Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,538,396	A→G	T338A (ACT→GCT)	yhgF →	putative transcriptional accessory protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,538,396	0	A	G	100.0%	185.0 / NA	52	T338A (ACT→GCT)	yhgF	putative transcriptional accessory protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/26);  total (26/26)

GCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACTGGCAAACTGGTGGCGACCGAC‑ACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGAACTGGT  >  NC_000913/3538251‑3538532                                                                                                                                                  |                                                                                                                                           gCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTggg                                                                                                                                       <  1:287800/151‑1 (MQ=255) gCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTgg                                                                                                                                        >  2:439788/1‑150 (MQ=255)    tCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGt                                                                                                                                     >  1:457494/1‑150 (MQ=255)      tGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTaa                                                                                                                                   >  2:110690/1‑150 (MQ=255)        gAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTaaa                                                                                                                                  >  2:12468/1‑149 (MQ=255)                  tGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCggtg                                                                                                                        >  1:219942/1‑149 (MQ=255)                   gATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCggtggt                                                                                                                      <  1:69631/150‑1 (MQ=255)                       ggCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATg                                                                                                                 <  1:887565/151‑1 (MQ=255)                            cGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCggtggggggaa                                                                                                                 >  2:458758/1‑140 (MQ=255)                             gTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACTg                                                                                                          <  1:265076/151‑1 (MQ=255)                                      cGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACTGGCAAACtgg                                                                                                 <  2:219085/151‑1 (MQ=255)                                       gTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGGCGATGCC‑CCCGGGaaa                                                                                                     >  2:128480/1‑146 (MQ=255)                                           ggAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGGCGATGCC‑CCTGGCAAACtggggggg                                                                                            >  1:796942/1‑149 (MQ=255)                                                aTGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGAGGCC‑ACTCGCAAACtggtg                                                                                               >  1:831084/1‑143 (MQ=255)                                                 tGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGGTGCC‑ACTTGCAAACTGGTGGcg                                                                                            >  1:145274/1‑145 (MQ=255)                                                  gAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACTGGCAAACTGGTGGCGACCGac‑a                                                                                    <  1:663440/151‑1 (MQ=255)                                                    aGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACCGGCCAACAGGGGGGGACCGAC‑Acc                                                                                  >  1:886634/1‑151 (MQ=255)                                                     gCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACTGGCAAACTGGTGGCGACCGAC‑Acc                                                                                  <  2:773714/150‑1 (MQ=255)                                                     gCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGGTGGTGCC‑CCTGGCCAACCTGTGGggaccggc                                                                                      >  2:369240/1‑145 (MQ=255)                                                      cAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGGTGCC‑ACTGGCAAACTGGTGGGGCCCGCC‑Acc                                                                                  >  1:555646/1‑149 (MQ=255)                                                       aaTCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGGGGGCGGGGGC‑CCTGGGAAACTGGGGGGGGCCGCC‑Acc                                                                                  >  2:86691/1‑148 (MQ=255)                                                           aaCGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGGGGGCGGTGCC‑CCTGGGAAACCGGGGGCGGCCCCC‑Acccatt                                                                              >  2:714433/1‑144 (MQ=255)                                                                  ttGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACTGGCAAACTGGTGGCGACCGAC‑ACCATTTACCCGcac                                                                      <  1:41751/149‑1 (MQ=255)                                                                    gCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGCTGACAGTGCC‑CCTGGGAAACTGGGGGCGCGCGACAACCATTAAccacacccc                                                                    >  1:703386/1‑143 (MQ=255)                                                                       cGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGGCGGGGCCTCCCGGCAAACGGGGGGGGACCG‑C‑ACCAtttttccccccccc                                                                   >  2:219043/1‑136 (MQ=255)                                                                        gTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGGCGATGCC‑ACCTGCAAACTGGTGGGGGCCGGC‑ACCCTTTTCCCGCACAcc                                                                   >  1:145642/1‑146 (MQ=255)                                                                        gTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGCGTAAAAGTGGCGGTGGGCGGTGGC‑CCACGCAAAACGGTTGCGACCGAC‑ACCACTTATCCGcccgcccgcc                                                               >  2:440887/1‑141 (MQ=255)                                                                          aaCCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACTGGCAAACTGGTGGCGACCGAC‑ACCATTTACCCGCACACCGGACAgg                                                            <  2:664538/151‑1 (MQ=255)                                                                               gCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACTGGCAAACTGGTGGCGACCGAC‑ACCATTTACCCGCACACCGGACAGGCCGCa                                                       <  2:457447/151‑1 (MQ=255)                                                                                        gctgATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACTGGCAAACTGGTGGCGACCGAC‑ACCATTTACCCGCACACCGGACAGGCCGCaaaa                                                    <  2:839967/145‑1 (MQ=255)                                                                                        gctgATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACTGGCAAACTGGTGGCGACCGAC‑ACCATTTACCCGCACACCGGACAGGCCGCAAAagcagc                                               <  2:395999/150‑1 (MQ=255)                                                                                          tgATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGGCGGTGCC‑CCCGGCCAACAGGGGGGGGCCGAC‑ACCATTTTCCCGCCCCCCggggcgggcgccaaaa                                                   >  2:826000/1‑130 (MQ=255)                                                                                           gATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACTGGCAAACTGGTGGCGACCGAC‑ACCATTTACCCGCACCCCGGACAGGCCGCAAAAGCAGCGATg                                           <  2:389752/151‑1 (MQ=255)                                                                                                     ccTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGACGATGCC‑ACTTGCAAAATGGTGGCGACCGAC‑AACCTTTACCCGCCCACCCGGCAGGCCCCAAAAACAGCGATGaac                                        >  1:883498/1‑142 (MQ=255)                                                                                                        gCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACTGGCAAACTGGTGGCGACCGAC‑ACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTtgctgc                                <  2:184700/149‑1 (MQ=255)                                                                                                        gCCGGACGGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACTGGCAAACGGGTGGCGACCGAC‑ACCATTTCCCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTtgctgc                                <  2:880360/149‑1 (MQ=255)                                                                                                          cGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑CCTGGCAAACTGGTGGCGACCGAC‑ACCATTTACCCGCACACCGGACAGGCCGCAAAAACAGCGATGACCggt                                     >  1:544675/1‑140 (MQ=255)                                                                                                          cGGACTGCGTGCAACGATGGGCCGCGATCCGGGTCGGCGTGCTGGGGTAAAAGTGGCGGGGGTCGATGCC‑ACTGGCAAACTGGTGGCGACCGAC‑ACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCt                             <  2:410057/150‑1 (MQ=255)                                                                                                           ggACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACTGGCAAACTGGTGGCGACCGAC‑ACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCtgt                           <  1:447053/151‑1 (MQ=255)                                                                                                             aCTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACTGGCAAACTGGTGGCGACCGAC‑ACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCtgtgt                         <  2:260028/151‑1 (MQ=255)                                                                                                             aCTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGGCGGTGCC‑ACTGGCAAACTGGTGGGGCCCGGC‑ACCACTTAACCGCACACCCGACAGGCCGCCAAAACAGCGCGGACCCTTGGTGCGCtgtg                          >  2:150597/1‑150 (MQ=255)                                                                                                                 cGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACTGGCAAACTGGTGGCGACCGAC‑ACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCtgtgtg                        <  2:428549/148‑1 (MQ=255)                                                                                                                   tGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑CCTGGCAAACTGGTGGCGGCCGAC‑ACCCATTAACCGCCCACCGGGCAGGCCGCAAAAACAGCGGTGACCGTTGCTGCGCtgtg                          >  2:176228/1‑144 (MQ=255)                                                                                                                   tGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACTGGCAAACTGGTGGCGACCGAC‑ACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCtgtg                          <  1:176252/144‑1 (MQ=255)                                                                                                                      aaCGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑CCTGGCAAAATGGGGGCGGCCGAC‑ACCAATTAACCGCACACCCGACAGGGCGCAAAAAAAGCGGTGACCGTTGCTGCGCTGTGTGgaaaa                   >  2:508207/1‑148 (MQ=255)                                                                                                                       aCGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACTGGCAAACTGGTGGCGACCGAC‑ACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATa               <  1:656653/151‑1 (MQ=255)                                                                                                                         gATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACTGGCAAACTGGTGGCGACCGAC‑ACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAAc             <  1:785353/151‑1 (MQ=255)                                                                                                                          aTGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACTGGCAAACTGGTGGCGACCGAC‑ACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACg            <  1:599051/151‑1 (MQ=255)                                                                                                                            gggCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACTGGCAAACTGGTGGCGACCGAC‑ACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGtt          <  2:608084/151‑1 (MQ=255)                                                                                                                            gggCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACTGGCAAACTGGTGGCGACCGAC‑ACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGt           <  2:160992/150‑1 (MQ=255)                                                                                                                                   gATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACCGGCAAACTGGTGGCGACCGAC‑ACCATTTACCCGCACACCCGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAAAATAACGTTGAAATgg   >  1:125667/1‑151 (MQ=255)                                                                                                                                    aTCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACTGGCAAACTGGTGGCGACCGAC‑ACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGAACTGGt  <  1:175026/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                           GCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACTGGCAAACTGGTGGCGACCGAC‑ACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGAACTGGT  >  NC_000913/3538251‑3538532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑