Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,906,128	A→G	V147A (GTG→GCG)	bglG ←	transcriptional antiterminator of the bgl operon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,906,128	0	A	G	100.0%	179.7 / NA	53	V147A (GTG→GCG)	bglG	transcriptional antiterminator of the bgl operon
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/24);  total (29/24)

ATTAAGGCTGAACTGAAATTTTATTAATTGCAGCATTTCGCGCATTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCACGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGGCAGCAACACGTTTTGCTGAAAGCGTTTAATCGCAAACTGGCAATGGTCA  >  NC_000913/3905986‑3906271                                                                                                                                               |                                                                                                                                                aTTAAGGCTGAACTGAAATTTTATTAATTGCAGCATTTCGCGCATTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAAc                                                                                                                                         >  1:736162/1‑151 (MQ=255)      ggCTGAACTGAAATTTTATTAATTGCAGCATTTCGCGCATTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGttt                                                                                                                                    >  1:861237/1‑151 (MQ=255)           aactgaaATTTTATTAATTGCAGCATTTCGCGCATTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCa                                                                                                                                >  2:363772/1‑150 (MQ=255)            actgaaATTTTATTAATTGCAGCATTTCGCGCATTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCaat                                                                                                                              <  1:360520/151‑1 (MQ=255)             ctgaaATTTTATTAATTGCAGCATTTCGCGCATTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCaat                                                                                                                              >  2:207747/1‑150 (MQ=255)             ctgaaATTTTATTAATTGCAGCATTTCGCGCATTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCaat                                                                                                                              >  2:748546/1‑150 (MQ=255)             ctgaaATTTTATTAATTGCAGCATTTCGCGCATTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCaat                                                                                                                              >  1:519134/1‑150 (MQ=255)               gaaATTTTATTAATTGCAGCATTTCGCGCATTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCaataat                                                                                                                           <  2:543314/151‑1 (MQ=255)               gaaATTTTATTAATTGCAGCATTTCGCGCATTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCaataa                                                                                                                            <  2:463920/150‑1 (MQ=255)                aaaTTTTATTAATTGCAGCATTTCGCGCATTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATg                                                                                                                          <  1:258410/151‑1 (MQ=255)                   ttttATTAATTGCAGCATTTCGCGCATTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGtt                                                                                                                       >  2:133984/1‑151 (MQ=255)                      tattaATTGCAGCATTTCGCGCATTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAAc                                                                                                                    >  1:563269/1‑151 (MQ=255)                       attaATTGCAGCATTTCGCGCATTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAAc                                                                                                                    <  2:112037/150‑1 (MQ=255)                       attaATTGCAGCATTTCGCGCATTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACg                                                                                                                   >  2:843639/1‑151 (MQ=255)                          aaTTGCAGCATTTCGCGCATTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGctt                                                                                                                <  2:380413/151‑1 (MQ=255)                            ttGCAGCATTTCGCGCATTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGcttct                                                                                                              >  1:515439/1‑151 (MQ=255)                              gcagcaTCTCGCGCATTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTTACCACATGCATGGCAATAAAGCCCGCTTCATTTTTCTGTAACTGCGCGCCCCAGCGTTCATCAATAATGGTTAACActgctt                                                                                                             <  2:598932/150‑1 (MQ=255)                                          gcATTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGa                                                                                                 >  2:620737/1‑150 (MQ=255)                                                   gCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCgg                                                                                       <  1:341612/151‑1 (MQ=255)                                                            cTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCg                                                                              >  2:515143/1‑151 (MQ=255)                                                              gCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGc                                                                             <  2:694555/150‑1 (MQ=255)                                                                aaCATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTgg                                                                          >  2:716925/1‑151 (MQ=255)                                                                 aCATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGa                                                                         <  2:902650/151‑1 (MQ=255)                                                                         cATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCcac                                                                 >  1:800486/1‑151 (MQ=255)                                                                            atTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGc                                                              >  1:399199/1‑151 (MQ=255)                                                                            atTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGc                                                              >  2:459577/1‑151 (MQ=255)                                                                                ccGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAAc                                                           <  2:29781/150‑1 (MQ=255)                                                                                ccGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACg                                                          <  2:756604/151‑1 (MQ=255)                                                                                 cGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACgg                                                         <  2:267175/151‑1 (MQ=255)                                                                                  gCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACggg                                                        <  1:321137/151‑1 (MQ=255)                                                                                     cATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTg                                                     >  1:588056/1‑151 (MQ=255)                                                                                     cATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTg                                                     <  2:451935/151‑1 (MQ=255)                                                                                         tGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGgc                                                  >  1:760454/1‑150 (MQ=255)                                                                                            gCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGgcagc                                               >  1:742932/1‑150 (MQ=255)                                                                                             cACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGgcagca                                              >  1:215731/1‑150 (MQ=255)                                                                                                 gACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGGCAGCAACACg                                         <  2:71314/151‑1 (MQ=255)                                                                                                  aCCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGGCAGCAACACGt                                        <  2:596026/151‑1 (MQ=255)                                                                                                   ccAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGGCAGCAACACGtt                                       <  2:774045/151‑1 (MQ=255)                                                                                                    cAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGGCAGCAACACGttt                                      <  2:61158/151‑1 (MQ=255)                                                                                                     aGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGGCAGCAACACGtttt                                     >  2:826284/1‑151 (MQ=255)                                                                                                           aTGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGGCAGCAACACGTTTTGCt                                  <  2:254062/148‑1 (MQ=255)                                                                                                                aaTAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGGCAGCAACACGTTTTGCTGAAAGCGt                          >  2:234553/1‑151 (MQ=255)                                                                                                                  tAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTTACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGGCAGCAACACGTTTTGCTGAAAGCGttt                        >  2:568387/1‑151 (MQ=255)                                                                                                                  tAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGGCAGCAACACGTTTTGCTGAAAGCGttt                        >  2:890769/1‑151 (MQ=255)                                                                                                                   aaaGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGGCAGCAACACGTTTTGCTGAAAGCGttt                        <  1:427162/150‑1 (MQ=255)                                                                                                                    aaGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGGCAGCAACACGTTTTGCTGAAAGCGTTTaa                      <  1:826351/151‑1 (MQ=255)                                                                                                                        ccACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGGCAGCAACACGTTTTGCTGAAAGCGTTTAATCGc                  >  2:399430/1‑151 (MQ=255)                                                                                                                             tCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGGCAGCAACACGTTTTGCTGAAAGCGTTTAATCGCAAACt             >  2:831852/1‑151 (MQ=255)                                                                                                                              cATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGGCAGCAACACGTTTTGCTGAAAGCGTTTAATCGCAAACt             <  2:588007/150‑1 (MQ=255)                                                                                                                                tCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGGCAGCAACACGTTTTGCTGAAAGCGTTTAATCGCAAACTGGc          >  1:647275/1‑151 (MQ=255)                                                                                                                                 cTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGGCAGCAACACGTTTTGCTGAAAGCGTTTAATCGCAAACTGGc          >  2:289429/1‑150 (MQ=255)                                                                                                                                    tCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGGCAGCAACACGTTTTGCTGAAAGCGTTTAATCGCAAACTGGCAATg      >  2:602317/1‑151 (MQ=255)                                                                                                                                         aaCTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGGCAGCAACACGTTTTGCTGAAAGCGTTTAATCGCAAACTGGCAATGGTCa  <  1:478949/150‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                ATTAAGGCTGAACTGAAATTTTATTAATTGCAGCATTTCGCGCATTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCACGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGGCAGCAACACGTTTTGCTGAAAGCGTTTAATCGCAAACTGGCAATGGTCA  >  NC_000913/3905986‑3906271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑