Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,358,354	A→G	T88T (ACT→ACC)	cadA ←	lysine decarboxylase, acid‑inducible

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,358,354	0	A	G	100.0%	237.5 / NA	64	T88T (ACT→ACC)	cadA	lysine decarboxylase, acid‑inducible
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/36);  total (28/36)

TAAACAGTGCTTTAGTCAGCGGAGGCAGAATAGTGTTGATATATTCGTCAGTGGTCTGCTTGATCTTATTAGCAATATCTTCAGCAGCACCCAGCGCATATTCAAAGAAGCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGAGTGGAATACGTATTAGCGAACGCGTACAACGGCAGGTTCTCGTTCATTTTGCTAATTTCTTCGCACAGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGATCAGTTTTAATAAGTCGTCA  >  NC_000913/4358206‑4358501                                                                                                                                                     |                                                                                                                                                    tAAACAGTGCTTTAGTCAGCGGAGGCAGAATAGTGTTGATATATTCGTCAGTGGTCTGCTTGATCTTATTAGCAATATCTTCAGCAGCACCCAGCGCATATTCAAAGAAGCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGt                                                                                                                                                   >  1:254627/1‑151 (MQ=255) tAAACAGTGCTTTAGTCAGCGGAGGCAGAATAGTGTTGATATATTCGTCAGTGGTCTGCTTGATCTTATTAGCAATATCTTCAGCAGCACCCAGCGCATATTCAAAGAAGCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGt                                                                                                                                                   >  2:642748/1‑151 (MQ=255)  aaaCAGTGCTTTAGTCAGCGGAGGCAGAATAGTGTTGATATATTCGTCAGTGGTCTGCTTGATCTTATTAGCAATATCTTCAGCAGCACCCAGCGCATATTCAAAGAAGCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGTg                                                                                                                                                  >  2:640555/1‑151 (MQ=255)   aaCAGTGCTTTAGTCAGCGGAGGCAGAATAGTGTTGATATATTCGTCAGTGGTCTGCTTGATCTTATTAGCAATATCTTCAGCAGCACCCAGCGCATATTCAAAGAAGCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGTgg                                                                                                                                                 <  2:491851/151‑1 (MQ=255)         gCTTTAGTCAGCGGAGGCAGAATAGTGTTGATATATTCGTCAGTGGTCTGCTTGATCTTATTAGCAATATCTTCAGCAGCACCCAGCGCATATTCAAAGAAGCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGTGGAATACg                                                                                                                                           <  1:292182/151‑1 (MQ=255)              aGTCAGCGGAGGCAGAATAGTGTTGATATATTCGTCAGTGGTCTGCTTGATCTTATTAGCAATATCTTCAGCAGCACCCAGCGCATATTCAAAGAAGCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGTGGAATACGTAtt                                                                                                                                       >  2:453135/1‑150 (MQ=255)              aGTCAGCGGAGGCAGAATAGTGTTGATATATTCGTCAGTGGTCTGCTTGATCTTATTAGCAATATCTTCAGCAGCACCCAGCGCATATTCAAAGAAGCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGTGGAATACGTATTa                                                                                                                                      <  2:831393/151‑1 (MQ=255)                 cAGCGGAGGCAGAATAGTGTTGATATATTCGTCAGTGGTCTGCTTGATCTTATTAGCAATATCTTCAGCAGCACCCAGCGCATATTCAAAGAAGCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGTGGAATACGTATTAGCg                                                                                                                                   <  1:397163/151‑1 (MQ=255)                  aGCGGAGGCAGAATAGTGTTGATATATTCGTCAGTGGTCTGCTTGATCTTATTAGCAATATCTTCAGCAGCACCCAGCGCATATTCAAAGAAGCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGTGGAATACGTATTAGCg                                                                                                                                   >  2:716975/1‑150 (MQ=255)                  aGCGGAGGCAGAATAGTGTTGATATATTCGTCAGTGGTCTGCTTGATCTTATTAGCAATATCTTCAGCAGCACCCAGCGCATATTCAAAGAAGCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGTGGAATACGTATTAGCg                                                                                                                                   <  1:356575/150‑1 (MQ=255)                    cGGAGGCAGAATAGTGTTGATATATTCGTCAGTGGTCTGCTTGATCTTATTAGCAATATCTTCAGCAGCACCCAGCGCATATTCAAAGAAGCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGTGGAATACGTATTAGCGAAc                                                                                                                                <  2:754892/151‑1 (MQ=255)                     ggAGGCAGAATAGTGTTGATATATTCGTCAGTGGTCTGCTTGATCTTATTAGCAATATCTTCAGCAGCACCCAGCGCATATTCAAAGAAGCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGTGGAATACGTATTAGCGAAcg                                                                                                                               <  2:320141/151‑1 (MQ=255)                          cAGAATAGTGTTGATATATTCGTCAGTGGTCTGCTTGATCTTATTAGCAATATCTTCAGCAGCACCCAGCGCATATTCAAAGAAGCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGTGGAATACGTATTAGCGAACGCGTAc                                                                                                                          <  2:834792/151‑1 (MQ=255)                             aaTAGTGTTGATATATTCGTCAGTGGTCTGCTTGATCTTATTAGCAATATCTTCAGCAGCACCCAGCGCATATTCAAAGAAGCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGTGGAATACGTATTAGCGAACGCGTACAAc                                                                                                                       <  1:670428/151‑1 (MQ=255)                                   gtTGATATATTCGTCAGTGGTCTGCTTGATCTTATTAGCAATATCTTCAGCAGCACCCAGCGCATATTCAAAGAAGCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGTGGAATACGTATTAGCGAACGCGTACAACGGCAg                                                                                                                  <  1:301260/150‑1 (MQ=255)                                       atatatTCGTCAGTGGTCTGCTTGATCTTATTAGCAATATCTTCAGCAGCACCCAGCGCATATTCAAAGAAGCTAATCTGTAAACGCAGGGCATTCAGGCTTACATCGAGGGTGGAATACGTATTAGCGAACGCGTACAACGGCAGGTtc                                                                                                              >  2:546926/1‑150 (MQ=255)                                        tatatTCGTCAGTGGTCTGTTTGATCGTATTAGCAATATCTTCAGCAGCACCCAGCGCATATTCAAAGAAGCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGATGGTAGAATACGTATTAGCGAACGCGTACAACGGCAgga                                                                                                                <  2:780370/147‑2 (MQ=255)                                         atatTCGTCAGTGGTCTGCTTGATCTTATTAGCAATATCTTCAGCAGCACCCAGCGCATATTCAAAGAAGCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGTGGAATACGTATTAGCGAACGCGTACAACGGCAGGTtctc                                                                                                            <  2:763187/150‑1 (MQ=255)                                         atatTCGTCAGTGGTCTGCTTGATCTTATTAGCAATATCTTCAGCAGCACCCAGCGCATATTCAAAGAAGCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGTGGAATACGTATTAGCGAACGCGTACAACGGCAGGTtctc                                                                                                            <  2:826503/150‑1 (MQ=255)                                         atatTCGTCAGTGGTCTGCTTGATCTTATTAGCAATATCTTCAGCAGCACCCAGCGCATATTCAAAGAAGCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGTGGAATACGTATTAGCGAACGCGTACAACGGCAGGTtctc                                                                                                            <  2:197938/150‑1 (MQ=255)                                           atTCGTCAGTGGTCTGCTTGATCTTATTAGCAATATCTTCAGCAGCACCCAGCGCATATTCAAAGAAGCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGTGGAATACGTATTAGCGAACGCGTACAACGGCAGGTTCTCGt                                                                                                          <  2:557738/150‑1 (MQ=255)                                                     ggTCTGCTTGATCTTATTAGCAATATCTTCAGCAGCACCCAGCGCATATTCAAAGAAGCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGTGGAATACGTATTAGCGAACGCGTACAACGGCAGGTTCTCGTTCATTTTGCt                                                                                                <  1:731474/150‑1 (MQ=255)                                                      gTCTGCTTGATCTTATTAGCAATATCTTCAGCAGCACCCAGCGCATATTCAAAGAAGCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGTGGAATACGTATTAGCGAACGCGTACAACGGCAGGTTCTCGTTCATTTTGCTa                                                                                               >  1:92799/1‑150 (MQ=255)                                                          gCTTGATCTTATTAGCAATATCTTCAGCAGCACCCAGCGCATATTCAAAGAAGCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGTGGAATACGTATTAGCGAACGCGTACAACGGCAGGTTCTCGTTCATTTTGCTAAt                                                                                             <  2:581529/148‑1 (MQ=255)                                                           cTTGATCTTATTAGCAATATCTTCAGCAGCACCCAGCGCATATTCAAAGAAGCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGTGGAATACGTATTAGCGAACGCGTACAACGGCAGGTTCTCGTTCATTTTGCTAATttct                                                                                         >  2:476984/1‑151 (MQ=255)                                                             tGATCTTATTAGCAATATCTTCAGCAGCACCCAGCGCATATTCAAAGAAGCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGTGGAATACGTATTAGCGAACGCGTACAACGGCAGGTTCTCGTTCATTTTGCTAATttcttc                                                                                       >  1:822657/1‑151 (MQ=255)                                                              gATCTTATTAGCAATATCTTCAGCAGCACCCAGCGCATATTCAAAGAAGCTAATTTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGTGGAATACGTATTAGCGAACGCGTACAACGGCAGGTTCTCGTTCATTTTGCTAATTTCTTCg                                                                                      >  1:205211/1‑151 (MQ=255)                                                                tCTTATTAGCAATATCTTCAGCAGCACCCAGCGCATATTCAAAGAAGCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGTGGAATACGTATTAGCGAACGCGTACAACGGCAGGTTCTCGTTCATTTTGCTAATTTCTTCGc                                                                                     >  1:184178/1‑150 (MQ=255)                                                                tCTTATAAGCAATATCTTCAGCAGCACCCAGCGCATATTCAAAGAAGCTAATCTGTAAACCCAGGTCATTCAGGCTTACATCGAGGGGGGAAGACGTATTAGAGAACGCGTACAACGGCAGGTTCTCGTTCATTTTGCTAATTTTTTCGc                                                                                     >  2:745527/1‑150 (MQ=255)                                                                   tattaGCAATATCTTCAGCAGCACCCAGCGCATATTCAAAGAAGCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGTGGAATACGTATTAGCGAACGCGTACAACGGCAGGTTCTCGTTCATTTTGCTAATTTCTTCGcac                                                                                   >  1:880796/1‑149 (MQ=255)                                                                   tattaGCAATATCTTCAGCAGCACCCAGCGCATATTCAAAGAAGCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGTGGAATACGTATTAGCGAACGCGTACAACGGCAGGTTCTCGTTCATTTTGCTAATTTCTTCGca                                                                                    <  2:803393/148‑1 (MQ=255)                                                                    attaGCAATATCTTCAGCAGCACCCAGCGCATATTCAAAGAAGCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGTGGAATACGTATTAGCGAACGCGTACAACGGCAGGTTCTCGTTCATTTTGCTAATTTCTTCGCACAGc                                                                                >  1:276118/1‑151 (MQ=255)                                                                    attaGCAATATCTTCAGCAGCACCCAGCGCATATTCAAAGAAGCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGTGGAATACGTATTAGCGAACGCGTACAACGGCAGGTTCTCGTTCATTTTGCTAATTTCTTCGCACAGc                                                                                <  2:286136/151‑1 (MQ=255)                                                                     ttaGCAATATCTTCAGCAGCACCCAGCGCATATTCAAAGAAGCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGTGGAATACGTATTAGCGAACGCGTACAACGGCAGGTTCTCGTTCATTTTGCTAATTTCTTCGCACAGCt                                                                               >  1:550909/1‑151 (MQ=255)                                                                      taGCAATATCTTCAGCAGCACCCAGCGCATATTCAAAGAAGCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGTGGAATACGTATTAGCGAACGCGTACAACGGCAGGTTCTCGTTCATTTTGCTAATTTCTTCGCACAGCt                                                                               <  2:329384/150‑1 (MQ=255)                                                                      taGCAATATCTTCAGCAGCACCCAGCGCATATTCAAAGAAGCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGTGGAATACGTATTAGCGAAAGCGTACAACGGCAGGTTCTCGTTCATTTTGCTAATTTCTTCGCACAGCTc                                                                              >  1:461664/1‑151 (MQ=255)                                                                            tatCTTCAGCAGCACCCAGCGCATATTCAAAGAAGCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGTGGAATACGTATTAGCGAACGCGTACAACGGCAGGTTCTCGTTCATTTTGCTAATTTCTTCGCACAGCTCGAGAtt                                                                        >  1:333047/1‑151 (MQ=255)                                                                             atCTTCAGCAGCACCCAGCGCATATTCAAAGAAGCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGTGGAATACGTATTAGCGAACGCGTACAACGGCAGGTTCTCGTTCATTTTGCTAATTTCTTCGCACAGCTCGAGAtt                                                                        <  1:583686/150‑1 (MQ=255)                                                                                ttCAGCAGCACCCAGCGCATATTCAAAGAAGCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGTGGAATACGTATTAGCGAACGCGTACAACGGCAGGTTCTCGTTCATTTTGCTGATTTCTTCGCACAGCTCGAGATTatat                                                                    >  2:132777/1‑151 (MQ=255)                                                                                ttCAGCAGCACCCAGCGCATATTCAAAGAAGCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGTGGAATACGTATTAGCGAACGCGTACAACGGCAGGTTCTCGTTCATTTTGCTAATTTCTTCGCACAGCTCGAGATTatat                                                                    >  2:710385/1‑151 (MQ=255)                                                                                  cagcagCACCCAGCGCATATTCAAAGAAGCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGTGGAATACGTATTAGCGAACGCGTACAACGGCAGGTTCTCGTTCATTTTGCTAATTTCTTCGCACAGCTCGAGATTATAttt                                                                  >  2:424825/1‑151 (MQ=255)                                                                                   agcagcACCCAGCGCATATTCAAAGAAGCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGTGGAATACGTATTAGCGAACGCGTACAACGGCAGGTTCTCGTTCATTTTGCTAATTTCTTCGCACAGCTCGAGATTATATTTa                                                                 <  2:463572/151‑1 (MQ=255)                                                                                   agcagcACCCAGCGCATATTCAAAGAAGCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGTGGAATACGTATTAGCGAACGCGTACAACGGCAGGTTCTCGTTCATGTTGCTAATTTCTTCGCACAGCTCGAGATTATATTTa                                                                 <  1:687640/151‑1 (MQ=255)                                                                                       gcaCCCAGCGCATATTCAAAGAAGCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGTGGAATACGTATTAGCGAACGCGTACAACGGCAGGTTCTCGTTCATTTTGCTAATTTCTTCGCACAGCTCGAGATTATATTTATccc                                                             <  1:485232/151‑1 (MQ=255)                                                                                         aCCCAGCGCATATTCAAAGAAGCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGAGGAATACGTATTAGCGAACGCGTACAACGGCAGGTTCTCGTTCATTTTGCTAATTTCTTCGCACAGCTCGAGATTATATTTATccc                                                             >  1:746228/1‑149 (MQ=255)                                                                                            cAGCGCATATTCAAAGAAGCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGTGGAATACGTATTAGCGAACGCGTACAACGGCAGGTTCTCGTTCATTTTGCTAATTTCTTCGCACAGCTCGAGATTATATTTATCCCAGTCa                                                        <  1:191593/151‑1 (MQ=255)                                                                                              gcgcATATTCAAAGAAGCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGTGGAATACGTATTAGCGAACGCGTACAACGGCAGGTTCTCGTTCATTTTGCTAATTTCTTCGCACAGCTCGAGATTATATTTATCCCAGTCaaa                                                      <  1:706607/151‑1 (MQ=255)                                                                                               cgcATATTCAAAGAAGCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGTGGAATACGTATTAGCGAACGCGTACAACGGCAGGTTCTCGTTCATTTTGCTAATTTCTTCGCACAGCTCGAGATTATATTTATCCCAGTCaaa                                                      >  1:314121/1‑150 (MQ=255)                                                                                                 cATATTCAAAGAAGCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGTGGAATACGTATTAGCGAACGCGTACAACGGCAGGTTCTCGTTCATTTTGCTAATTTCTTCGCACAGCTCGAGATTATATTTATCCCAGTCAAAAAt                                                   <  2:407512/151‑1 (MQ=255)                                                                                                  atatTCAAAGAAGCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGTGGAATACGTATTAGCGAACGCGTACAACGGCAGGTTCTCGTTCATTTTGCTAATTTCTTCGCACAGCTCGAGATTATATTTATCCCAGTCAAAAAt                                                   >  1:520625/1‑150 (MQ=255)                                                                                                    atTCAAAGAAGCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGTGGAATACGTATTAGCGAACGCGTACAACGGCAGGTTCTCGTTCATTTTGCTAATTTCTTCGCACAGCTCGAGATTATATTTATCCCAGTCAAAAATaa                                                 <  1:608852/150‑1 (MQ=255)                                                                                                    atTCAAAGAAGCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGTGGAATACGTATTAGCGAACGCGTACAACGGCAGGTTCTCGTTCATTTTGCTAATTTCTTCGCACAGCTCGAGATTATATTTATCCCAGTCAAAAATAAc                                                <  2:10304/151‑1 (MQ=255)                                                                                                           gaagCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGTGGAATACGTATTAGCGAACGCGTACAACGGCAGGTTCTCGTTCATTTTGCTAATTTCTTCGCACAGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGcac                                         <  1:198694/151‑1 (MQ=255)                                                                                                                tAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGTGGAATACGTATTAGCGAACGCGTACAACGGCAGGTTCTCGTTCATTTTGCTAATTTCTTCGCACAGCTCGAGTGTATATTTATCCCAGTCAAAAATAACGCCGCACAGAcg                                    >  2:445768/1‑151 (MQ=255)                                                                                                                 aaTCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGTGGAATACGTATTAGCGAACGCGTACAACGGCAGGTTCTCGTTCATTTTGCTAATTTCTTCGCACAGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGAcgc                                   <  1:292404/151‑1 (MQ=255)                                                                                                                 aaTCTGTAAACGCAGGTCATTCAGGCTTACATCGAGGGTGGAATACGTATTAGCGAACGCGTACAACGGCAGGTTCTCGTTCATTTTGCTAATTTCTTCGCACAGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGAcgc                                   <  2:758625/151‑1 (MQ=255)                                                                                                                      gTAAACGCAGGTCATTCAGGCTTACATCGAGGGTGGAATACGTATTAGCGAACGCGTACAACGGCAGGTTCTCGTTCATTTTGCTAATTTCTTCGCACAGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCAt                               <  2:744305/150‑1 (MQ=255)                                                                                                                       tAAACGCAGGTCATTCAGGCTTACATCGAGGGTGGAATACGTATTAGCGAACGCGTACAACGGCAGGTTCTCGTTCATTTTGCTAATTTCTTCGCACAGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTg                             >  1:3187/1‑151 (MQ=255)                                                                                                                        aaaCGCAGGTCATTCAGGCTTACATCGAGGGTGGAATACGTATTAGCGAACGCGTACAACGGCAGGTTCTCGTTCATTTTGCTAATTTCTTCGCACAGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTg                             <  2:237804/150‑1 (MQ=255)                                                                                                                             cAGGTCATTCAGGCTTACATCGAGGGTGGAATACGTATTAGCGAACGCGTACAACGGCAGGTTCTCGTTCATTTTGCTAATTTCTTCGCACAGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCg                       <  2:505757/151‑1 (MQ=255)                                                                                                                                 tCATTCAGGCTTACATCGAGGGTGGAATACGTATTAGCGAACGCGTACAACGGCAGGTTCTCGTTCATTTTGCTAATTTCTTCGCACAGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGATc                    >  2:656439/1‑150 (MQ=255)                                                                                                                                       aGGCTTACATCGAGGGTGGAATACGTATTAGCGAACGCGTACAACGGCAGGTTCTCGTTCATTTTGCTAATTTCTTCGCACAGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGATCAGtttt              >  2:332926/1‑150 (MQ=255)                                                                                                                                            tACATCGAGGGTGGAATACGTATTAGCGAACGCGTACAACGGCAGGTTCTCGTTCATTTTGCTAATTTCTTCGCACAGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACTCGCATTGTTTTCGATCAGTTTtaataa         <  1:808490/150‑1 (MQ=255)                                                                                                                                                tCGAGGGTGGAATACGTATTAGCGAACGCGTACAACGGCAGGTTCTCGTTCATTTTGCTAATTTCTTCGCACAGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGATCAGTTTTAATAAgtcgt    >  2:454406/1‑151 (MQ=255)                                                                                                                                                  gaggGTGGAATACGTATTAGCGAACGCGTACAACGGCAGGTTCTCGTTCATTTTGCTAATTTCTTCGCACAGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGATCAGTTTTAATAAgtcgtc   >  1:56053/1‑150 (MQ=255)                                                                                                                                                  gaggGTGGAATACGTATTAGCGAACGCGTACAACGGCAGGTTCTCGTTCATTTTGCTAATTTCTTCGCACAGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGATCAGTTTTAATAAGTCGTCa  <  1:715641/151‑1 (MQ=255)                                                                                                                                                  gaggGTGGAATACGTATTAGCGAACGCGTACAACGGCAGGTTCTCGTTCATTTTGCTAATTTCTTCGCACAGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGATCAGTTTTAATAAGTCGTCa  <  2:499322/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    TAAACAGTGCTTTAGTCAGCGGAGGCAGAATAGTGTTGATATATTCGTCAGTGGTCTGCTTGATCTTATTAGCAATATCTTCAGCAGCACCCAGCGCATATTCAAAGAAGCTAATCTGTAAACGCAGGTCATTCAGGCTTACATCGAGAGTGGAATACGTATTAGCGAACGCGTACAACGGCAGGTTCTCGTTCATTTTGCTAATTTCTTCGCACAGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGATCAGTTTTAATAAGTCGTCA  >  NC_000913/4358206‑4358501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑