Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	684,342	G→A	A24V (GCC→GTC)	rihA ←	ribonucleoside hydrolase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	684,342	0	G	A	100.0%	200.9 / NA	53	A24V (GCC→GTC)	rihA	ribonucleoside hydrolase 1
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/17);  total (36/17)

ACGCATTAACGGTTTTACCGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACAGATACGAGGGGCGAAACG  >  NC_000913/684194‑684484                                                                                                                                                     |                                                                                                                                               aCGCATTAACGGTTTTACCGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAgacg                                                                                                                                              >  1:573530/1‑151 (MQ=255)       tAACGGTTTTACCGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAgacgagggc                                                                                                                                         <  1:745583/150‑1 (MQ=255)         aCGGTTTTACCGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAgacgagggcg                                                                                                                                        >  1:813199/1‑149 (MQ=255)          cGGTTTTACCGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCgaga                                                                                                                                     <  2:858089/151‑1 (MQ=255)           ggTTTTACCGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCgaga                                                                                                                                     >  1:710626/1‑150 (MQ=255)                 aCCGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTAtt                                                                                                                               <  1:488451/150‑1 (MQ=255)                 aCCGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTg                                                                                                                              >  1:37945/1‑151 (MQ=255)                    gcgcCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCg                                                                                                                            >  1:473641/1‑150 (MQ=255)                                  cGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGa                                                                                                             <  1:81885/151‑1 (MQ=255)                                    gAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGAcc                                                                                                           <  2:346064/151‑1 (MQ=255)                                       tatCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTgg                                                                                                        >  2:476028/1‑151 (MQ=255)                                       tatCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTgg                                                                                                        >  2:577737/1‑151 (MQ=255)                                        atCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTggg                                                                                                       >  2:701339/1‑151 (MQ=255)                                              gCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCa                                                                                                  <  1:847586/150‑1 (MQ=255)                                                gATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATc                                                                                               >  1:612241/1‑151 (MQ=255)                                                        aaGGTCAGCATACGCAGAACATTGCGTAAGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGa                                                                                        >  1:880697/1‑150 (MQ=255)                                                           gTCAGCATACGCAGAACATTGCGTAAGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTg                                                                                    <  1:517252/151‑1 (MQ=255)                                                              aGCATACGCAGAACATTGCGTAAGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGc                                                                                  >  2:354066/1‑150 (MQ=255)                                                              aGCATACGCAGAACATTGCGTAAGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCa                                                                                 <  2:846281/151‑1 (MQ=255)                                                                 aTACGCAGAACATTGCGTAAGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTg                                                                              <  1:827438/151‑1 (MQ=255)                                                                  tACGCAGAACATTGCGTAAGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGc                                                                             >  1:349993/1‑151 (MQ=255)                                                                  tACGCAGAACATTGCGTAAGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGc                                                                             >  2:844863/1‑151 (MQ=255)                                                                       aGAACATTGCGTAAGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCAttg                                                                        >  2:163571/1‑151 (MQ=255)                                                                         aaCATTGCGTAAGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCAttgtt                                                                      >  2:62413/1‑151 (MQ=255)                                                                           cATTGCGTAAGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGc                                                                    <  1:696413/151‑1 (MQ=255)                                                                           cATTGCGTAAGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGc                                                                    >  1:787017/1‑151 (MQ=255)                                                                             ttGCGTAAGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTc                                                                  >  2:904901/1‑151 (MQ=255)                                                                                  tAAGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCttgt                                                             >  2:806946/1‑151 (MQ=255)                                                                                   aaGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCttgtt                                                            >  1:779955/1‑151 (MQ=255)                                                                                     ggTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTtgttgt                                                          >  1:125011/1‑151 (MQ=255)                                                                                      gTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTtgtg                                                         >  2:356504/1‑151 (MQ=255)                                                                                        tttttCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTtgtgt                                                        <  1:886914/150‑1 (MQ=255)                                                                                           ttCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGctt                                                    >  2:435781/1‑151 (MQ=255)                                                                                           ttCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGctt                                                    >  1:338982/1‑151 (MQ=255)                                                                                            tCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGcttc                                                   >  2:530169/1‑151 (MQ=255)                                                                                            tCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGcttc                                                   >  2:152465/1‑151 (MQ=255)                                                                                             cTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGCGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGACGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGcttct                                                  >  1:340324/1‑151 (MQ=255)                                                                                             cTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGcttct                                                  >  1:375977/1‑151 (MQ=255)                                                                                                 tgtCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGc                                              >  1:575489/1‑151 (MQ=255)                                                                                                     tGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGAc                                           >  2:362753/1‑150 (MQ=255)                                                                                                       gTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACa                                          >  2:291691/1‑149 (MQ=255)                                                                                                          tCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAggg                                      <  2:805916/150‑1 (MQ=255)                                                                                                           ccGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTTCTTGTTGTGTGCTTCTTTGCGACAAGGGTa                                    <  2:679882/151‑1 (MQ=255)                                                                                                           ccGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTa                                    <  1:91307/151‑1 (MQ=255)                                                                                                              gcggAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACg                                 >  2:181967/1‑151 (MQ=255)                                                                                                                    gACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGa                           <  1:270841/151‑1 (MQ=255)                                                                                                                      cGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATg                         >  1:337300/1‑151 (MQ=255)                                                                                                                          aTTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAAc                     <  1:693497/151‑1 (MQ=255)                                                                                                                            tGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAAc                     >  1:606117/1‑149 (MQ=255)                                                                                                                             gCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAAc                     >  2:182848/1‑148 (MQ=255)                                                                                                                                   aCATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACAGATACGAg            <  1:237742/151‑1 (MQ=255)                                                                                                                                     aTCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACAGATACGAggg          <  2:215493/151‑1 (MQ=255)                                                                                                                                     aTCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACAGATACGAggg          >  1:215452/1‑151 (MQ=255)                                                                                                                                         aGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGAAACGCCAGGATGTAACAGATACGAGGGGCg       >  1:420016/1‑150 (MQ=255)                                                                                                                                            tctGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACAGATACGAGGGGCGAAAc   >  2:860135/1‑151 (MQ=255)                                                                                                                                             ctGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACAGATACGAGGGGCGAAACg  >  2:685332/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                               ACGCATTAACGGTTTTACCGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACAGATACGAGGGGCGAAACG  >  NC_000913/684194‑684484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑