Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	514,375	G→A	G128D (GGC→GAC)	cueR →	copper‑responsive regulon transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	514,375	0	G	A	100.0%	204.8 / NA	54	G128D (GGC→GAC)	cueR	copper‑responsive regulon transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/29);  total (25/29)

GTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCG  >  NC_000913/514230‑514517                                                                                                                                                  |                                                                                                                                               gTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGactgct                                                                                                                                           >  1:843289/1‑151 (MQ=255) gTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGactgct                                                                                                                                           <  2:285797/151‑1 (MQ=255)   cAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGActgctg                                                                                                                                          <  1:901301/150‑1 (MQ=255)   cAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGt                                                                                                                                         <  2:719792/151‑1 (MQ=255)    aaaCGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGtc                                                                                                                                        <  1:511013/151‑1 (MQ=255)        ggCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATTCATGCGCGCCCAGCTGCTGGCACTGGCGAATGCCTTCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGtcatca                                                                                                                                    <  2:417930/151‑1 (MQ=255)             aCGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCggg                                                                                                                               >  2:418666/1‑151 (MQ=255)                cTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGc                                                                                                                              >  1:510642/1‑149 (MQ=255)                     gaAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGa                                                                                                                       <  2:184468/151‑1 (MQ=255)                        ggTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTa                                                                                                                    <  1:689020/151‑1 (MQ=255)                              ggAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAagacg                                                                                                               >  2:329487/1‑150 (MQ=255)                                agaTCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAagacgaga                                                                                                            <  2:423217/151‑1 (MQ=255)                                agaTCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAagacg                                                                                                               >  1:660879/1‑148 (MQ=255)                                agaTCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAagacg                                                                                                               >  2:179578/1‑148 (MQ=255)                                  aTCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAagacgagacg                                                                                                          >  1:437863/1‑151 (MQ=255)                                    cGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACgg                                                                                                         <  2:263400/150‑1 (MQ=255)                                     gAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGGTGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCt                                                                                                       <  2:307964/151‑1 (MQ=255)                                       aCGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCg                                                                                                     >  2:245853/1‑151 (MQ=255)                                        cGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCgg                                                                                                    >  1:588693/1‑151 (MQ=255)                                        cGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCgg                                                                                                    <  1:252375/151‑1 (MQ=255)                                         gACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGa                                                                                                   <  2:654173/151‑1 (MQ=255)                                          acacATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGat                                                                                                  >  2:516247/1‑151 (MQ=255)                                           cacaTTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGata                                                                                                 <  1:273769/151‑1 (MQ=255)                                            acaTTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGatat                                                                                                <  1:418711/151‑1 (MQ=255)                                                 gaggagCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAgg                                                                                           <  1:844229/151‑1 (MQ=255)                                                 gaggagCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAg                                                                                            <  1:449438/150‑1 (MQ=255)                                                   ggagCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGt                                                                                         >  1:782271/1‑151 (MQ=255)                                                      gCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTAt                                                                                      >  1:641465/1‑151 (MQ=255)                                                             tCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCt                                                                               >  2:828719/1‑151 (MQ=255)                                                              ccATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCt                                                                               <  2:906697/150‑1 (MQ=255)                                                               cATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTAt                                                                             >  1:9183/1‑151 (MQ=255)                                                               cATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTAt                                                                             <  1:170552/151‑1 (MQ=255)                                                                  gcgcGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCg                                                                           <  1:694754/150‑1 (MQ=255)                                                                   cgcgACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGc                                                                          >  2:734058/1‑150 (MQ=255)                                                                     cgACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAAt                                                                       >  2:745141/1‑151 (MQ=255)                                                                          aGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACtt                                                                  >  1:15258/1‑151 (MQ=255)                                                                          aGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACtt                                                                  <  1:894384/151‑1 (MQ=255)                                                                            ctgctgGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTc                                                                 >  1:735734/1‑150 (MQ=255)                                                                            ctgctgGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGAAGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACtt                                                                  >  2:113910/1‑149 (MQ=255)                                                                              gctgGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAAc                                                              <  1:726540/151‑1 (MQ=255)                                                                                      tGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCATCATGCGTAc                                                      >  1:689231/1‑151 (MQ=255)                                                                                           aaTGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGc                                                  >  1:634784/1‑150 (MQ=255)                                                                                           aaTGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCGCCGACTGCTGTCATCGTCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGc                                                  <  2:804460/150‑1 (MQ=255)                                                                                              gcctgccCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCg                                              >  1:617184/1‑151 (MQ=255)                                                                                               cctgccCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCga                                             <  2:707402/151‑1 (MQ=255)                                                                                                   cccTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGAt                                          <  2:161747/150‑1 (MQ=255)                                                                                                         cGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCt                                   >  2:477370/1‑151 (MQ=255)                                                                                                             gACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGgc                                <  1:411929/150‑1 (MQ=255)                                                                                                              aCAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGGCGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTGCAACATGCGTACCTGCACCGAGATCCTCGCt                                   <  2:637304/146‑1 (MQ=255)                                                                                                                    ccGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGAc                          >  2:864742/1‑149 (MQ=255)                                                                                                                     cGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACa                         <  2:469406/149‑1 (MQ=255)                                                                                                                      gACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGc                      >  1:373344/1‑151 (MQ=255)                                                                                                                                 tATCGAAAATCTCTCCGACTGCTGTCATCAGCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGt            <  2:779713/150‑1 (MQ=255)                                                                                                                                  aTCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCa          <  2:381097/151‑1 (MQ=255)                                                                                                                                    cGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCAcc        >  1:433788/1‑151 (MQ=255)                                                                                                                                             ctcCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGAcgcg  <  1:824087/148‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                               GTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCG  >  NC_000913/514230‑514517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑