Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	150,128	A→G	S276P (TCT→CCT)	yadC ←	putative fimbrial‑like adhesin protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	150,128	0	A	G	100.0%	264.1 / NA	72	S276P (TCT→CCT)	yadC	putative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (43/29);  total (43/29)

TTCCAGTAAGCGTATTACCAAGCAGTTGTGTGTTTTGAGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGAGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGACATTATTCAGTGTGAAATTGACATAAATGTGGTTGCTGTGATCTACAACTTTTGT  >  NC_000913/149981‑150269                                                                                                                                                    |                                                                                                                                              ttCCAGTAAGCGTATTACCAAGCAGTTGTGTGTTTTGAGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCt                                                                                                                                            >  1:626292/1‑151 (MQ=255)  tCCAGTAAGCGTATTACCAAGCAGTTGTGTGTTTTGAGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCt                                                                                                                                            <  1:566344/150‑1 (MQ=255)  tCCAGTAAGCGTATTACCAAGCAGTTGTGTGTTTTGAGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCt                                                                                                                                            <  1:387350/150‑1 (MQ=255)     aGTAAGCGTATTACCAAGCAGTTGTGTGTTTTGAGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTatat                                                                                                                                        <  1:168938/151‑1 (MQ=255)         aGCGTATTACCAAGCAGTTGTGTGTTTTGAGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATtctc                                                                                                                                    <  2:771627/151‑1 (MQ=255)          gCGTATTACCAAGCAGTTGTGTGTTTTGAGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTcc                                                                                                                                   >  1:535391/1‑151 (MQ=255)             tATTACCAAGCAGTTGTGTGTTTTGAGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCAt                                                                                                                                >  1:696477/1‑151 (MQ=255)              aTTACCAAGCAGTTGTGTGTTTTGAGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCAtt                                                                                                                               >  2:187063/1‑151 (MQ=255)                      gCAGTTGTGTGTTTTGAGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGt                                                                                                                       >  2:668224/1‑151 (MQ=255)                      gCAGTTGTGTGTTTTGAGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGt                                                                                                                       <  1:212259/151‑1 (MQ=255)                        aGTTGTGTGTTTTGAGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTg                                                                                                                     >  1:663277/1‑151 (MQ=255)                          ttGTGTGTTTTGAGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGa                                                                                                                    >  1:121735/1‑150 (MQ=255)                                gtTTTGAGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCAtt                                                                                                              <  2:78313/150‑1 (MQ=255)                                gtTTTGAGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTg                                                                                                             >  2:510241/1‑151 (MQ=255)                                  tttGAGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGa                                                                                                            <  1:546152/150‑1 (MQ=255)                                    tGAGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACt                                                                                                          <  2:730099/150‑1 (MQ=255)                                    tGAGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTg                                                                                                         >  1:454072/1‑151 (MQ=255)                                     gAGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTg                                                                                                         >  2:80255/1‑150 (MQ=255)                                     gAGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTg                                                                                                         >  1:678021/1‑150 (MQ=255)                                      aGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGAt                                                                                                       <  1:528910/151‑1 (MQ=255)                                       gTACCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATg                                                                                                      <  1:237738/151‑1 (MQ=255)                                                 ttCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTa                                                                                              >  2:6082/1‑149 (MQ=255)                                                   ccAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGt                                                                                           >  1:607023/1‑150 (MQ=255)                                                   ccAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGACGGCCCGGTAAGt                                                                                           >  1:221492/1‑150 (MQ=255)                                                     aGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATg                                                                                        >  2:172194/1‑151 (MQ=255)                                                       tGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATgg                                                                                       >  1:767950/1‑150 (MQ=255)                                                               tttGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTaaaa                                                                               <  2:238046/150‑1 (MQ=255)                                                               tttGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAAc                                                                              >  1:166289/1‑151 (MQ=255)                                                                  gTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACAt                                                                            <  1:168338/150‑1 (MQ=255)                                                                   tttCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGt                                                                          >  1:371645/1‑151 (MQ=255)                                                                     tCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTg                                                                        >  2:110230/1‑151 (MQ=255)                                                                       aaTATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGt                                                                      <  1:24168/151‑1 (MQ=255)                                                                        atatTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGt                                                                      >  1:909026/1‑150 (MQ=255)                                                                        atatTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGt                                                                      >  1:717402/1‑150 (MQ=255)                                                                          atTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTaa                                                                    <  1:368473/150‑1 (MQ=255)                                                                            tACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGt                                                                 >  2:548083/1‑151 (MQ=255)                                                                              cGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACTATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGtt                                                                >  2:763155/1‑150 (MQ=255)                                                                              cGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTa                                                               <  1:841895/151‑1 (MQ=255)                                                                               gCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTa                                                               >  2:670645/1‑150 (MQ=255)                                                                                  cacGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAAttt                                                           <  1:225387/151‑1 (MQ=255)                                                                                    cGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTg                                                          >  1:360096/1‑150 (MQ=255)                                                                                    cGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTg                                                          >  1:459636/1‑150 (MQ=255)                                                                                    cGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTg                                                          >  2:155757/1‑150 (MQ=255)                                                                                       aTACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGACat                                                      <  2:225108/151‑1 (MQ=255)                                                                                         aCAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAGTTTGACatt                                                     >  2:908821/1‑150 (MQ=255)                                                                                         aCAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGACatt                                                     >  2:50422/1‑150 (MQ=255)                                                                                          cAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGACattat                                                   >  1:264070/1‑151 (MQ=255)                                                                                           aaTTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGACattatt                                                  <  1:413885/151‑1 (MQ=255)                                                                                             ttCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGACATTATTc                                                 >  1:731848/1‑150 (MQ=255)                                                                                              tCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGACATTATTCa                                                <  1:616181/150‑1 (MQ=255)                                                                                                 gAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGACATTATTCAgtg                                             >  2:132503/1‑150 (MQ=255)                                                                                                       agaTATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGACATTATTCAGTGTGAAAtt                                      <  1:854725/151‑1 (MQ=255)                                                                                                          tatCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGACATTATTCAGTGTGAAATTGAc                                   >  1:464809/1‑151 (MQ=255)                                                                                                               aaGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGACATTATTCAGTGTGAAATTGACATaaa                              <  2:518012/151‑1 (MQ=255)                                                                                                                aGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGACATTATTCAGTGTGAAATTGACATAAAt                             >  1:359317/1‑151 (MQ=255)                                                                                                                  ggAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGACATTATTCAGTGTGAAATTGACATAAAtgt                           >  2:363145/1‑151 (MQ=255)                                                                                                                   gAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGACATTATTCAGTGTGAAATTGACATAAAtgtg                          <  2:705155/151‑1 (MQ=255)                                                                                                                    aaCAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGACATTATTCAGTGTGAAATTGACATAAATGTgg                         <  2:622698/151‑1 (MQ=255)                                                                                                                    aaCAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGACATTATTCAGTGTGAAATTGACATAAATGTgg                         >  2:34428/1‑151 (MQ=255)                                                                                                                        ggTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGACATTATTCAGTGTGAAATTGACATAAATGTGGTTGc                     <  2:459589/151‑1 (MQ=255)                                                                                                                          tGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGACATTATTCAGTGTGAAATTGACATAAATGTGGTTGCtg                   >  2:223243/1‑151 (MQ=255)                                                                                                                           gAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGACATTATTCAGTGTGAAATTGACATAAATGTGGTTGCtgt                  >  2:832080/1‑151 (MQ=255)                                                                                                                             aGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGACATTATTCAGTGTGAAATTGACATAAATGTGGTTGCTGTGa                <  1:789651/151‑1 (MQ=255)                                                                                                                             aGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGACATTATTCAGTGTGAAATTGACATAAATGTGGTTGCTGTGa                <  1:326880/151‑1 (MQ=255)                                                                                                                             aGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGACATTATTCAGTGTGAAATTGACATAAATGTGGTTGCTGTGa                <  1:199288/151‑1 (MQ=255)                                                                                                                                   aTTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGACATTATTCAGTGTGAAATTGACATAAATGTGGTTGCTGTGATCTAc           >  2:848453/1‑150 (MQ=255)                                                                                                                                    tttttAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGGATGGAGGAAAAACATGTTGGTAAGGTTAATTTGACATTATTCAGTGTGAAATTGACATAAATGTGGTTGCTGTGATCTACa          >  1:130026/1‑150 (MQ=255)                                                                                                                                      tttAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGACATTATTCAGTGTGAAATTGACATAAATGTGGTTGCTGTGATCTACAACt       >  2:633536/1‑151 (MQ=255)                                                                                                                                      tttAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGACATTATTCAGTGTGAAATTGACATAAATGTGGTTGCTGTGATCTACAACt       >  2:493713/1‑151 (MQ=255)                                                                                                                                         aaTTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGACATTATTCAGTGTGAAATTGACATAAATGTGGTTGCTGTGATCTACAACttt     <  2:151856/150‑1 (MQ=255)                                                                                                                                          aTTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGACATTATTCAGTGTGAAATTGACATAAATGTGGTTGCTGTGATCTACAACTTTTg   <  2:672534/151‑1 (MQ=255)                                                                                                                                            tGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGACATTATTCAGTGTGAAATTGACATAAATGTGGTTGCTGTGATCTACAACTTTTGt  >  1:669820/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                              TTCCAGTAAGCGTATTACCAAGCAGTTGTGTGTTTTGAGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGAGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGACATTATTCAGTGTGAAATTGACATAAATGTGGTTGCTGTGATCTACAACTTTTGT  >  NC_000913/149981‑150269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑