Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,286,249 T→C T375A (ACC→GCC)  acs ← acetyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,286,2490TC97.7% 146.0 / ‑5.4 44T375A (ACC→GCC) acsacetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (19/24);  total (20/24)
Fisher's exact test for biased strand distribution p-value = 4.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

TGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTGGGTACGCCTTCAAACATCAGCGTGGT  >  NC_000913/4286108‑4286396
                                                                                                                                             |                                                                                                                                                   
tGAATCCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGa                                                                                                                                             <  2:660901/150‑1 (MQ=255)
  aaaCCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATc                                                                                                                                           <  2:349773/150‑1 (MQ=255)
     ccgccgGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCtt                                                                                                                                       <  2:783943/151‑1 (MQ=255)
      cgccgGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCttt                                                                                                                                      >  2:579836/1‑151 (MQ=255)
             ttCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGCTTGTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCTACGGCACCGAGAATGCCCAGCGACGAACGGTCGGTGCCTTCGATCGCTTTATCGCCt                                                                                                                               >  2:642954/1‑151 (MQ=255)
               cGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTc                                                                                                                             >  2:124132/1‑151 (MQ=255)
                ggTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTcc                                                                                                                            >  1:776342/1‑151 (MQ=255)
                   cTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGcc                                                                                                                         >  2:358053/1‑151 (MQ=255)
                    tGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGcc                                                                                                                         >  2:867008/1‑150 (MQ=255)
                     gCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCa                                                                                                                        <  2:748601/150‑1 (MQ=255)
                        accaGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAg                                                                                                                    <  1:678710/151‑1 (MQ=255)
                         ccaGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAg                                                                                                                    <  2:596849/150‑1 (MQ=255)
                          caGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAgcg                                                                                                                  >  1:312669/1‑151 (MQ=255)
                                 cGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGa                                                                                                            <  2:247983/150‑1 (MQ=255)
                                       ccGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGt                                                                                                     <  1:233371/151‑1 (MQ=255)
                                              tttCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCg                                                                                              >  2:864266/1‑151 (MQ=255)
                                               ttCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCg                                                                                              <  2:497773/150‑1 (MQ=255)
                                                   cGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGtat                                                                                          <  1:105888/150‑1 (MQ=255)
                                                   cGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGt                                                                                            <  2:516714/148‑1 (MQ=255)
                                                      tGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATaga                                                                                       <  1:80176/150‑1 (MQ=255)
                                                         cGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAat                                                                                   >  2:890006/1‑151 (MQ=255)
                                                          gATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAat                                                                                   >  1:209068/1‑150 (MQ=255)
                                                                   ccAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTg                                                                          <  1:56446/150‑1 (MQ=255)
                                                                   ccAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTg                                                                          >  2:366053/1‑150 (MQ=255)
                                                                    cAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGAt                                                                        >  2:647498/1‑151 (MQ=255)
                                                                      gTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGc                                                                      <  1:102577/151‑1 (MQ=255)
                                                                      gTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGc                                                                      >  1:318296/1‑151 (MQ=255)
                                                                       tACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCt                                                                     >  2:295314/1‑151 (MQ=255)
                                                                           aCTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTc                                                                 >  1:182032/1‑151 (MQ=255)
                                                                              cccACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTcca                                                               <  1:630745/150‑1 (MQ=255)
                                                                                cACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTccacca                                                            <  2:677843/151‑1 (MQ=255)
                                                                                   gCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCaccacc                                                          >  2:322280/1‑150 (MQ=255)
                                                                                   gCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCt                                                         <  2:383241/151‑1 (MQ=255)
                                                                                   gCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCt                                                         <  2:524080/151‑1 (MQ=255)
                                                                                      tCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTg                                                        <  2:306282/149‑1 (MQ=255)
                                                                                            ttaagaggCTCGCCCACGGGACCGAGAATGCGCATCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGTGAATATTGTCCTGATGCTTGTCCACCGCCTGCGCCATa                                                 <  2:630451/144‑1 (MQ=255)
                                                                                                  ggCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGc                                            >  2:105858/1‑149 (MQ=255)
                                                                                                  gccTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCa                                           <  1:729623/148‑1 (MQ=255)
                                                                                                   gCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAgg                                         >  2:180061/1‑151 (MQ=255)
                                                                                                      cGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGc                                        <  1:363663/149‑1 (MQ=255)
                                                                                                                   gagaATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTGGGt                         >  2:853607/1‑151 (MQ=255)
                                                                                                                              gCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTGGGTACGCCTTCaa               <  2:457524/150‑1 (MQ=255)
                                                                                                                               cgacgaACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTGGGTACGCCTTCAAAc             <  2:288798/151‑1 (MQ=255)
                                                                                                                                   gaACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTGGGTACGCCTTCAAACATc          >  2:785226/1‑150 (MQ=255)
                                                                                                                                        gtcggcGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTGGGTACGCCTTCAAACATCAGCGTg    <  1:262303/151‑1 (MQ=255)
                                                                                                                                        gtcggcGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTGGGTACGCCTTCAAACATCAGCGTg    >  2:220526/1‑151 (MQ=255)
                                                                                                                                          cggcGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTGGGTACGCCTTCAAACATCAGCGTGGt  >  1:868162/1‑151 (MQ=255)
                                                                                                                                             |                                                                                                                                                   
TGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTGGGTACGCCTTCAAACATCAGCGTGGT  >  NC_000913/4286108‑4286396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑