Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,733,738	T→C	V513A (GTC→GCC)	xylG →	D‑xylose ABC transporter dual domain ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,733,738	0	T	C	100.0%	189.6 / NA	52	V513A (GTC→GCC)	xylG	D‑xylose ABC transporter dual domain ATPase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/25);  total (27/25)

CCTGAAGTGCTCGGCCTTAGCGATCGTGTACTGGTGATGCATGAAGGGAAACTAAAAGCCAACCTGATAAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTACCTGGACCACCGATGGTGCCTACTTAAGCGCCCGT  >  NC_000913/3733596‑3733884                                                                                                                                               |                                                                                                                                                   ccTGAAGTGCTCGGCCTTAGCGATCGTGTACTGGTGATGCATGAAGGGAAACTAAAAGCCAACCTGATAAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTg                                                                                                                                            >  2:387908/1‑151 (MQ=255)   tGAAGTGCTCGGCCTTAGCGATCGTGTACTGGTGATGCATGAAGGGAAACTAAAAGCCAACCTGATAAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGa                                                                                                                                           >  1:733605/1‑150 (MQ=255)    gAAGTGCTCGGCCTTAGCGATCGTGTACTGGTGATGCATGAAGGGAAACTAAAAGCCAACCTGATAAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGaa                                                                                                                                          >  2:879319/1‑150 (MQ=255)     aaGTGCTCGGCCTTAGCGATCGTGTACTGGTGATGCATGAAGGGAAACTAAAAGCCAACCTGATAAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAAt                                                                                                                                        >  1:880212/1‑151 (MQ=255)         gCTCGGCCTTAGCGATCGTGTACTGGTGATGCATGAAGGGAAACTAAAAGCCAACCTGATAAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTgg                                                                                                                                     <  2:226025/150‑1 (MQ=255)            cGGCCTTAGCGATCGTGTACTGGTGATGCATGAAGGGAAACTAAAAGCCAACCTGATAAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCg                                                                                                                                  <  1:542203/150‑1 (MQ=255)             ggCCTTAGCGATCGTGTACTGGTGATGCATGAAGGGAAACTAAAAGCCAACCTGATAAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTa                                                                                                                                <  1:325851/151‑1 (MQ=255)                 ttAGCGATCGTGTACTGGTGATGCATGAAGGGAAACTAAAAGCCAACCTGATAAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCg                                                                                                                             >  2:613402/1‑150 (MQ=255)                  tAGCGATCGTGTACTGGTGATGCATGAAGGGAAACTAAAAGCCAACCTGATAAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCg                                                                                                                             <  1:685467/149‑1 (MQ=255)                   aGCGATCGTGTACTGGTGATGCATGAAGGGAAACTAAAAGCCAACCTGATAAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGAc                                                                                                                           >  1:88675/1‑150 (MQ=255)                     cGATCGTGTACTGGTGATGCATGAAGGGAAACTAAAAGCCAACCTGATAAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATc                                                                                                                        <  2:750364/151‑1 (MQ=255)                     cGATCGTGTACTGGTGATGCATGAAGGGAAACTAAAAGCCAACCTGATAAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATc                                                                                                                        <  1:349287/151‑1 (MQ=255)                         cGTGTACTGGTGATGCATGAAGGGAAACTAAAAGCCAACCTGATAAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGt                                                                                                                    >  2:760366/1‑151 (MQ=255)                                          tGAAGGGAAACTAAAAGCCAACCTGATAAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGa                                                                                                    >  1:361682/1‑150 (MQ=255)                                           gAAGGGAAACTAAAAGCCAACCTGATAAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGaaa                                                                                                  <  2:471027/151‑1 (MQ=255)                                            aaGGGAAACTAAAAGCCAACCTGATAAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAAt                                                                                                 >  1:397377/1‑151 (MQ=255)                                             aGGGAAACTAAAAGCCAACCTGATAAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATc                                                                                                <  2:826734/151‑1 (MQ=255)                                              gggAAACTAAAAGCCAACCTGATAAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATc                                                                                                >  2:535796/1‑150 (MQ=255)                                                  aaCTAAAAGCCAACCTGATAAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGa                                                                                           <  2:465463/151‑1 (MQ=255)                                                      aaaaGCCAACCTGATAAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAAt                                                                                         <  1:536834/149‑1 (MQ=255)                                                           ccAACCTGATAAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAgg                                                                                  <  1:244072/151‑1 (MQ=255)                                                               ccTGATAAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCtt                                                                              <  2:190374/151‑1 (MQ=255)                                                                     aaaTCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGtgat                                                                        >  2:718319/1‑151 (MQ=255)                                                                         cATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGAtt                                                                    <  1:516983/151‑1 (MQ=255)                                                                          aTAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTg                                                                   >  1:336310/1‑151 (MQ=255)                                                                                 gACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTat                                                            <  1:493172/151‑1 (MQ=255)                                                                                  aCTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTatc                                                           <  2:127614/151‑1 (MQ=255)                                                                                      aGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCg                                                       >  2:624609/1‑151 (MQ=255)                                                                                       ggAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGc                                                      <  2:779944/151‑1 (MQ=255)                                                                                        gAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCa                                                     <  1:814095/151‑1 (MQ=255)                                                                                               tGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCAttc                                              >  2:639331/1‑149 (MQ=255)                                                                                                    gAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTc                                         <  2:218128/151‑1 (MQ=255)                                                                                                     aaGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCt                                        >  2:572003/1‑151 (MQ=255)                                                                                                      aGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCtt                                       <  1:129820/151‑1 (MQ=255)                                                                                                         cGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTAc                                    >  2:279056/1‑151 (MQ=255)                                                                                                            aTTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTACCTg                                 <  1:781181/151‑1 (MQ=255)                                                                                                               gaggagCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTACCTGGac                              >  2:68782/1‑151 (MQ=255)                                                                                                                aggagCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTACCTGGacc                             >  2:41989/1‑151 (MQ=255)                                                                                                                   agCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTACCTGGaccacc                          <  1:78171/151‑1 (MQ=255)                                                                                                                         catcatGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTACCTGGACCACCGATgg                     <  2:645937/150‑1 (MQ=255)                                                                                                                              tGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTACCTGGACCACCGATGGTGCCt                >  2:82460/1‑150 (MQ=255)                                                                                                                               gTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTACCTGGACCCCCGATGGTGCCTa               <  2:370911/150‑1 (MQ=255)                                                                                                                                 cGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTACCTGGACCACCGATGGTGCCTACt             <  1:636463/150‑1 (MQ=255)                                                                                                                                   aaaaGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTACCTGGACCACCGATGGTGACTACTTa           >  2:458526/1‑150 (MQ=255)                                                                                                                                    aaaGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTACCTGGACCACCGATGGTGCCTACTTa           >  1:279799/1‑149 (MQ=255)                                                                                                                                     aaGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTACCTGGACCACCGATGGTGCCTACTTAAgc        >  1:753937/1‑151 (MQ=255)                                                                                                                                       gCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTACCTGGACCACCGATGGTGCCTACTTAAgcgc      >  1:255178/1‑151 (MQ=255)                                                                                                                                       gCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTACCTGGACCACCGATGGTGCCTACTTAAgcgc      <  1:718368/151‑1 (MQ=255)                                                                                                                                       gCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTACCTGGACCACCGATGGTGCCTACTTAAgcgc      >  1:566786/1‑151 (MQ=255)                                                                                                                                        cAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTACCTGGACCACCGATGGTGCCTACTTAAGCGcc     >  1:608344/1‑151 (MQ=255)                                                                                                                                         aaTCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTACCTGGACCACCGATGGTGCCTACTTAAGCGccc    >  1:164819/1‑151 (MQ=255)                                                                                                                                            ccGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTACCTGGACCACCGATGGTGCCTACTTAAGCGCCCGt  >  1:73876/1‑150 (MQ=255)                                                                                                                                               |                                                                                                                                                   CCTGAAGTGCTCGGCCTTAGCGATCGTGTACTGGTGATGCATGAAGGGAAACTAAAAGCCAACCTGATAAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTACCTGGACCACCGATGGTGCCTACTTAAGCGCCCGT  >  NC_000913/3733596‑3733884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑