Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,265,952	A→G	V57V (GTA→GTG)	alr →	alanine racemase, biosynthetic, PLP‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,265,952	0	A	G	100.0%	198.1 / NA	60	V57V (GTA→GTG)	alr	alanine racemase, biosynthetic, PLP‑binding
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/32);  total (28/32)

ACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTGCGCAACATTTTCATACCGCCGTGCATAACGAAGAACAGCTGGCT  >  NC_000913/4265807‑4266099                                                                                                                                                  |                                                                                                                                                    aCCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTGGCCCg                                                                                                                                                >  2:698572/1‑151 (MQ=255)  ccgccgCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTGGCCCg                                                                                                                                                <  1:63311/150‑1 (MQ=255)     ccgcGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTGGCCCGtctc                                                                                                                                            <  2:480758/151‑1 (MQ=255)       gcgcTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTGGCCCGTCTCg                                                                                                                                           >  1:878420/1‑150 (MQ=255)         gcTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTGGCCCGTCTCga                                                                                                                                          >  2:636617/1‑149 (MQ=255)             tGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTGGCCCGTCTCGaagaag                                                                                                                                     >  2:895089/1‑150 (MQ=255)               cGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTGGCCCGTCTCGAAGAAGctc                                                                                                                                  >  2:93670/1‑151 (MQ=255)               cGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGGTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTGGCCCGTCTCGAAGAAGctc                                                                                                                                  >  1:658642/1‑151 (MQ=255)                   acaACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTGGCCCGTCTCGAAGAAGCTCTGCg                                                                                                                              >  2:764659/1‑151 (MQ=255)                     aaCCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTGGCCCGTCTCGAAGAAGCTCTGCg                                                                                                                              <  2:744690/149‑1 (MQ=255)                       ccTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTGGCCCGTCTCGAAGAAGCTCTGCGACt                                                                                                                           <  2:524412/150‑1 (MQ=255)                                   tCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTGGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCgggg                                                                                                                 <  2:531254/148‑1 (MQ=255)                                       gAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTGGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATc                                                                                                           >  1:139194/1‑150 (MQ=255)                                       gAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTGGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATc                                                                                                           >  2:16811/1‑150 (MQ=255)                                            ggCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTGGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCaaa                                                                                                     <  1:16833/151‑1 (MQ=255)                                            ggCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTGGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCaa                                                                                                      >  1:425546/1‑150 (MQ=255)                                                   gCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTGGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTa                                                                                               <  2:737506/150‑1 (MQ=255)                                                      aGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTGGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGt                                                                                           <  1:282019/151‑1 (MQ=255)                                                      aGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTGGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGt                                                                                           <  2:35483/151‑1 (MQ=255)                                                        tAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTGGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGt                                                                                           >  2:451825/1‑149 (MQ=255)                                                          aaaTGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTGGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACt                                                                                       >  1:810251/1‑151 (MQ=255)                                                            aTGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTGGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCg                                                                                     <  2:425501/151‑1 (MQ=255)                                                              ggTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTGGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGaa                                                                                   <  1:188140/151‑1 (MQ=255)                                                                 tGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTGGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTGGAAgg                                                                                 <  2:260213/150‑1 (MQ=255)                                                                     gtggtgAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTGGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCtttt                                                                            >  1:689643/1‑151 (MQ=255)                                                                       ggtgAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTGGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCttttt                                                                           <  1:82528/150‑1 (MQ=255)                                                                        gtgAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTGGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTg                                                                         >  2:1915/1‑151 (MQ=255)                                                                          gAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTGGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGa                                                                        <  1:43566/150‑1 (MQ=255)                                                                           aaaGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTGGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATg                                                                      <  1:352409/151‑1 (MQ=255)                                                                            aaGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTGGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTAGATg                                                                      >  2:736012/1‑150 (MQ=255)                                                                                gAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTGGCCCGTCTCGAAGAAGCTCTGCGACTGCGCGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATg                                                                      >  1:667678/1‑146 (MQ=255)                                                                                 aaCGCTGATGGTCACGGTCTTCTTGAGACCCCGTGAACGCTCCCCGATGCTGTCGCCTTTGGCGCGGCCCGTCTCGAAGTAGCTCTGCTACTGCGTGCGGGGTGATCTACCAAACCTGTACTCTTTTTCGCAGGCGTTTTTGATggc                                                                    >  2:702843/1‑145 (MQ=255)                                                                                  aCGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTGGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCag                                                                  >  1:272074/1‑148 (MQ=255)                                                                                       tATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTGGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTg                                                           <  1:828774/150‑1 (MQ=255)                                                                                         tGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTGGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCcg                                                        >  2:66472/1‑151 (MQ=255)                                                                                          ggTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTGGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCcga                                                       <  1:279663/151‑1 (MQ=255)                                                                                              aCGGACTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGACTTTGGCGTGGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGAGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGACAGAGATCTGCCGACGAt                                                   >  2:613837/1‑151 (MQ=255)                                                                                               cGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTGGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGAtt                                                  <  1:788692/151‑1 (MQ=255)                                                                                                ggTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTGGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGAttt                                                 >  2:344287/1‑151 (MQ=255)                                                                                                ggTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTGGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGAttt                                                 >  2:595441/1‑151 (MQ=255)                                                                                                   cttcttGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTGGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTg                                              >  2:880843/1‑151 (MQ=255)                                                                                                     tcttGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTGGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCt                                               <  1:66494/148‑1 (MQ=255)                                                                                                       ttGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTGGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTgcg                                            >  1:311003/1‑149 (MQ=255)                                                                                                             ccGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTGGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTGCGCAACAttt                                    <  2:779339/151‑1 (MQ=255)                                                                                                                cgcgAACGCTCCCCGATGCTGACGCCTTTGGCGTGGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTGCGCAACATTTTc                                  <  1:501695/150‑1 (MQ=255)                                                                                                                  cgAACGCTCCCCGATGCTGACGCCTTTGGCGTGGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTGCGCAACATTTTCATa                               <  2:591868/151‑1 (MQ=255)                                                                                                                   gAACGCTCCCCGATGCTGACGCCTTTGGCGTGGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTGCGCAACATTTTCATAc                              <  1:880914/151‑1 (MQ=255)                                                                                                                          ccccGATGCTGACGCCTTTGGCGTGGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAACCCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTGCGCAACATTTTCATACCGCCGt                        <  2:574866/150‑1 (MQ=255)                                                                                                                           cccGATGCTGACGCCTTTGGCGTGGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTGCGCAACATTTTCATACCGCCGTGc                      >  1:901521/1‑151 (MQ=255)                                                                                                                             cGATGCTGACGCCTTTGGCGTGGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTGCGCAACATTTTCATACCGCCGTGCAt                    >  2:169570/1‑151 (MQ=255)                                                                                                                              gATGCTGACGCCTTTGGCGTGGCTCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAACCCTGTACTGTTACGCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTGCGCAACATTTTCATACCGCCGTGCAt                    <  1:569440/150‑1 (MQ=255)                                                                                                                               aTGCTGACGCCTTTGGCGTGGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTGCGCAACATTTTCATACCGCCGTGCATaa                  <  2:514394/151‑1 (MQ=255)                                                                                                                                 gCTGACGCCTTTGGCGTGGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTGCGCAACATTTTCATACCGCCGTGCATAAc                 <  1:600983/150‑1 (MQ=255)                                                                                                                                  cTGACGCCTTTGGCGTGGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTGCGCAACATTTTCATACCGCCGTGCATAACg                >  2:354884/1‑150 (MQ=255)                                                                                                                                   tGACGCCTTTGGCGTGGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTGCGCAACATTTTCATACCGCCGTGCATAACga               >  2:425477/1‑150 (MQ=255)                                                                                                                                    gACGCCTTTGGCGTGGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTGCGCAACATTTTCATACCGCCGTGCATAACgaa              <  1:177434/150‑1 (MQ=255)                                                                                                                                      cGCCTTTGGCGTGGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTGCGCAACATTTTCATACCGCCGTGCATAACgaagaa           <  2:741008/151‑1 (MQ=255)                                                                                                                                            tGGCGTGGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTGCGCAACATTTTCATACCGCCGTGCATAACGAAGAACAgct      <  2:387207/150‑1 (MQ=255)                                                                                                                                               cGTGGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTGCGCAACATTTTCATACCGCCGTGCATAACGAAGAACAgctggct  <  2:280171/151‑1 (MQ=255)                                                                                                                                               cGTGGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTGCGCAACATTTTCATACCGCCGTGCATAACGAAGAACAgctggct  <  2:807511/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                    ACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTGCGCAACATTTTCATACCGCCGTGCATAACGAAGAACAGCTGGCT  >  NC_000913/4265807‑4266099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑