Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	398,614	A→G	N248S (AAC→AGC)	yaiW →	microcin Bac7 uptake protein; outer membrane surface‑exposed lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	398,614	0	A	G	100.0%	159.9 / NA	46	N248S (AAC→AGC)	yaiW	microcin Bac7 uptake protein; outer membrane surface‑exposed lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/26);  total (20/26)

CAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTT  >  NC_000913/398472‑398761                                                                                                                                               |                                                                                                                                                    cAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCa                                                                                                                                             <  1:101239/151‑1 (MQ=255)      aGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAg                                                                                                                                        <  1:334316/151‑1 (MQ=255)         cTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCCCCCGTTATAGCGCACCGATATAACGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGcc                                                                                                                                     >  2:217851/1‑151 (MQ=255)           tCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGcc                                                                                                                                     <  1:530289/149‑1 (MQ=255)            cAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGc                                                                                                                                      <  1:604009/147‑1 (MQ=255)                cgccgcGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAgcggc                                                                                                                               <  2:900187/150‑1 (MQ=255)                cgccgcGGCGGGTTGTGGTTTGGTACTTACCAATTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGGACGCCAGCCGGAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAgcgg                                                                                                                                >  1:566256/1‑149 (MQ=255)                       gcggGTGGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCt                                                                                                                       <  2:274204/151‑1 (MQ=255)                         gggTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTg                                                                                                                      <  1:298632/150‑1 (MQ=255)                                   ttGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACg                                                                                                            <  1:19701/150‑1 (MQ=255)                                    tGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACgg                                                                                                           <  1:104352/150‑1 (MQ=255)                                            aCCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTaa                                                                                                   <  1:94275/150‑1 (MQ=255)                                                    cTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTAc                                                                                           <  1:705802/150‑1 (MQ=255)                                                     tGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTAc                                                                                           <  2:325848/149‑1 (MQ=255)                                                      gAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGAc                                                                                        <  1:147232/151‑1 (MQ=255)                                                        aTTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAg                                                                                      <  1:54084/151‑1 (MQ=255)                                                        aTTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAg                                                                                      <  1:141746/151‑1 (MQ=255)                                                                 ccAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAAcc                                                                             >  2:388085/1‑151 (MQ=255)                                                                   aGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCg                                                                           >  2:894848/1‑151 (MQ=255)                                                                   aGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCg                                                                           >  1:16776/1‑151 (MQ=255)                                                                     ttATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCgg                                                                          <  2:790536/150‑1 (MQ=255)                                                                     ttATAGCGCACCGATATACCGTTGTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCgg                                                                          <  1:101714/150‑1 (MQ=255)                                                                        taGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGaa                                                                       >  2:629233/1‑150 (MQ=255)                                                                              aCCGATATACCGCTTTGCTGATTTTAACGCTGCCTGGCACCCCACCCGCAATGCCGCGTTTCAGAGCGCGGCCAGTAAGGCCAGCGCCGCGAAGCCGGCACTGGACGGCGATTTAATTCGCTACGACAGTAAAGAACCCGGGAAAAcc                                                                   >  2:29692/1‑148 (MQ=255)                                                                                   tataCCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGc                                                           >  1:539469/1‑151 (MQ=255)                                                                                    ataCCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGc                                                           >  1:174502/1‑150 (MQ=255)                                                                                         gTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAAcgcg                                                     <  1:790875/151‑1 (MQ=255)                                                                                           tttGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACgcgc                                                    >  1:857994/1‑150 (MQ=255)                                                                                           tttGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACgcgc                                                    <  2:103581/150‑1 (MQ=255)                                                                                                 gATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTgg                                             >  1:199138/1‑151 (MQ=255)                                                                                                  aTTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGc                                            >  1:865477/1‑151 (MQ=255)                                                                                                     ttAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGc                                         >  1:365897/1‑151 (MQ=255)                                                                                                      tAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGc                                         >  2:844382/1‑150 (MQ=255)                                                                                                       aaCGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCa                                        >  2:598550/1‑150 (MQ=255)                                                                                                         cGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCaaaa                                     <  1:852039/151‑1 (MQ=255)                                                                                                         cGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCaaaa                                     <  1:795437/151‑1 (MQ=255)                                                                                                            tggctggTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTg                                  >  1:228267/1‑151 (MQ=255)                                                                                                             ggctggTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTg                                  <  2:491533/150‑1 (MQ=255)                                                                                                                 ggTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACGGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGa                              <  1:162379/150‑1 (MQ=255)                                                                                                                 ggTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGAt                             <  1:734364/151‑1 (MQ=255)                                                                                                                    aCGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATg                            >  1:300875/1‑149 (MQ=255)                                                                                                                    aCGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATg                            >  1:277388/1‑149 (MQ=255)                                                                                                                         aGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCg                        <  1:507390/148‑1 (MQ=255)                                                                                                                               aaTGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGaa                <  2:259485/150‑1 (MQ=255)                                                                                                                                          tCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTc      >  1:720488/1‑149 (MQ=255)                                                                                                                                           cAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGt   >  1:557322/1‑151 (MQ=255)                                                                                                                                            agagCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGtt  >  2:192925/1‑151 (MQ=255)                                                                                                                                               |                                                                                                                                                    CAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTT  >  NC_000913/398472‑398761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑