| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,555,945 | A→G | V302A (GTC→GCC) | rtcA ← | RNA 3'‑terminal phosphate cyclase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,555,945 | 0 | A | G | 97.7% | 133.0 / NA | 44 | V302A (GTC→GCC) | rtcA | RNA 3'‑terminal phosphate cyclase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (26/17); minor base C (0/1); total (26/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.09e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCG > NC_000913/3555798‑3556084 | cGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGGCCg > 1:740451/1‑151 (MQ=255)cGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGGCCg > 1:525648/1‑151 (MQ=255) tAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGGCCGTa > 1:180415/1‑149 (MQ=255) cgcgCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGGCCGTAAATTc > 2:117357/1‑151 (MQ=255) aGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGGCCGTAAATTCCCCcgc < 2:426346/151‑1 (MQ=255) gCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGGCCGTAAATTCCCCCGcgcccgcc > 1:183912/1‑151 (MQ=255) tACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGGCCGTAAATTCCCCCGCGCCCGCCAgc > 1:705429/1‑151 (MQ=255) catTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGGCCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGc > 1:748552/1‑150 (MQ=255) cAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGGCCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCa < 1:686261/150‑1 (MQ=255) cACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGGCCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGt < 1:403230/149‑1 (MQ=255) cccGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGGCCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGc < 2:5770/151‑1 (MQ=255) aCGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGGCCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATAtt > 2:693644/1‑151 (MQ=255) ccATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGGCCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTccc < 1:466475/151‑1 (MQ=255) tGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATGTTGGTCAGCAGATGGCATGAGGGATGGGCGGCCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCAcc > 2:62242/1‑151 (MQ=255) gTTTCTATCAAACTAAACCGCCCCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGGCCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCAc < 2:772615/149‑1 (MQ=255) gTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGGCCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCAccg > 1:632571/1‑151 (MQ=255) cTATCAAACTAAACCGCCCCGGCAAGAAACGCTCCACCCCCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGCCCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCAccgcc < 1:537283/149‑1 (MQ=255) cTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGGCCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCAccgccgc > 1:365170/1‑151 (MQ=255) aTCAAACTAACCCGCCCCGGCAAGAAACGCTCCCCCCCCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGGGGCCGTAAATTCCCCCGCCCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCAccgccg < 2:740401/148‑1 (MQ=255) aTCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGGCCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCAccgccgccg > 2:490323/1‑151 (MQ=255) tCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGGCCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCAccgccgccg > 2:569782/1‑150 (MQ=255) cAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGGCCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTg > 1:453301/1‑151 (MQ=255) aaaCCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGGCCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGcc > 1:831731/1‑151 (MQ=255) cGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGGCCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGt > 2:813787/1‑151 (MQ=255) gCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGGCCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGt > 2:652166/1‑150 (MQ=255) cACCGGCAAGAAACGCTCCCCCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGGCCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGt < 2:26317/149‑1 (MQ=255) cAAGAAACGCTCCCCCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGGCCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGttt < 2:106202/150‑1 (MQ=255) aaGAAACGCTCCACCCCCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGGCCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCa < 2:576170/151‑1 (MQ=255) aaGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGGCCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTc > 1:800466/1‑150 (MQ=255) cTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGGCCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCttt < 2:793886/150‑1 (MQ=255) cTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGGCCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTc > 2:745607/1‑151 (MQ=255) tCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGGCCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTc > 2:884900/1‑150 (MQ=255) ccaccaCCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGGCCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACctct < 1:756523/146‑1 (MQ=255) caccacCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGGCCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCAcc < 2:872628/151‑1 (MQ=255) accaccGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGGCCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCAcc > 1:183760/1‑150 (MQ=255) caccGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGGCCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAAc > 1:450540/1‑151 (MQ=255) accGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGGCCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACt > 1:808952/1‑151 (MQ=255) gcgATATTGGTCAGCAGATGGCATGAGGGATGGGCGGCCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACtgtg < 2:417877/151‑1 (MQ=255) ggTCAGCAGATGGCATGAGGGATGGGCGGCCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAacc > 1:448933/1‑151 (MQ=255) agATGGCATGAGGGATGGGCGGCCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCg < 1:66571/150‑1 (MQ=255) tGGCATGAGGGATGGGCGGCCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCAc > 2:651467/1‑151 (MQ=255) aTGAGGGATGGGCGGCCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGAc > 1:546849/1‑151 (MQ=255) gAGGGCTTGGCGGCCGAAAACTCCCCGGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCCAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTCTCACCAACTGTGCCGCAACCACCTCGGCTCTGAcgc < 2:709313/151‑1 (MQ=255) gggATGGGCGGCCGTAAATCCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGAcgcg < 2:237062/150‑1 (MQ=255) | CGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCG > NC_000913/3555798‑3556084 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |