Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	471,467	A→G	T278A (ACC→GCC)	mdlB →	putative multidrug ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	471,467	0	A	G	100.0%	187.2 / NA	54	T278A (ACC→GCC)	mdlB	putative multidrug ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/24);  total (30/24)

ACGTATGGGGGAGGCCAGTCGTTCACACTATATGGCGAGGATGCAAACCCTGCGCCTCGACGGTTTTCTGCTGCGTCCGCTGCTGAGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGC  >  NC_000913/471319‑471613                                                                                                                                                     |                                                                                                                                                   aCGTATGGGGGAGGCCAGTCGTTCACACTATATGGCGAGGATGCAAACCCTGCGCCTCGACGGTTTTCTGCTGCGTCCGCTGCTGAGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCGcc                                                                                                                                                  >  1:377990/1‑151 (MQ=255)     aTGGGGGAGGCCAGTCGTTCACACTATATGGCGAGGATGCAAACCCTGCGCCTCGACGGTTTTCTGCTGCGTCCGCTGCTGAGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCGCCATTg                                                                                                                                              >  1:881238/1‑151 (MQ=255)       gggggAGGCCAGTCGTTCACACTATATGGCGAGGATGCAACCCCTGCGCCTCGACGGTTTTCTGCTGCGTCCGCTGCTGAGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGAGCCATTGaa                                                                                                                                            <  2:889194/151‑1 (MQ=255)       gggggAGGCCAGTCGTTCACACTATATGGCGAGGATGCAAACCCTGCGCCTCGACGGTTTTCTGCTGCGTCCGCTGCTGAGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCGCCATTGaa                                                                                                                                            <  1:640153/151‑1 (MQ=255)        ggggAGGCCAGTCGTTCACACTATATGGCGAGGATGCAAACCCTGCGCCTCGACGGTTTTCTGCTGCGTCCGCTGCTGAGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTTTCCGCCAGCCGCGCCCtt                                                                                                                                               >  2:146836/1‑147 (MQ=255)         gggAGGCCAGTCGTTCACACTATATGGCGAGGATGCAAACCCTGCGCCTCGACGGTTTTCTGCTGCGTCCGCTGCTGAGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCGCCATTGAAGt                                                                                                                                          <  1:436766/151‑1 (MQ=255)          ggAGGCCAGTCGTTCACACTATATGGCGAGGATGCAAACCCTGCGCCTCGACGGTTTTCTGCTGCGTCCGCTGCTGAGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCGCCATTGAAGTg                                                                                                                                         <  2:681815/151‑1 (MQ=255)            aGGCCAGTCGTTCACACTATATGGCGAGGATGCAAACCCTGCGCCTCGACGGTTTTCTGCTGCGTCCGCTGCTGAGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCGCCATTGAAGTggg                                                                                                                                       >  1:444775/1‑151 (MQ=255)             ggCCAGTCGTTCACACTATATGGCGAGGATGCAAACCCTGCGCCTCGACGGTTTTCTGCTGCGTCCGCTGCTGAGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCGCCATTGAAGTggg                                                                                                                                       >  2:377325/1‑150 (MQ=255)               ccAGTCGTTCACACTATATGGCGAGGATGCAAACCCTGCGCCTCGACGGTTTTCTGCTGCGTCCGCTGCTGAGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCGCCATTGAAGTGGGCGt                                                                                                                                    <  2:756464/151‑1 (MQ=255)                cAGTCGTTCACACTATATGGCGAGGATGCAAACCCTGCGCCTCGACGGTTTTCTGCTGCGTCCGCTGCTGAGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCGCCATTGAAGTGGGCGTg                                                                                                                                   <  2:217660/151‑1 (MQ=255)                 aGTCGTTCACACTATATGGCGAGGATGCAAACCCTGCGCCTCGACGGTTTTCTGCTGCGTCCGCTGCTGAGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCGCCATTGAAGTGGGCGTGc                                                                                                                                  >  1:429293/1‑151 (MQ=255)                    cGTTCACACTATATGGCGAGGATGCAAACCCTGCGCCTCGACGGTTTTCTGCTGCGTCCGCTGCTGAGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCGCCATTGAAGTGGGCGTGCTg                                                                                                                                <  1:544515/150‑1 (MQ=255)                         acacTATATGGCGAGGATGCAAACCCTGCGCCTCGACGGTTTTCTGCTGCGTCCGCTGCTGAGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCGCCATTGAAGTGGGCGTGCTGTATGCg                                                                                                                          >  2:667021/1‑151 (MQ=255)                           acTATATGGCGAGGATGCAAACCCTGCGCCTCGACGGTTTTCTGCTGCGTCCGCTGCTGAGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCGCCATTGAAGTGGGCGTGCTGTATGCGtt                                                                                                                        >  2:698801/1‑151 (MQ=255)                              atatGGCGAGGATGCAAACCCTGCGCCTCGACGGTTTTCTGCTGCGTCCGCTGCTGAGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCGCCATTGAAGTGGGCGTGCTGTATGCGttt                                                                                                                       <  1:731473/149‑1 (MQ=255)                              atatGGCGAGGATGCAAACCCTGCGCCTCGACGGTTTTCTGCTGCGTCCGCTGCTGAGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCGCCATTGAAGTGGGCGTGCTGTATGCGTTTAt                                                                                                                     <  2:492709/151‑1 (MQ=255)                                atGGCGAGGATGCAAACCCTGCGCCTCGACGGTTTTCTGCTGCGTCCGCTGCTGAGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCGCCATTGAAGTGGGCGTGCTGTATGCGTTTATc                                                                                                                    <  1:801756/150‑1 (MQ=255)                                 tGGCGAGGATGCAAACCCTGCGCCTCGACGGTTTTCTGCTGCGTCCGCTGCTGAGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCGCCATTGAAGTGGGCGTGCTGTATGCGTTTATc                                                                                                                    >  1:158904/1‑149 (MQ=255)                                  ggCGAGGATGCAAACCCTGCGCCTCGACGGTTTTCTGCTGCGTCCGCTGCTGAGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCGCCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGc                                                                                                                 >  1:540631/1‑151 (MQ=255)                                                   tGCGCCTCGACGGTTTTCTGCTGCGTCCGCTGCTGAGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCGCCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTa                                                                                                 >  1:774847/1‑150 (MQ=255)                                                    gcgcCTCGGCGGTTTTCTGCTGCGTCCGCTGCTGAGTCTGTTTTCAGCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCGCCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAAc                                                                                               <  2:621951/151‑1 (MQ=255)                                                    gcgcCTCGACGGTTTTCTGCTGCGTCCGCTGCTGAGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCGCCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTaa                                                                                                <  1:544163/150‑1 (MQ=255)                                                          cGACGGTTTTCTGCTGCGTCCGCTGCTGAGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCGCCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAAcc                                                                                          >  2:692603/1‑150 (MQ=255)                                                             cGGTTTTCTGCTGCGTCCGCTGCTGAGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTTATGCTGTTTGGCTTCTCCGCCAGCGGCGCCATTTAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCAtt                                                                                       >  1:81514/1‑150 (MQ=255)                                                                 tttCTGCTGCGTCCGCTGCTGAGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCGCCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATc                                                                                   <  2:53217/150‑1 (MQ=255)                                                                   tCTGCTGCGTCCGCTGCTGAGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCGCCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGa                                                                                 >  1:3071/1‑150 (MQ=255)                                                                     tgctgcGTCCGCTGCTGAGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCGCCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACt                                                                              >  2:697538/1‑151 (MQ=255)                                                                      gctgcGTCCGCTGCTGAGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCGCCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGaa                                                                                <  1:908042/148‑1 (MQ=255)                                                                       ctgcGTCCGCTGCTGAGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCGCCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTg                                                                             >  1:698127/1‑150 (MQ=255)                                                                             ccGCTGCTGAGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCGCCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACg                                                                       <  2:834950/150‑1 (MQ=255)                                                                                ctgctgAGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCGCCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCaa                                                                    >  2:590093/1‑150 (MQ=255)                                                                                  gctgAGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCGCCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAAc                                                                   >  1:429023/1‑149 (MQ=255)                                                                                      aGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCGCCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCg                                                              >  1:437026/1‑150 (MQ=255)                                                                                       gTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCGCCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGAt                                                            <  2:155489/151‑1 (MQ=255)                                                                                               tCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCGCCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCaa                                                     >  2:119383/1‑150 (MQ=255)                                                                                                   cGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCGCCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAgg                                                 <  1:698294/150‑1 (MQ=255)                                                                                                        aTTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCGCCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCtgttg                                           >  1:636576/1‑151 (MQ=255)                                                                                                             ttGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCGCCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTgttgtt                                         <  1:698850/148‑1 (MQ=255)                                                                                                               gtgGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCGCCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTgg                                    >  1:76941/1‑151 (MQ=255)                                                                                                                tgGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCGCCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGt                                   >  1:697522/1‑151 (MQ=255)                                                                                                                tgGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCGCCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGt                                   >  1:440195/1‑151 (MQ=255)                                                                                                                     tgttgATGCTGTTTGGCTTCTCCGCCAGCGGCGCCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAgc                               <  1:894997/150‑1 (MQ=255)                                                                                                                        tgATGCTGTTTGGCTTCTCCGCCAGCGGCGCCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGcgcg                            <  1:805288/150‑1 (MQ=255)                                                                                                                            gCTGTTTGGCTTCTCCGCCAGCGGCGCCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGttt                       >  1:879382/1‑151 (MQ=255)                                                                                                                             cTGTTTGGCTTCTCCGCCAGCGGCGCCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTg                      >  2:848840/1‑151 (MQ=255)                                                                                                                                tttGGCTTCTCCGCCAGCGGCGCCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAAc                   >  1:749452/1‑151 (MQ=255)                                                                                                                                    gCTTCTCCGCCAGCGGCGCCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGAt               <  1:585606/151‑1 (MQ=255)                                                                                                                                       tctcCGCCAGCGGCGCCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATg              <  1:826869/149‑1 (MQ=255)                                                                                                                                          ccGCCAGCGGCGCCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATggacg          <  1:142181/150‑1 (MQ=255)                                                                                                                                          ccGCCAGCGGCGCCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATggacg          <  1:519782/150‑1 (MQ=255)                                                                                                                                              cAGCGGCGCCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACcg     >  1:232217/1‑151 (MQ=255)                                                                                                                                               aGCGGCGCCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACcgc    >  1:236219/1‑151 (MQ=255)                                                                                                                                                gcggcgCCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACcgcg   <  2:646375/151‑1 (MQ=255)                                                                                                                                                 cGGCGCCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCgcgc  >  2:342918/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                   ACGTATGGGGGAGGCCAGTCGTTCACACTATATGGCGAGGATGCAAACCCTGCGCCTCGACGGTTTTCTGCTGCGTCCGCTGCTGAGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGC  >  NC_000913/471319‑471613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑