Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,514,996	T→C	T22A (ACC→GCC)	rpe ←	D‑ribulose‑5‑phosphate 3‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,514,996	0	T	C	100.0%	194.3 / NA	53	T22A (ACC→GCC)	rpe	D‑ribulose‑5‑phosphate 3‑epimerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/30);  total (23/30)

ATCAGGTGTACGTCGATAGGGGCGGTAATGCCATAGTTACGCAAGGATTTCAGCACCATTGGCCCAATCGTCAGATTGGGAACATAGTGGTTATCCATGACGTCAAAATGCACGACATCAGCGCCAGCTGCCAGGGCTTTTGCGGTATCTTCACCCAGGCGGGCAAAATCAGCCGACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACAAAGCCAGCAGTTCGTCCACCTT  >  NC_000913/3514851‑3515136                                                                                                                                                  |                                                                                                                                             aTCAGGTGTACGTCGATAGGGGCGGTAATGCCCTAGTTACGCAAGGATTTCAGCACCATTGGCCCAATCGTCAGATTGGGAACATAGGGGTTATCCATGACGTCAAAATGCACGACATCAGCGCCAGCTGCCAGGGCTTTTGCGGCATCtt                                                                                                                                         >  2:469323/1‑151 (MQ=255) aTCAGGTGTACGTCGATAGGGGCGGTAATGCCATAGTTACGCAAGGATTTCAGCACCATTGGCCCAATCGTCAGATTGGGAACATAGTGGTTATCCATGACGTCAAAATGCACGACATCAGCGCCAGCTGCCAGGGCTTTTGCGGCATCtt                                                                                                                                         <  2:317257/151‑1 (MQ=255)   cAGGTGTACGTCGATAGGGGCGGTAATGCCATAGTTACGCAAGGATTTCAGCACCATTGGCCCAATCGTCAGATTGGGAACATAGTGGTTATCCATGACGTCAAAATGCACGACATCAGCGCCAGCTGCCAGGGCTTTTGCGGCATCTTCa                                                                                                                                       <  1:682373/151‑1 (MQ=255)    aGGTGTACGTCGATAGGGGCGGTAATGCCATAGTTACGCAAGGATTTCAGCACCATTGGCCCAATCGTCAGATTGGGAACATAGTGGTTATCCATGACGTCAAAATGCACGACATCAGCGCCAGCTGCCAGGGCTTTTGCGGCATCTTCAc                                                                                                                                      <  1:116294/151‑1 (MQ=255)        gtACGTCGATAGGGGCGGTAATGCCATAGTTACGCAAGGATTTCAGCACCATTGGCCCAATCGTCAGATTGGGAACATAGTGGTTATCCATGACGTCAAAATGCACGACATCAGCGCCAGCTGCCAGGGCTTTTGCGGCATCTTCACCCa                                                                                                                                   <  1:362886/150‑1 (MQ=255)               gATAGGGGCGGTAATGCCATAGTTACGCAAGGATTTCAGCACCATTGGCCCAATCGTCAGATTGGGAACATAGTGGTTATCCATGACGTCAAAATGCACGACATCAGCGCCAGCTGCCAGGGCTTTTGCGGCATCTTCACCCAGGCGGGc                                                                                                                            >  2:444495/1‑150 (MQ=255)               gATAGGGGCGGTAATGCCATAGTTACGCAAGGATTTCAGCACCATTGGCCCAATCGTCAGATTGGGAACATAGTGGTTATCCATGACGTCAAAATGCACGACATCAGCGCCAGCTGCCAGGGCTTTTGCGGCATCTTCACCCAGGCGGGCa                                                                                                                           <  2:814476/151‑1 (MQ=255)                      gCGGTAATGCCATAGTTACGCAAGGATTTCAGCACCATTGGCCCAATCGTCAGATTGGGAACATAGTGGTTATCCATGACGTCAAAATGCACGACATCAGCGCCAGCTGCCAGGGCTTTTGCGGCATCTTCACCCAGGCGGGCAAAATc                                                                                                                      >  1:701693/1‑149 (MQ=255)                       cGGTAATGCCATAGTTACGCAAGGATTTCAGCACCATTGGCCCAATCGTCAGATTGGGAACATAGTGGTTATCCATGACGTCAAAATGCACGACATCAGCGCCAGCTGCCAGGGCTTTTGCGGCATCTTCACCCAGGCGGGCAAAATCAGc                                                                                                                   >  1:593963/1‑151 (MQ=255)                            aTGCCATAGTTACGCAAGGATTTCAGCACCATTGGCCCAATCGTCAGATTGGGAACATAGTGGTTATCCATGACGTCAAAATGCACGACATCAGCGCCAGCTGCCAGGGCTTTTGCGGCATCTTCACCCAGGCGGGCAAAATCAGCCGAc                                                                                                               >  2:112681/1‑150 (MQ=255)                                 aTAGTTACGCAAGGATTTCAGCACCATTGGCCCAATCGTCAGATTGGGAACATAGTGGTTATCCATGACGTCAAAATGCACGACATCAGCGCCAGCTGCCAGGGCTTTTGCGGCATCTTCACCCAGGCGGGCAAAATCAGCCGACAGAAtt                                                                                                         <  2:120740/151‑1 (MQ=255)                                       aCGCAAGGATTTCAGCACCATTGGCCCAATCGTCAGATTGGGAACATAGTGGTTATCCATGACGTCAAAATGCACGACATCAGCGCCAGCTGCCAGGGCTTTTGCGGCATCTTCACCCAGGCGGGCAAAATCAGCCGACAGAATTGAgggg                                                                                                   <  1:870215/151‑1 (MQ=255)                                            aGGATTTCAGCACCATTGGCCCAATCGTCAGATTGGGAACATAGTGGTTATCCATGACGTCAAAATGCACGACATCAGCGCCAGCTGCCAGGGCTTTTGCGGCATCTTCACCCAGGCGGGCAAAATCAGCCGACAGAATTGAGGGGGCAAt                                                                                              >  2:484088/1‑151 (MQ=255)                                            aGGATTTCAGCACCATTGGCCCAATCGTCAGATTGGGAACATAGTGGTTATCCATGACGTCAAAATGCACGACATCAGCGCCAGCTGCCAGGGCTTTTGCGGCATCTTCACCCAGGCGGGCAAAATCAGCCGACAGAATTGAGGGGGCAAt                                                                                              >  2:179704/1‑151 (MQ=255)                                            aGGATTTCAGCACCATTGGCCCAATCGTCAGATTGGGAACATAGTGGTTATCCATGACGTCAAAATGCACGACATCAGCGCCAGCTGCCAGGGCTTTTGCGGCATCTTCACCCAGGCGGGCAAAATCAGCCGACAGAATTGAGGGGGCAAt                                                                                              >  1:673604/1‑151 (MQ=255)                                             ggATTTCAGCACCATTGGCCCAATCGTCAGATTGGGAACATAGTGGTTATCCATGACGTCAAAATGCACGACATCAGCGCCAGCTGCCAGGGCTTTTGCGGCATCTTCACCCAGGCGGGCAAAATCAGCCGACAGAATTGAGGGGGCAATc                                                                                             <  2:545127/151‑1 (MQ=255)                                                  tCAGCACCATTGGCCCAATCGTCAGATTGGGAACATAGTGGTTATCCATGACGTCAAAATGCACGACATCAGCGCCAGCTGCCAGGGCTTTTGCGGCATCTTCACCCAGGCGGGCAAAATCAGCCGACAGAATTGAGGGGGCAATCAAAt                                                                                         <  2:570263/150‑1 (MQ=255)                                                        ccATTGGCCCAATCGTCAGATTGTGAACATAGTGGTTATCCATGACGTCAAAATGCACGACATCAGAGCCAGCTGCCAGGGCTTTTGCGGCATCTTAACCCAGGCGGGCAAAATCAGCCGACAGAATTGAGGGGGCAATCAAATACTGttt                                                                                  <  1:793610/151‑1 (MQ=255)                                                        ccATTGGCCCAATCGTCAGATTGGGAACATAGTGGTTATCCATGACGTCAAAATGCACGACATCAGCGCCAGCTGCCAGGGCTTTTGCGGCATCTTCACCCAGGCGGGCAAAATCAGCCGACAGAATTGAGGGGGCAATCAAATACTGttt                                                                                  >  2:772743/1‑151 (MQ=255)                                                         cATTGGCCCAATCGTCAGATTGGGAACATAGTGGTTATCCATGACGTCAAAATGCACGACATCAGCGCCAGCTGCCAGGGCTTTTGCGGCATCTTCACCCAGGCGGGCAAAATCAGCCGACAGAATTGAGGGGGCAATCAAATACTGTTTc                                                                                 <  2:765849/151‑1 (MQ=255)                                                         cATTGGCCCAATCGTCAGATTGGGAACATAGTGGTTATCCATGACGTCAAAATGCACGACATCAGCGCCAGCTGCCAGGGCTTTTGCGGCATCTTCACCCAGGCGGGCAAAATCAGCCGACAGAATTGAGGGGGCAATCAAATACTGTTTc                                                                                 <  1:164675/151‑1 (MQ=255)                                                         cATTGGCCCAATCGTCAGATTGGGAACATAGTGGTTATCCATGACGTCAAAATGCACGACATCAGCGCCAGCTGCCAGGGCTTTTGCGGCATCTTCACCCAGGCGGGCAAAATCAGCCGACAGAATTGAGGGGGCAATCAAATACTGTTTc                                                                                 >  1:406510/1‑151 (MQ=255)                                                          aTTGGCCCAATCGTCAGATTGGGAACATAGTGGTTATCCATGACGTCAAAATGCACGACATCAGCGCCAGCTGCCAGGGCTTTTGCGGCATCTTCACCCAGGCGGGCAAAATAAGCCGACAGAATTGAGGGGGCAATCAAATACTGTTTc                                                                                 >  2:257104/1‑150 (MQ=255)                                                             ggCCCAATCGTCAGATTGGGAACATAGTGGTTATCCATGACGTCAAAATGCACGACATCAGCGCCAGCTGCCAGGGCTTTTGCGGCATCTTCACCCAGGCGGGCAAAATCAGCCGACAGAATTGAGGGGGCAATCAAATACTGTTTCATcc                                                                             <  1:38230/151‑1 (MQ=255)                                                              gCCCAATCGTCAGATTGGGAACATAGTGGTTATCCATGACGTCAAAATGCACGACATCAGCGCCAGCTGCCAGGGCTTTTGCGGCATCTTCACCCAGGCGGGCAAAATCAGCCGACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCg                                                                            <  1:439660/151‑1 (MQ=255)                                                               cccAATCGTCAGATTGGGAACATAGTGGTTATCCATGACGTCAAAATGCACGACATCAGCGCCAGCTGCCAGGGCTTTTGCGGCATCTTCACCCAGGCGGGCAAAATCAGCCGACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGc                                                                           >  1:547100/1‑151 (MQ=255)                                                                        cAGATTGGGAACATAGTGGTTATCCATGACGTCAAAATGCACGACATCAGCGCCAGCTGCCAGGGCTTTTGCGGCATCTTCACCCAGGCGGGCAAAATCAGCCGACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCtt                                                                   <  1:332454/150‑1 (MQ=255)                                                                        cAGATTGGGAACATAGTGGTTATCCATGACGTCAAAATGCACGACATCAGCGCCAGCTGCCAGGGCTTTTGCGGCATCTTCACCCAGGCGGGCAAAATCAGCCGACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTg                                                                  <  2:22041/151‑1 (MQ=255)                                                                         aGATTGGGAACATAGTGGTTATCCATGACGTCAAAATGCACGACATCAGCGCCAGCTGCCAGGGCTTTTGCGGCATCTTCACCCAGGCGGGCAAAATCAGCCGACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTga                                                                 <  2:420077/151‑1 (MQ=255)                                                                          gATTGGGAACATAGTGGTTATCCATGACGTCAAAATGCACGACATCAGCGCCAGCTGCCAGGGCTTTTGCGGCATCTTCACCCAGGCGGGCAAAATCAGCCGACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTgag                                                                <  2:195618/151‑1 (MQ=255)                                                                           aTTTGGAACATAGTGGTTATCCATGACGTCAAAATGCACGACATCAGCGCCAGCTGCCAGGGCTTTTGCGGCATCTTCACCCAGGCGGGCAAAATCAGCCGACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTcc                                                                     >  2:624814/1‑145 (MQ=255)                                                                             tGGGAACATAGTGGTTATCCATGACGTCAAAATGCACGACATCAGCGCCAGCTGCCAGGGCTTTTGCGGCATCTTCACCCAGGCGGGCAAAATCAGCCGACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGaa                                                              <  1:179728/150‑1 (MQ=255)                                                                                  aCATAGTGGTTATCCATGACGTCAAAATGCACGACATCAGCGCCAGCTGCCAGGGCTTTTGCGGCATCTTCACCCAGGCGGGCAAAATCAGCCGACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTAttt                                                        >  2:820377/1‑151 (MQ=255)                                                                                    aTAGTGGTTATCCATGACGTCAAAATGCACGACATCAGCGCCAGCTGCCAGGGCTTTTGCGGCATCTTCACCCAGGCGGGCAAAATCAGCCGACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTAttttt                                                      <  2:556303/151‑1 (MQ=255)                                                                                    aTAGTGGTTATCCATGACGTCAAAATGCACGACATCAGCGCCAGCTGCCAGGGCTTTTGCGGCATCTTCACCCAGGCGGGCAAAATCAGCCGACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTAttttt                                                      <  2:39679/151‑1 (MQ=255)                                                                                      aGTGGTTATCCATGACGTCAAAATGCACGACATCAGCGCCAGCTGCCAGGGCTTTTGCGGCATCTTCACCCAGGCGGGCAAAATCAGCCGACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTc                                                    <  1:391326/151‑1 (MQ=255)                                                                                       gTGGTTATCCATGACGTCAAAATGCACGACATCAGCGCCAGCTGCCAGGGCTTTTGCGGCATCTTCACCCAGGCGGGCAAAATCAGCCGACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGATtttttttt                                                      >  1:530017/1‑148 (MQ=255)                                                                                       gTGGTTATCCATGACGTCAAAATGCACGACATCAGCGCCAGCTGCCAGGGCTTTTGCGGCATCTTCACCCAGGCGGGCAAAATCAGCCGACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTcg                                                   >  1:537396/1‑151 (MQ=255)                                                                                           ttATCCATGACGTCAAAATGCACGACATCAGCGCCAGCTGCCAGGGCTTTTGCGGCATCTTCACCCAGGCGGGCAAAATCAGCCGACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCggg                                               >  2:349373/1‑151 (MQ=255)                                                                                                   gACGTCAAAATGCACGACATCAGCGCCAGCTGCCAGGGCTTTTGCGGCATCTTCACCCAGGCGGGCAAAATCAGCCGACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACg                                        >  1:536333/1‑150 (MQ=255)                                                                                                     cGTCAAAATGCACGACATCAGCGCCAGCTGCCAGGGCTTTTGCGGCATCTTCACCCAGGCGGGCAAAATCAGCCGACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACt                                     <  1:321528/151‑1 (MQ=255)                                                                                                        cAAAATGCACGACATCAGCGCCAGCTGCCAGGGCTTTTGCGGCATCTTCACCCAGGCGGGCAAAATCAGCCGACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTcc                                   >  2:873726/1‑150 (MQ=255)                                                                                                          aaaTGCACGACATCAGCGCCAGCTGCCAGGGCTTTTGCGGCATCTTCACCCAGGCGGGCAAAATCAGCCGACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTgg                                <  1:31213/151‑1 (MQ=255)                                                                                                                    cATCAGCGCCAGCTGCCAGGGCTTTTGCGGCATCTTCACCCAGGCGGGCAAAATCAGCCGACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACaa                       >  2:210901/1‑150 (MQ=255)                                                                                                                      tCAGCGCCAGCTGCCAGGGCTTTTGCGGCATCTTCACCCAGGCGGGCAAAATCAGCCGACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACAAAGc                    >  1:845832/1‑151 (MQ=255)                                                                                                                      tCAGCGCCAGCTGCCAGGGCTTTTGCGGCATCTTCACCCAGGCGGGCAAAATCAGCCGACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACAAAGc                    >  1:785368/1‑151 (MQ=255)                                                                                                                        aGCGCCAGCTGCCAGGGCTTTTGCGGCATCTTCACCCAGGCGGGCAAAATCAGCCGACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACAAAGcc                   >  1:580444/1‑150 (MQ=255)                                                                                                                        aGCGCCAGCTGCCAGGGCTTTTGCGGCATCTTCACCCAGGCGGGCAAAATCAGCCGACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACAAAGCca                  <  2:432367/151‑1 (MQ=255)                                                                                                                         gcgcCAGCTGCCAGGGCTTTTGCGGCATCTTCACCCAGGCGGGCAAAATCAGCCGACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACAAAGCcag                 <  1:281549/151‑1 (MQ=255)                                                                                                                           gcCAGCTGCCAGGGCTTTTGCGGCATCTTCACCCAGGCGGGCAAAATCAGCCGACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACAAAGCcagca               <  2:435141/151‑1 (MQ=255)                                                                                                                               gCTGCCAGGGCTTTTGCGGCATCTTCACCCAGGCGGGCAAAATCAGCCGACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACAAAGCCAGCAGTTc           <  2:449675/151‑1 (MQ=255)                                                                                                                                   ccAGGGCTTTTGCGGCATCTTCACCCAGGCGGGCAAAATCAGCCGACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACAAAGCCAGCAGTTCGTcc       <  2:792129/151‑1 (MQ=255)                                                                                                                                        gCTTTTGCGGCATCTTCACCCAGGCGGGCAAAATCAGCCGACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACAAAGCCAGCAGTTCGTCCACCtt  <  2:631443/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                             ATCAGGTGTACGTCGATAGGGGCGGTAATGCCATAGTTACGCAAGGATTTCAGCACCATTGGCCCAATCGTCAGATTGGGAACATAGTGGTTATCCATGACGTCAAAATGCACGACATCAGCGCCAGCTGCCAGGGCTTTTGCGGTATCTTCACCCAGGCGGGCAAAATCAGCCGACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACAAAGCCAGCAGTTCGTCCACCTT  >  NC_000913/3514851‑3515136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑