Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,416,884	G→A	G618S (GGC→AGC)	acrF →	multidrug efflux system protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,416,884	0	G	A	100.0%	247.8 / NA	65	G618S (GGC→AGC)	acrF	multidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/30);  total (35/30)

GGTGTCTTTCTGACCATGATTCAGTTACCCGCTGGCGCGACGCAAGAGCGGACGCAAAAAGTGTTGGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCGGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCGTGCCAAAATGGAATTGGGCAAGATCCGCGACGGTTTTGTCATTCCATT  >  NC_000913/3416737‑3417026                                                                                                                                                    |                                                                                                                                               ggTGTCTTTCTGACCATGATTCAGTTACCCGCTGGCGCGACGCAAGAGCGGACGCAAAAAGTGTTGGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCAGcc                                                                                                                                             >  2:588263/1‑151 (MQ=255)  gtgtCTTTCTGACCATGATTCAGTTACCCGCTGGCGCGACGCAAGAGCGGACGCAAAAAGTGTTGGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCAGCCa                                                                                                                                            <  1:306862/151‑1 (MQ=255)   tgtCTTTCTGACCATGATTCAGTTACCCGCTGGCGCGACGCAAGAGCGGACGCAAAAAGTGTTGGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCAGCCa                                                                                                                                            <  1:54609/150‑1 (MQ=255)   tgtCTTTCTGACCATGATTCAGTTACCCGCTGGCGCGACGCAAGAGCGGACGCAAAAAGTGTTGGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCAGCCa                                                                                                                                            <  1:283690/150‑1 (MQ=255)          cTGACCATGATTCAGTTACCCGCTGGCGCGACGCAAGAGCGGACGCAAAAAGTGTTGGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACaa                                                                                                                                     >  2:807674/1‑150 (MQ=255)            gACCATGATTCAGTTACCCGCTGGCGCGACGCAAGAGCGGACGCAAAAAGTGTTGGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAAc                                                                                                                                  <  2:85921/151‑1 (MQ=255)               cATGATTCAGTTACCCGCTGGCGCGACGCAAGAGCGGACGCAAAAAGTGTTGGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGcc                                                                                                                               >  2:159619/1‑151 (MQ=255)                 tGATTCAGTTACCCGCTGGCGCGACGCAAGAGCGGACGCAAAAAGTGTTGGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCgg                                                                                                                             >  2:296482/1‑151 (MQ=255)                          tACCCGCTGGCGCGACGCAAGAGCGGACGCAAAAAGTGTTGGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCt                                                                                                                     <  2:731262/150‑1 (MQ=255)                           aCCCGCTGGCGCGACGCAAGAGCGGACGCAAAAAGTGTTGGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTc                                                                                                                   <  1:119501/151‑1 (MQ=255)                              cGCTGGCGCGACGCAAGAGCGGACGCAAAAAGTGTTGGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTCGTc                                                                                                                <  2:819921/151‑1 (MQ=255)                               gCTGGCGCGACGCAAGAGCGGACGCAAAAAGTGTTGGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTCGTc                                                                                                                >  2:692689/1‑150 (MQ=255)                                 tGGCGCGACGCAAGAGCGGACGCAAAAAGTGTTGGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGt                                                                                                             >  2:135564/1‑151 (MQ=255)                                 tGGCGCGACGCAAGAGCGGACGCAAAAAGTGTTGGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGt                                                                                                             >  1:46024/1‑151 (MQ=255)                                   gcgcGACGCAAGAGCGGACGCAAAAAGTGTTGGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCt                                                                                                           <  1:834646/151‑1 (MQ=255)                                   gcgcGACGCAAGAGCGGACGCAAAAAGTGTTGGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCt                                                                                                           <  1:469510/151‑1 (MQ=255)                                    cgcgACGCAAGAGCGGACGCAAAAAGTGTTGGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTg                                                                                                          >  1:195304/1‑151 (MQ=255)                                       gACGCAAGAGCGGACGCAAAAAGTGTTGGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGaa                                                                                                        >  2:606604/1‑150 (MQ=255)                                            aaGAGCGGACGCAAAAAGTGTTGGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGACCGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAAcc                                                                                                     >  2:615875/1‑148 (MQ=255)                                              gagCGGACGCAAAAAGTGTTGGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTggg                                                                                                >  1:868911/1‑151 (MQ=255)                                               agCGGACGCAAACAGTGTTGGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTggg                                                                                                >  2:455875/1‑150 (MQ=255)                                                 cGGACGCAAAAAGTGTTGGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTGGGa                                                                                               >  1:95954/1‑149 (MQ=255)                                                  ggACGCAAAAAGTGTTGGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTGGGa                                                                                               >  1:206502/1‑148 (MQ=255)                                                  ggACGCAAAAAGTGTTGGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTGGGAaga                                                                                            <  1:509551/151‑1 (MQ=255)                                                  ggACGCAAAAAGTGTTGGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTGGGAaga                                                                                            <  2:422922/151‑1 (MQ=255)                                                     cGCAAAAAGTGTTGGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTGGGAAGAGCg                                                                                         <  2:559502/151‑1 (MQ=255)                                                     cGCAAAAAGTGTTGGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTGGGAAGAGCg                                                                                         >  1:628720/1‑151 (MQ=255)                                                      gCAAAAAGTGTTGGATCAAGATACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTGGGAAGAGCg                                                                                         >  2:483603/1‑150 (MQ=255)                                                          aaaGTGTTGGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTGGGAAGAGCGTAATg                                                                                    <  1:206513/151‑1 (MQ=255)                                                           aaGTGTTGGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTGGGAAGAGCGTAATgg                                                                                   >  1:6494/1‑151 (MQ=255)                                                            aGTGTTGGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTGGGAAGAGCGTAATGGt                                                                                  <  2:312088/151‑1 (MQ=255)                                                                      cAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTGGGAAGAGCGTAATGGTGACGAAAAc                                                                         >  1:395254/1‑150 (MQ=255)                                                                      cAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTGGGAAGAGCGTAATGGTGACGAAAACa                                                                        <  2:349871/151‑1 (MQ=255)                                                                            aaggATTAATGCCTGAAGAACGCGAAAGAGAACGTTGAAAGAGGCTTTACGGTTAACGACTTTAGCTTCAACAGCAAAGCACAACACGCCGGTATGGCCTTCGTCAGTCTGTAACAGTGGGAAGAGCGTATTGGTGACGAAAACAGTGc                                                                    >  2:101562/3‑149 (MQ=255)                                                                              ggATTGATATCTGAAGAACGAGAAAGCGAACATTGAAAGTGTCTTCACGGTTACCTGCTTTAGCTTCAGCAGCCAGGCACAAAACGACGGTATGGCCTTCGTCAGTCAGAAACCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGCaa                                                                <  2:475802/151‑1 (MQ=255)                                                                                aTTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGc                                                              <  1:263941/151‑1 (MQ=255)                                                                                  tACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCg                                                             >  2:73500/1‑150 (MQ=255)                                                                                  tACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCg                                                             <  1:73522/150‑1 (MQ=255)                                                                                   aCTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGt                                                           <  2:558454/151‑1 (MQ=255)                                                                                   aCTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGt                                                           >  1:898020/1‑151 (MQ=255)                                                                                          gaagaaCGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCa                                                     <  1:450986/150‑1 (MQ=255)                                                                                                cGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCGTGcc                                              >  1:714691/1‑151 (MQ=255)                                                                                                   gaAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCGTGCCaa                                            >  1:169656/1‑150 (MQ=255)                                                                                                       gCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCGTGCCAAAATgg                                       >  2:854762/1‑151 (MQ=255)                                                                                                         gAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCGTGCCAAAATGGa                                      >  1:770918/1‑150 (MQ=255)                                                                                                          aaCGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCGTGCCAAAATGGAAt                                    >  2:769529/1‑151 (MQ=255)                                                                                                           aCGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCGTGCCAAAATGGAAtt                                   <  1:379990/151‑1 (MQ=255)                                                                                                             gTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCGTGCCAAAATGGAATTgg                                 <  1:489471/151‑1 (MQ=255)                                                                                                             gTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGAGGTAATCCATCGTGCCAAAATGGAATTgg                                 >  1:583587/1‑151 (MQ=255)                                                                                                              ttGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCGTGCCAAAATGGAATTggg                                >  1:490603/1‑151 (MQ=255)                                                                                                                gAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCGTGCCAAAATGGAATTGGGc                               <  1:905650/150‑1 (MQ=255)                                                                                                                   aGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCGTGCCAAAATGGAATTGGGCAAGa                           <  1:882643/151‑1 (MQ=255)                                                                                                                       tCTTTACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCGTGCCAAAATGGAATTGGGCAAGATCcg                       >  2:509709/1‑151 (MQ=255)                                                                                                                          ttACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCGTGCCAAAATGGAATTGGGCAAGATCcgcg                     <  1:820473/150‑1 (MQ=255)                                                                                                                          ttACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCGTGCCAAAATGGAATTGGGCAAGATCcgcg                     >  2:747898/1‑150 (MQ=255)                                                                                                                          ttACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCGTGCCAAAATGGAATTGGGCAAGATCcgc                      <  2:897947/149‑1 (MQ=255)                                                                                                                          ttACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCGTGCCAAAATGGAATTGGGCAAGATCcgc                      <  1:178653/149‑1 (MQ=255)                                                                                                                           tACGGTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCGTGCCAAAATGGAATTGGGCAAGATCCGCGAc                   >  1:623300/1‑151 (MQ=255)                                                                                                                              ggTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCGTGCCAAAATGGAATTGGGCAAGATCCGCGACGGt                >  1:315122/1‑151 (MQ=255)                                                                                                                               gTTAACGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTAGTCAGTCTGAAACCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCGTGCCAAAATGGAATTGGGCAAGATCCGCGACGGtt               >  1:599176/1‑151 (MQ=255)                                                                                                                                ttATCGGCTTTAGCTTCAGAAGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTGGGAAGAGCGTAATGGTGATGACAACAGTGCGGAAGCGGTAATCCATCGAGCCAAATTGGAATTGGGCAAGCTCAGCGACGGgtt              >  2:151890/1‑151 (MQ=255)                                                                                                                                  aaCGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCGTGCCAAAATGGAATTGGGCAAGATCCGCGACGGTTTTg            <  1:544746/151‑1 (MQ=255)                                                                                                                                   aCGGCTTTAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCGTGCCAAAATGGAATTGGGCAAGATCCGCGACGGTTTTGt           <  2:626337/151‑1 (MQ=255)                                                                                                                                        tttAGCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCGTGCCAAAATGGAATTGGGCAAGATCCGCGACGGTTTTGTcattc      >  1:249482/1‑151 (MQ=255)                                                                                                                                            gCTTCAGCAGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCGTGCCAAAATGGAATTGGGCAAGATCCGCGACGGTTTTGTcattccatt  <  1:777337/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                               GGTGTCTTTCTGACCATGATTCAGTTACCCGCTGGCGCGACGCAAGAGCGGACGCAAAAAGTGTTGGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCGGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTGGGAAGAGCGTAATGGTGACGAAAACAGTGCGGAAGCGGTAATCCATCGTGCCAAAATGGAATTGGGCAAGATCCGCGACGGTTTTGTCATTCCATT  >  NC_000913/3416737‑3417026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑