Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,445,200	G→A	W363* (TGG→TGA)	tamB →	translocation and assembly module for autotransporter export, inner membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,445,200	0	G	A	100.0%	214.9 / NA	58	W363* (TGG→TGA)	tamB	translocation and assembly module for autotransporter export, inner membrane subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/23);  total (35/23)

AGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGCGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGGCCGTTCACTGGTGAGAAGCAGTATCAGGCGGATGATCTGAAACTGAAACTTACCGGCAAAATGACCGATTACACGCTCTCTATGCGTACGGCAGTGAAGGGACAGGAGATCCCGCCCGCGACTATTACCCTCGACGCCAAAGG  >  NC_000913/4445052‑4445343                                                                                                                                                     |                                                                                                                                                aGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGCGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGAcc                                                                                                                                               <  2:497041/151‑1 (MQ=255)  gCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGCGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAACGCGGATTGCCGCTCAACGTGGAAGTGAACAGCGAACAGCTTTACTGACCg                                                                                                                                              >  2:271091/1‑151 (MQ=255)            ggcggcGCGCTGCGCGAACAGCTGGAGATTGGCGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGACCGTTCACTGGTg                                                                                                                                    <  1:737836/151‑1 (MQ=255)              cggcgCGCTGCGCGAACAGCTGGAGTTTGGCGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGCCTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGACCGATCACTggcg                                                                                                                                    >  2:653535/1‑147 (MQ=255)                gcgcgcTGCGCGAACAGCTGGAGATTGGCGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGACCGTTCACTGGTGAGaa                                                                                                                                <  2:426635/151‑1 (MQ=255)                  gcgcTGCGCGAACAGCTGGAGATTGGCGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGACCGTTCACTGGTGAGAAGc                                                                                                                              >  1:907510/1‑151 (MQ=255)                      tGCGCGAACAGCTGGAGATTGGCGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGACCGTTCACTGGTGAGAAGCAGt                                                                                                                           >  1:13648/1‑150 (MQ=255)                       gcgcGAACAGCTGGAGATTGGCGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGACCGTTCACTGGTGAGAAGCAGTAt                                                                                                                         >  1:552019/1‑151 (MQ=255)                          cgAACAGCTGGAGATTGGCGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGACCGTTCACTGGTGAGAAGCAGTATc                                                                                                                        >  1:841545/1‑149 (MQ=255)                                 cTGGAGATTGGCGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGACCGTTCACTGGTGAGAAGCAGTATCAGGCGgatg                                                                                                               >  1:167636/1‑151 (MQ=255)                                       aTTGGCGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGACCGTTCACTGGTGAGAAGCAGTATCAGGCGGATGATctga                                                                                                         <  2:178673/151‑1 (MQ=255)                                          ggCGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGACCGTTCACTGGTGAGAAGCAGTATCAGGCGGATGATctgaaa                                                                                                       <  1:155963/150‑1 (MQ=255)                                                aaTCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGACCGTTCACTGGTGAGAAGCAGTATCAGGCGGATGATctgaaactgaaa                                                                                                 <  2:302778/150‑1 (MQ=255)                                                aaTCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGACCGTTCACTGGTGAGAAGCAGTATCAGGCGGATGATCtgaaactgaaac                                                                                                >  1:157998/1‑151 (MQ=255)                                                aaTCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGAACGTTCACTGGTGAGAAGCAGTATCAGGCGGATGATCtgaaactgaaac                                                                                                >  1:776359/1‑151 (MQ=255)                                                 aTCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGACCGTTCACTGGTGAGAAGCAGTATCAGGCGGATGATCTgaaactgaaact                                                                                               >  1:415084/1‑151 (MQ=255)                                                  tCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGACCGTTCACTGGTGAGAAGCAGTATCAGGCGGATGATCTGAAACTGAAACtt                                                                                              >  2:652341/1‑151 (MQ=255)                                                     ttCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGACCGTTCACTGGTGAGAAGCAGTATCAGGCGGATGATCTGAAACTGAAACtt                                                                                              <  1:286787/148‑1 (MQ=255)                                                      tccggtccggTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGACCGTTCACTGGTGAGAAGCAGTATCAGGCGGATGATCTGAAACTGAAACTTACCg                                                                                          >  2:84633/1‑151 (MQ=255)                                                      tccggtccggTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGACCGTTCACTGGTGAGAAGCAGTATCAGGCGGATGATCTGAAACTGAAACTTACCg                                                                                          >  1:899598/1‑151 (MQ=255)                                                         ggtccggtGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGACCGTTCACTGGTGAGAAGCAGTATCAGGCGGATGATCTGAAACTGAAACTTACCGGCa                                                                                       <  1:295237/151‑1 (MQ=255)                                                          gtccggtGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGACCGTTCACTGGTGAGAAGCAGTATCAGGCGGATGATCTGAAACTGAAACTTACCGGCaa                                                                                      <  1:389153/151‑1 (MQ=255)                                                              ggtGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGACCGTTCACTGGTGAGAAGCAGTATCAGGCGGATGATCTGAAACTGAAACTTACCGGCAAAATg                                                                                  <  1:117771/151‑1 (MQ=255)                                                               gtGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGACCGTTCACTGGTGAGAAGCAGTATCAGGCGGATGATCTGAAACTGAAACTTACCGGCAAAATg                                                                                  >  2:855111/1‑150 (MQ=255)                                                                tGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGACCGTTCACTGGTGAGAAGCAGTATCAGGCGGATGATCTGAAACTGAAACTTACCGGCAAAATGAc                                                                                >  1:433764/1‑151 (MQ=255)                                                                        gATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGACCGTTCACTGGTGAGAAGCAGTATCAGGCGGATGATCTGAAACTGAAACTTACCGGCAAAATGACCGATTac                                                                         >  1:561388/1‑150 (MQ=255)                                                                                cgcCCAGACGCGACTGGCGGAAGCCGGATTGCCGCCCAACGTGGACGTGAACAGCAAACAGCTTTACTGACCGTTCACTGGTGAGAAGCAGTATCAGGCGGATGATCTGAAACTGAAACTTACCGGCAAAATGACCGATTACACGccctct                                                                >  2:753682/1‑151 (MQ=255)                                                                                 gcCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGACCGTTCACTGGAGAGAAGCAGTATCAGGCGGATGATCTGAAACTGAAACTTACCGGCAAAATGACCGATTACACGctctc                                                                 >  2:547571/1‑149 (MQ=255)                                                                                    cAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGACCGTTCACTGGTGAGAAGCAGTATCAGGCGGATGATCTGAAACTGAAACTTACCGGCAAAATGACCGATTACACGCTCTCTATGc                                                            >  1:810958/1‑151 (MQ=255)                                                                                      gACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGACCGTTCACTGGTGAGAAGCAGTATCAGGCGGATGATCTGAAACTGAAACTTACCGGCAAAATGACCGATTACACGCTCTCTATGCGt                                                          <  1:307558/151‑1 (MQ=255)                                                                                       aCGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGACCGTTCACTGGTGAGAAGCAGTATCAGGCGGATGATCTGAAACTGAAACTTACCGGCAAAATGACCGATTACACGCTCTCTATGCGTa                                                         <  2:34541/151‑1 (MQ=255)                                                                                          cgACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACCGCTTTACTGACCGTTCACTGGTGAGAAGCAGTATCAGGCGGATGATCTGAAACTGAAACTTACCGGCAAAATGACCGATTACACGCTCTCTATGCGTACgg                                                      >  2:605025/1‑151 (MQ=255)                                                                                                    aaGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGACCGTTCACTGGTGAGAAGCAGTATCAGGCGGATGATCTGAAACTGAAACTTACCGGCAAAATGACCGATTACACGCTCTCTATGCGTACGGCAGTGAAggg                                            <  2:565527/151‑1 (MQ=255)                                                                                                       ccGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGACCGTTCACTGGTGAGAAGCAGTATCAGGCGGATGATCTGAAACTGAAACTTACCGGCAAAATGACCGATTACACGCTCTCTATGCGTACGGCAGTGAAGGGAc                                          >  2:571680/1‑150 (MQ=255)                                                                                                         ggATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGACCGTTCACTGGTGAGAAGCTGTATCAGGCGGATGATCTGAAACTGAAACTTACCGGCAAAATGACCGATTACACGCTCTCTATGCGTACGGCAGTGAAGGGACAgg                                       >  1:229830/1‑151 (MQ=255)                                                                                                         ggATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGACCGTTCACTGGTGAGAAGCAGTATCAGGCGGATGATCTGAAACTGAAACTTACCGGCAAAATGACCGATTACACGCTCTCTATGCGTACGGCAGTGAAGGGACAgg                                       <  2:586026/151‑1 (MQ=255)                                                                                                         ggATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGACCGTTCACTGGTGAGAAGCAGTATCAGGCGGATGATCTGAAACTGAAACTTACCGGCAAAATGACCGATTACACGCTCTCTATGCGTACGGCAGTGAAGGGACAgg                                       <  1:165826/151‑1 (MQ=255)                                                                                                         ggATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGACCGTTCACTGGTGAGAAGCAGTATCAGGCGGATGATCTGAAACTGAAACTTACCGGCAAAATGACCGATTACACGCTCTCTATGCGTACGGCAGTGAAGGGACAgg                                       >  1:268878/1‑151 (MQ=255)                                                                                                          gATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGACCGTTCACTGGTGAGAAGCAGTATCAGGCGGATGATCTGAAACTGAAACTTACCGGCAAAATGACCGATTACACGCTCTCTATGCGTACGGCAGTGAAGGGACAgg                                       >  1:522931/1‑150 (MQ=255)                                                                                                              gCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGACCGTTCACTGGTGAGAAGCAGTATCAGGCGGATGATCTGAAACTGAAACTTACCGGCAAAATGACCGATTACACGCTCTCTATGCGTACGGCAGTGAAGGGACAGGAGAt                                   <  1:345653/150‑1 (MQ=255)                                                                                                                cGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGACCGTTCACTGGTGAGAAGCAGTATCAGGCGGATGATCTGAAACTGAAACTTACCGGCAAAATGACCGATTACACGCTCTCTATGCGTACGGCAGTGAAGGGACAGGAGATccc                                >  2:186661/1‑151 (MQ=255)                                                                                                                cGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGACCGTTCACTGGTGAGAAGCAGTATCAGGCGGATGATCTGAAACTGAAACTTACCGGCAAAATGACCGATTACACGCTCTCTATGCGTACGGCAGTGAAGGGACAGGAGATccc                                <  2:338080/151‑1 (MQ=255)                                                                                                                cGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGACCGTTCACTGGTGAGAAGCAGTATCAGGCGGATGATCTGAAACTGAAACTTACCGGCAAAATGACCGATTACACGCTCTCTATGCGTACGGCAGTGAAGGGACAGGAGATccc                                >  1:685254/1‑151 (MQ=255)                                                                                                                cGCCCAACGTGGACGTGACCAGCCAACAGCTTTACTCACCGTTCACTGGTGAGAAGCAGTATCAGGAGGACGATCTGAAACTGATACTTACCGGCAAATTTACCGATTACCCTCTCTCTATGCGTACTGCAGTGAAGGGACAGGCGATc                                  <  2:130803/149‑1 (MQ=255)                                                                                                                          ggAAGTGAACAGCAAACAGCTTTACTGACCGTTCACTGGTGAGAAGCAGTATCAGGCGGATGATCTGAAACTGAAACTTACCGGCAAAATGACCGATTACACGCTCTCTATGCGTACGGCAGTGAAGGGACAGGAGATCCCGCCCGCGACt                      >  1:152578/1‑151 (MQ=255)                                                                                                                            aaGTGAACAGCAAACAGCTTTACTGACCGTTCACTGGTGAGAAGCAGTATCAGGCGGATGATCTGAAACTGAAACTTACCGGCAAAATGACCGATTACACGCTCTCTATGCGTACGGCAGTGAAGGGACAGGAGATCCCGCCCGCGACTAt                    >  1:65745/1‑151 (MQ=255)                                                                                                                              gTGAACAGCAAACAGCTTTACTGACCGTTCACTGGTGAGAAGCAGTATCAGGCGGATGATCTGAAACTGAAACTTACCGGCAAAATGACCGATTACACGCTCTCTATGCGTACGGCAGTGAAGGGACAGGAGATCCCGCCCGCGACTATTa                  <  2:514083/151‑1 (MQ=255)                                                                                                                               tGAACAGCAAACAGCTTTACTGACCGTTCACTGGTGAGAAGCAGTATCAGGCGGATGATCTGAAACTGAAACTTACCGGCAAAATGACCGATTACACGCTCTCTATGCGTACGGCAGTGAAGGGACAGGAGATCCCGCCCGCGACTATTAc                 >  2:418822/1‑151 (MQ=255)                                                                                                                                gAACAGCAAACAGCTTTACTGACCGTTCACTGGTGAGAAGCAGTATCAGGCGGATGATCTGAAACTGAAACTTACCGGCAAAATGACCGATTACACGCTCTCTATGCGTACGGCAGTGAAGGGACAGGAGATCCCGCCCGCGACTATTAc                 <  1:108581/150‑1 (MQ=255)                                                                                                                                gAACAGCAAACAGCTTTACTGACCGTTCACTGGTGAGAAGCAGTATCAGGCGGATGATCTGAAACTGAAACTTACCGGCAAAATGACCGATTACACGCTCTCTATGCGTACGGCAGTGAAGGGACAGGAGATCCCGCCCGCGACTATTAc                 <  1:716590/150‑1 (MQ=255)                                                                                                                                     gCAAACAGCTTTACTGACCGTTCACTGGTGAGAAGCAGTATCAGGCGGATGATCTGAAACTGAAACTTACCGGCAAAATGACCGATTACACGCTCTCTATGCGTACGGCAGTGAAGGGACAGGAGATCCCGCCCGCGACTATTACCCTCGa           <  1:602214/151‑1 (MQ=255)                                                                                                                                          cAGCTTTACTGACCGTTCACTGGTGAGAAGCAGTATCAGGCGGATGATCTGAAACTGAAACTTACCGGCAAAATGACCGATTACACGCTCTCTATGCGTACGGCAGTGAAGGGACAGGAGATCCCGCCCGCGACTATTACCCTCGACGcc       >  2:855348/1‑150 (MQ=255)                                                                                                                                            gCTTTACTGACCGTTCACTGGTGAGAAGCAGTATCAGGCGGATGATCTGAAACTGAAACTTACCGGCAAAATGACCGATTACACGCTCTCTATGCGTACGGCAGTGAAGGGACAGGAGATCCCGCCCGCGACTATTACCCTCGACGCCaa     >  1:693342/1‑150 (MQ=255)                                                                                                                                             cTTTACTGACCGTTCACTGGTGAGAAGCAGTATCAGGCGGATGATCTGAAACTGAAACTTACCGGCAAAATGACCGATTACACGCTCTCTATGCGTACGGCAGTGAAGGGACAGGAGATCCCGCCCGCGACTATTACCCTCGACGCCaa     >  2:186604/1‑149 (MQ=255)                                                                                                                                             cTTTACTGACCGTTCACTGGTGAGAAGCAGTATCAGGCGGATGATCTGAAACTGAAACTTACCGGCAAAATGACCGATTACACGCTCTCTATGCGTACGGCAGTGAAGGGACAGGAGATCCCGCCCGCGACTATTACCCTCGACGCCaa     >  1:6361/1‑149 (MQ=255)                                                                                                                                              tttACTGACCGTTCACTGGTGAGAAGCAGTATCAGGCGGATGATCTGAAACTGAAACTTACCGGCAAAATGACCGATTACACGCTCTCTATGCGTACGGCAGTGAAGGGACAGGAGATcccgcccg                           <  1:243030/126‑1 (MQ=255)                                                                                                                                              tttACTGACCGTTCACTGGTGAGAAGCAGTATCAGGCGGATGATCTGAAACTGAAACTTACCGGCAAAATGACCGATTACACGCTCTCTATGCGTACGGCAGTGAAGGGACAGGAGATcccgcccg                           >  2:243004/1‑126 (MQ=255)                                                                                                                                              tttACTGACCGTTCACTGGTGAGAAGCAGTATCAGGCGGATGATCTGAAACTGAAACTTACCGGCAAAATGACCGATTACACGCTCTCTATGCGTACGGCAGTGAAGGGACAGGAGATCCCGCCCGCGACTATTACCCTCGACGCCAAAgg  >  1:20882/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                AGCTGAAAATGGGCGGCGCGCTGCGCGAACAGCTGGAGATTGGCGTTAATCTTTCCGGTCCGGTGGATATGGATTTACGCGCCCAGACGCGACTGGCGGAAGCCGGATTGCCGCTCAACGTGGAAGTGAACAGCAAACAGCTTTACTGGCCGTTCACTGGTGAGAAGCAGTATCAGGCGGATGATCTGAAACTGAAACTTACCGGCAAAATGACCGATTACACGCTCTCTATGCGTACGGCAGTGAAGGGACAGGAGATCCCGCCCGCGACTATTACCCTCGACGCCAAAGG  >  NC_000913/4445052‑4445343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑