Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	523,968	C→T	G236G (GGC→GGT)	rhsD →	Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	523,968	0	C	T	100.0%	153.6 / NA	48	G236G (GGC→GGT)	rhsD	Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/27);  total (21/27)

TGAGCGGGTGCCGGGTGCTGAGGACGTACTGCCAGCGCCGCTGCCGCCGTACCGGGTGCTTACCGGGATGGCGGACCGCTTCGGGCGGACGCTGACGTACCGGCGTGAGGCCGCCGGTGACCTGGCCGGGGAAATCACCGGCGTGACGGACGGTGCCGGGCGGGAGTTCCGTCTGGTGCTGACCACGCAGGCGCAGCGTGCGGAAGAGGCCCGCACCTCTTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGT  >  NC_000913/523827‑524112                                                                                                                                              |                                                                                                                                                 tGAGCGGGTGCCGGGTGCTGAGGACGTACTGCCAGCGCCGCTGCCGCCGTACCGGGTGCTTACCGGGATGGCGGACCGCTTCGGGCGGACGCTGACGTACCGGCGTGAGGCCGCCGGTGACCTGGCCGGGGAAATCACCGGTGTgacgga                                                                                                                                          >  1:700172/1‑150 (MQ=255)  gAGCGGGTGCGGGGTGCTGAGGACGTACTGCCAGCGCCGCTGCCGCCGTACCGGGTGCTTACCGGGATGGCGGACCGCTTCGGGCGGACGCTGACGTACCGGCGTGAGGCCGCCGGTGACCTGGCCGGGGAAATCACCGGTGTgacggacg                                                                                                                                        <  2:649444/151‑1 (MQ=255)  gAGCGGGTGCCGGGTGCTGAGGACGTACTGCCAGCGCCGCTGCCGCCGTACCGGGTGCTTACCGGGATGGCGGACCGCTTCGGGCGGACGCTGACGTACCGGCGTGAGGCCGCCGGTGACCTGGCCGGGGAAATCACCGGTGTgacgg                                                                                                                                           <  2:34845/148‑1 (MQ=255)     cgggtgccgggtgcTGAGGACGTACTGCCAGCGCCGCTGCCGCCGTACCGGGTGCTTACCGGGATGGCGGACCGCTTCGGGCGGACGCTGACGTACCGGCGTGAGGCCGCCGGTGACCTGGCCGGGGAAATCACCGGTGTGACGGACGGt                                                                                                                                      >  2:589736/1‑150 (MQ=255)      gggtgccgggtgcTGAGGACGTCCTGCCAGCGCCGCTGCCGCCGTACCGGGTGCTTACCGGGATGGCGGAGCGCTTCGGGCCGACGCTGACGTACCGGCGTGAGGCCGCCGGTGACCTGGCCGGGGAAATCACCGGTGTgacggacg                                                                                                                                        <  1:522539/147‑1 (MQ=255)             gggtgcTGAGGACGTACTGCCAGCGCCGCTGCCGCCGTACCGGGTGCTTACCGGGATGGCGGACCGCTTCGGGCGGACGCTGACGTACCGGCGTGAGGCCGCCGGTGACCTGGCCGGGGAAATCACCGGTGTGACGGACGGGGCcgggcgg                                                                                                                             >  2:711854/1‑151 (MQ=255)              ggtgcTGAGGACGTACGGCCAGCGCCGCTGCCGCCGTACCGGGTGCTTACCGGGATGGCGGACCGCTTCGGGCGGACGCTGACGGACCGGCGTGAGGCCGCCGGTGACCTGGCCGGGGAAATCACCGGTGTGACGGACGGTGCcgggcggg                                                                                                                            <  2:592295/151‑1 (MQ=255)                    gAGGACGTACTGCCAGCGCCGCTGCCGCCGTACCGGGTGCTTACCGGGATGGCGGACCGCTTCGGGCGGACGCTGACGTACCGGCGTGAGGCCGCCGGTGACCTGGCCGGGGAAATCACCGGTGTGACGGACGGTGCCGGGCGGGAGTTcc                                                                                                                      <  2:551784/151‑1 (MQ=255)                      gggcGTACTGCCAGCGCCGCTGCCGCCGTACCGGGTGCTTACCGGGATGGCGGACCGCTTCGGGCGGACGCTGACGTACCGGCGTGAGGCCGCCGGTGACCTGGCCGGGGAAATCACCGGTGTGACGGACGGTGCCGGGCGGGAGTTcc                                                                                                                      <  2:3916/146‑1 (MQ=255)                         cGTACTGCCAGCGCCGCTGCCGCCGTACCGGGTGCTTACCGGGATGGCGGACCGCTTCGGGCGGACGCTGACGTACCGGCGTGAGGCCGCCGGTGACCTGGCCGGGGAAATCACCGGTGTGACGGACGGTGCCGGGCGGGAGTTCCGTCTg                                                                                                                 >  2:203085/1‑151 (MQ=255)                                 cAGCGCCGCTGCCGCCGTACCGGGTGCTTACCGGGATGGCGGACCGCTTCGGGCGGACGCTGACGTACCGGCGTGAGGCCGCCGGTGACCTGGCCGGGGAAATCACCGGTGTGACGGACGGTGCCGGGCGGGAGTTCCGTCTGGTGCTGAc                                                                                                         >  2:551350/1‑151 (MQ=255)                                        gcTGCCGCCGTACCGGGTGCTTACCGGGATGGCGGACCGCTTCGGGCGGACGCTGACGTACCGGCGTGAGGCCGCCGGTGACCTGGCCGGGGAAATCACCGGTGTGACGGACGGTGCCGGGCGGGAGTTCCGTCTGGTGCTGACCACGCAg                                                                                                  <  2:898813/151‑1 (MQ=255)                                         cTGCCGCCGTACCGGGTGCTTACCGGGATGGCGGACCGCTTCGGGCGGACGCTGACGTACCGGCGTGAGGCCGCCGGTGACCTGGCCGGGGAAATCACCGGTGTGACGGACGGTGCCGGGCGGGAGTTCCGTCTGGTGCTGACCACGCAg                                                                                                  <  1:228275/150‑1 (MQ=255)                                           gccgccGTACCGGGTGCTTACCGGGATGGCGGACCGCTTCGGGCGGACGCTGACGTACCGGCGTGAGGCCGCCGGTGACCTGGCCGGGGAAATCACCGGTGTGACGGACGGTGCCGGGCGGGAGTTCCGTCTGGTGCTGACCACGCAGgc                                                                                                >  2:362714/1‑150 (MQ=255)                                            ccgccgTACCGGGTGCTTACCGGGATGGCGGACCGCTTCGGGCGGACGCTGACGTACCGGCGTGAGGCCGCCGGTGACCTGGCCGGGGAAATCACCGGTGTGACGGACGGTGCCGGGCGGGAGTTCCGTCTGGTGCTGACCACGCAgg                                                                                                 <  1:270750/148‑1 (MQ=255)                                              gccgTACCGGGTGCTTACCGGGATGGCGGACCGCTTCGGGCGGACGCTGACGTACCGGCGTGAGGCCGCCGGTGACCTGGCCGGGGAAATCACCGGTGTGACGGACGGTGCCGGGCGGGAGTTCCGTCTGGTGCTGACCACGCAGGCGCAg                                                                                            <  2:151689/151‑1 (MQ=255)                                                cgTACCGGGTGCTTACCGGGATGGCGGACCGCTTCGGGCGGACGCTGACGTCCCGGCGTGAGGCCGCCGGTGACCTGGCCGGGGAAATCACCGGTGTGACGGACGGTGCCGGGCGGGAGTTCCGTCTGGTGCTGACCACGCAGGCGCAGCg                                                                                          <  2:768215/151‑1 (MQ=255)                                                    ccGGGTGCTTACCGGGATGGCGGACCGCTTCGGGCGGACGCTGACGTACCGGCGTGAGGCCGCCGGTGACCTGGCCGGGGAAATCACCGGTGTGACGGACGGTGCCGGGCGGGAGTTCCGTCTGGTGCTGACCACGCAGGCGCAGCGTGCg                                                                                      <  1:697695/151‑1 (MQ=255)                                                      gggTGCTTACCGGGATGGCGGACCGCTTCGGGCGGACGCTGACGTACCGGCGTGAGGCCGCCGGTGACCTGGCCGGGGAAATCACCGGTGTGACGGACGGTGCCGGGCGGGAGTTCCGTCTGGTGCTGACCACGCAGGCGCAGCGTGCgg                                                                                     >  2:878057/1‑150 (MQ=255)                                                        gTGCTTACCGGGATGGCGGACCGCTTCGGGCGGACGCTGACGTACCGGCGTGAGGCCGCCGGTGACCTGGCCGGGGAAATCACCGGTGTGACGGACGGTGCCGGGCGGGAGTTCCGTCTGGTGCTGACCACGCAGGCGCAGCGTGCgg                                                                                     >  2:638595/1‑148 (MQ=255)                                                           cTTACCGGGATGGCGGACCGCTTCGGGCGGACGCTGACGTACCGGCGTGAGGCCGCCGGTGACCTGGCCGGGGAAATCACCGGTGTGACGGACGGTGCCGGGCGGGAGTTCCGTCTGGTGCTGACCACGCAGGCGCAGCGTGCGGAAGAgg                                                                               >  1:611084/1‑151 (MQ=255)                                                              aCCGGGATGGCGGACCGCTTCGGGCGGACGCTGACGTACCGGCGTGAGGCCGCCGGTGACCTGGCCGGGGAAATCACCGGTGTGACGGACGGTGCCGGGCGGGAGTTCCGTCTGGTGCTGACCACGCAGGCGCAGCGTGCGGAAGAGGccc                                                                            <  2:141437/151‑1 (MQ=255)                                                               ccGGGATGGCGGGCCGCTTCGGGCGGACGCTGACGTACCGGCGTGAGGCCGCCGGTGACCTGGCCGGGGAAATCACCGGTGTGACGGACGGTGCCGGGCGGGAGTTCCGTCTGGTGCTGACCACGCAGGCGCAGCGTGCGGAAGAGGccc                                                                            <  1:729823/150‑1 (MQ=255)                                                                          gACCGCTTCGGGCGGACGCTGACGTACCGGCGTGAGGCCGCCGGTGACCTGGCCGGGGAAATCACCGGTGTGACGGACGGTGCCGGGCGGGAGTTCCGTCTGGTGCTGACCACGCAGGCGCAGCGTGCGGAAGAGGCCCGCACCTCTTCGc                                                                <  2:202747/151‑1 (MQ=255)                                                                            ccGCTTCGGGCGGACGCTGACGTACCGGCGTGAGGCCGCCGGTGACCTGGCCGGGGAAATCACCGGTGTGACGGACGGTGCCGGGCGGGAGTTCCGTCTGGTGCTGACCACGCAGGCGCAGCGTGCGGAAGAGGCCCGCACCTCTTCGCTa                                                              <  1:140480/151‑1 (MQ=255)                                                                              gCTTCGGGCGGACGCTGACGTACCGGCGTGAGGCCGCCGGTGACCTGGCCGGGGAAATCACCGGTGTGACGGACGGTGCCGGGCGGGAGTTCCGTCTGGTGCTGACCACGCAGGCGCAGCGTGCGGAAGAGGCCCGCACCTCTTCGCTAtc                                                            >  2:664213/1‑151 (MQ=255)                                                                                 tCGGGCGGACGCTGACGTACCGGCGTGAGGCCGCCGGTGACCTGGCCGGGGAAATCACCGGTGTGACGGACGGTGCCGGGCGGGAGTTCCGTCTGGTGCTGACCACGCAGGCGCAGCGTGCGGAAGAGGCCCGCACCTCTTCGCTAtcttc                                                         >  2:269334/1‑151 (MQ=255)                                                                                  cGGGCGGACGCTGACGTACCGGCGTGAGGCCGCCGGTGACCTGGCCGGGGAAATCACCGGTGTGACGGACGGTGCCGGGCGGGAGTTCCGTCTGGTGCTGACCACGCAGGCGCAGCGTGCGGAAGAGGCCCGCACCTCTTCGCTAtcttct                                                        >  1:306008/1‑151 (MQ=255)                                                                                  cGGGCGGACGCTGACGTACCGGCGTGAGGCCGCCGGTGACCTGGCCGGGGAAATCACCGGTGTGACGGACGGTGCCGGGCGGGAGTTCCGTCTGGTGCTGACCACGCAGGCGCAGCGTGCGGAAGAGGCCCGCACCTCTTCGCTAtattct                                                        >  1:707388/1‑151 (MQ=255)                                                                                   gggCGGACGCTGACGTACCGGCGTGAGGCCGCCGGTGACCTGGCCGGGGAAATCACCGGTGTGACGGACGGTGCCGGGCGGGAGTTCCGTCTGGTGCTGACCACGCAGGCGCAGCGTGCGGAAGAGGCCCGCACCTCTTCGCTAtcttctt                                                       >  1:289967/1‑151 (MQ=255)                                                                                          cGCTGACGTACCGGCGTGAGGCCGCCGGTGACCTGGCCGGGGAAATCACCGGTGTGACGGACGGTGCCGGGCGGGAGTTCCGTCTGGTGCTGACCACGCAGGCGCAGCGTGCGGAAGAGGCCCGCACCTCTTCGCTATCTTCTTCTGACAg                                                <  2:732360/151‑1 (MQ=255)                                                                                               aCGTACCGGCGTGAGGCCGCCGGTGACCTGGCCGGGGAAATCACCGGTGTGACGGACGGTGCCGGGCGGGAGTTCCGTCTGGTGCTGACCCCGCAGGCGCAGCGTGCGGAAGAGGCCCGCACGTCTTCGCTATCTTCTTCTGACAGTTccc                                           >  2:317149/1‑151 (MQ=255)                                                                                                cGTACCGGCGTGAGGCCGCCGGTGACCTGGCCGGGGAAATCACCGGTGTGACGGACGGTGCCGGGCGGGAGTTCCGTCTGGTGCTGACCACGCAGGCGCAGCGTGCGGAAGAGGCCCGCACCTCTTCGCTATCTTCTTCTGACAGTTCCCg                                          <  2:23087/151‑1 (MQ=255)                                                                                                   aCCGGCGTGAGGCCGCCGGTGACCTGGCCGGGGAAATCACCGGTGTGACGGACGGTGCCGGGCGGGAGTTCCGTCTGGTGCTGACCACGCAGGCGCAGCGTGCGGAAGAGGCCCGCACCTCTTCGCTATCTTCTTCTGACAGTTCCCGccc                                       >  1:707045/1‑151 (MQ=255)                                                                                                    ccGGCGTGAGGCCGCCGGTGACCTGGCCGGGGAAATCACCGGTGTGACGGACGGTGCCGGGCGGGAGTTCCGTCTGGTGCTGACCACGCAGGCGCAGCGTGCGGAAGAGGCCCGCACCTCTTCGCTATCTTCTTCTGACAGTTCCCGcc                                        <  1:415520/149‑1 (MQ=255)                                                                                                           gAGGCCGCCGGTGACCTGGCCGGGGAAATCACCGGTGTGACGGACGGTGCCGGGCGGGAGTTCCGTCTGGTGCTGACCACGCAGGCGCAGCGTGCGGAAGAGGCCCGCACCTCTTCGCTATCTTCTTCTGACAGTTCCCGCCCtctctc                                 >  2:906314/1‑149 (MQ=255)                                                                                                           gAGGCCGCCGGTGACCTGGCCGGGGAAATCACCGGTGTGACGGACGGTGCCGGGCGGGAGTTCCGTCTGGTGCTGACCACGCAGGCGCAGCGTGCGGAAGAGGCCCGCACCTCTTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTctcag                               <  2:22704/151‑1 (MQ=255)                                                                                                            aGGCCGCCGGTGACCTGGCCGGGGAAATCACCGGTGTGACGGACGGTGCCGGGCGGGAGTTCCGTCTGGTGCTGACCACGCAGGCGCAGCGTGCGGAAGAGGCCCGCACCTCTTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTctcagc                              >  2:485808/1‑151 (MQ=255)                                                                                                             ggCCGCCGGTGACCTGGCCGGGGAAATCACCGGTGTGACGGACGGTGCCGGGCGGGAGTTCCGTCTGGTGCTGACCACGCAGGCGCAGCGTGCGGAAGAGGCCCGCACCTCTTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTctcagcc                             >  1:157657/1‑151 (MQ=255)                                                                                                                 gccgGTGACCTGGCCGGGGAAATCACCGGTGTGACGGACGGTGCCGGGCGGGAGTTCCGTCTGGTGCTGACCACGCAGGCGCAGCGTGCGGAAGAGGCCCGCACCTCTTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTctcagcct                            >  2:200165/1‑148 (MQ=255)                                                                                                                   cgGTGACCTGGCCGGGGAAATCACCGGTGTGACGGACGGTGCCGGGCGGGAGTTCCGTCTGGTGCTGACCACGCAGGCGCAGCGTGCGGAAGAGGCCCGCACCTCTTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTCTCAGCCTCAGCg                       <  2:294925/151‑1 (MQ=255)                                                                                                                    ggTGACCTGGCCGGGGAAATCACCGGTGTGACGGACGGTGCCGGGCGGGAGTTCCGTCTGGTGCTGACCACGCAGGCGCAGCGTGCGGAAGAGGCCCGCACCTCTTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTCTCAGCCTCAGCGt                      >  1:598157/1‑151 (MQ=255)                                                                                                                     gTGACCTGGCCGGGGAAATCACCGGTGTGACGGACGGTGCCGGGCGGGAGTTCCGTCTGGTGCTGACCACGCAGGCGCAGCGTGCGGAAGAGGCCCGCACCTCTTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTCTCAGCCTCAGCGtt                     <  2:162638/151‑1 (MQ=255)                                                                                                                               cGGGGAAATCACCGGTGTGACGGACGGTGCCGGGCGGGAGTTCCGTCTGGTGCTGACCACGCAGGCGCCGCGTGCGGAAGAGGCCCGCACCTCTTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGacac            <  2:16919/150‑1 (MQ=255)                                                                                                                                ggggAAATCACCGGTGTGACGGACGGTGCCGGGCGGGAGTTCCGTCTGGTGCTGACCACGCAGGCGCAGCGTGCGGAAGAGGCCCGCACCTCTTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGacaca           <  2:745439/150‑1 (MQ=255)                                                                                                                                 gggAAATCACCGGTGTGACGGACGGTGCCGGGCGGGAGTTCCGTCTGGTGCTGACCACGCAGGCGCCGCGTGCGGAAGAGGCCCGCACCTCTTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACt         <  2:568457/151‑1 (MQ=255)                                                                                                                                     aaTCACCGGTGTGACGGACGGTGCCGGGCGGGAGTTCCGTCTGGTGCTGACCACGCAGGCGCAGCGTGCGGAAGAGGCCCGCACCTCTTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGccc     <  1:260520/151‑1 (MQ=255)                                                                                                                                        cACCGGTGTGACGGACGGTGCCGGGCGGGAGTTCCGTCTGGTGCTGACCACGCAGGCGCAGCGTGCGGAAGAGGCCCGCACCTCTTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGt  <  2:611035/151‑1 (MQ=255)                                                                                                                                              |                                                                                                                                                 TGAGCGGGTGCCGGGTGCTGAGGACGTACTGCCAGCGCCGCTGCCGCCGTACCGGGTGCTTACCGGGATGGCGGACCGCTTCGGGCGGACGCTGACGTACCGGCGTGAGGCCGCCGGTGACCTGGCCGGGGAAATCACCGGCGTGACGGACGGTGCCGGGCGGGAGTTCCGTCTGGTGCTGACCACGCAGGCGCAGCGTGCGGAAGAGGCCCGCACCTCTTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGT  >  NC_000913/523827‑524112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑