Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	699,448	A→G	intergenic (‑271/+126)	asnB ← / ← umpH	asparagine synthetase B/UMP phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	699,448	0	A	G	100.0%	202.6 / NA	56	intergenic (‑271/+126)	asnB/umpH	asparagine synthetase B/UMP phosphatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/30);  total (26/30)

ATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAG  >  NC_000913/699303‑699593                                                                                                                                                  |                                                                                                                                                  aTTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTGTCCg                                                                                                                                               <  2:256438/150‑1 (MQ=255)         aaaaaGGTCTGGTGTGATGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTGTCCGGCCTACaaa                                                                                                                                      <  1:442871/151‑1 (MQ=255)           aaaGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTGTCCGGCCTACAAAAg                                                                                                                                    <  2:582194/151‑1 (MQ=255)             aGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTGTCCGGCCTACAAAAGc                                                                                                                                   >  2:760974/1‑150 (MQ=255)               gTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTGTCCGGCCTACAAAAGCATg                                                                                                                                >  2:849840/1‑151 (MQ=255)                   ggTGTGAGGATATATTTATTGATGAATTGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTGTCCGGCCTACAAAAGCATGa                                                                                                                               <  1:826694/148‑1 (MQ=255)                   ggTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTGTCCGGCCTACAAAAGCATGaaa                                                                                                                             <  2:450012/150‑1 (MQ=255)                    gtgtGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTGTCCGGCCTACAAAAGCATGAAAAt                                                                                                                           >  2:853255/1‑151 (MQ=255)                      gtgAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTGTCCGGCCTACAAAAGCATGAAAAtt                                                                                                                          <  1:407855/150‑1 (MQ=255)                      gtgAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTGTCCGGCCTACAAAAGCATGAAAAtt                                                                                                                          <  2:849085/150‑1 (MQ=255)                         aGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTGTCCGGCCTACAAAAGCATGAAAATTCAat                                                                                                                      <  1:344199/151‑1 (MQ=255)                         aGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTGTCCGGCCTACAAAAGCATGAAAATTCAat                                                                                                                      >  2:879713/1‑151 (MQ=255)                         aGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTGTCCGGCCTACAAAAGCATGAAAATTCAat                                                                                                                      >  1:698588/1‑151 (MQ=255)                                atttattGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTGTCCGGCCTACAAAAGCATGAAAATTCAATATATTGc                                                                                                               >  1:718279/1‑151 (MQ=255)                                atttattGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTGTCCGGACTAGAAAAGCATGAAAATTCAATATAGTGc                                                                                                               <  2:562045/151‑1 (MQ=255)                                  ttattGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGGACGCCTTGTCCGGCCTACAAAAGCATGAAAATTCAATATATTGCa                                                                                                              <  2:816032/150‑1 (MQ=255)                                  ttattGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTGTCCGGCCTACAAAAGCATGAAAATTCAATATATTGCa                                                                                                              <  1:439962/150‑1 (MQ=255)                                  ttattGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTGTCCGGCCTACAAAAGCATGAAAATTCAATATATTGCa                                                                                                              <  2:165831/150‑1 (MQ=255)                                   tattGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTGTCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAgg                                                                                                            >  1:852413/1‑151 (MQ=255)                                    attGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGTGTGAACGCCTTGTCCGGCCTACAAAAGCATGAAAATTCAATATATTGAAgg                                                                                                            >  2:903397/1‑150 (MQ=255)                                           aaTCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTGTCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGt                                                                                                    >  1:345252/1‑151 (MQ=255)                                           aaTCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTGTCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGt                                                                                                    <  1:761024/151‑1 (MQ=255)                                              cGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTGTCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAg                                                                                                  <  2:440390/150‑1 (MQ=255)                                                       ttAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTGTCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATaa                                                                                         <  2:110329/150‑1 (MQ=255)                                                       ttAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTGTCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATa                                                                                          >  1:870174/1‑149 (MQ=255)                                                        tAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTGTCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGc                                                                                       >  1:100094/1‑151 (MQ=255)                                                          gCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTGTCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCg                                                                                      >  2:515519/1‑150 (MQ=255)                                                           cGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTGTCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCg                                                                                      >  1:627043/1‑149 (MQ=255)                                                           cGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTGTCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGt                                                                                     >  2:762909/1‑150 (MQ=255)                                                             ggTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTGTCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAgc                                                                                  <  1:226669/151‑1 (MQ=255)                                                             ggTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTGTCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAgc                                                                                  <  2:157989/151‑1 (MQ=255)                                                              gTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTGTCCGGCCTACAAAAGCATGAAAATTCAATATATCGCAGGAGCTGCGTAGGCCTGATAAGCGTAgcg                                                                                 <  2:500314/151‑1 (MQ=255)                                                                tttATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTGTCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAgcgc                                                                                >  1:502460/1‑150 (MQ=255)                                                                    ttGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTGTCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATc                                                                             >  1:840879/1‑149 (MQ=255)                                                                          gTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTGTCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGtt                                                                     >  2:294485/1‑151 (MQ=255)                                                                          gTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTGTCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGtt                                                                     >  1:578751/1‑151 (MQ=255)                                                                          gTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTGTCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGtt                                                                     >  1:563455/1‑151 (MQ=255)                                                                          gTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTGTCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGtt                                                                     >  1:380207/1‑151 (MQ=255)                                                                              atatTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTGTCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTgg                                                                  <  2:432663/150‑1 (MQ=255)                                                                                 ttttACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTGTCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGtt                                                              >  1:794781/1‑151 (MQ=255)                                                                                  tttACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTGTCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGtt                                                              <  1:265198/150‑1 (MQ=255)                                                                                         gggCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTGTCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGtcatca                                                      <  2:57977/151‑1 (MQ=255)                                                                                         gggCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTGTCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGtcatc                                                       >  2:88542/1‑150 (MQ=255)                                                                                               aaTTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTGTCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGcc                                                 >  2:526351/1‑150 (MQ=255)                                                                                                  tGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTGTCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAAc                                              <  2:248109/150‑1 (MQ=255)                                                                                                       aCAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTGTCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTc                                        <  2:600092/151‑1 (MQ=255)                                                                                                                     gTTCATGCCGGATGCGGCGTGAACGCCTTGTCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTg                          >  1:795654/1‑151 (MQ=255)                                                                                                                       tCATGCCGGATGCGGCGTGAACGCCTTGTCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGc                         <  2:607487/150‑1 (MQ=255)                                                                                                                         aTGCCGGATGCGGCGTGAACGCCTTGTCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTAt                      >  1:444348/1‑151 (MQ=255)                                                                                                                         aTGCCGGATGCGGCGTGAACGCCTTGTCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTAt                      >  1:896160/1‑151 (MQ=255)                                                                                                                           gCCGGATGCGGCGTGAACGCCTTGTCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTc                    <  2:696704/151‑1 (MQ=255)                                                                                                                                  gcggcgTGAACGCCTTGTCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATaa              <  2:442555/150‑1 (MQ=255)                                                                                                                                    ggcgTGAACGCCTTGTCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGt           <  1:242118/151‑1 (MQ=255)                                                                                                                                            cGCCTTGTCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCa   <  1:814639/151‑1 (MQ=255)                                                                                                                                            cGCCTTGTCCGGCCTACAAAAGCATGAAAATTCAATATAGTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGGGCCAACCACGTCGGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCa   <  2:865952/151‑1 (MQ=255)                                                                                                                                             gCCTTGTCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAg  <  2:798612/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                  ATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAG  >  NC_000913/699303‑699593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑