Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,844,363	T→C	F134L (TTT→CTT)	adeD →	cryptic adenine deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,844,363	0	T	C	100.0%	196.6 / NA	54	F134L (TTT→CTT)	adeD	cryptic adenine deaminase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/31);  total (23/31)

GGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTATTTGCGACCCTCATGAAATCGTCAACGTGATGGGCGAAGCCGGATTCGCCTGGTTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGCCAGTTTTACCCTTGAACAGATGCTCGCCTGGCGGGACCATCCGCAGGTTA  >  NC_000913/3844216‑3844504                                                                                                                                                    |                                                                                                                                              gggTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTATTTGCGACCCTCATGAAATCGTCAACGTGATGGGCGAAGCCGGATTCGCCTGGCTTg                                                                                                                                            >  2:227970/1‑151 (MQ=255) gggTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTATTTGCGACCCTCATGAAATCGTCAACGTGATGGGCGAAGCCGGATTCGCCTGGCTTg                                                                                                                                            <  1:245947/151‑1 (MQ=255)  ggTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTATTTGCGACCCTCATGAAATCGTCAACGTGATGGGCGAAGCCGGATTCGCCTGGCTTGc                                                                                                                                           >  1:135799/1‑151 (MQ=255)         tGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTATTTGCGACCCTCATGAAATCGTCAACGTGATGGGCGAAGCCGGATTCGCCTGGCTTGCCCGCtg                                                                                                                                     >  2:268404/1‑150 (MQ=255)           aTGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTATTTGCGACCCTCATGAAATCGTCAACGTGATGGGCGAAGCCGGATTCGCCTGGCTTGCCCGCTGTGc                                                                                                                                  <  1:526305/151‑1 (MQ=255)                cACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTATTTGCGACCCTCATGAAATCGTCAACGTGATGGGCGAAGCCGGATTCGCCTGGCTTGCCCGCTGTGCCGa                                                                                                                               <  1:60544/149‑1 (MQ=255)                     gCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTATTTGCGACCCTCATGAAATCGTCAACGTGATGGGCGAAGCCGGATTCGCCTGGCTTGCCCGCTGTGCCGAACagg                                                                                                                          <  1:781401/149‑1 (MQ=255)                      cATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTATTTGCGACCCTCATGAAATCGTCAACGTGATGGGCGAAGCCGGATTCGCCTGGCTTGCCCGCTGTGCCGAACaggca                                                                                                                        >  2:889867/1‑150 (MQ=255)                      cATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTATTTGCGACCCTCATGAAATCGTCAACGTGATGGGCGAAGCCGGATTCGCCTGGCTTGCCCGCTGTGCCGAACaggca                                                                                                                        >  1:197239/1‑150 (MQ=255)                         atTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTATTTGCGACCCTCATGAAATCGTCAACGTGATGGGCGAAGCCGGATTCGCCTGGCTTGCCCGCTGTGCCGAACaggcaagg                                                                                                                     <  2:777265/150‑1 (MQ=255)                              aTCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTATTTGCGACCCTCATGAAATCGTCAACGTGATGGGCGAAGCCGGATTCGCCTGGCTTGCCCGCTGTGCCGAACAGGCAAGGCAAAAc                                                                                                               >  1:275294/1‑151 (MQ=255)                               tCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTATTTGCGACCCTCATGAAATCGTCAACGTGATGGGCGAAGCCGGATTCGCCTGGCTTGCCCGCTGTGCCGAACAGGCAAGGCAAAAcc                                                                                                              >  1:638536/1‑151 (MQ=255)                                 cAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTATTTGCGACCCTCATGAAATCGTCAACGTGATGGGCGAAGCCGGATTCGCCTGGCTTGCCCGCTGTGCCGAACAGGCAAGGCAAAAcc                                                                                                              >  2:359855/1‑149 (MQ=255)                                    cATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTATTTGCGACCCTCATGAAATCGTCAACGTGATGGGCGAAGCCGGATTCGCCTGGCTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTAc                                                                                                         <  2:381861/151‑1 (MQ=255)                                    cATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTATTTGCGACCCTCATGAAATCGTCAACGTGATGGGCGAAGCCGGATTCGCCTGGCTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTAc                                                                                                         >  1:143279/1‑151 (MQ=255)                                    cATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTATTTGCGACCCTCATGAAATCGTCAACGTGATGGGCGAAGCCGGATTCGCCTGGCTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTAc                                                                                                         <  2:33159/151‑1 (MQ=255)                                     atgatgACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTATTTGCGACCCTCATGAAATCGTCAACGTGATGGGCGAAGCCGGATTCGCCTGGCTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACt                                                                                                        >  1:653004/1‑151 (MQ=255)                                              ccGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTATTTGCGACCCTCATGAAATCGTCAACGTGATGGGCGAAGCCGGATTCGCCTGGCTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCa                                                                                               <  1:72284/151‑1 (MQ=255)                                                   cACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTATTTGCGACCCTCATGAAATCGTCAACGTGATGGGCGAAGCCGGATTCGCCTGGCTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGctct                                                                                          <  1:157475/151‑1 (MQ=255)                                                   cACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTATTTGCGACCCTCATGAAATCGTCAACGTGATGGGCGAAGCCGGATTCGCCTGGCTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGctct                                                                                          <  1:331741/151‑1 (MQ=255)                                                   cACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTATTTGCGACCCTCATGAAATCGTCAACGTGATGGGCGAAGCCGGATTCGCCTGGCTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGctc                                                                                           >  2:29187/1‑150 (MQ=255)                                                         tGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTATTTGCGACCCTCATGAAATCGTCAACGTGATGGGCGAAGCCGGATTCGCCTGGCTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGt                                                                                     >  2:303401/1‑150 (MQ=255)                                                            aaCCGCTACCCTGCCGCGCAGCCTGACGACCGTTATTTGCGACCCTCATGAAATCGTCAACGTGATGGGCGAAGCCGGATTCGCCTGGCTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTAccc                                                                                 <  1:712126/151‑1 (MQ=255)                                                              ccGCTACCCTGCCGCGCGGCCTGACGACCGTTATTTGCGACCCTCATGAAATCGTCAACGTGATGGGCGAAGCCGGATTCGCCTGGCTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGc                                                                               <  2:698823/151‑1 (MQ=255)                                                                   aCCCTGCCGCGCGGCCTGACGACCGTTATTTGCGACCCTCATGAAATCGTCAACGTGATGGGCGAAGCCGGATTCGCCTGGCTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTgg                                                                          >  2:187529/1‑151 (MQ=255)                                                                   aCCCTGCCGCGCGGCCTGACGACCGTTATTTGCGACCCTCATGAAATCGGCAACGTGATGGGCGAAGCCGGATTCGCCTGGCTTGCCCGCTGTGCCGAACAGGCAAGGCAAATCCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCt                                                                            <  2:879924/149‑1 (MQ=255)                                                                    cccTGCCGCGCGGCCTGACGACCGTTATTTGCGACCCTCATGAAATCGTCAACGTGATGGGCGAAGCCGGATTCGCCTGGCTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTgg                                                                          <  2:369529/150‑1 (MQ=255)                                                                         ccGCGCGGCCTGACGACCGTTATTTGCGACCCTCATGAAATCGTCAACGTGATGGGCGAAGCCGGATTCGCCTGGCTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCt                                                                    <  1:243305/151‑1 (MQ=255)                                                                            cgcgGCCTGACGACCGTTATTTGCGACCCTCATGAAATCGTCAACGTGATGGGCGAAGCCGGATTCGCCTGGCTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCg                                                                 <  1:865300/151‑1 (MQ=255)                                                                               ggCCTGACGACCGTTATTTGCGACCCTCATGAAATCGTCAACGTGATGGGCGAAGCCGGATTCGCCTGGCTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATg                                                              <  2:107214/151‑1 (MQ=255)                                                                                    gacgacCGTTATTTGCGACCCTCATGAAATCGTCAACGTGATGGGCGAAGCCGGATTCGCCTGGCTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAAc                                                         >  1:777827/1‑151 (MQ=255)                                                                                         ccGTTATTTGCGACCCTCATGAAATCGTCAACGTGATGGGCGAAGCCGGATTCGCCTGGCTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGc                                                    <  1:882561/151‑1 (MQ=255)                                                                                          cGTTATTTGCGACCCTCATGAAATCGTCAACGTGACGGGCGAAGCCGGATTCGCCTGGCTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTCCAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGcc                                                   <  2:463175/151‑1 (MQ=255)                                                                                             tATTTGCGACCCTCATGAAATCGTCAACGTGATGGGCGAAGCCGGATTCGCCTGGCTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGCCAg                                                 <  1:317179/150‑1 (MQ=255)                                                                                                ttGCGACCCTCATGAAATCGTCAACGTGATGGGCGAAGCCGGATTCGCCTGGCTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGCCAGttt                                              <  1:725226/150‑1 (MQ=255)                                                                                                  gCGACCCTCATGAAATCGTCAACGTGATGGGCGAAGCCGGATTCGCCTGGCTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGCCAGTTTTa                                            <  2:531356/150‑1 (MQ=255)                                                                                                      cccTCATGAAATCGTCAACGTGATGGGCGAAGCCGGATTCGCCTGGCTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGCCAGTTTTACCCtt                                       <  2:453551/151‑1 (MQ=255)                                                                                                       ccTCATGAAATCGTCAACGTGATGGGCGAAGCCGGATTCGCCTGGCTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGCCAGTTTTACCCTTg                                      <  1:352131/151‑1 (MQ=255)                                                                                                             gAAATCGTCAACGTGATGGGCGAAGCCGGATTCGCCTGGCTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGCCAGTTTTACCCTTGAAc                                   >  1:42697/1‑148 (MQ=255)                                                                                                             gAAATCGTCAACGTGATGGGCGAAGCCGGATTCGCCTGGCTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGCCAGTTTTACCCTTGAACAg                                 <  1:125744/150‑1 (MQ=255)                                                                                                              aaaTCGTCAACTTGATGGGCGAAGCCGGATTCGCCTGGCTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGCCAGTTTTGCCCTTGaa                                    <  2:427684/146‑1 (MQ=255)                                                                                                                  cGTCAACGTGATGGGCGAAGCCGGATTCGCCTGGCTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGCCAGTTTTACCCTTGAACAGATGCTc                           <  2:545026/151‑1 (MQ=255)                                                                                                                  cGTCAACGTGATGGGCGAAGCCGGATTCGCCTGGCTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGCCAGTTTTACCCTTGAACAGATGCTc                           >  1:379712/1‑151 (MQ=255)                                                                                                                   gTCAACGTGATGGGCGAAGCCGGATTCGCCTGGCTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGCCAGTTTTACCCTTGAACAGATGCTCg                          <  2:439040/151‑1 (MQ=255)                                                                                                                   gTCAACGTGATGGGCGAAGCCGGATTCGCCTGGCTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGCCAGTTTTACCCTTGAACAGATGCTCg                          >  2:257145/1‑151 (MQ=255)                                                                                                                     cAACGTGATGGGCGAAGCCGGATTCGCCTGGCTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGCCAGTTTTACCCTTGAACAGATGCTCGcc                        >  2:843484/1‑151 (MQ=255)                                                                                                                      aaCGTGATGGGCGAAGCCGGATTCGCCTGGCTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGCCAGTTTTACCCTTGAACAGATGCTCGCCt                       >  2:471531/1‑151 (MQ=255)                                                                                                                          tGATGGGCGAAGCCGGATTCGCCTGGCTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGCCAGTTTTACCCTTGAACAGATGCTCGCCTGGCg                   >  1:14009/1‑151 (MQ=255)                                                                                                                           gATGGGCGAAGCCGGATTCGCCTGGCTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGCCAGTTTTACCCTTGAACAGATGCTCGCCTGGc                    >  1:663477/1‑149 (MQ=255)                                                                                                                                gCGAAGCCGGATTCGCCTGGCTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGCCAGTTTTACCCTTGAACAGATGCTCGCCTGGCGGGACCa             <  1:616323/151‑1 (MQ=255)                                                                                                                                  gAAGCCGGATTCGCCTGGCTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGCCAGTTTTACCCTTGAACAGATGCTCGCCTGGCGGGACCATc           >  2:273487/1‑151 (MQ=255)                                                                                                                                    aGCCGGATTCGCCTGGCTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGCCAGTTTTACCCTTGAACAGATGCTCGCCTGGCGGGACCATcc          >  2:698212/1‑150 (MQ=255)                                                                                                                                    aGCCGGATTCGCCTGGCTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGCCAGTTTTACCCTTGAACAGATGCTCGCCTGGCGGGACCATCCg         <  2:857571/151‑1 (MQ=255)                                                                                                                                            tCGCCTGGCTTGCCCGCTGTGCCGAACAGGCAAGGAAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGCCAGTTTTGCCCTTGAACAGATGCTCGCCTGGCGGGACCATCCGCAGGTTa  <  2:570342/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                              GGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACCGTTATTTGCGACCCTCATGAAATCGTCAACGTGATGGGCGAAGCCGGATTCGCCTGGTTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGCCAGTTTTACCCTTGAACAGATGCTCGCCTGGCGGGACCATCCGCAGGTTA  >  NC_000913/3844216‑3844504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑