Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,930,513	T→C	Q194R (CAG→CGG)	ravA ←	hexameric AAA+ MoxR family ATPase, putative molecular chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,930,513	0	T	C	100.0%	178.0 / NA	49	Q194R (CAG→CGG)	ravA	hexameric AAA+ MoxR family ATPase, putative molecular chaperone
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/22);  total (27/22)

GGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCTGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGCAGCTCGTTGGAGGCCGCCACCAGCAGACGCATC  >  NC_000913/3930366‑3930656                                                                                                                                                    |                                                                                                                                                ggTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGtt                                                                                                                                              <  2:212773/151‑1 (MQ=255)  gTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTg                                                                                                                                             >  1:130110/1‑151 (MQ=255)    aaTTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGa                                                                                                                                            <  1:482209/150‑1 (MQ=255)    aaTTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGAc                                                                                                                                           >  1:764637/1‑151 (MQ=255)     aTTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACt                                                                                                                                          <  2:756447/151‑1 (MQ=255)         aTCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGt                                                                                                                                       <  2:391694/150‑1 (MQ=255)           ccAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCa                                                                                                                                     <  1:574678/150‑1 (MQ=255)                 gCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGa                                                                                                                              <  2:466935/151‑1 (MQ=255)                 gCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGa                                                                                                                              <  2:194031/151‑1 (MQ=255)                   tcgcgcAGCATAAAAATCAGCTCAAATACATGATCGGCCAGTGTAATTTCACCCATCTCTTTCTGCCAGTGTTCATATTCTACATCTGTGACCTGCAAGGCATCAGGAACGGGTTTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGa                                                                                                                              >  2:10396/3‑149 (MQ=255)                     gcgcAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCgg                                                                                                                          <  1:9806/151‑1 (MQ=255)                           caTAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTc                                                                                                                    >  2:716565/1‑151 (MQ=255)                                  aTCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTAt                                                                                                             >  2:372563/1‑151 (MQ=255)                                         cAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCAc                                                                                                      >  2:703320/1‑151 (MQ=255)                                          aaaTACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACt                                                                                                     >  1:37452/1‑151 (MQ=255)                                          aaaTACATGATCGGGCAGCGAAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCAGTGACCTGCAAGGCATCAGAAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACt                                                                                                     >  2:43492/1‑151 (MQ=255)                                           aaTACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACtt                                                                                                    <  2:38417/151‑1 (MQ=255)                                            aTACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACttt                                                                                                   >  2:484215/1‑151 (MQ=255)                                              aCATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTAt                                                                                                 >  2:147851/1‑151 (MQ=255)                                               cATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATc                                                                                                >  1:314013/1‑151 (MQ=255)                                                aTGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCt                                                                                               <  1:858537/151‑1 (MQ=255)                                                  gATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCAACTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCAACCCGTTGACTGATCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTAATCTa                                                                                              <  2:858603/150‑1 (MQ=255)                                                   aTCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAAc                                                                                            >  1:279058/1‑151 (MQ=255)                                                   aTCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAAc                                                                                            >  1:720279/1‑151 (MQ=255)                                                          aGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGAc                                                                                     <  1:281714/151‑1 (MQ=255)                                                                    cACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCAt                                                                           >  1:50809/1‑151 (MQ=255)                                                                     aCCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATg                                                                          >  2:667689/1‑151 (MQ=255)                                                                       cAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCg                                                                        <  1:628742/151‑1 (MQ=255)                                                                         aTCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGt                                                                      >  1:862452/1‑151 (MQ=255)                                                                          tctcTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTc                                                                     >  1:633720/1‑151 (MQ=255)                                                                               ttCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCata                                                                  <  2:409672/149‑1 (MQ=255)                                                                                 cTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAAc                                                              >  2:876462/1‑151 (MQ=255)                                                                                  tGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACg                                                             >  1:229634/1‑151 (MQ=255)                                                                                   gCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAAc                                                              <  1:753135/149‑1 (MQ=255)                                                                                           tCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGAc                                                    >  2:209977/1‑151 (MQ=255)                                                                                                 tcttcATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGActgctg                                               <  2:879468/150‑1 (MQ=255)                                                                                                 tcttcATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGActgctg                                               <  1:524915/150‑1 (MQ=255)                                                                                                     cATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGc                                          <  1:103112/151‑1 (MQ=255)                                                                                                      aTCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCATAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCt                                         >  1:9667/1‑151 (MQ=255)                                                                                                       tCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCtt                                        >  2:635917/1‑151 (MQ=255)                                                                                                                 gCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGCAGCt                               <  2:857316/150‑1 (MQ=255)                                                                                                                      gCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGCAGCTCGTTgg                         <  1:876533/151‑1 (MQ=255)                                                                                                                          cAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGCAGCTCGTTGGAGgc                     >  2:722084/1‑151 (MQ=255)                                                                                                                              aaCCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGCAGCTCGTTGGAGgccgcc                 >  1:70949/1‑151 (MQ=255)                                                                                                                                ccGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGCAGCTCGTTGGAGGCCGccac               >  2:88128/1‑151 (MQ=255)                                                                                                                                  ggATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGCAGCTCGTTGGAGGCCGccacc              >  1:828966/1‑150 (MQ=255)                                                                                                                                       gTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGCAGCTCGTTGGAGGCCGCCACcagcag         <  1:815133/150‑1 (MQ=255)                                                                                                                                         cGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGCAGCTCGTTGGAGGCCGCCACCAGCAGAc       <  2:512754/150‑1 (MQ=255)                                                                                                                                             ttCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGCAGCTCGTTGGAGGCCGCCACCAGCAGACGCATc  >  1:615901/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                GGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCTGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGCAGCTCGTTGGAGGCCGCCACCAGCAGACGCATC  >  NC_000913/3930366‑3930656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑