Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	97,947	T→C	R287R (CGT→CGC)	murD →	UDP‑N‑acetylmuramoyl‑L‑alanine:D‑glutamate ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	97,947	0	T	C	100.0%	156.5 / NA	46	R287R (CGT→CGC)	murD	UDP‑N‑acetylmuramoyl‑L‑alanine:D‑glutamate ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/18);  total (28/18)

GAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTCCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGTGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCATAACGGCGTACGTTGGATTAACGATTCGAAAGCGACCAACGTCGGCAGTACGGAAGCGGCGCTGAATGGCCTGCACG  >  NC_000913/97806‑98092                                                                                                                                              |                                                                                                                                                  gAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTCCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGcc                                                                                                                                                >  2:704371/1‑145 (MQ=255) gAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTCCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGcc                                                                                                                                                <  1:704420/145‑1 (MQ=255) gAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTCCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCagcagc                                                                                                                                          <  1:766832/151‑1 (MQ=255)        cagcagGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTCCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGaa                                                                                                                                     >  2:565822/1‑149 (MQ=255)                    aCCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTCCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACAt                                                                                                                       >  2:825733/1‑151 (MQ=255)                            gCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTCCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTgg                                                                                                                <  2:131903/150‑1 (MQ=255)                             cGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTCCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTc                                                                                                              >  1:303999/1‑151 (MQ=255)                               ggTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTCCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTg                                                                                                            <  2:517264/151‑1 (MQ=255)                                gTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTCCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGc                                                                                                           >  1:263722/1‑151 (MQ=255)                                 ttAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTCCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGcc                                                                                                          >  1:637525/1‑151 (MQ=255)                                    aaGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTCCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGc                                                                                                        >  1:706901/1‑150 (MQ=255)                                     aGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTCCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCAt                                                                                                      <  1:419654/151‑1 (MQ=255)                                             aaGTGCTGAATGTGAAAGAGATGAAACTTTCCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTg                                                                                               >  1:282806/1‑150 (MQ=255)                                              aGTGCTGAATGTGAAAGAGATGAAACTTTCCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGaa                                                                                             <  1:230396/151‑1 (MQ=255)                                                 gCTGAATGTGAAAGAGATGAAACTTTCCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGtt                                                                                          >  2:353653/1‑151 (MQ=255)                                                 gCTGAATGTGAAAGAGATGAAACTTTCCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGt                                                                                           <  2:696462/150‑1 (MQ=255)                                                          gAAAGAGATGAAACTTTCCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGa                                                                                  <  2:724913/150‑1 (MQ=255)                                                          gAAAGAGATGAAACTTTCCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGa                                                                                  <  2:714095/150‑1 (MQ=255)                                                              gagaTGAAACTTTCCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCATa                                                                             <  2:320213/151‑1 (MQ=255)                                                                 aTGAAACTTTCCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCATAACg                                                                          >  2:352085/1‑151 (MQ=255)                                                                 aTGAAACTTTCCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCATAACg                                                                          <  2:509799/151‑1 (MQ=255)                                                                      aCTTTCCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCCCTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCATAACGGCg                                                                       >  2:641112/1‑149 (MQ=255)                                                                       cTTTCCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCATAACGGCGTAc                                                                    >  2:198958/1‑151 (MQ=255)                                                                       cTTTCCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGAGGCTGCAGGGGTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCGTAACGGCGt                                                                      >  2:161321/1‑149 (MQ=255)                                                                           ccGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCATAACGGCGTACGtt                                                                 <  1:465644/150‑1 (MQ=255)                                                                            cGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCATAACGGCGTACGTTgg                                                               <  1:815750/151‑1 (MQ=255)                                                                              ggCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCATAACGGCGTACGTTGGAt                                                             <  2:479984/151‑1 (MQ=255)                                                                                     tAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCATAACGGCGTACGTTGGATTAACGAt                                                      >  2:23037/1‑151 (MQ=255)                                                                                      aaCTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCATAACGGCGTACGTTGGATTAACGAtt                                                     >  2:582718/1‑151 (MQ=255)                                                                                      aaCTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCATAACGGCGTACGTTGGATTAACGAtt                                                     >  1:427219/1‑151 (MQ=255)                                                                                          acacCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCATAACGGCGTACGTTGGATTAACGATTCg                                                   <  1:829295/149‑1 (MQ=255)                                                                                          acacCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCATAACGGCGTACGTTGGATTAACGATTCGa                                                  >  1:788912/1‑150 (MQ=255)                                                                                            acCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCATAACGGCGTACGTTGGATTAACGATTCGaaa                                                <  2:632428/150‑1 (MQ=255)                                                                                                   cgctggcggcgctggcgCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCATAACGGCGTACGTTGGATTAACGATTCGAAAGCGACCa                                         >  1:468093/1‑150 (MQ=255)                                                                                                      tggcggcgctggcgCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCATAACGGCGTACGTTGGATTAACGATTCGAAAGCGACCAAc                                       <  2:877175/149‑1 (MQ=255)                                                                                                      tggcggcgctggcgCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCATAACGGCGTACGTTGGATTAACGATTCGAAAGCGACCAACg                                      >  2:901567/1‑150 (MQ=255)                                                                                                      tggcggcgctggcgCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCATAACGGCGTACGTTGGATTAACGATTCGAAAGCGACCAACGt                                     >  2:521775/1‑151 (MQ=255)                                                                                                      tggcggcgctggcgCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCATAACGGCGTACGTTGGATTAACGATTCGAAAGCGACCAACGt                                     >  1:660900/1‑151 (MQ=255)                                                                                                          ggcgctggcgctGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCATAACGGCGTACGTTGGATTAACGATTCGAAAGCGACCAACGTCGGc                                 >  2:147310/1‑151 (MQ=255)                                                                                                          ggcgctggcgctGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCATAACGGCGTACGTTGGATTAACGATTCGAAAGCGACCAACGTCGGc                                 >  1:875324/1‑151 (MQ=255)                                                                                                               tggcgctggcAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCATAACGGCGTACGTTGGATTAACGATTCGAAAGCGACCAACGTCGGCAGTAc                            >  1:885076/1‑151 (MQ=255)                                                                                                                ggcgctggcAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCATAACGGCGTACGTTGGATTAACGATTCGAAAGCGACCAACGTCGGCAGTACg                           >  2:785338/1‑151 (MQ=255)                                                                                                                    ctggcAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCATAACGGCGTACGTTGGATTAACGATTCGAAAGCGACCAACGTCGGCAGTACGGa                         >  2:362404/1‑149 (MQ=255)                                                                                                                    ctggcAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCATAACGGCGTACGTTGGATTAACGATTCGAAAGCGACCAACGTCGGCAGTACGGa                         >  1:241251/1‑149 (MQ=255)                                                                                                                     tggcAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCATAACGGCGTACGTTGGATTAACGATTCGAAAGCGACCAACGTCGGCAGTACGGAAgc                      >  2:13207/1‑151 (MQ=255)                                                                                                                                         ccGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCATAACGGCGTACGTTGGATTAACGATTCGAAAGCGACCAACGTCGGCAGTACGGAAGCGGCGCTGAATGGCCTGCACg  <  2:885005/151‑1 (MQ=255)                                                                                                                                              |                                                                                                                                                  GAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTCCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGTGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCATAACGGCGTACGTTGGATTAACGATTCGAAAGCGACCAACGTCGGCAGTACGGAAGCGGCGCTGAATGGCCTGCACG  >  NC_000913/97806‑98092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑