Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	443,420	A→G	G74G (GGT→GGC)	yajL ←	oxidative‑stress‑resistance chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	443,420	0	A	G	100.0%	185.4 / NA	53	G74G (GGT→GGC)	yajL	oxidative‑stress‑resistance chaperone
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/25);  total (28/25)

ATTACCAATCGGGAAGATATCGTGCGGCACCAGCACGGTGGCTGGCGCGGCGCAAATAGCCGCGACGATACGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCACCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCG  >  NC_000913/443273‑443563                                                                                                                                                    |                                                                                                                                                atTACCAATCGGGAAGATATCGTGCGGCACCAGCACGGTGGCTGGCGCGGCGCAAATAGCCGCGACGTTACGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGccgcca                                                                                                                                              <  1:187558/151‑1 (MQ=255) atTACCAATCGGGAAGATATCGTGCGGCACCAGCACGGTGGCTGGCGCGGCGCAAATAGCCGCGACGATACGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGccgcca                                                                                                                                              <  2:680735/151‑1 (MQ=255) atTACCAATCGGGAAGATATCGTGCGGCACCAGCACGGTGGCTGGCGCGGCGCAAATAGCCGCGACGATACGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGccgcca                                                                                                                                              <  1:499644/151‑1 (MQ=255)  ttACCAAGCGGGAAGATATCGTGCGTCACCAGCACGGTGGCTGGCGCGGCGCAAATAGCCGCGACGATACGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGccgcca                                                                                                                                              <  1:712769/150‑1 (MQ=255)       aaTCGGGAAGATATCGTGCGGCACCAGCACGGTGGCTGGCGCGGCGCAAATAGCCGCGACGATACGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGgcagc                                                                                                                                        <  2:148129/151‑1 (MQ=255)        aTCGGGAAGATATCGTGCGGCACCAGCACGGTGGCTGGCGCGGCGCAAATAGCCGCGACGATACGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGgcagca                                                                                                                                       <  2:486886/151‑1 (MQ=255)          cGGGAAGATATCGTGCGGCACCAGCACGGTGGCTGGCGCGGCGCAAATAGCCGCGACGATACGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCAc                                                                                                                                      <  1:440444/150‑1 (MQ=255)          cGGGAAGATATCGTGCGGCACCAGCACGGTGGCTGGCGCGGCGCAAATAGCCGCGACGATACGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACg                                                                                                                                     >  2:303921/1‑151 (MQ=255)           gggAAGATATCGTGCGGCACCAGCACGGTGGCTGGCGCGGCGCAAATAGCCGCGACGATACGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACg                                                                                                                                     >  2:657511/1‑150 (MQ=255)             gAAGATATCGTGCGGCACCAGCACGGTGGCTGGCGCGGCGCAAATAGCCGCGACGATACGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATc                                                                                                                                  <  1:222249/151‑1 (MQ=255)             gAAGATATCGTGCGGCACCAGCACGGTGGCTGGCGCGGCGCAAATAGCCGCGACGATACGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATc                                                                                                                                  <  1:423900/151‑1 (MQ=255)                      gTGCGGCACCAGCACGGTGGCTGGCGCGGCGCAAATAGCCGCGACGATACGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCatat                                                                                                                         >  1:897297/1‑151 (MQ=255)                        gCGGCACCAGCACGGTGGCTGGCGCGGCGCAAATAGCCGCGACGATACGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATAtt                                                                                                                        <  2:619169/150‑1 (MQ=255)                        gCGGCACCAGCACGGTGGCTGGCGCGGCGCAAATAGCCGCGACGATACGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATAtt                                                                                                                        <  2:735054/150‑1 (MQ=255)                             aCCAGCACGGTGGCTGGCGCGGCGCAAATAGCCGCGACGATACGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCAt                                                                                                                  >  1:518039/1‑151 (MQ=255)                              ccAGCACGGTGGCTGGCGCGGCGCAAATAGCCGCGACGATACGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCAt                                                                                                                  <  2:390973/150‑1 (MQ=255)                                     ggTGGCTGGCGCGGCGCAAATAGCCGCGACGATACGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACt                                                                                                          >  2:137217/1‑151 (MQ=255)                                      gTGGCTGGCGCGGCGCAAATAGCCGCGACGATACGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACtt                                                                                                         >  1:750312/1‑151 (MQ=255)                                         gctggcGCGGCGCAAATAGCCGCGACGATACGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCg                                                                                                                      <  1:448656/135‑1 (MQ=255)                                         gctggcGCGGCGCAAATAGCCGCGACGATACGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCg                                                                                                                      >  2:448609/1‑135 (MQ=255)                                         gctggcGCGGCGCAAATAGCCGCGACGATACGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGa                                                                                                      <  1:250096/151‑1 (MQ=255)                                          ctggcGCGGCGCAAATAGCCGCGACGATACGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGAc                                                                                                     >  2:655164/1‑151 (MQ=255)                                           tggcGCGGCGCAAATAGCCGCGACGATACGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGAcc                                                                                                    >  1:449590/1‑151 (MQ=255)                                            ggcgcggcgcAAATAGCCGCGACGATACGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGAc                                                                                                     <  2:393150/149‑1 (MQ=255)                                                   cgcAAATAGCCGCGACGATACGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGgcgc                                                                                            >  1:904808/1‑151 (MQ=255)                                                          aGCCGCGACGATACGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGcc                                                                                     >  2:885040/1‑151 (MQ=255)                                                          aGCCGCGACGATACGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGcc                                                                                     >  1:268107/1‑151 (MQ=255)                                                           gCCGCGACGATACGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGcc                                                                                     >  2:745805/1‑150 (MQ=255)                                                                  cgaTACGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCtt                                                                             >  2:338932/1‑151 (MQ=255)                                                                      aCGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCAcg                                                                         <  2:35913/151‑1 (MQ=255)                                                                                cGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGc                                                               >  2:19910/1‑151 (MQ=255)                                                                                       aCTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAAt                                                        >  2:357708/1‑151 (MQ=255)                                                                                             tAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGcc                                                    >  2:711877/1‑149 (MQ=255)                                                                                              aaCGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAg                                                  <  1:639482/150‑1 (MQ=255)                                                                                                cGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGtt                                               >  1:904/1‑151 (MQ=255)                                                                                                 ggTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAAt                                                        >  1:727007/1‑141 (MQ=255)                                                                                                 ggTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAAt                                                        <  2:726958/141‑1 (MQ=255)                                                                                                 ggTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTa                                              <  1:472763/151‑1 (MQ=255)                                                                                                     tCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCa                                           <  1:298813/150‑1 (MQ=255)                                                                                                     tCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCAt                                          >  2:724306/1‑151 (MQ=255)                                                                                                       aaCCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCg                                        >  2:750336/1‑151 (MQ=255)                                                                                                         ccAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCt                                      >  1:82692/1‑151 (MQ=255)                                                                                                           agcagAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTgg                                    >  1:705079/1‑151 (MQ=255)                                                                                                                   gCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTg                            <  1:573159/151‑1 (MQ=255)                                                                                                                      aTCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCa                         <  2:808876/151‑1 (MQ=255)                                                                                                                       tCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCa                         <  2:649657/150‑1 (MQ=255)                                                                                                                        cgcgAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAgt                       <  2:556369/151‑1 (MQ=255)                                                                                                                           gAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAgtggt                    >  1:595224/1‑151 (MQ=255)                                                                                                                             aaaCACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAgtggtg                   >  2:87726/1‑150 (MQ=255)                                                                                                                             aaaCACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGa                  <  2:475180/151‑1 (MQ=255)                                                                                                                                 acTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACttt              <  1:851297/151‑1 (MQ=255)                                                                                                                                  cTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACttt              <  1:372717/150‑1 (MQ=255)                                                                                                                                    ccGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTg             <  2:530509/149‑1 (MQ=255)                                                                                                                                    ccGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGAt           <  1:227610/151‑1 (MQ=255)                                                                                                                                        gcCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATAccg       >  2:100625/1‑151 (MQ=255)                                                                                                                                         ccTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATAccgc      >  1:209618/1‑151 (MQ=255)                                                                                                                                             tAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCTCCAGGTTACCATGGCTGGCGAAGCTGGCAGTGGTGACTTTGATACCGCcgcg  >  1:674374/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                ATTACCAATCGGGAAGATATCGTGCGGCACCAGCACGGTGGCTGGCGCGGCGCAAATAGCCGCGACGATACGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCACCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCG  >  NC_000913/443273‑443563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑