Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,234,063	T→C	R102R (CGT→CGC)	pgi →	glucosephosphate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,234,063	0	T	C	100.0%	184.3 / NA	52	R102R (CGT→CGC)	pgi	glucosephosphate isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/26);  total (26/26)

TCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGTAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTTCTCAGAAGCGATTATTTCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTGACG  >  NC_000913/4233917‑4234205                                                                                                                                                   |                                                                                                                                               tCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGCAAcc                                                                                                                                            >  2:125445/1‑151 (MQ=255)      aaaCCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGCAACCGTAGc                                                                                                                                       <  1:854931/151‑1 (MQ=255)          cGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGCAACCGTAGCAAt                                                                                                                                    >  1:698930/1‑150 (MQ=255)          cGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGCAACCGTAGCAAt                                                                                                                                    >  2:433840/1‑150 (MQ=255)                 cTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGCAACCGTAGCAATACCCCGAt                                                                                                                            >  2:707801/1‑151 (MQ=255)                   gAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGAGTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGGGCAACCGTAGCAATACCCCGAttt                                                                                                                          >  1:126890/1‑151 (MQ=255)                        gaCGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGCAACCGTAGCAATACCCCGATTTTGGtt                                                                                                                     >  2:269638/1‑151 (MQ=255)                         aCGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGCAACCGTAGCAATACCCCGATTTTGGTTg                                                                                                                    >  2:691345/1‑151 (MQ=255)                             tGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGCAACCGTAGCAATACCCCGATTTTGGTTGATgg                                                                                                                >  2:710582/1‑151 (MQ=255)                              ggCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGCAACCGTAGCAATACCCCGATTTTGGTTGATgg                                                                                                                <  2:552989/150‑1 (MQ=255)                                cGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGCAACCGTAGCAATACCCCGATTTTGGTTGATGGCa                                                                                                              >  2:214273/1‑150 (MQ=255)                                 gAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGCAACCGTAGCAATACCCCGATTTTGGTTGATGGCaa                                                                                                             >  2:473779/1‑150 (MQ=255)                                  aaaTTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGCAACCGTAGCAATACCCCGATTTTGGTTGATGGCaa                                                                                                             >  1:233816/1‑149 (MQ=255)                                   aaTTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGCAACCGTAGCAATACCCCGATTTTGGTTGATGGCaa                                                                                                             >  1:16774/1‑148 (MQ=255)                                       aCAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGCAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTa                                                                                                      <  2:710460/151‑1 (MQ=255)                                        cAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGCAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTa                                                                                                      >  2:103138/1‑150 (MQ=255)                                             tCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGCAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCg                                                                                                >  1:397694/1‑151 (MQ=255)                                                  cGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGCAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGt                                                                                           >  1:768766/1‑151 (MQ=255)                                                    aaaGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGCAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCa                                                                                         <  1:61362/151‑1 (MQ=255)                                                       gagTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGCAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAAcg                                                                                      <  1:745925/151‑1 (MQ=255)                                                                cTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGCAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTgg                                                                             >  1:461991/1‑151 (MQ=255)                                                                  ggcgggcgCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGCAACCGTAGCAATCCCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTg                                                                              <  1:447175/148‑1 (MQ=255)                                                                  ggcgggcgCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGCAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGc                                                                                <  1:128891/146‑1 (MQ=255)                                                                   gcgggcgCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGCAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTGgaga                                                                          <  2:875597/151‑1 (MQ=255)                                                                     gggcgCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGCAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTGgaga                                                                          >  2:676338/1‑149 (MQ=255)                                                                       gcgcGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGCAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTGGAGAAGAt                                                                      <  1:439721/151‑1 (MQ=255)                                                                              tAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGCAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAAc                                                                <  2:103297/150‑1 (MQ=255)                                                                                   cGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGCAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTtctc                                                          <  2:458837/151‑1 (MQ=255)                                                                                   cGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGCAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTtctc                                                          >  2:394955/1‑151 (MQ=255)                                                                                      tGTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGCAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTtctc                                                          >  2:855415/1‑148 (MQ=255)                                                                                       gTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGCAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTTCTCAGaa                                                      <  1:483260/151‑1 (MQ=255)                                                                                         tctcTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGCAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTTCTCAGAAg                                                     <  1:466382/150‑1 (MQ=255)                                                                                         tctcTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGCAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTTCTCAGAAg                                                     <  1:390505/150‑1 (MQ=255)                                                                                           tctGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGCAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTTCTCAGAAGCg                                                   >  1:674640/1‑150 (MQ=255)                                                                                              ggCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGCAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTTCTCAGAAGCGat                                                 <  1:622753/149‑1 (MQ=255)                                                                                                cGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGCAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTTCTCAGAAGCGattatt                                             >  1:439898/1‑151 (MQ=255)                                                                                                cGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGCAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTTCTCAGAAGCGattatt                                             <  2:804238/151‑1 (MQ=255)                                                                                                   gaagaTCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGCAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTTCTCAGAAGCGATTATTTcc                                          <  1:254079/151‑1 (MQ=255)                                                                                                     agaTCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGCAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTTCTCAGAAGCGATTATTTCCgg                                        <  1:161815/151‑1 (MQ=255)                                                                                                      gaTCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGCAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTTCTCAGAAGCGATTATTTCCGGt                                       >  1:213354/1‑151 (MQ=255)                                                                                                      gaTCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGCAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTTCTCAGAAGCGATTATTTCCGGt                                       <  1:781915/151‑1 (MQ=255)                                                                                                         cAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGCAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTTCTCAGAAGCGATTATTTCCGGTg                                      >  1:451909/1‑149 (MQ=255)                                                                                                          aaCCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGCAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTTCTCAGAAGCGATTATTTCCGGTGAGt                                   <  1:269664/151‑1 (MQ=255)                                                                                                             cGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGCAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTTCTCAGAAGCGATTATTTCCGGTGAGTgg                                 >  2:840287/1‑150 (MQ=255)                                                                                                              gCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGCAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTTCTCAGAAGCGATTATTTCCGGTGAGTGGaa                               <  1:668115/151‑1 (MQ=255)                                                                                                              gCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGCAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTTCTCAGAAGCGATTATTTCCGGTGAGTGGa                                >  2:632232/1‑150 (MQ=255)                                                                                                                   gAAAACCGCGCCGTGCTGCACGTAGCGCTGCGCAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTTCTCAGAAGCGATTATTTCCGGTGAGTGGAAAGGt                           <  1:688477/150‑1 (MQ=255)                                                                                                                    aaaaCCGCGCCGTGCTGCACGTAGCGCTGCGCAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTTCTCAGAAGCGATTATTTCCGGTGAGTGGAAAGGtt                          >  1:384423/1‑150 (MQ=255)                                                                                                                          gcgcCGTGCTGCACGTAGCGCTGCGCAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTTCTCAGAAGCGATTATTTCCGGTGAGTGGAAAGGTTATACCgg                   <  1:319019/151‑1 (MQ=255)                                                                                                                                        gTAGCGCTGCGCAACCGTAGCAATACCCCGATTTTGGTTGATGGTAAAGACGTAATTCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTTCTCAGAAGCGATTATTTCCGGTGAGTGGAAAGGTTATACCGGCAAAGCTATCACTg     <  2:527826/151‑1 (MQ=255)                                                                                                                                          aGCGCTGCGCAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTTCTCAGAAGCGATTATTTCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTGa    <  2:584006/150‑1 (MQ=255)                                                                                                                                           gcgcTGCGCAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTTCTCAGAAGCGATTATTTCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTGACg  <  1:433887/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                               TCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGTAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTTCTCAGAAGCGATTATTTCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTGACG  >  NC_000913/4233917‑4234205

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑