Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,311,736	G→A	intergenic (‑52/+97)	rpsO ← / ← truB	30S ribosomal subunit protein S15/tRNA pseudouridine synthase B: tRNA pseudouridine(55) synthase and putative tmRNA pseudouridine(342) synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,311,736	0	G	A	100.0%	235.8 / NA	62	intergenic (‑52/+97)	rpsO/truB	30S ribosomal subunit protein S15/tRNA pseudouridine synthase B: tRNA pseudouridine(55) synthase and putative tmRNA pseudouridine(342) synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/31);  total (31/31)

CAGTGCTACCTGAACTTCGGTAGAACCGGTGTCGTTTGCGTCACGACCAAACTCAGAAACGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGC‑AGTATTCTACTCGT‑AGCGACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTC  >  NC_000913/3311592‑3311880                                                                                                                                                 |                                                                                                                                                   cagTGCTACCTGAACTTCGGTAGAACCGGTGTCGTTTGCGTCACGACCAAACTCAGAAACGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATccc                                                                                                                                               >  1:224969/1‑150 (MQ=255)     gCTACCTGAACTTCGGTAGAACCGGTGTCGTTTGCGTCACGACCAAACTCAGAAACGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAgtg                                                                                                                                           <  2:359706/150‑1 (MQ=255)      cTACCTGAACTTCGGTAGAACCGGTGTCGTTTGCGTCACGACCAAACTCAGAATCGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAAATCCAAAGTATATAGAATGAAAGGCCGCCGATCTCTAATTCAGCATTCACAgtgt                                                                                                                                          >  2:11092/1‑150 (MQ=255)            gAACTTCGGTAGAACCGGTGTCGTTTGCGTCACGACCAAACTCAGAAACGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTAc                                                                                                                                   >  2:523298/1‑151 (MQ=255)             aaCTTCGGTAGAACCGGTGTCGTTTGCGTCACGACCAAACTCAGAAACGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACg                                                                                                                                  <  1:477491/151‑1 (MQ=255)               cTTCGGTAGAACCGGTGTCGTTTGCGTCACGACCAAACTCAGAAACGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGc                                                                                                                                 >  2:25764/1‑150 (MQ=255)                  cGGTAGAACCGGTGTCGTTTGCGTCACGACCAAACTCAGAAACGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCaa                                                                                                                               >  2:635827/1‑149 (MQ=255)                    gTAGAACCGGTGTCGTTTGCGTCACGACCAAACTCAGAAACGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAgtg                                                                                                                           <  1:625639/151‑1 (MQ=255)                     tAGAACCGGTGTCGTTTGCGTCACGACCAAACTCAGAAACGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAgtgt                                                                                                                          >  1:592681/1‑151 (MQ=255)                     tAGAACCGGTGTCGTTTGCGTCACGACCAAACTCAGAAACGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAgtgt                                                                                                                          >  1:461184/1‑151 (MQ=255)                      aGAACCGGTGTCGTTTGCGTCACGACCAAACTCAGAAACGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAgtgt                                                                                                                          <  2:116214/150‑1 (MQ=255)                           cGGTGTCGTTTGCGTCACGACCAAACTCAGAAACGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAAc                                                                                                                     >  1:128804/1‑150 (MQ=255)                           cGGTGTCGTTTGCGTCACGACCAAACTCAGAAACGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAAc                                                                                                                     >  1:472600/1‑150 (MQ=255)                                 cGTTTGCGTCACGACCAAACTCAGAAACGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTAc                                                                                                              >  2:195227/1‑151 (MQ=255)                                 cGTTTGCGTCACGACCAAACTCAGAAACGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTAc                                                                                                              <  1:816656/151‑1 (MQ=255)                                    ttGCGTCACGACCAAACTCAGAAACGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTAcgc                                                                                                            <  1:264788/150‑1 (MQ=255)                                       cGTCACGACCAAACTCAGAAACGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACg                                                                                                        <  1:20470/151‑1 (MQ=255)                                        gTCACGACCAAACTCAGAAACGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGt                                                                                                       <  2:280314/151‑1 (MQ=255)                                         tCACGACCAAACTCAGAAACGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGtt                                                                                                      >  2:217013/1‑151 (MQ=255)                                          cACGACCAAACTCAGAAACGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTATTTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGtt                                                                                                      <  1:771414/150‑1 (MQ=255)                                               ccAAACTCAGAAACGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCgg                                                                                                <  1:453599/151‑1 (MQ=255)                                                cAAACTCAGAAACGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGc                                                                                               >  1:128558/1‑151 (MQ=255)                                                         aaaCGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGC‑AGTATTCt                                                                                      >  2:36055/1‑150 (MQ=255)                                                         aaaCGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGC‑AGTATTCTa                                                                                     <  2:816403/151‑1 (MQ=255)                                                          aaCGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGC‑AGTATTCTAc                                                                                    >  2:888970/1‑151 (MQ=255)                                                             gATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGC‑AGTATTCTACTCg                                                                                 <  2:798682/151‑1 (MQ=255)                                                             gATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGC‑AGTATTCTACTCg                                                                                 >  2:312009/1‑151 (MQ=255)                                                              aTTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGC‑AGTATTCTACTCGt                                                                                >  1:484859/1‑151 (MQ=255)                                                                  tAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGC‑AGTATTCTACTCGT‑AGc                                                                            <  2:621037/150‑1 (MQ=255)                                                                         tGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGC‑AGTATTCTACTCGT‑AGCGACCTGtt                                                                    >  1:903942/1‑151 (MQ=255)                                                                         tGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGC‑AGTATTCTACTCGT‑AGCGACCTGtt                                                                    >  2:522954/1‑151 (MQ=255)                                                                             tCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGC‑AGTATTCTACTCGT‑AGCGACCTGTTATc                                                                 <  1:800777/150‑1 (MQ=255)                                                                              cAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGC‑AGTATTCTACTCGT‑AGCGACCTGTTATCg                                                                <  2:541080/150‑1 (MQ=255)                                                                                  aCTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGC‑AGTATTCTACTCGT‑AGCGACCTGTTATCGCa                                                              >  1:310228/1‑148 (MQ=255)                                                                                  aCTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGC‑AGTATTCTACTCGT‑AGCGACCTGTTATCGCAAg                                                            <  2:407029/150‑1 (MQ=255)                                                                                  aCTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGC‑AGTATTCTACTCGT‑AGCGACCTGTTATCGCAAGa                                                           <  1:725108/151‑1 (MQ=255)                                                                                       gagaCATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGC‑AGTATTCTACTCGT‑AGCGACCTGTTATCGCAAGACGGtt                                                      <  1:425225/151‑1 (MQ=255)                                                                                         gaCATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGC‑AGTATTCTACTCGT‑AGCGACCTGTTATCGCAAGACGGTTaa                                                    <  2:627942/151‑1 (MQ=255)                                                                                          aCATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGC‑AGTATTCTACTCGT‑AGCGACCTGTTATCGCAAGACGGTTaa                                                    <  2:51261/150‑1 (MQ=255)                                                                                           cATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGC‑AGTATTCTACTCGT‑AGCGACCTGTTATCGCAAGACGGTTAACa                                                  <  1:219768/151‑1 (MQ=255)                                                                                            aTTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGC‑AGTATTCTACTCGT‑AGCGACCTGTTATCGCAAGACGGTTAACAt                                                 >  1:741339/1‑151 (MQ=255)                                                                                             ttttAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGC‑AGTATTCTACTCGT‑AGCGACCTGTTATCGCAAGACGGTTAACAtt                                                >  2:624142/1‑151 (MQ=255)                                                                                               ttAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGC‑AGTATTCTACTCGT‑AGCGACCTGTTATCGCAAGACGGTTAACATTa                                               <  1:686573/150‑1 (MQ=255)                                                                                                      tCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGC‑AGTATTCTACTCGT‑AGCGACCTGTTATCGCAAGACGGTTAACATTACGCCGGGt                                       >  1:231532/1‑151 (MQ=255)                                                                                                       ccAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGC‑AGTATTCTACTCGT‑AGCGACCTGTTATCGCAAGACGGTTAACATTACGCCggg                                        <  1:8375/149‑1 (MQ=255)                                                                                                         aaaGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGC‑AGTATTCTACTCGT‑AGCGACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTAtt                                    >  2:325111/1‑151 (MQ=255)                                                                                                          aaGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGC‑AGTATTCTACTCGT‑AGCGACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTc                                   >  2:79944/1‑151 (MQ=255)                                                                                                           aGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGC‑AGTATTCTACTCGT‑AGCGACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCa                                  <  2:762304/151‑1 (MQ=255)                                                                                                              atataGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGC‑AGTATTCTACTCGT‑AGCGACCTGTTATCGCAAGACGGTTAACATTCCGCCGGGTATTCAac                                <  2:842530/150‑1 (MQ=255)                                                                                                               tataGAATGAAAGGACGCTGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGC‑AGTATTCTACTCGT‑AGCGACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAacc                               >  2:103054/1‑150 (MQ=255)                                                                                                                ataGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTAGGGGCGGTTAA‑CGGCAAGAATTCT‑CTTGTAAGCGCCCTGTTTCGGCAAG‑CGGTTAAAAATTAGCCCGGTATTCaaaccac                            >  2:237653/1‑146 (MQ=255)                                                                                                                    aaTGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGC‑AGTATTCTACTCGT‑AGCGACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAgg                         >  2:37373/1‑151 (MQ=255)                                                                                                                     aTGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGC‑AGTATTCTACTCGT‑AGCGACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGc                        <  2:737582/151‑1 (MQ=255)                                                                                                                       gAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGC‑AGTATTCTACTCGT‑AGCGACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGcg                       >  1:492217/1‑150 (MQ=255)                                                                                                                             aCGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGC‑AGTATTCTACTCGT‑AGCGACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGgc                <  2:875653/151‑1 (MQ=255)                                                                                                                               gCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGC‑AGTATTCTACTCGT‑AGCGACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGgcgc              >  2:349318/1‑151 (MQ=255)                                                                                                                                       ctAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGC‑AGTATTCTACTCGT‑AGCGACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAAcgcg        <  2:194723/149‑1 (MQ=255)                                                                                                                                       ctAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGC‑AGTATTCTACTCGT‑AGCGACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAAcg          <  1:239696/147‑1 (MQ=255)                                                                                                                                       ctAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGC‑AGTATTCTACTCGT‑AGCGACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGc      >  2:509223/1‑151 (MQ=255)                                                                                                                                         aaTTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGC‑AGTATTCTACTCGT‑AGCGACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGcc     <  2:48646/150‑1 (MQ=255)                                                                                                                                         aaTTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGC‑AGTATTCTACTCGT‑AGCGACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCt    >  1:675072/1‑151 (MQ=255)                                                                                                                                            tCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGC‑AGTATTCTACTCGT‑AGCGACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTc  <  1:523348/150‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                   CAGTGCTACCTGAACTTCGGTAGAACCGGTGTCGTTTGCGTCACGACCAAACTCAGAAACGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGC‑AGTATTCTACTCGT‑AGCGACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTC  >  NC_000913/3311592‑3311880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑