGCTAGCGAATATTTATTGGCGGTGATACGTAGCaaatctcatg                                                                   >  2:244875/1‑144 (MQ=255)                                                                                       gAACGAGGCACTGCGTCTGGTGGTGCCTCTTTAATTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGAttt                                                          >  2:648955/1‑151 (MQ=255)                                                                                        aaCGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGAt                                                            >  2:717074/1‑148 (MQ=255)                                                                                           gAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCt                                                      >  2:308375/1‑151 (MQ=255)                                                                                           gAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCt                                                      <  1:184528/151‑1 (MQ=255)                                                                                           gAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCt                                                      >  2:853884/1‑151 (MQ=255)                                                                                                        tggtggTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGcc                                         >  2:601754/1‑151 (MQ=255)                                                                                                         ggtggtGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAg                                           <  2:865070/148‑1 (MQ=255)                                                                                                            ggtgCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACg                                      <  1:660677/150‑1 (MQ=255)                                                                                                              tgCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGcc                                    >  1:636577/1‑150 (MQ=255)                                                                                                                ccTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCAtt                                 <  1:711152/151‑1 (MQ=255)                                                                                                                    tttACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTACt                              >  1:329107/1‑150 (MQ=255)                                                                                                                     ttACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTACTg                             <  1:903723/150‑1 (MQ=255)                                                                                                                       aCTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTACTGACg                          >  1:161320/1‑151 (MQ=255)                                                                                                                         ttAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTACTGACGt                         >  1:19312/1‑150 (MQ=255)                                                                                                                            aGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTACTGACGTATCa                     <  2:504456/151‑1 (MQ=255)                                                                                                                            aGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTACTGACGTATCa                     <  1:469039/151‑1 (MQ=255)                                                                                                                             ggAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTACTGACGTATCAt                    <  1:563567/151‑1 (MQ=255)                                                                                                                                aTTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTACTGACGTATCATTAt                 <  1:843418/151‑1 (MQ=255)                                                                                                                                  ttCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTACTGACGTATCATTATc                <  1:469474/150‑1 (MQ=255)                                                                                                                                         tgtgCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTACTGACGTATCATTATCTGGTAGa         >  2:681208/1‑150 (MQ=255)                                                                                                                                            gCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTACTGACGTATCATTATCTGGTAGaaaaa     <  2:735951/151‑1 (MQ=255)                                                                                                                                               aaCCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTACTGACGTATCATTATCTGGTAGAAAAAATc  <  1:156255/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                  GGGTGAAAAAAGCGCATCCGGTGCTGTTAATCGGCGTGACTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCTTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTACTGACGTATCATTATCTGGTAGAAAAAATC  >  NC_000913/3286187‑3286479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑