Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,547,938	T→C	intergenic (+63/+48)	gntT → / ← malQ	gluconate transporter, high‑affinity GNT I system/4‑alpha‑glucanotransferase (amylomaltase)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,547,938	0	T	C	100.0%	151.0 / NA	42	intergenic (+63/+48)	gntT/malQ	gluconate transporter, high‑affinity GNT I system/4‑alpha‑glucanotransferase (amylomaltase)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/22);  total (20/22)

ATCAAATCCTGGTCGATGCTGGAAACCATTATCTCGGTGTGCGGTCTGGTAGGCTGTCTGCTGCTGAATATGGTGATTTGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTCTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGA  >  NC_000913/3547795‑3548081                                                                                                                                                |                                                                                                                                                aTCAAATCCTGGTCGATGCTGGAAACCATTATCTCGGTGTGCGGTCTGGTAGGCTGTCTGCTGCTGAATATGGTGATTTGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGCAGGCCgg                                                                                                                                          >  1:353658/1‑151 (MQ=255)    aaaTCCTGGTCGATGCTGGAAACCATTATCTCGGTGTGCGGTCTGGTAGGCTGTCTGCTGCTGAATATGGTGATTTGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGCAGGCCGGAt                                                                                                                                        >  1:830552/1‑150 (MQ=255)     aaTCCTGGTCGATGCTGGAAACCATTATCTCGGTGTGCGGTCTGGTAGGCTGTCTGCTGCTGAATATGGTGATTTGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGCAGGCCGGATaa                                                                                                                                      <  2:411642/151‑1 (MQ=255)     aaTCCTGGTCGATGCTGGAAACCATTATCTCGGTGTGCGGTCTGGTAGGCTGTCTGCTGCTGAATATGGTGATTTGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGCAGGCCGGATaa                                                                                                                                      <  1:441113/151‑1 (MQ=255)                aTGCTGGAAACCATTATCTCGGTGTGCGGTCTGGTAGGCTGTCTGCTGCTGAATATGGTGATTTGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGCAGGCCGGATAAGACGCGTCa                                                                                                                             >  2:609998/1‑149 (MQ=255)                aTGCTGGAAACCATTATCTCGGTGTGCGGTCTGGTAGGCTGTCTGCTGCTGAATATGGTGATTTGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGCCCTACGATTCGGGATGAATTAGCACGCCGGATATGACGCGTCa                                                                                                                             >  1:704707/1‑149 (MQ=255)                  gCTGGAAACCATTATCTCGGTGTGCGGTCTGGTAGGCTGTCTGCTGCTGAATATGGTGATTTGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGCAGGCCGGATAAGACGCGTCAAgca                                                                                                                         <  2:541962/151‑1 (MQ=255)                  gCTGGAAACCATTATCTCGGTGTGCGGTCTGGTAGGCTGTCTGCTGCTGAATATGGTGATTTGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGCAGGCCGGATAAGACGCGTCAAgc                                                                                                                          <  2:641683/150‑1 (MQ=255)                  gCTGGAAACCATTATCTCGGTGTGCGGTCTGGTAGGCTGTCTGCTGCTGAATATGGTGATTTGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGCAGGCCGGATAAGACGCGTCAAgc                                                                                                                          >  1:854837/1‑150 (MQ=255)                     ggAAACCATTATCTCGGTGTGCGGTCTGGTAGGCTGTCTGCTGCTGAATATGGTGATTTGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGCAGGCCGGATAAGACGCGTCAAgcatcg                                                                                                                      >  1:861837/1‑151 (MQ=255)                                   cGGTGTGCGGTCTGGTAGGCTGTCTGCTGCTGAATATGGTGATTTGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGCAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAAc                                                                                                        >  1:836645/1‑151 (MQ=255)                                     gtgtGCGGTCTGGTAGGCTGTCTGCTGCTGAATATGGTGATTTGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGCAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCg                                                                                                      <  1:328721/151‑1 (MQ=255)                                       gtgCGGTCTGGTAGGCTGTCTGCTGCTGAATATGGTGATTTGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGCAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCa                                                                                                    <  1:836314/151‑1 (MQ=255)                                         gCGGTCTGGTAGGCTGTCTGCTGCTGAATATGGTGATTTGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGCAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCAc                                                                                                   <  1:163481/150‑1 (MQ=255)                                         gCGGTCTGGTAGGCTGTCTGCTGCTGAATATGGTGATTTGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGCAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCAc                                                                                                   <  1:351276/150‑1 (MQ=255)                                            gTCTGGTAGGCTGTCTGCTGCTGAATATGGTGATTTGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGCAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCActct                                                                                                >  2:688204/1‑150 (MQ=255)                                               tGGTAGGCTGTCTGCTGCTGAATATGGTGATTTGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGCAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTCTActt                                                                                            >  2:808411/1‑151 (MQ=255)                                                            gctgctgAATATGGTGATTTGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGCAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTCTACTTCTTCTTCGCt                                                                                  <  1:213431/148‑1 (MQ=255)                                                              tgctgAATATGGTGATTTGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGCAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTCTACTTCTTCTTCGCTGCAGc                                                                             >  2:892759/1‑151 (MQ=255)                                                                            aTTTGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGCAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTCTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGt                                                               >  1:554401/1‑151 (MQ=255)                                                                               tGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGCAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTCTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTcc                                                             <  2:665741/150‑1 (MQ=255)                                                                               tGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGCAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTCTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTcc                                                             >  2:277061/1‑150 (MQ=255)                                                                                         gactgCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGCAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTCTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTc                                                    >  1:247602/1‑149 (MQ=255)                                                                                         gactgCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGCAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTCTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTc                                                    <  2:392664/149‑1 (MQ=255)                                                                                         gactgCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGCAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTCTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAg                                                  <  1:264987/151‑1 (MQ=255)                                                                                                     cggcgTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGCAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTCTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCAc                                      >  2:89555/1‑151 (MQ=255)                                                                                                      ggcgTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGCAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTCTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACg                                     >  1:901371/1‑151 (MQ=255)                                                                                                      ggcgTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGCAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTCTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACg                                     >  1:318740/1‑151 (MQ=255)                                                                                                        cgTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGCAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTCTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGcc                                   <  1:89577/151‑1 (MQ=255)                                                                                                         gTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGCAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTCTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCca                                  <  1:25878/151‑1 (MQ=255)                                                                                                          tGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGCAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTCTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCcat                                 >  1:416729/1‑151 (MQ=255)                                                                                                                      ccGGCCTACGATTCGGGATGAATTAGCAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTCTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACa                     <  1:683002/151‑1 (MQ=255)                                                                                                                       cGGCCTACGATTCGGGATGAATTAGCAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTCTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACAt                    <  2:705669/151‑1 (MQ=255)                                                                                                                          ccTACGATTCGGGATGAATTAGCAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTCTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGa                 <  1:123349/151‑1 (MQ=255)                                                                                                                             aCGATTCGGGATGAATTAGCAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTCTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTc              >  1:811974/1‑151 (MQ=255)                                                                                                                              cGATTCGGGATGAATTAGCAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTCTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCa             <  1:636408/151‑1 (MQ=255)                                                                                                                              cGATTCGGGATGAATTAGCAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTCTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCa             <  1:602783/151‑1 (MQ=255)                                                                                                                                   cGGGATGAATTAGCAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTCTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGt        >  2:183440/1‑151 (MQ=255)                                                                                                                                    gggATGAATTAGCAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTCTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGtt       <  2:302172/151‑1 (MQ=255)                                                                                                                                    gggATGAATTAGCAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTCTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGt        >  2:478507/1‑150 (MQ=255)                                                                                                                                         gAATTAGCAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTCTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGa  <  1:258037/151‑1 (MQ=255)                                                                                                                                          aaTTAGCAGGCCGTATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTCTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCgg   <  1:375972/149‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                ATCAAATCCTGGTCGATGCTGGAAACCATTATCTCGGTGTGCGGTCTGGTAGGCTGTCTGCTGCTGAATATGGTGATTTGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTCTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGA  >  NC_000913/3547795‑3548081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑