Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	592,635	A→G	F182L (TTC→CTC)	nfrB ←	bacteriophage N4 receptor, inner membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	592,635	0	A	G	100.0%	196.3 / NA	53	F182L (TTC→CTC)	nfrB	bacteriophage N4 receptor, inner membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/27);  total (26/27)

GAGGGCTTCACGCACCGGAACATCTTTGCCATGCAGCTCTGAAAACTCATCAATGTAAGTCATGCTGGTGAAGTGCGTCCATTCGCGTTCGAACGGATACACCGGGATCTGAATCAGATCTTTACGCTCGACCAGATAGTTGAACAGACGCAATTCCATCGGTGAAATCACATCTTCGGCGTCATGCAGAATAAAACCAGCAAAAGCGAAATTGGCGCTACGCTCAAATTGGGTGATGGCGTCCAGCACGTTGTTCAGACAGTCGGCTTTGCTGGTGGGGCCAGGAC  >  NC_000913/592492‑592778                                                                                                                                                |                                                                                                                                                gAGGGCTTCACGCACCGGAACATCTTTGCCATGCAGCTCTGAAAACTCATCAATGTAAGTCATGCTGGTGAAGTGCGTCCATTCGCGTTCGAACGGATACACCGGGATCTGAATCAGATCTTTACGCTCGACCAGATAGTTGAGCAGACGc                                                                                                                                          >  1:695203/1‑151 (MQ=255)   gggCTTCACGCACCGGAACATCTTTGCCATGCAGCTCTGAAAACTCATCAATGTAAGTCATGCTGGTGAAGTGCGTCCATTCGCGTTCGAACGGATACACAGGGATCTGAATCAGATCTTTACGCTCGACCAGATAGTTGAGCAGACGCa                                                                                                                                         <  2:585963/150‑1 (MQ=255)           cGCACCGGAACATCTTTGCCATGCAGCTCTGAAAACTCATCAATGTAAGTCATGCTGGTGAAGTGCGTCCATTCGCGTTCGAACGGATACACCGGGATCTGAATCAGATCTTTACGCTCGACCAGATAGTTGAGCAGACGCAATTCCATCg                                                                                                                                <  2:695154/151‑1 (MQ=255)           cGCACCGGAACATCTTTGCCATGCAGCTCTGAAAACTCATCAATGTAAGTCATGCTGGTGAAGTGCGTCCATTCGCGTTCGAACGGATACACCGGGATCTGAATCAGATCTTTACGCTCGACCAGATAGTTGAGCAGACGCAATTCCATCg                                                                                                                                <  1:346610/151‑1 (MQ=255)             cACCGGACCATCTTTGCCATGCAGCTCTGAAAACTCATCAATGTAAGTCATGCTGGTGAAGTGCGTCCATTCGCGTTCGAACGGATACACCGGGATCTGAATCAGAGCTTTACGCTCGTCCAGATAGTTGAGCAGACGCAATTCCATCGGt                                                                                                                              <  2:654934/151‑1 (MQ=255)              aCCGGAACATCTTTGCCATGCAGCTCTGAAAACTCATCAATGTAAGTCATGCTGGTGAAGTGCGTCCATTCGCGTTCGAACGGATACACCGGGATCTGAATCAGATCTTTACGCTCGACCAGATAGTTGAGCAGACGCAATTCCATCGGt                                                                                                                              >  1:575929/1‑150 (MQ=255)              aCCGGAACATCTTTGCCATGCAGCTCTGAAAACTCATCAATGTAAGTCATGCTGGTGAAGTGCGTCCATTCGCGTTCGAACGGATACACCGGGATCTGAATCAGATCTTTACGCTCGACCAGATAGTTGAGCAGACGCAATTCCATCGGTg                                                                                                                             <  2:645053/151‑1 (MQ=255)               ccGGAACATCTTTGCCATGCAGCTCTGAAAACTCATCAATGTAAGTCATGCTGGTGAAGTGCGTCCATTCGCGTTCGAACGGATACACCGGGATCTGAATCAGATCTTTACGCTCGACCAGATAGTTGAGCAGACGCAATTCCATCGGTGa                                                                                                                            <  2:231362/151‑1 (MQ=255)                cGGAACATCTTTGCCATGCAGCTCTGAAAACTCATCAATGTAAGTCATGCTGGTGAAGTGCGTCCATTCGCGTTCGAACGGATACACCGGGATCTGAATCAGATCTTTACGCTCGACCAGATAGTTGAGCAGACGCAATTCCATCGGTGaa                                                                                                                           <  2:586426/151‑1 (MQ=255)                            gCCATGCAGCTCTGAAAACTCATCAATGTAAGTCATGCTGGTGAAGTGCGTCCATTCGCGTTCGAACGGATACACCGGGATCTGAATCAGATCTTTACGCTCGACCAGATAGTTGAGCAGACGCAATTCCATCGGTGAAATCACATCTTCg                                                                                                               <  2:803863/151‑1 (MQ=255)                                  cAGCTCTGAAAACTCATCAATGTAAGTCATGCTGGTGAAGTGCGTCCATTCGCGTTCGAACGGATACACCGGGATCTGAATCAGATCTTTACGCTCGACCAGATAGTTGAGCAGACGCAATTCCATCGGTGAAATCACATCTTCGGCGTc                                                                                                          >  2:668769/1‑150 (MQ=255)                                   aGCTCTGAAAACTCATCAATGTAAGTCATGCTGGTGAAGTGCGTCCATTCGCGTTCGAACGGATACACCGGGATCTGAATCAGATCTTTACGCTCGACCAGATAGTTGAGCAGACGCAATTCCATCGGTGAAATCACATCTTCGGCGTCAt                                                                                                        <  1:503682/151‑1 (MQ=255)                                      tctGAAAACTCATCAATGTAAGTCATGCTGGTGAAGTGCGTCCATTCGCGTTCGAACGGATACACCGGGATCTGAATCAGATCTTTACGCTCGACCAGATAGTTGAGCAGACGCAATTCCATCGGTGAAATCACATCTTCGGCGTCATGc                                                                                                      >  2:24623/1‑150 (MQ=255)                                         gAAAACTCATCAATGTAAGTCATGCTGGTGAAGTGCGTCCATTCGCGTTCGAACGGATACACCGGGATCTGAATCAGATCTTTACGCTCGACCAGATAGTTGAGCAGACGCAATTCCATCGGTGAAATCACATCTTCGGCGTCATGCa                                                                                                     <  2:443866/148‑1 (MQ=255)                                         gAAAACTCATCAATGTAAGTCATGCTGGTGAAGTGCGTCCATTCGCGTTCGAACGGATACACCGGGATCTGAATCAGATCTTTACGCTCGACCAGATAGTTGAGCAGACGCAATTCCATCGGTGAAATCACATCTTCGGCGTCATGCAg                                                                                                    <  2:455526/149‑1 (MQ=255)                                         gAAAACTCATCAATGTAAGTCATGCTGGTGAAGTGCGTCCATTCGCGTTCGAACGGATACACCGGGATCTGAATCAGATCTTTACGCTCGACCAGATAGTTGAGCAGACGCAATTCCATCGGTGAAATCACATCTTCGGCGTCATGCAGaa                                                                                                  <  2:162447/151‑1 (MQ=255)                                         gAAAACTCATCAATGTAAGTCATGCTGGTGAAGTGCGTCCATTCGCGTTCGAACGGATACACCGGGATCTGAATCAGATCTTTACGCTCGACCAGATAGTTGAGCAGACGCAATTCCATCGGTGAAATCACATCTTCGGCGTCATGCAGa                                                                                                   >  2:416873/1‑150 (MQ=255)                                            aaCTCATCAATGTAAGTCATGCTGGTGAAGTGCGTCCATTCGCGTTCGAACGGATACACCGGGATCTGAATCAGATCTTTACGCTCGACCAGATAGTTGAGCAGACGCAATTCCATCGGTGAAATCACATCTTCGGCGTCATGCAGaa                                                                                                  <  1:903006/148‑1 (MQ=255)                                            aaCTCATCAATGTAAGTCATGCTGGTGAAGTGCGTCCATTCGCGTTCGAACGGATACACCGGGATCTGAATCAGATCTTTACGCTCGACCAGATAGTTGAGCAGACGCAATTCCATCGGTGAAATCACATCTTCGGCGTCATGCAGaa                                                                                                  >  2:902933/1‑148 (MQ=255)                                            aaCTCATCAATGTAAGTCATGCTGGTGAAGTGCGTCCATTCGCGTTCGAACGGATACACCGGGATCTGAATCAGATCTTTACGCTCGACCAGATAGTTGAGCAGACGCAATTCCATCGGTGAAATCACATCTTCGGCGTCATGCAGAATa                                                                                                >  2:857823/1‑150 (MQ=255)                                               tcatcaATGTAAGTCATGCTGGTGAAGTGCGTCCATTCGCGTTCGAACGGATACACCGGGATCTGAATCAGATCTTTACGCTCGACCAGATAGTTGAGCAGACGCAATTCCATCGGTGAAATCACATCTTCGGCGTCATGCAGAATAAAAc                                                                                            >  2:444094/1‑151 (MQ=255)                                                catcaATGTAAGTCATGCTGGTGAAGTGCGTCCATTCGCGTTCGAACGGATACACCGGGATCTGAATCAGATCTTTACGCTCGACCAGATAGTTGAGCAGACGCAATTCCATCGGTGAAATCACATCTTCGGCGTCATGCAGAATAAAAcc                                                                                           <  1:385519/151‑1 (MQ=255)                                                catcaATGTAAGTCATGCTGGTGAAGTGCGTCCATTCGCGTTCGAACGGATACACCGGGATCTGAATCAGATCTTTACGCTCGACCAGATAGTTGAGCAGACGCAATTCCATCGGTGAAATCACATCTTCGGCGTCATGCAGAATAAAAcc                                                                                           >  2:787744/1‑151 (MQ=255)                                                catcaATGTAAGTCATGCTGGTGAAGTGCGTCCATTCGCGTTCGAACGGATACACCGGGATCTGAATCAGATCTTTACGCTCGACCAGATAGTTGAGCAGACGCAATTCCATCGGTGAAATCACATCTTCGGCGTCATGCAGAATAAAAc                                                                                            <  1:13703/150‑1 (MQ=255)                                                   caATGTAAGTCATGCTGGTGAAGTGCGTCCATTCGCGTTCGAACGGATACACCGGGATCTGAATCAGATCTTTACGCTCGACCAGATAGTTGAGCAGACGCAATTCCATCGGTGAAATCACATCTTCGGCGTCATGCAGAATAAAACCAg                                                                                         >  1:496795/1‑150 (MQ=255)                                                   caATGTAAGTCATGCTGGTGAAGTGCGTCCATTCGCGTTCGAACGGATACACCGGGATCTGAATCAGATCTTTACGCTCGACCAGATAGTTGAGCAGACGCAATTCCATCGGTGAAATCACATCTTCGGCGTCATGCAGAATAAAACCAg                                                                                         <  2:898726/150‑1 (MQ=255)                                                          aGTCATGCTGGTGAAGTGCGTCCATTCGCGTTCGAACGGATACACCGGGATCTGAATCAGATCTTTACGCTCGACCAGATAGTTGAGCAGACGCAATTCCATCGGTGAAATCACATCTTCGGCGTCATGCAGAATAAAACCAGCAAAAGCg                                                                                 <  1:790701/151‑1 (MQ=255)                                                              aTGCTGGTGAAGTGCGTCCATTCGCGTTCGAACGGATACACCGGGATCTGAATCAGATCTTTACGCTCGACCAGATAGTTGAGCAGACGCAATTCCATCGGTGAAATCACATCTTCGGCGTCATGCAGAATAAAACCAGCAAAAGCGAAAt                                                                             >  2:384453/1‑151 (MQ=255)                                                                       aaGTGCGTCCATTCGCGTTCGAACGGATACACCGGGATCTGAATCAGATCTTTACGCTCGACCAGATAGTTGAGCAGACGCAATTCCATCGGTGAAATCACATCTTCGGCGTCATGCAGAATAAAACCAGCAAAAGCGAAATTGGCGCTAc                                                                    >  2:528285/1‑151 (MQ=255)                                                                         gTGCGTCCATTCGCGTTCGAACGGATACACCGGGATCTGAATCAGATCTTTACGCTCGACCAGATAGTTGAGCAGACGCAATTCCATCGGTGAAATCACATCTTCGGCGTCATGCAGAATAAAACCAGCAAAAGCGAAATTGGCGCTACGc                                                                  >  2:371075/1‑151 (MQ=255)                                                                         gTGCGTCCATTCGCGTTCGAACGGATACACCGGGATCTGAATCAGATCTTTACGCTCGACCAGATAGTTGAGCAGACGCAATTCCATCGGTGAAATCACATCTTCGGCGTCATGCAGAATAAAACCAGCAAAAGCGAAATTGGCGCTACGc                                                                  <  2:249158/151‑1 (MQ=255)                                                                           gCGTCCATTCGCGTTCGAACGGATACACCGGGATCTGAATCAGATCTTTACGCTCGACCAGATAGTTGAGCAGACGCAATTCCATCGGTGAAATCACATCTTCGGCGTCATGCAGAATAAAACCAGCAAAAGCGAAATTGGCGCTACGCt                                                                 <  1:180210/150‑1 (MQ=255)                                                                             gTCCATTCGCGTTCGAACGGATACACCGGGATCTGAATCAGATCTTTACGCTCGACCAGATAGTTGAGCAGACGCAATTCCATCGGTGAAATCACATCTTCGGCGTCATGCAGAATAAAACCAGCAAAAGCGAAATTGGCGCTACGCTCa                                                               >  2:844082/1‑150 (MQ=255)                                                                               ccATTCGCGTTCGAACGGATACACCGGGATCTGAATCAGATCTTTACGCTCGACCAGATAGTTGAGCAGACGCAATTCCATCGGTGAAATCACATCTTCGGCGTCATGCAGAATAAAACCAGCAAAAGCGAAATTGGCGCTACGCTCAAAt                                                            <  2:770116/151‑1 (MQ=255)                                                                                    cgcgTTCGAACGGATACACCGGGATCTGAATCAGATCTTTACGCTCGACCAGATAGTTGAGCAGACGCAATTCCATCGGTGAAATCACATCTTCGGCGTCATGCAGAATAAAACCAGCAAAAGCGAAATTGGCGCTACGCTCAAATTGGGt                                                       <  2:158143/151‑1 (MQ=255)                                                                                         tCGAACGGATACACCGGGATCTGAATCAGATCTTTACGCTCGACCAGATAGTTGAGCAGACGCAATTCCATCGGTGAAATCACATCTTCGGCGTCATGCAGAATAAAACCAGCAAAAGCGAAATTGGCGCTACGCTCAAATTGGGTGATg                                                   <  1:221717/150‑1 (MQ=255)                                                                                          cGAACGGATACACCGGGATCTGAATCAGATCTTTACGCTCGACCAGATAGTTGAGCAGACGCAATTCCATCGGTGAAATCACATCTTCGGCGTCATGCAGAATAAAACCAGCAAAAGCGAAATTGGCGCTACGCTCAAATTGGGTGATGGc                                                 >  2:504926/1‑151 (MQ=255)                                                                                            aaCGGATACACCGGGATCTGAATCAGATCTTTACGCTCGACCAGATAGTTGAGCAGACGCAATTCCATCGGTGAAATCACATCTTCGGCGTCATGCAGAATAAAACCAGCAAAAGCGAAATTGGCGCTACGCTCAAATTGGGTGATGGCGt                                               >  2:457812/1‑151 (MQ=255)                                                                                             aCGGATACACCGGGTTCTGAATCAGTTCTTTACGCTCGACCAGATAGTTGAGCAGACGCAATTCCATCGGTGAATTCACATCTTCGGCGTCATGCAGAATAAAACCAGCAAAAGCGAAATTGGCGCTACGCTCAAATTGGGTGATGGCGTc                                              >  2:882041/1‑151 (MQ=255)                                                                                             aCGGATACACCGGGATCTGAATCAGATCTTTACGCTCGACCAGATAGTTGAGCAGACGCAATTCCATCGGTGAAATCACATCTTCGGCGTCATGCAGAATAAAACCAGCAAAAGCGAAATTGGCGCTACGCTCAAATTGGGTGATGGCGTc                                              <  2:445314/151‑1 (MQ=255)                                                                                                 aTACACCGGGATCTGAATCAGATCTTTACGCTCGACCAGATAGTTGAGCAGACGCAATTCCATCGGTGAAATCACATCTTCGGCGTCATGCAGAATAAAACCAGCAAAAGCGAAATTGGCGCTACGCTCAAATTGGGTGATGGCGTCCAGc                                          >  1:132150/1‑151 (MQ=255)                                                                                                  tACACCGGGATCTGAATCAGATCTTTACGCTCGACCAGATAGTTGAGCAGACGCAATTCCATCGGTGAAATCACATCTTCGGCGTCATGCAGAATAAAACCAGCAAAAGCGAAATTGGCGCTACGCTCAAATTGGGTGATGGCGTCCAGc                                          >  1:136901/1‑150 (MQ=255)                                                                                                       cGGGATCTGAATCAGATCTTTACGCTCGACCAGATAGTTGAGCAGACGCAATTCCATCGGTGAAATCACATCTTCGGCGTCATGCAGAATAAAACCAGCAAAAGCGAAATTGGCGCTACGCTCAAATTGGGTGATGGCGTCCAGCACgttg                                    >  1:466007/1‑151 (MQ=255)                                                                                                          gATCTGAATCAGATCTTTACGCTCGACCAGATAGTTGAGCAGACGCAATTCCATCGGTGAAATCACATCTTCGGCGTCATGCAGAATAAAACCAGCAAAAGCGAAATTGGCGCTACGCTCAAATTGGGTGATGGCGTCCAGCACGTTGTTc                                 >  1:731420/1‑151 (MQ=255)                                                                                                              tGAATCAGATCTTTACGCTCGACCAGATAGTTGAGCAGACGCAATTCCATCGGTGAAATCACATCTTCGGCGTCATGCAGAATAAAACCAGCAAAAGCGAAATTGGCGCTACGCTCAAATTGGGTGATGGCGTCCAGCACGTTGTTCAGAc                             >  1:275094/1‑151 (MQ=255)                                                                                                              tGAATCAGATCTTTACGCTCGACCAGATAGTTGAGCAGACGCAATTCCATCGGTGAAATCACATCTTCGGCGTCATGCAGAATAAAACCAGCAAAAGCGAAATTGGCGCTACGCTCAAATTGGGTGATGGCGTCCAGCACGTTGTTCAGAc                             >  2:89772/1‑151 (MQ=255)                                                                                                                  tCAGATCTTTACGCTCGACCAGATAGTTGAGCAGACGCAATTCCATCGGTGAAATCACATCTTCGGCGTCATGCAGAATAAAACCAGCAAAAGCGAAATTGGCGCTACGCTCAAATTGGGTGATGGCGTCCAGCACGTTGTTCAGACAGTc                         >  2:69309/1‑151 (MQ=255)                                                                                                                    aGATCTTTACGCTCGACCAGATAGTTGAGCAGACGCAATTCCATCGGTGAAATCACATCTTCGGCGTCATGCAGAATAAAACCAGCAAAAGCGAAATTGGCGCTACGCTCAAATTGGGTGATGGCGTCCAGCACGTTGTTCAGACAGTCgg                       <  1:221777/151‑1 (MQ=255)                                                                                                                          ttACGCTCGACCAGATAGTTGAGCAGACGCAATTCCATCGGTGAAATCACATCTTCGGCGTCATGCAGAATAAAACCAGCAAAAGCGAAATTGGCGCTACGCTCAAATTGGGTGATGGCGTCCAGCACGTTGTTCAGACAGTCGGc                      <  1:661265/146‑1 (MQ=255)                                                                                                                          ttACGCTCGACCAGATAGTTGAGCAGACGCAATTCCATCGGTGAAATCACATCTTCGGCGTCATGCAGAATAAAACCAGCAAAAGCGAAATTGGCGCTACGCTCAAATTGGGTGATGGCGTCCAGCACGTTGTTCAGACAGTCGGCttt                   <  2:765890/149‑1 (MQ=255)                                                                                                                               cTCGACCAGATAGTTGAGCAGACGCAATTCCATCGGTGAAATCACATCTTCGGCGTCATGCAGAATAAAACCAGCAAAAGCGAAATTGGCGCTACGCTCAAATTGGGTGATGGCGTCCAGCACGTTGTTCAGACAGTCGGCTTTGCtggtg            >  1:238196/1‑151 (MQ=255)                                                                                                                                        aTAGTTGAGCAGACGCAATTCCATCGGGGAAATCACATCTTCGGCGTCATGCAGAATAAAACCAGCAAAAGCGAAATTGGCGCTACGCTCAAATTGGGTGATCGCGTCCAGCACGTTGTTCAGACAGTCGGCTTTGCTGGTGGGGCCAgg    >  1:343884/1‑150 (MQ=255)                                                                                                                                          aGTTGAGCAGACGCAATTCCATCGGTGAAATCACATCTTCGGCGTCATGCAGAATAAAACCAGCAAAAGCGAAATTGGCGCTACGCTCAAATTGGGTGATGGCGTCCAGCACGTTGTTCAGACAGTCGGCTTTGCTGGTGGGGCCAGGAc  <  2:880798/150‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                GAGGGCTTCACGCACCGGAACATCTTTGCCATGCAGCTCTGAAAACTCATCAATGTAAGTCATGCTGGTGAAGTGCGTCCATTCGCGTTCGAACGGATACACCGGGATCTGAATCAGATCTTTACGCTCGACCAGATAGTTGAACAGACGCAATTCCATCGGTGAAATCACATCTTCGGCGTCATGCAGAATAAAACCAGCAAAAGCGAAATTGGCGCTACGCTCAAATTGGGTGATGGCGTCCAGCACGTTGTTCAGACAGTCGGCTTTGCTGGTGGGGCCAGGAC  >  NC_000913/592492‑592778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑