Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	686,137	T→C	N178S (AAC→AGC)	gltJ ←	glutamate/aspartate ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	686,137	0	T	C	100.0%	200.5 / NA	55	N178S (AAC→AGC)	gltJ	glutamate/aspartate ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/21);  total (34/21)

CATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAA‑CGACTCCCAGGCATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGCGACTGAATCGCGGCGC  >  NC_000913/685989‑686278                                                                                                                                                      |                                                                                                                                              cATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAA‑CGACTCCCAGGCATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAAACCGATAGTCCAGGCGATGGCGGAGTTTTTCACCAGGCtc                                                                                                                                             >  1:566410/1‑151 (MQ=255)    aaaaaagGCGTTAATAAACACACCCCCCAG‑GTAAAGGGGGTAAACCGCCCCCCAGGCGTGGGCGGATAAACGGAGAAATTCCCCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGCtcat                                                                                                                                           <  2:744884/147‑1 (MQ=255)                      cacaTACGCCAGCGTAATGGCGGTAAA‑CGACTCCCAGGCATGGGCTGAGTAAACGAGCAATTTTCCCGCCTGCGCCCCCATATCCACCAGAACGATAGTCGAGGCGATGGCGGGGTTTTTTACCAGGCTCATCATCTCTGAGGGCATcgg                                                                                                                         >  2:318422/1‑150 (MQ=255)                              ccAGCGTAATGGCGGTAAA‑CGACTCCCAGGCATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGCTCATCATCTCTGAGGGCATCGGCggg                                                                                                                     >  1:667355/1‑146 (MQ=255)                                aGCGTAATGGCGGTAAA‑CGACTCCCAGGCAGGGGCCGGGAAATCGAGCAATTTACCCGCCTGCGCCGCCATAACCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGCTCATCATCTCTGAGGTCATCGGCggggggcaa                                                                                                               >  1:401378/1‑144 (MQ=255)                                   gTAAGGGCGTAAAC‑CGCCCCCCAGGGAGGGGAGGAAAAACCGAGCATTTCCCCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGCTCATCATCTCTGAGGTCATCGGCGGGACGATAAc                                                                                                             <  2:784035/149‑1 (MQ=255)                                    tAATGGCGGTAAA‑AGACTCCCAGGCATGGGCTGAGTAATTGAGCAATTTACCCGCCCGCGCCGCCACATCCACCAAACCGATAGTCGAGGCGATGGCGGGGTTTTTCACCCGGCGCATCCTATCTGAGGTCATCGGCgggggg                                                                                                                  >  2:129771/1‑140 (MQ=255)                                                   gACTCCCAGGCATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGCTCATCATCTCTGAGGTCATCGGCGGGACGCGAACACGATAAGCaattgg                                                                                              >  1:733228/1‑144 (MQ=255)                                                    aCTCCCAGGCATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGCTCATCATCTCTGAGGTCATCGGCGGGGAGGTAACGCGGTAAGCAATAGgc                                                                                             >  2:784147/1‑149 (MQ=255)                                                       cccAGGCATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGCTCATCATCTCTGAGGTCATCGGCGGGCCGATAACGCGGTAAGCATTAGgca                                                                                            >  1:22666/1‑147 (MQ=255)                                                          aGGCATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGCTCATCATCTCTGAGGTCATCGGCGGGACGATAAAGCGGTAAGCATTAGGGAGCAGTa                                                                                      >  1:121596/1‑150 (MQ=255)                                                               tGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGCTCATCATCTCTGAGGTCATCGGCGGGGCGGTAAAGCGATAAACATTAGGCAGCAGTAAGTAt                                                                                 >  2:298082/1‑150 (MQ=255)                                                                gggatgaaaaaTCAAGCATTTCCCCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGCTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCGGTAc                                                                                     <  2:380351/136‑1 (MQ=255)                                                                  gCTGATTAAGCGAAAATTTTCCCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGCTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGa                                                                              <  1:40588/150‑1 (MQ=255)                                                                  gCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGCTCATCATCTCTGAGGTCATCGGCGGGGCGGTAAAGCGATAAGCATTAGGCAGCAGGACGGATCGa                                                                              >  2:502459/1‑150 (MQ=255)                                                                     gAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGCTCATCATCTCTGAGGTCATCGGCGGGACGCAAACGCGATTATCGTTAGGCAGTAGATCGTaatga                                                                              >  1:583959/1‑143 (MQ=255)                                                                      aataaccgacaattttCCCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGCTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAg                                                                          <  1:262223/137‑1 (MQ=255)                                                                      aGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGCTCATCATCTCTGAGGTCATCGGCGGGGCGATAACGCGATAAGCATTTGGCCGCAGGACGTATCGa                                                                              >  2:81158/1‑146 (MQ=255)                                                                      aGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGCTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGGACGTATCGATaa                                                                           >  1:62109/1‑149 (MQ=255)                                                                        tAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGCTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAAcc                                                                        >  1:593624/1‑150 (MQ=255)                                                                         accgccactaTTCACCCGCCTGGGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGCTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTg                                                                      <  1:93117/142‑1 (MQ=255)                                                                             gAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGCTCATCATCTCTGAGGTCATCGGCGGGGCGGTAAAGCGGTAAGCATTAGGCAGCAGTACGTATCGATAAACCTGCgg                                                                   >  2:336664/1‑150 (MQ=255)                                                                                cAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGCTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGAAAAGCATTAGGCAGCAGTAAGTAACGATAAGCCTGCgg                                                                   >  1:406396/1‑147 (MQ=255)                                                                                 aaTTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGCTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAACCCGCGGGAGCg                                                              >  1:367471/1‑151 (MQ=255)                                                                                   tttCCCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGCTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTa                                                                 <  2:453786/146‑1 (MQ=255)                                                                                   tttCCCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGCTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAg                                                                <  2:19605/147‑1 (MQ=255)                                                                                   ttcccccGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGCTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAg                                                                <  2:196758/143‑1 (MQ=255)                                                                                     tACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGCTCATCATCTCTGAGGTCATCGGCGGGACGATAAAGCGATAAACAATAGGCAGCAGTACGTATCGATAAACCCGCGGGAGCg                                                              >  1:817747/1‑147 (MQ=255)                                                                                     caCCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGCTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTc                                                            <  2:94227/148‑1 (MQ=255)                                                                                      ccccGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGCTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAgg                                                         <  1:339998/150‑1 (MQ=255)                                                                                       cccGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGCTCATCATCTCTGAGGTCATCGGCGGGAAGATAACGCGATAAGCAATAGGCAGCAGAACGGATCGATAAACCTGCGGGAGCGTCAgg                                                         >  1:852059/1‑150 (MQ=255)                                                                                           ccTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGCTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCa                                                     <  2:817681/150‑1 (MQ=255)                                                                                            cTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGCTCATCATCTCTGAGGTCATCGGCGGGACGGTAACGCGATTAAAATTAGGGACCAGTAAGCATCCATAAACCAGCGGGAGGGGCAGGACCac                                                    >  2:854660/1‑149 (MQ=255)                                                                                            cTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGCTCATCATCTCTGAGGTCATCGGCGGGACGATAAAGCGATAAGCATTAGGGAGCAGTACGTATCGATAAACCTGCGGGAGAGGCAGGccc                                                      >  2:404919/1‑148 (MQ=255)                                                                                             tGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGCTCATCATCTCTGAGGTCATCGGCGGGACGGTAACGCGATAAGCATTAGGCAGCCGTACGTATCGATAAACCTGCGGGAGCGGCAGGCCCaac                                                   >  1:95160/1‑148 (MQ=255)                                                                                              gcgcCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGCTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATc                                                   <  1:283388/149‑1 (MQ=255)                                                                                               cgccgcCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGCTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGcc                                                <  1:442510/151‑1 (MQ=255)                                                                                                gccgccATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGCTCATCATCTCTGAGGTCATCGGCGGGACGATAAAGCGATCAGAATTTTGCAGCAGGACGTATCGATAAGCCCGCGGGAGGGCCAGGCCCATccccc                                               >  2:727994/1‑150 (MQ=255)                                                                                                 ccgccATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGCTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGcc                                                <  2:62087/149‑1 (MQ=255)                                                                                                  cgccATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGCTCATCATCTCTGAGGTCATCGGCGGGGCGGAAACGCGATAAACATTAGGCAGCAATAAGTATCGATAAACCTGCGGTAGCGGCAGGCCCATcccc                                                >  2:259541/1‑148 (MQ=255)                                                                                                     cATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGCTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAACCTGCGGGAGCGTCAGGCCCATCGCCAgcgc                                           >  1:80431/1‑150 (MQ=255)                                                                                                          ccaccaGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGCTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGGACGTATCGATAAACCTGCGGGAGCGTCAGGCCCATCGCCAGCGCGGCAtt                                     >  1:470378/1‑151 (MQ=255)                                                                                                            accaGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGCTCATCATCTCTGAGGTCATCGGCGGGACGATAAAGCGATAAGCATTAGGCAGCAGTACGTATCGATAAACCTGCGGGAGCGTCAGGCCCAACGCCCGCGCGGCCtttt                                   >  2:229811/1‑151 (MQ=255)                                                                                                             ccaGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGCTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCAttttt                                  <  1:318448/151‑1 (MQ=255)                                                                                                               aGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGCTCATCATCTCTGAGGTCATCGGCGGGACGATAAAGCGATAAGCATTAGGCAGCAGTACGTATCGATAAACCTGCGGTAGCGTCAGGCCCATCGCCACCGCGGCCttttttt                                >  1:752011/1‑151 (MQ=255)                                                                                                                     aTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGCTCATCATCGCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCTATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTc                          <  2:321818/151‑1 (MQ=255)                                                                                                                       aGTCGAGGCGATGGCGGAGTTTTTCACCAGGCTCATCATCTCTGAGGTCATCGGCGGGAGGAAAACGGGATAAGCATTAGGGCGCAGTACGAATCCGTAAGCCTGCGGTAGCGTGAGGCCCAACGCCAACGCGGCATTTTTTTTccc                            >  1:728485/1‑147 (MQ=255)                                                                                                                            aGGCGATGGCGGAGTTTTTCACCAGGCTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAg                   <  1:898399/151‑1 (MQ=255)                                                                                                                                gATGGCGGAGTTTTTCACCAGGCTCATCATCTCTGAGGTCATCGGCGGGGCGGTAAAGCGCTAAGCAATAGGCAGCGGGACGTATAGATAAACCTGCGGGAGCGTCAGGCCCATCCCCAGCGCGGGCTTTTTTTTCCCCCGCGGGAGCggc               >  2:13742/1‑149 (MQ=255)                                                                                                                                      ggAGTTTTTCACCAGGCTCATCATCTCTGAGGTCATCGGCGGGGCGATAACGCGATAAGCATTAAGTAGGAGTAAGCATTACTAAGTCCGCGGTAGCCTCATGCCCCACGCCCGCGCCGCAATTTTTTTCCCTCCCGGGCGCGACTTAATc         >  1:347467/1‑151 (MQ=255)                                                                                                                                      ggAGTTTTTCACCAGGCTCATCATCTCTGAGGTCATCGGCGGGGAGGGAACGAGGTAAGCATTTGGCAGCAGTTCGAATCGCTAAGCCCGCGGTAGGGTCCGGCCCAACCCCCCCGCGGCGTTTTTTTGCCCTCGCCGCGGCGGCTgtagc         >  2:768808/1‑147 (MQ=255)                                                                                                                                      ggAGTTTTTCACCAGGCTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGCGACTGAATc         <  1:13764/151‑1 (MQ=255)                                                                                                                                         gTTTTTCACCAGGCTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGCGACTGAATcgcg      <  2:80409/151‑1 (MQ=255)                                                                                                                                             ttCACCAGGCTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGCGACTGAATCgcggcg   <  2:307077/150‑1 (MQ=255)                                                                                                                                              tCACCAGGCTCATCATCTCTGAGGTCATCGGCGGGAAGGGAACGCGGTAAGCATTATGCAGCAGTACGTATCGGTAAGGCTTCGGTAGCGTCAGGCCCAACGCCCGCGCGGCATTTTTTTTTTCTCCCGGCGGCGACTGGATAGCGgcgc  >  1:665026/1‑150 (MQ=255)                                                                                                                                                      |                                                                                                                                              CATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAA‑CGACTCCCAGGCATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGCGACTGAATCGCGGCGC  >  NC_000913/685989‑686278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑