Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,967,714	C→T	intergenic (+38/‑202)	rho → / → wecA	transcription termination factor/UDP‑GlcNAc:undecaprenylphosphate GlcNAc‑1‑phosphate transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,967,714	0	C	T	100.0%	173.8 / NA	46	intergenic (+38/‑202)	rho/wecA	transcription termination factor/UDP‑GlcNAc:undecaprenylphosphate GlcNAc‑1‑phosphate transferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/18);  total (28/18)

CATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGCGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAATTTGCATATCAAATGGTTAATTTTTGCACAGGACTGGTGGGTTTGGAACGGACTTTCCCTTCTG  >  NC_000913/3967574‑3967859                                                                                                                                             |                                                                                                                                                  cATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCttt                                                                                                                                         >  2:135186/1‑151 (MQ=255)     cACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTtat                                                                                                                                      >  1:696911/1‑150 (MQ=255)         cGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTg                                                                                                                                 >  2:460609/1‑151 (MQ=255)             gggCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAAt                                                                                                                             <  1:76215/151‑1 (MQ=255)             gggCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAAt                                                                                                                             >  2:549492/1‑151 (MQ=255)                cGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCtt                                                                                                                          <  1:367053/151‑1 (MQ=255)                    aTCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATg                                                                                                                      >  2:786788/1‑151 (MQ=255)                        aTGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCcgc                                                                                                                  >  2:398885/1‑151 (MQ=255)                          gCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCgcgc                                                                                                                >  2:458049/1‑151 (MQ=255)                                gAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGa                                                                                                          <  2:415104/151‑1 (MQ=255)                                   ttCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGt                                                                                                       >  2:209285/1‑151 (MQ=255)                                        cATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAgg                                                                                                   >  1:525530/1‑150 (MQ=255)                                             ataaACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAAtt                                                                                             >  2:296363/1‑151 (MQ=255)                                             ataaACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAAtt                                                                                             >  2:801450/1‑151 (MQ=255)                                               aaaCTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTc                                                                                            <  1:909041/150‑1 (MQ=255)                                                     gCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAAt                                                                                     <  2:507511/151‑1 (MQ=255)                                                     gCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAAt                                                                                     <  2:846564/151‑1 (MQ=255)                                                        aTGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTg                                                                                  >  2:537217/1‑151 (MQ=255)                                                                  ccaaTGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGtt                                                                        >  2:412091/1‑151 (MQ=255)                                                                   caaTGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTa                                                                       <  2:78173/151‑1 (MQ=255)                                                                          gATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAAtt                                                                >  1:642224/1‑151 (MQ=255)                                                                                 tcGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAATTTGCat                                                           <  1:426829/149‑1 (MQ=255)                                                                                         atgaAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAATTTGCATATCAAATGGt                                                 >  2:48575/1‑151 (MQ=255)                                                                                          tgaAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAATTTGCATATCAAATGGtt                                                >  1:194941/1‑151 (MQ=255)                                                                                           gaAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAATTTGCATATCAAATGGtt                                                <  2:99079/150‑1 (MQ=255)                                                                                             aaCGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAATTTGCATATCAAATGGTTAAt                                             <  2:194916/151‑1 (MQ=255)                                                                                              aCGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAATTTGCATATCAAATGGTTAAtt                                            <  1:577192/151‑1 (MQ=255)                                                                                              aCGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAATTTGCATATCAAATGGTTAAtt                                            <  1:587600/151‑1 (MQ=255)                                                                                                  tCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAATTTGCATATCAAATGGTTAATTTTTg                                        >  1:362472/1‑151 (MQ=255)                                                                                                   cATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAATTTGCATATCAAATGGTTAAttttt                                         <  2:570137/149‑1 (MQ=255)                                                                                                   cATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAATTTGCATATCAAATGGTTAATTTTTGc                                       <  2:243431/151‑1 (MQ=255)                                                                                                    aTAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGTTGGCATGTTATGCAATTTGCATATCAAATGGTTAATTTTTGc                                       >  2:398572/1‑150 (MQ=255)                                                                                                    aTAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAATTTGCATATCAAATGGTTAATTTTTGc                                       >  1:460234/1‑150 (MQ=255)                                                                                                     tAAATTTGTCTTATGCCAAAAACGCCACCTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCTCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAATTTGCATATCAAATGGTTAATTTTTGca                                      <  2:317953/150‑1 (MQ=255)                                                                                                      aaaTTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAATTTGCATATCAAATGGTTAATTTTTGcac                                     >  1:458142/1‑150 (MQ=255)                                                                                                        aTTTGTCTTATGCCAAAAACGCCTCGTGTTTACGTTGTGTTTTTCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGTAATTTGCTGTCATGTTATGCAATTTGCATATCAAATGGTTAATTTTTGCACAgg                                  <  1:666873/151‑1 (MQ=255)                                                                                                         tttGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGCTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAATTTGCATATCAAATGGTTAATTTTTGCACAgg                                  >  2:713939/1‑150 (MQ=255)                                                                                                          ttGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAATTTGCATATCAAATGGTTAATTTTTGCACAGGAc                                >  1:68294/1‑151 (MQ=255)                                                                                                          ttGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAATTTGCATATCAAATGGTTAATTTTTGCACAGGAc                                >  1:24378/1‑151 (MQ=255)                                                                                                                tATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAATTTGCATATCAAATGGTTAATTTTTGCACAGGACtggtgg                          >  1:311603/1‑151 (MQ=255)                                                                                                                 aTGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAATTTGCATATCAAATGGTTAATTTTTGCACAGGACtggtgg                          <  1:488742/150‑1 (MQ=255)                                                                                                                  tGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAATTTGCATATCAAATGGTTAATTTTTGCACAGGACTGGTGGGt                        >  2:662121/1‑151 (MQ=255)                                                                                                                   gCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAATTTGCATATCAAATGGTTAATTTTTGCACAGGACTGGTGGGtt                       <  1:412118/151‑1 (MQ=255)                                                                                                                     cAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAATTTGCATATCAAATGGTTAATTTTTGCACAGGACTGGCGGGTTTg                     >  2:368030/1‑151 (MQ=255)                                                                                                                                cGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAATTTGCATATCAAATGGTTAATTTTTGCACAGGACTGGGGGGTTTGGAACGGACttt          >  2:335572/1‑151 (MQ=255)                                                                                                                                   gtTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAATTTGCATATCAAATGGTTAATTTTTGCACAGGACTGGTGGGTTTGGAACGGACTTTccc       >  1:294751/1‑151 (MQ=255)                                                                                                                                         gtggtgTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAATTTGCATATCAAATGGTTAATTTTTGCACAGGACTGGTGGGTTTGGAACGGACTTTCCCTTCTg  >  1:314928/1‑150 (MQ=255)                                                                                                                                             |                                                                                                                                                  CATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGCGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAATTTGCATATCAAATGGTTAATTTTTGCACAGGACTGGTGGGTTTGGAACGGACTTTCCCTTCTG  >  NC_000913/3967574‑3967859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑