Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,991,678	T→C	S176P (TCC→CCC)	cyaA →	adenylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,991,678	0	T	C	97.6%	138.1 / ‑6.7	42	S176P (TCC→CCC)	cyaA	adenylate cyclase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (24/17);  total (24/18)
Fisher's exact test for biased strand distribution p-value = 4.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

TAGCGAAGAGCGCCAATTGCTACAACGTAAATGTAGCCTGCTGGAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGACTATGTGATGACGCTTTACGCGCAGGGCGT  >  NC_000913/3991530‑3991820                                                                                                                                                     |                                                                                                                                               tAGCGAAGAGCGCCAATTGCTACAACGTAAATGTAGCCTGCTGGAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGcccc                                                                                                                                              >  2:355923/1‑151 (MQ=255)            gcCAATTGCTACAACGTAAATGTAGCCTGCTGGAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCatat                                                                                                                                   >  1:762426/1‑151 (MQ=255)              cAATTGCTACAACGTAAATGTAGCCTGCTGGAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATAc                                                                                                                                 <  2:834615/151‑1 (MQ=255)              cAATTGCTACAACGTAAATGTAGCCTGCTGGAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATAc                                                                                                                                 >  1:702278/1‑151 (MQ=255)                          cGTAAATGTAGCCTGCTGGAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACg                                                                                                                     <  1:113947/151‑1 (MQ=255)                          cGTAAATGTAGCCTGCTGGAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACg                                                                                                                     >  1:480065/1‑151 (MQ=255)                             aaaTGTAGTCTGCTGGAAATCTGGGCCGCCTCGCTGGGTGTGTAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATTAAAGCGACAGCATGGGTGGCGAAGTTTGGGGCTCCATCCAGCATATACTGCTGCTTGACGAAt                                                                                                                  <  2:780916/151‑1 (MQ=255)                              aaTGTAGCCTGCTGGAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAAtt                                                                                                                 >  1:39648/1‑151 (MQ=255)                                 gTAGCCTGCTGGAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAAtttt                                                                                                               <  1:452647/150‑1 (MQ=255)                                      ctgctgGAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGt                                                                                                          >  1:655908/1‑150 (MQ=255)                                        gctgGAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTAcc                                                                                                       >  2:92859/1‑151 (MQ=255)                                           ggAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTAccgcc                                                                                                    >  2:693822/1‑151 (MQ=255)                                              aaaCTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTg                                                                                                 >  2:156780/1‑151 (MQ=255)                                               aaCTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGc                                                                                                >  1:286677/1‑151 (MQ=255)                                                  tGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGtc                                                                                             >  1:107327/1‑151 (MQ=255)                                                    ggCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGtctc                                                                                           >  1:902394/1‑151 (MQ=255)                                                      ccgccTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGc                                                                                         >  2:371043/1‑151 (MQ=255)                                                       cgccTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGcc                                                                                        >  2:600083/1‑151 (MQ=255)                                                          cTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGt                                                                                     >  1:332848/1‑151 (MQ=255)                                                            cGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGt                                                                                     <  2:244811/149‑1 (MQ=255)                                                            cGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTaa                                                                                   <  2:199162/151‑1 (MQ=255)                                                              cTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGc                                                                                 >  2:811533/1‑151 (MQ=255)                                                                gggTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCg                                                                                <  2:100812/150‑1 (MQ=255)                                                                            cAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGaa                                                                    <  1:136412/150‑1 (MQ=255)                                                                             aGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAat                                                                   >  1:38572/1‑150 (MQ=255)                                                                                 tcttcCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATgg                                                               >  2:500719/1‑150 (MQ=255)                                                                                    tcCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGt                                                              <  1:279280/148‑1 (MQ=255)                                                                                    tcCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCACACCCAGCATATTCTGCTGCTTGAGGAATTTTTTCGTACCGCCGTGCGTCTCTCCGGTAAGCGTATTCTGTTGAATATGGTg                                                             <  2:610561/149‑1 (MQ=255)                                                                                        gattgatGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTg                                                        >  2:32893/1‑150 (MQ=255)                                                                                             atgaAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGcgacg                                                   >  2:127795/1‑150 (MQ=255)                                                                                                   aCCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGaagaag                                             <  1:371069/150‑1 (MQ=255)                                                                                                   aCCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAagaaga                                            <  2:619661/151‑1 (MQ=255)                                                                                                    ccGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGaagaag                                             >  1:667134/1‑149 (MQ=255)                                                                                                       cTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCAt                                        >  2:461028/1‑151 (MQ=255)                                                                                                         tCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGTGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCAt                                        <  1:457243/149‑1 (MQ=255)                                                                                                         tCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGACCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTTCCGTGCGCCGAAGAAGAGCAt                                        <  2:710810/149‑1 (MQ=255)                                                                                                           cGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTacg                                    <  2:742569/151‑1 (MQ=255)                                                                                                           cGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTacg                                    >  2:617485/1‑151 (MQ=255)                                                                                                                                  ggggggCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGACTATGTGATGACGCTTTAc             <  1:295119/151‑1 (MQ=255)                                                                                                                                          aaGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGACTATGTGATGACGCTTTACGCGCAggg     >  2:446783/1‑151 (MQ=255)                                                                                                                                           aGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGACTATGTGATGACGCTTTACGCGCAGGGc    >  2:166475/1‑151 (MQ=255)                                                                                                                                            gATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGACTATGTGATGACGCTTTACGCGCAGGGCg   <  1:461075/151‑1 (MQ=255)                                                                                                                                             aTTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGACTATGTGATGACGCTTTACGCGCAGGGCGt  <  2:902321/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                               TAGCGAAGAGCGCCAATTGCTACAACGTAAATGTAGCCTGCTGGAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGACTATGTGATGACGCTTTACGCGCAGGGCGT  >  NC_000913/3991530‑3991820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑