Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	146,238	A→G	K386K (AAA→AAG)	yadE →	putative polysaccharide deacetylase lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	146,238	0	A	G	100.0%	196.4 / NA	54	K386K (AAA→AAG)	yadE	putative polysaccharide deacetylase lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/28);  total (26/28)

AATTTAATCCGCATGTCTGGTATCTTTCGTATCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGT  >  NC_000913/146090‑146384                                                                                                                                                     |                                                                                                                                                   aaTTTAATCCGCATGTCTGGTATCTTTCGTATCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCg                                                                                                                                                  <  2:801224/151‑1 (MQ=255)     tAATCCGCATGTCTGGTATCTTTCGTATCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACtt                                                                                                                                              >  1:576949/1‑151 (MQ=255)      aaTCCGCATGTCTGGTATCTTTCGTATCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACttt                                                                                                                                             <  1:391073/151‑1 (MQ=255)         ccGCATGTCTGGTATCTTTCGTATCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTtata                                                                                                                                          <  1:371856/151‑1 (MQ=255)         ccGCATGTCTGGTATCTTTCGTATCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTtat                                                                                                                                           >  1:2215/1‑150 (MQ=255)           gCATGTCTGGTATCTTTCGTATCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATc                                                                                                                                        <  2:259265/151‑1 (MQ=255)               gTCTGGTATCTTTCGTATCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTa                                                                                                                                     >  1:884844/1‑150 (MQ=255)                  tGGTATCTTTCGTATCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGa                                                                                                                                  >  1:483080/1‑150 (MQ=255)                      aTCTTTCGTATCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACgg                                                                                                                              <  1:219024/150‑1 (MQ=255)                          ttCGTATCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGa                                                                                                                             <  1:7233/147‑1 (MQ=255)                          ttCGTATCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGAtt                                                                                                                           <  2:373264/149‑1 (MQ=255)                          ttCGTATCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCg                                                                                                                         >  1:407215/1‑151 (MQ=255)                          ttCGTATCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACT‑AAGCGACTTTATATCTTAAGAACGGATTc                                                                                                                          <  1:409724/149‑1 (MQ=255)                             gTATCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTg                                                                                                                      >  2:404042/1‑151 (MQ=255)                             gTATCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTg                                                                                                                      <  1:462425/151‑1 (MQ=255)                                 ccGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAAACATGAAAGGCAAAGTAACACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTgg                                                                                                                     >  2:466325/1‑148 (MQ=255)                                      tGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGAt                                                                                                              <  1:297715/150‑1 (MQ=255)                                       ggCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATg                                                                                                             <  1:881342/150‑1 (MQ=255)                                         cGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTcg                                                                                                          >  1:377038/1‑151 (MQ=255)                                          ggATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTcgc                                                                                                         >  1:870248/1‑151 (MQ=255)                                           gATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTcgcg                                                                                                        <  1:142217/151‑1 (MQ=255)                                               tAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCt                                                                                                     <  1:430244/150‑1 (MQ=255)                                                aaTGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTgg                                                                                                   >  1:899510/1‑151 (MQ=255)                                                aaTGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTgg                                                                                                   >  1:278688/1‑151 (MQ=255)                                                   gACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCGCCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGa                                                                                                <  2:342953/151‑1 (MQ=255)                                                          ccGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAAcc                                                                                          >  1:594249/1‑150 (MQ=255)                                                            gTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGc                                                                                       >  2:586246/1‑151 (MQ=255)                                                                aGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCg                                                                                     <  1:552404/149‑1 (MQ=255)                                                                      caAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATa                                                                             <  1:664730/151‑1 (MQ=255)                                                                             tGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATc                                                                       >  1:377424/1‑150 (MQ=255)                                                                                 ggATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGc                                                                   >  1:78492/1‑150 (MQ=255)                                                                                     ttCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAAct                                                               <  2:439066/150‑2 (MQ=255)                                                                                     ttCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCt                                                              >  2:352039/1‑151 (MQ=255)                                                                                       cACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTg                                                             <  2:378378/150‑1 (MQ=255)                                                                                                   aCAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACGTGTACCGGAATCGCt                                                <  1:243304/151‑1 (MQ=255)                                                                                                      aCCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTc                                             >  1:42988/1‑151 (MQ=255)                                                                                                              aGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACg                                     >  1:615281/1‑151 (MQ=255)                                                                                                               ggCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGt                                    <  2:417474/151‑1 (MQ=255)                                                                                                                   aaGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGtttca                                <  1:647367/151‑1 (MQ=255)                                                                                                                   aaGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGtttc                                 >  1:801171/1‑150 (MQ=255)                                                                                                                     gTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGtttcatt                              >  1:152895/1‑151 (MQ=255)                                                                                                                                ggATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGcc                   <  1:31535/151‑1 (MQ=255)                                                                                                                                  aTAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCtt                 >  1:261891/1‑151 (MQ=255)                                                                                                                                    aaTCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTc                <  1:796360/150‑1 (MQ=255)                                                                                                                                    aaTCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTc                >  1:233578/1‑150 (MQ=255)                                                                                                                                       ccGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCaaa             >  1:35992/1‑150 (MQ=255)                                                                                                                                        cGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAAt           <  2:101120/151‑1 (MQ=255)                                                                                                                                         gttgttACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATa          <  1:233504/151‑1 (MQ=255)                                                                                                                                            gttACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAgg        <  2:661712/150‑1 (MQ=255)                                                                                                                                            gttACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGc       <  2:280851/151‑1 (MQ=255)                                                                                                                                             ttACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCg      >  2:148700/1‑151 (MQ=255)                                                                                                                                              tACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCg      >  2:305464/1‑150 (MQ=255)                                                                                                                                              tACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCg      <  1:46742/150‑1 (MQ=255)                                                                                                                                                 tAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGt  >  2:281611/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                   AATTTAATCCGCATGTCTGGTATCTTTCGTATCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGT  >  NC_000913/146090‑146384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑