Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,359,379	T→C	V61A (GTA→GCA)	gltD →	glutamate synthase, 4Fe‑4S protein, small subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,359,379	0	T	C	100.0%	171.5 / NA	48	V61A (GTA→GCA)	gltD	glutamate synthase, 4Fe‑4S protein, small subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/18);  total (30/18)

AGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGAGTTTGTTGAAATTTACGAGCCGTTTTCCGAAGGCCAGGCCAAAGCGCAGGCTGACCGCTGCCTGTCGTGCGGCAACCCATACTGCGAGTGGAAATGCCCGGTACACAACTACATCCCGAACTGGCTGAAGCTCGCCAACGAGGGGCGTATTTTTGAAGCGGCGGAACTGTCGCACCAGACCAACACCCTGCCGGAAGTTTGCGGACGAGTCTGCCCGCAAGACCGTC  >  NC_000913/3359232‑3359504                                                                                                                                                    |                                                                                                                              aGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGAGTTTGTTGAAATTTACGAGCCGTTTTCCGAAGGCCAGGCCAAAGCGCAGGCTGACCGCTGCCTGTCGTGCGGCAACCCATACTGCGAGTGGAAATGCCCGGCac                                                                                                                             >  1:514399/1‑150 (MQ=255)   cgcgTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGAGTTTGTTGAAATTTACGAGCCGTTTTCCGAAGGCCAGGCCAAAGCGCAGGCTGACCGCTGCCTGTCGTGCGGCAACCCATACTGCGAGTGGAAATGCCCGGCacac                                                                                                                           >  2:802639/1‑150 (MQ=255)    gcgTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGAGTTTGTTGAAATTTACGAGCCGTTTTCCGAAGGCCAGGCCAAAGCGCAGGCTGACCGCTGCCTGTCGTGCGGCAACCCATACTGCGAGTGGAAATGCCCGGcacaca                                                                                                                          <  2:795106/150‑1 (MQ=255)    gcgTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGAGTTTGTTGAAATTTACGAGCCGTTTTCCGAAGGCCAGGCCAAAGCGCAGGCTGACCGCTGCCTGTCGTGCGGCAACCCATACTGCGAGTGGAAATGCCCGGcacaca                                                                                                                          >  2:435051/1‑150 (MQ=255)         gATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGAGTTTGTTGAAATTTACGAGCCGTTTTCCGAAGGCCAGGCCAAAGCGCAGGCTGACCGCTGCCTGTCGTGCGGCAACCCATACTGCGAGTGGAAATGCCCGGCACACAACTAc                                                                                                                     <  2:653084/150‑1 (MQ=255)             cGCCAAAGAAACCGCTGAAGATCCGCAAAATTGAGTTTGTTGAAATTTACGAGCCGTTTTCCGAAGGCCAGGCCAAAGCGCAGGCTGACCGCTGCCTGTCGTGCGGCAACCCATACTGCGAGTGGAAATGCCCGGCACACAACTACATccc                                                                                                                >  2:788718/1‑151 (MQ=255)                   aGAAACCGCTGAAGATCCGCAAAATTGAGTTTGTTGAAATTTACGAGCCGTTTTCCGAAGGCCAGGCCAAAGCGCAGGCTGACCGCTGCCTGTCGTGCGGCAACCCATACTGCGAGTGGAAATGCCCGGCACACAACTACATCCCGAACTg                                                                                                          >  1:469582/1‑151 (MQ=255)                        ccGCTGAAGATCCGCAAAATTGAGTTTGTTGAAATTTACGAGCCGTTTTCCGAAGGCCAGGCCAAAGCGCAGGCTGACCGCTGCCTGTCGTGCGGCAACCCATACTGCGAGTGGAAATGCCCGGCACACAACTACATCCCGAACTGGCTg                                                                                                      >  1:301719/1‑150 (MQ=255)                        ccGCTGAAGATCCGCAAAATTGAGTTTGTTGAAATTTACGAGCCGTTTTCCGAAGGCCAGGCCAAAGCGCAGGCTGACCGCTGCCTGTCGTGCGGCAACCCATACTGCGAGTGGAAATGCCCGGCACACAACTACATCCCGAACTGGCTGa                                                                                                     <  1:329470/151‑1 (MQ=255)                              aaGATCCGCAAAATTGAGTTTGTTGAAATTTACGAGCCGTTTTCCGAAGGCCAGGCCAAAGCGCAGGCTGACCGCTGCCTGTCGTGCGGCAACCCATACTGCGAGTGGAAATGCCCGGCACACAACTACATCCCGAACTGGCTGAAGCTc                                                                                                <  2:765841/150‑1 (MQ=255)                              aaGATCCGCAAAATTGAGTTTGTTGAAATTTACGAGCCGTTTTCCGAAGGCCAGGCCAAAGCGCAGGCTGACCGCTGCCTGTCGTGCGGCAACCCATACTGCGAGTGGAAATGCCCGGCACACAACTACATCCCGAACTGGCTGAAGCTc                                                                                                <  2:612744/150‑1 (MQ=255)                              aaGATCCGCAAAATTGAGTTTGTTGAAATTTACGAGCCGTTTTCCGAAGGCCAGGCCAAAGCGCAGGCTGACCGCTGCCTGTCGTGCGGCAACCCATACTGCGAGTGGAAATGCCCGGCACACAACTACATCCCGAACTGGCTGAAGCTCg                                                                                               >  2:132609/1‑151 (MQ=255)                              aaGATCCGCAAAATTGAGTTTGTTGAAATTTACGAGCCGTTTTCCGAAGGCCAGGCCAAAGCGCAGGCTGACCGCTGCCTGTCGTGCGGCAACCCATACTGCGAGTGGAAATGCCCGGCACACAACTACATCCCGAACTGGCTGAAGCTCg                                                                                               >  1:337902/1‑151 (MQ=255)                                    cGCAAAATTGAGTTTGTTGAAATTTACGAGCCGTTTTCCGAAGGCCAGGCCAAAGCGCAGGCTGACCGCTGCCTGTCGTGCGGCAACCCATACTGCGAGTGGAAATGCCCGGCACACAACTACATCCCGAACTGGCTGAAGCTCGCCAACg                                                                                         >  1:395227/1‑151 (MQ=255)                                    cGCAAAATTGAGTTTGTTGAAATTTACGAGCCGTTTTCCGAAGGCCAGGCCAAAGCGCAGGCTGACCGCTGCCTGTCGTGCGGCAACCCATACTGCGAGTGGAAATGCCCGGCACACAACTACATCCCGAACTGGCTGAAGCTCGCCAACg                                                                                         >  1:589036/1‑151 (MQ=255)                                      cAAAATTGAGTTTGTTGAAATTTACGAGCCGTTTTCCGAAGGCCAGGCCAAAGCCCAGGCTGACCGCTGCCTGTCGTGCGGCAACCCATACTGCGAGTGGAAATGCCCGGCACTCAACTACATCCCGAACTGGCTGAAGCCCGCCAACg                                                                                         >  2:864162/1‑149 (MQ=255)                                        aaaTTGAGTTTGTTGAAATTTACGAGCCGTTTTCCGAAGGCCAGGCCAAAGCGCAGGCTGACCGCTGCCTGTCGTGCGGCAACCCATACTGCGAGTGGAAATGCCCGGCACACAACTACATCCCGAACTGGCTGAAGCTCGCCAACGAggg                                                                                     >  1:161458/1‑151 (MQ=255)                                          aTTGAGTTTGTTGAAATTTACGAGCCGTTTTCCGAAGGCCAGGCCAAAGCGCAGGCTGACCGCTGCCTGTCGTGCGGCAACCCATACTGCGAGTGGAAATGCCCGGCACACAACTACATCCCGAACTGGCTGAAGCTCGCCAACGAGGGGc                                                                                   >  2:152119/1‑151 (MQ=255)                                             gAGTTTGTTGAAATTTACGAGCCGTTTTCCGAAGGCCAGGCCAAAGCGCAGGCTGACCGCTGCCTGTCGTGCGGCAACCCATACTGCGAGTGGAAATGCCCGGCACACAACTACATCCCGAACTGGCTGAAGCTCGCCAACGAGGGGCGt                                                                                 <  1:385348/150‑1 (MQ=255)                                                  tgttgAAATTTACGAGCCGTTTTCCGAAGGCCAGGCCAAAGCGCAGGCTGACCGCTGCCTGTCGTGCGGCAACCCATACTGCGAGTGGAAATGCCCGGCACACAACTACATCCCGAACTGGCTGAAGCTCGCCAACGAGGGGCGTAttttt                                                                           >  2:639947/1‑151 (MQ=255)                                                   gttgAAATTTACGAGCCGTTTTCCGAAGGCCAGGCCAAAGCGCAGGCTGACCGCTGCCTGTCGTGCGGCAACCCATACTGCGAGTGGAAATGCCCGGCATACAACTACATCCCGAACTGGCTGAAGCTCGCCAACGAGGGGCGTATTTTTg                                                                          >  1:444255/1‑151 (MQ=255)                                                   gttgAAATTTACGAGCCGTTTTCCGAAGGCCAGGCCAAAGCGCAGGCTGACCGCTGCCTGTCGTGCGGCAACCCATACTGCGAGTGGAAATGCCCGGCACACAACTACATCCCGAACTGGCTGAAGCTCGCCAACGAGGGGCGTATTTTTg                                                                          >  2:557751/1‑151 (MQ=255)                                                      gAAATTTACGAGCCGTTTTCCGAAGGCCAGGCCAAAGCGCAGGCTGACCGCTGCCTGTCGTGCGGCAACCCATACTGCGAGTGGAAATGCCCGGCACACAACTACATCCCGAACTGGCTGAAGCTCGCCAACGAGGGGCGTATTTTTGa                                                                         >  2:608573/1‑149 (MQ=255)                                                       aaaTTTACGAGCCGTTTTCCGAAGGCCAGGCCAAAGCGCAGGCTGACCGCTGCCTGTCGTGCGGCAACCCATACTGCGAGTGGAAATGCCCGGCACACAACTACATCCCGAACTGGCTGAAGCTCGCCAACGAGGGGCGTATTTTTGAAgc                                                                      <  2:588987/151‑1 (MQ=255)                                                       aaaTTTACGAGCCGTTTTCCGAAGGCCAGGCCAAAGCGCAGGCTGACCGCTGCCTGTCGTGCGGCAACCCATACTGCGAGTGGAAATGCCCGGCACACAACTACATCCCGAACTGGCTGAAGCTCGCCAACGAGGGGCGTATTTTTGAAgc                                                                      <  1:814459/151‑1 (MQ=255)                                                           ttACGAGCCGTTTTCCGAAGGCCAGGCCAAAGCGCAGGCTGACCGCTGCCTGTCGTGCGGCAACCCATACTGCGAGTGGAAATGCCCGGCACACAACTACATCCCGAACTGGCTGAAGCTCGCCAACGAGGGGCGTATTTTTGAAgcggcg                                                                  >  1:406630/1‑151 (MQ=255)                                                            tACGAGCCGTTTTCCGAAGGCCAGGCCAAAGCGCAGGCTGACCGCTGCCTGTCGTGCGGCAACCCATACTGCGAGTGGAAATGCCCGGCACACAACTACATCCCGAACTGGCTGAAGCTCGCCAACGAGGGGCGTATTTTTGAAgcggcg                                                                  <  1:132631/150‑1 (MQ=255)                                                              cGAGCCGTTTTCCGAAGGCCAGGCCAAAGCGCAGGCTGACCGCTGCCTGTCGTGCGGCAACCCATACTGCGAGTGGAAATGCCCGGCACACAACTACATCCCGAACTGGCTGAAGCTCGCCAACGAGGGGCGTATTTTTGAAGCGGCGGa                                                                >  1:666294/1‑150 (MQ=255)                                                               gAGCCGTTTTCCGAAGGCCAGGCCAAAGCGCAGGCTGACCGCTGCCTGTCGTGCGGCAACCCATACTGCGAGTGGAAATGCCCGGCACACAACTACATCCCGAACTGGCTGAAGCTCGCCAACGAGGGGCGTATTTTTGAAGCGGCGGAAc                                                              >  2:55058/1‑151 (MQ=255)                                                                   cGTTTTCCGAAGGCCAGGCCAAAGCGCAGGCTGACCGCTGCCTGTCGTGCGGCAACCCATACTGCGAGTGGAAATGCCCGGCACACAACTACATCCCGAACTGGCTGAAGCTCGCCAACGAGGGGCGTATTTTTGAAGCGGCGGAACTGTc                                                          >  1:195379/1‑151 (MQ=255)                                                                   cGTTTTCCGAAGGCCAGGCCAAAGCGCAGGCTGACCGCTGCCTGTCGTGCGGCAACCCATACTGCGAGTGGAAATGCCCGGCACACAACTACATCCCGAACTGGCTGAAGCTCGCCAACGAGGGGCGTATTTTTGAAGCGGCGGAACTGTc                                                          <  1:60405/151‑1 (MQ=255)                                                                       ttCCGAAGGCCAGGCCAAAGCGCAGGCTGACCGCTGCCTGTCGTGCGGCAACCCATACTGCGAGTGGAAATGCCCGGCACACAACTACATCCCGAACTGGCTGAAGCTCGCCAACGAGGGGCGTATTTTTGAAGCGGCGGAACTGTCGCa                                                       <  2:337876/150‑1 (MQ=255)                                                                       ttCCGAAGGCCAGGCCAAAGCGCAGGCTGACCGCTGCCTGTCGTGCGGCAACCCATACTGCGAGTGGAAATGCCCGGCACACAACTACATCCCGAACTGGCTGAAGCTCGCCAACGAGGGGCGTATTTTTGAAGCGGCGGAACTGTCGCAc                                                      >  1:801340/1‑151 (MQ=255)                                                                         ccGAAGGCCAGGCCAAAGCGCAGGCTGACCGCTGCCTGTCGTGCGGCAACCCATACTGCGAGTGGAAATGCCCGGCACACAACTACATCCCGAACTGGCTGAAGCTCGCCAACGAGGGGCGTATTTTTGAAGCGGCGGAACTGTCGCAcc                                                     >  1:758234/1‑150 (MQ=255)                                                                          cGAAGGCCAGGCCAAAGCGCAGGCTGACCGCTGCCTGTCGTGCGGCAACCCATACTGCGAGTGGAAATGCCCGGCACACAACTACATCCCGAACTGGCTGAAGCTCGCCAACGAGGGGCGTATTTTTGAAGCGGCGGAACTGTCGCAcc                                                     >  2:223062/1‑149 (MQ=255)                                                                              ggccaggccaAAGCGCAGGCTGACCGCTGCCTGTCGTGCGGCAACCCATACTGCGAGTGGAAATGCCCGGCACACAACTACATCCCGAACTGGCTGAAGCTCGCCAACGAGGGGCGTATTTTTGAAGCGGCGGAACTGTCGCACCAGACCa                                               <  1:559208/151‑1 (MQ=255)                                                                               gccaggccaAAGCGCAGGCTGACCGCTGCCTGTCGTGCGGCAACCCATACTGCGAGTGGAAATGCCCGGCACACAACTACATCCCGAACTGGCTGAAGCTCGCCAACGAGGGGCGTATTTTTGAAGCGGCGGAACTGTCGCACCAGAc                                                 >  1:344228/1‑148 (MQ=255)                                                                                 caggccaAAGCGCAGGCTGACCGCTGCCTGTCGTGCGGCAACCCATACTGCGAGTGGAAATGCCCGGCACACAACTACATCCCGAACTGGCTGAAGCTCGCCAACGAGGGGCGTATTTTTGAAGCGGCGGAACTGTCGCACCAGACCAaca                                            <  2:512105/151‑1 (MQ=255)                                                                                         aGCGCAGGCTGACCGCTGCCTGTCGTGCGGCAACCCATACTGCGAGTGGAAATGCCCGGCACACAACTACATCCCGAACTGGCTGAAGCTCGCCAACGAGGGGCGTATTTTTGAAGCGGCGGAACTGTCGCACCAGACCAACACCCTGCCg                                    <  2:719461/151‑1 (MQ=255)                                                                                          gcgcAGGCTGACCGCTGCCTGTCGTGCGGCAACCCATACTGCGAGTGGAAATGCCCGGCACACAACTACATCCCGAACTGGCTGAAGCTCGCCAACGAGGGGCGTATTTTTGAAGCGGCGGAACTGTCGCACCAGACCAACACCCTGCCgg                                   <  2:765078/151‑1 (MQ=255)                                                                                                   gACCGCTGCCTGTCGTGCGGCAACCCATACTGCGAGTGGAAATGCCCGGCACACAACTACATCCCGAACTGGCTGAAGCTCGCCAACGAGGGGCGTATTTTTGAAGCGGCGGAACTGTCGCACCAGACCAACACCCTGCCGGAAGTTTg                            <  1:639996/149‑1 (MQ=255)                                                                                                     ccGCTGCCTGTCGTGCGGCAACCCATACTGCGAGTGGAAATGCCCGGCACACAACTACATCCCGAACTGGCTGAAGCTCGCCAACGAGGGGCGTATTTTTGAAGCGGCGGAACTGTCGCACCAGACCAACACCCTGCCGGAAGTTTGCGGa                        <  1:244703/151‑1 (MQ=255)                                                                                                            cTGTCGTGCGGCAACCCATACTGCGAGTGGAAATGCCCGGCACACAACTACATCCCGAACTGGCTGAAGCTCGCCAACGAGGGGCGTATTTTTGAAGCGGCGGAACTGTCGCACCAGACCAACACCCTGCCGGAAGTTTGCGGACGAGTCt                 >  1:387681/1‑151 (MQ=255)                                                                                                            cTGTCGTGCGGCAACCCATACTGCGAGTGGAAATGCCCGGCACACAACTACATCCCGAACTGGCTGAAGCTCGCCAACGAGGGGCGTATTTTTGAAGCGGCGGAACTGTCGCACCAGACCAACACCCTGCCGGAAGTTTGCGGACGAGTCt                 >  2:802524/1‑151 (MQ=255)                                                                                                             tGTCGTGCGGCAACCCATACTGCGAGTGGAAATGCCCGGCACACAACTACATCCCGAACTGGCTGAAGCTCGCCAACGAGGGGCGTATTTTTGAAGCGGCGGAACTGTCGCACCAGACCAACACCCTGCCGGAAGTTTGCGGACGAGTCTg                >  1:45156/1‑151 (MQ=255)                                                                                                              gTCGTGCGGCAACCCATACTGCGAGTGGAAATGCCCGGCACACAACTACATCCCGAACTGGCTGAAGCTCGCCAACGAGGGGCGTATTTTTGAAGCGGCGGAACTGTCGCACCAGACCAACACCCTGCCGGAAGTTTGCGGACGAGTCt                 <  1:523334/149‑1 (MQ=255)                                                                                                                 gTGCGGCAACCCATACTGCGAGTGGAAATGCCCGGCATACAACTACATCCCGAACTGGCTGAAGCTCGCCAACGAGGGGCGTATTTTTGAAGCGGCGGAACTGTCGCACCAGACCAACACCCTGCCGGAAGTTTGCGGACGAGTCTGCCCg            <  2:444208/151‑1 (MQ=255)                                                                                                                       cAACCCATACTGCGAGTGGAAATGCCCGGCACACAACTACATCCCGAACTGGCTGAAGCTCGCCAACGAGGGGCGTATTTTTGAAGCGGCGGAACTGTCGCACCAGACCAACACCCTGCCGGAAGTTTGCGGACGAGTCTGCCCGCAAGAc      >  1:452755/1‑151 (MQ=255)                                                                                                                        aaCCCATACTGCGAGTGGAAATGCCCGGCACACAACTACATCCCGAACTGGCTGAAGCTCGCCAACGAGGGGCGTATTTTTGAAGCGGCGGAACTGTCGCACCAGACCAACACCCTGCCGGAAGTTTGCGGACGAGTCTGCCCGCAAGAcc     >  1:280297/1‑151 (MQ=255)                                                                                                                           ccATACTGCGAGTGGAAATGCCCGGCACACAACTACATCCCGAACTGGCTGAAGCTCGCCAACGAGGGGCGTATTTTTGAAGCGGCGGAACTGTCGCACCAGACCAACACCCTGCCGGAAGTTTGCGGACGAGTCTGCCCGCAAGACCGTc  >  1:348032/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                              AGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGAGTTTGTTGAAATTTACGAGCCGTTTTCCGAAGGCCAGGCCAAAGCGCAGGCTGACCGCTGCCTGTCGTGCGGCAACCCATACTGCGAGTGGAAATGCCCGGTACACAACTACATCCCGAACTGGCTGAAGCTCGCCAACGAGGGGCGTATTTTTGAAGCGGCGGAACTGTCGCACCAGACCAACACCCTGCCGGAAGTTTGCGGACGAGTCTGCCCGCAAGACCGTC  >  NC_000913/3359232‑3359504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑