Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,009,349	C→T	C60C (TGC→TGT)	pldB →	lysophospholipase L2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,009,349	0	C	T	100.0%	194.8 / NA	50	C60C (TGC→TGT)	pldB	lysophospholipase L2
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/20);  total (30/20)

AAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGCCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATC  >  NC_000913/4009204‑4009494                                                                                                                                                  |                                                                                                                                                  aaaaCGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGTCCggg                                                                                                                                              >  1:830410/1‑151 (MQ=255)   aaCGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGTCCGGGGc                                                                                                                                            >  2:168149/1‑151 (MQ=255)      gcgTTTGCTGCTTTTACCATGGGAACGCTGACTGATTTCTGGCGGCAGAGTGATGAAGCAGAGTTTACTGGGGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACAGGGTGGTAGTCATCTGTCCGGGGCg                                                                                                                                           >  2:244866/1‑149 (MQ=255)          ttGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGTCCGGGGCGTATTg                                                                                                                                      >  2:637970/1‑150 (MQ=255)           tgctgcTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGTCCGGGGCGTATTg                                                                                                                                      >  2:593389/1‑149 (MQ=255)             ctgctTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGTCCGGGGCGTATTgag                                                                                                                                    >  2:228350/1‑149 (MQ=255)                ctTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGTCCGGGGCGTATTGAGAGCt                                                                                                                                >  1:569275/1‑150 (MQ=255)                 ttttACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGTCCGGGGCGTATTGAGAGCTAc                                                                                                                              >  2:689945/1‑151 (MQ=255)                   ttACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGTCCGGGGCGTATTGAGAGCTACGt                                                                                                                            >  1:798783/1‑151 (MQ=255)                    tACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGTCCGGGGCGTATTGAGAGCTACGt                                                                                                                            <  1:895786/150‑1 (MQ=255)                           ggACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGTCCGGGGCGTATTGAGAGCTACGTAAAATATg                                                                                                                    <  2:666763/151‑1 (MQ=255)                            gACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGTCCGGGGCGTATTGAGAGCTACGTAAAATATGc                                                                                                                   >  2:892104/1‑151 (MQ=255)                                gCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGTCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGaa                                                                                                               <  2:891631/151‑1 (MQ=255)                                      tgaTTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGTCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGcc                                                                                                         >  1:220018/1‑151 (MQ=255)                                        aTTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGTCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTa                                                                                                       <  2:474856/151‑1 (MQ=255)                                          ttCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGTCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTa                                                                                                       <  2:538963/149‑1 (MQ=255)                                          ttCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGTCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATg                                                                                                     >  2:766377/1‑151 (MQ=255)                                           tCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGTCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATg                                                                                                     >  2:315682/1‑150 (MQ=255)                                                 gTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGTCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGtt                                                                                              >  2:710381/1‑151 (MQ=255)                                                   cAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGTCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTcc                                                                                            >  1:127331/1‑151 (MQ=255)                                                    aGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGTCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTcc                                                                                            >  2:676560/1‑150 (MQ=255)                                                    aGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGTCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTcc                                                                                            >  2:653511/1‑150 (MQ=255)                                                         gatgaAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGTCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTg                                                                                       <  1:10881/150‑1 (MQ=255)                                                             aaGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGTCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGtt                                                                                  <  1:69060/151‑1 (MQ=255)                                                               gCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGTCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTg                                                                                <  2:134500/151‑1 (MQ=255)                                                                cAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGTCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGa                                                                               <  2:332081/151‑1 (MQ=255)                                                                              gtgGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGTCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAatcatc                                                                  <  1:266510/150‑1 (MQ=255)                                                                              gtgGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGTCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCg                                                                 <  2:335942/151‑1 (MQ=255)                                                                                     aCATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGTCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATcg                                                          <  2:829734/151‑1 (MQ=255)                                                                                          ccGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGTCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGc                                                     <  2:852745/151‑1 (MQ=255)                                                                                           cGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGTCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGc                                                     >  2:80859/1‑150 (MQ=255)                                                                                            ggTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGTCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGc                                                     >  2:624594/1‑149 (MQ=255)                                                                                              tGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGTCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAggg                                                 >  2:599735/1‑151 (MQ=255)                                                                                                 gcTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGTCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACg                                              >  2:714653/1‑151 (MQ=255)                                                                                                    ttGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGTCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGt                                             <  2:339030/149‑1 (MQ=255)                                                                                                          gTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGTCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCg                                     >  1:75100/1‑151 (MQ=255)                                                                                                          gTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGTCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCg                                     <  2:173249/151‑1 (MQ=255)                                                                                                                 cgcACAGCACCATGACCGGGTGGTAGTCATCTGTCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGtt                               >  2:146211/1‑150 (MQ=255)                                                                                                                       gCACCATGACCGGGTGGTAGTCATCTGTCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGAt                        <  1:168173/151‑1 (MQ=255)                                                                                                                         aCCATGACCGGGTGGTAGTCATCTGTCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATcc                      <  2:123405/151‑1 (MQ=255)                                                                                                                           cATGACCGGGTGGTAGTCATCTGTCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGc                    >  1:790717/1‑151 (MQ=255)                                                                                                                                ccGGGTGGTAGTCATCTGTCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCAtctc               <  1:426389/151‑1 (MQ=255)                                                                                                                                    gtggtAGTCATCTGTCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGc           >  2:555621/1‑151 (MQ=255)                                                                                                                                    gtggtAGTCATCTGTCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGc           >  1:858786/1‑151 (MQ=255)                                                                                                                                      ggtAGTCATCTGTCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCa          <  1:188482/150‑1 (MQ=255)                                                                                                                                         aGTCATCTGTCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGtt      >  2:638768/1‑151 (MQ=255)                                                                                                                                         aGTCATCTGTCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGtt      <  2:219992/151‑1 (MQ=255)                                                                                                                                          gTCATCTGTCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTAAGACATGTACCATTTGGGGTTTAATGTCTTAATAATCGACCATCGCGGGCTGGGACAGTCCGGTCGCATGTTAGCCGATCCGCATCTCGGGCATGtt      >  2:872193/1‑150 (MQ=255)                                                                                                                                             aTCTGTCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATc  >  1:241114/1‑151 (MQ=255)                                                                                                                                             aTCTGTCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATc  >  2:377904/1‑151 (MQ=255)                                                                                                                                                  |                                                                                                                                                  AAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGCCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATC  >  NC_000913/4009204‑4009494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑