Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,296,292	C→T	R162C (CGC→TGC)	diaA →	DnaA initiator‑associating factor for replication initiation

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,296,292	0	C	T	100.0%	245.2 / NA	65	R162C (CGC→TGC)	diaA	DnaA initiator‑associating factor for replication initiation
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/30);  total (35/30)

AGCCATTTCCACCCGTGGCAACAGCCGCGATATTGTTAAAGCAGTTGAAGCCGCCGTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTT  >  NC_000913/3296150‑3296438                                                                                                                                               |                                                                                                                                                   aGCCATTTCCACCCGTGGCAACAGCCGCGATATTGTTAAAGCAGTTGAAGCCGCCGTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTcc                                                                                                                                             <  2:184213/150‑1 (MQ=255) aGCCATTTCCACCCGTGGCAACAGCCGCGATATTGTTAAAGCAGTTGAAGCCGCCGTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTcc                                                                                                                                             <  1:626470/150‑1 (MQ=255)    cATTTCCCCCCGTGGCAACAGCCGCGATATTGTTAAAGCAGTTGAAGCCGCCGTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCg                                                                                                                                         <  1:484034/151‑1 (MQ=255)    cATTTCCACCCGTGGCAACAGCCGCGATATTGTTAAAGCAGTTGAAGCCGCCGTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCg                                                                                                                                         >  1:826901/1‑151 (MQ=255)       tcccACCCGGGGCAACAGCCGCGATATTGTTAAAGCAGTTGAAGCCGCCGTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCa                                                                                                                                       <  2:872102/148‑1 (MQ=255)         ccccccGTGGCAACAGCCGCGATATTGTTAAAGCGGTGGAAGCCGCCGTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCg                                                                                                                                    <  2:688734/148‑1 (MQ=255)           aCCCGTGGCAACAGCCGCGATATTGTTAAAGCAGTTGAAGCCGCCGTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGt                                                                                                                                   >  2:527635/1‑150 (MQ=255)             ccGTGGCAACAGCCGCGATATTGTTAAAGCAGTTGAAGCCGCCGTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGt                                                                                                                                >  2:421239/1‑151 (MQ=255)                tGGCAACAGCCGCGATATTGTTAAAGCAGTTGAAGCCGCCGTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGACGTGGAGATCTGCATTCCTTCGCATCGTAGTGCt                                                                                                                             >  2:191884/1‑151 (MQ=255)                    aaCAGCCGCGATATTGTTAAAGCAGTTGAAGCCGCCGTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCa                                                                                                                         <  1:117289/151‑1 (MQ=255)                       aGCCGCGATATTGTTAAAGCAGTTGAAGCCGCCGTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTc                                                                                                                      >  2:212291/1‑151 (MQ=255)                          cgcgATATTGTTAAAGCAGTTGAAGCCGCCGTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAgg                                                                                                                   <  1:102106/151‑1 (MQ=255)                                atTGTTAAAGCAGTTGAAGCCGCCGTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGGTAGGGCCACATGATGGGGAGATCTTCATTCCTTCGCATCGCAGTGCGCGCATTCAGGAAATGc                                                                                                             >  2:96896/1‑151 (MQ=255)                                     tAAAGCAGTTGAAGCCGCCGTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATg                                                                                                        >  2:201350/1‑151 (MQ=255)                                      aaaGCAGTTGAAGCCGCCGTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGc                                                                                                       >  1:666824/1‑151 (MQ=255)                                       aaGCAGTTGAAGCCGCCGTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCt                                                                                                      >  2:635477/1‑151 (MQ=255)                                         gCAGTTGAAGCAGCCGTTACGCGTGATATGACCATTGTGGCATTTACCGGCTTTGACGGCGGCGGACTTGCAGGTTTGTTAGGGCCCCAGGATGTGGATATCTGCATTGCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTg                                                                                                     >  2:601227/1‑150 (MQ=255)                                           aGTTGAAGCCGCCGTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGAc                                                                                                   <  1:786936/150‑1 (MQ=255)                                           aGTTGAAGCCGCCGTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACg                                                                                                  >  1:202426/1‑151 (MQ=255)                                               gAAGCCGCCGTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATACTGACGGTaa                                                                                              <  2:759190/151‑1 (MQ=255)                                                  gccgccGTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAAtt                                                                                           >  1:415876/1‑151 (MQ=255)                                                  gccgccGTTACGCGTGATATGACCACTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAAtt                                                                                           >  2:63225/1‑151 (MQ=255)                                                   ccgccgTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTg                                                                                          <  2:650653/151‑1 (MQ=255)                                                       cgTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGACCTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTCCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGGAAAtt                                                                                           >  1:507023/1‑146 (MQ=255)                                                       cgTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCtg                                                                                      <  2:764253/151‑1 (MQ=255)                                                        gTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCtgt                                                                                     <  2:239075/151‑1 (MQ=255)                                                         ttACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCtgtg                                                                                    >  1:61516/1‑151 (MQ=255)                                                              cgTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATc                                                                               >  2:794414/1‑151 (MQ=255)                                                               gTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCt                                                                              <  1:908678/151‑1 (MQ=255)                                                                tGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTG‑TAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTg                                                                             >  2:794044/1‑150 (MQ=255)                                                                    atGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCg                                                                         >  2:811630/1‑151 (MQ=255)                                                                       aCCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATa                                                                      <  2:229473/151‑1 (MQ=255)                                                                           ttGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAacac                                                                  >  1:630755/1‑151 (MQ=255)                                                                               ggCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCtt                                                              >  1:397699/1‑151 (MQ=255)                                                                                    tGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTccc                                                         >  2:453218/1‑151 (MQ=255)                                                                                     gACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCt                                                        >  2:885547/1‑151 (MQ=255)                                                                                     gACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCt                                                        <  2:477576/151‑1 (MQ=255)                                                                                        cGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCAc                                                     >  2:391545/1‑151 (MQ=255)                                                                                           cTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCAcc                                                    >  2:710296/1‑149 (MQ=255)                                                                                            tATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAgg                                                 >  2:516410/1‑151 (MQ=255)                                                                                             aTGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAgg                                                 >  1:906792/1‑150 (MQ=255)                                                                                             aTGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAg                                                  <  2:722728/149‑1 (MQ=255)                                                                                                 cggcggCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGgatgat                                            <  2:404500/151‑1 (MQ=255)                                                                                                   gcggcgAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTa                                          <  1:687198/151‑1 (MQ=255)                                                                                                   gcggcgAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTa                                          <  2:263288/151‑1 (MQ=255)                                                                                                      gcgAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAgg                                       <  2:397673/151‑1 (MQ=255)                                                                                                        gAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAgg                                       >  2:406668/1‑149 (MQ=255)                                                                                                         aaCTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGgaga                                    <  2:574999/151‑1 (MQ=255)                                                                                                            ttGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAAt                                  >  2:517436/1‑150 (MQ=255)                                                                                                            ttGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAAt                                  >  2:856632/1‑150 (MQ=255)                                                                                                             tGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATa                                 <  1:33320/150‑1 (MQ=255)                                                                                                               cAGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACAt                              >  2:106806/1‑151 (MQ=255)                                                                                                                aGGTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATg                             <  2:462144/151‑1 (MQ=255)                                                                                                                 ggTTTGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATg                             >  2:107512/1‑150 (MQ=255)                                                                                                                     tGTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGc                        >  2:824449/1‑151 (MQ=255)                                                                                                                      gTTAGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCa                       <  2:783503/151‑1 (MQ=255)                                                                                                                         aGGGCCACAGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCAtt                     <  1:404971/150‑1 (MQ=255)                                                                                                                                 aGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACTGTAAATTGCCTGTGCGATCTGATCGATAACCCGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAAt            <  2:476030/151‑1 (MQ=255)                                                                                                                                 aGGATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAAt            >  1:897015/1‑151 (MQ=255)                                                                                                                                   gATGTGGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCg          >  1:516318/1‑151 (MQ=255)                                                                                                                                      gtgGAGATCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCa        <  2:43449/150‑1 (MQ=255)                                                                                                                                         gagaTCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTcc    <  1:66197/151‑1 (MQ=255)                                                                                                                                         gagaTCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTcc    <  2:641041/151‑1 (MQ=255)                                                                                                                                          agaTCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCt   <  2:490811/151‑1 (MQ=255)                                                                                                                                           gaTCTGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCtt  >  1:108431/1‑151 (MQ=255)                                                                                                                                               |                                                                                                                                                   AGCCATTTCCACCCGTGGCAACAGCCGCGATATTGTTAAAGCAGTTGAAGCCGCCGTTACGCGTGATATGACCATTGTGGCATTGACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTT  >  NC_000913/3296150‑3296438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑