Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	798,688	C→T	P35S (CCG→TCG)	pgl →	6‑phosphogluconolactonase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	798,688	0	C	T	100.0%	193.0 / NA	49	P35S (CCG→TCG)	pgl	6‑phosphogluconolactonase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/23);  total (26/23)

ATTTTAGCTGTTACAGTCAGTTGCTAAATGCAAAGGAGCATTCATGAAGCAAACAGTTTATATCGCCAGCCCTGAGAGCCAGCAAATTCACGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGCCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGTCTGCGCTGCCGG  >  NC_000913/798543‑798829                                                                                                                                                  |                                                                                                                                              aTTTTAGCTGTTACAGTCAGTTGCTAAATGCAAAGGAGCATTCATGAAGCAAACAGTTTATATCGCCAGCCCTGAGAGCCAGCAAATTCACGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGTCgggg                                                                                                                                          >  2:472019/1‑151 (MQ=255)  ttttAGCTGTTACAGTCAGTTGCTAAATGCAAAGGAGCATTCATGAAGCAAACAGTTTATATCGCCAGCCCTGAGAGCCAGCAAATTCACGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGTCGGGGc                                                                                                                                         >  2:824775/1‑151 (MQ=255)        cTGTTACAGTCAGTTGCTAAATGCAAAGGAGCATTCATGAAGCAAACAGTTTATATCGCCAGCCCTGAGAGCCAGCAAATTCACGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGTCGGGGCAgggg                                                                                                                                    >  2:34737/1‑148 (MQ=255)        cTGTTACAGTCAGTTGCTAAATGCAAAGGAGCATTCATGAAGCAAACAGTTTATATCGCCAGCCCTGAGAGCCAGCAAATTCACGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGTCGGGGCAgg                                                                                                                                      >  2:777192/1‑148 (MQ=255)        cTGTTACAGTCAGTTGCTAAATGCAAAGGAGCATTCATGAAGCAAACAGTTTATATCGCCAGCCCTGAGAGCCAGCAAATTCACGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGTCGGGGCAGGt                                                                                                                                     >  2:777210/1‑149 (MQ=255)            tACAGTCAGTTGCTAAATGCAAAGGAGCATTCATGAAGCAAACAGTTTATATCGCCAGCCCTGAGAGCCAGCAAATTCACGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGTCGGGGCAGGTGCAGcc                                                                                                                               >  1:619307/1‑151 (MQ=255)            tACAGTCAGTTGCTAAATGCAAAGGAGCATTCATGAAGCAAACAGTTTATATCGCCAGCCCTGAGAGCCAGCAAATTCACGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGTCGGGGCAGGTGCAGc                                                                                                                                <  2:383920/150‑1 (MQ=255)              cagtcagtTGCTAAATGCAAAGGAGCATTCATGAAGCAAACAGTTTATATCGCCAGCCCTGAGAGCCAGCAAATTCACGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGTCGGGGCAGGTGCAGCCg                                                                                                                              >  2:437703/1‑150 (MQ=255)                  cagtTGCTAAATGCAAAGGAGCATTCATGAAGCAAACAGTTTATATCGCCAGCCCTGAGAGCCAGCAAATTCACGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGTCGGGGCAGGTGCAGCCGAtggt                                                                                                                         <  2:478512/151‑1 (MQ=255)                          aaaTGCAAAGGAGCATTCATGAAGCAAACAGTTTATATCGCCAGCCCTGAGAGCCAGCAAATTCACGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGTCGGGGCAGGTGCAGCCGATGGTGGTCAGcc                                                                                                                 >  2:370038/1‑151 (MQ=255)                            aTGCAAAGGAGCATTCATGAAGCAAACAGTTTATATCGCCAGCCCTGAGAGCCAGCAAATTCACGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGTCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCg                                                                                                               <  1:830160/151‑1 (MQ=255)                              gCAAAGGAGCATTCATGAAGCAAACAGTTTATATCGCCAGCCCTGAGAGCCAGCAAATTCACGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGTCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGa                                                                                                             <  2:454417/151‑1 (MQ=255)                                aaaGGAGCATTCATGAAGCAAACAGTTTATATCGCCAGCCCTGAGAGCCAGCAAATTCACGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGTCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACa                                                                                                           <  2:907052/151‑1 (MQ=255)                                    gAGCATTCATGAAGCAAACAGTTTATATCGCCAGCCCTGAGAGCCAGCAAATTCACGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGTCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACg                                                                                                       >  1:215364/1‑151 (MQ=255)                                      gCATTCATGAAGCAAACAGTTTATATCGCCAGCCCTGAGAGCCAGCAAATTCACGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGTCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGtt                                                                                                     <  2:231648/151‑1 (MQ=255)                                            aTGAAGCAAACAGTTTATATCGCCAGCCCTGAGAGCCAGCAAATTCACGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGTCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTAtctc                                                                                                <  2:897124/150‑1 (MQ=255)                                            aTGAAGCAAACAGTTTATATCGCCAGCCCTGAGAGCCAGCAAATTCACGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGTCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTAtctc                                                                                                >  1:897197/1‑150 (MQ=255)                                              gAAGCAAACAGTTTATATCGCCAGCCCTGAGAGCCAGCAAATTCACGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGTCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTAt                                                                                             >  2:110185/1‑151 (MQ=255)                                               aaGCAAACAGTTTATATCGCCAGCCCTGAGAGCCAGCAAATTCACGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGTCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATg                                                                                            <  2:819871/151‑1 (MQ=255)                                                  cAAACAGTTTATATCGCCAGCCCTGAGAGCCAGCAAATTCACGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGTCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGtt                                                                                          >  2:890000/1‑150 (MQ=255)                                                  cAAACAGTTTATATCGCCAGCCCTGAGAGCCAGCAAATTCACGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGTCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTg                                                                                         >  2:384793/1‑151 (MQ=255)                                                  cAAACAGTTTATATCGCCAGCCCTGAGAGCCAGCAAATTCACGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGTCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTg                                                                                         <  1:184335/151‑1 (MQ=255)                                                       aGTTTATATCGCCAGCCCTGAGAGCCAGCAAATTCACGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGTCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGtt                                                                                    >  1:186969/1‑151 (MQ=255)                                                         tttATATCGCCAGCCCTGAGAGCCAGCAAATTCACGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGTCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGtt                                                                                    >  2:743644/1‑149 (MQ=255)                                                          ttATATCGCCAGCCCTGAGAGCCAGCAAATTCACGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGTCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGc                                                                                 >  1:583921/1‑151 (MQ=255)                                                          ttATATCGCCAGCCCTGAGAGCCAGCAAATTCACGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGTCGGGGCAGGTGCAGCCGATGGGGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCg                                                                                  >  2:70124/1‑150 (MQ=255)                                                            atatCGCCAGCCCTGAGAGCCAGCAAATTCACGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGTCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGGGTTCGccc                                                                               >  1:537988/1‑151 (MQ=255)                                                                     gCCCTGAGAGCCAGCAAATTCACGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGTCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGGCAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTc                                                                       >  2:213184/1‑150 (MQ=255)                                                                     gCCCTGAGAGCCAGCAAATTCACGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGTCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTcg                                                                      <  1:196363/151‑1 (MQ=255)                                                                      cccTGAGAGCCAGCAAATTCACGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGTCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCTGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTcg                                                                      <  2:435204/150‑1 (MQ=255)                                                                            gagCCAGCAAATTCACGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGTCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTg                                                               <  1:597622/151‑1 (MQ=255)                                                                             agCCAGCAAATTCACGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGTCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTgg                                                              <  2:885039/151‑1 (MQ=255)                                                                                 aGCAAATTCACGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGTCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGcg                                                            >  2:565596/1‑149 (MQ=255)                                                                                   cAAATTCACGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGTCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGcgtatc                                                        <  2:900718/151‑1 (MQ=255)                                                                                      aTTCACGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGTCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGcgtatcgta                                                     <  2:284938/151‑1 (MQ=255)                                                                                          aCGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGTCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGc                                                 >  1:43510/1‑151 (MQ=255)                                                                                                 gAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGTCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGCTGGTGGTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCggtc                                          >  1:189674/1‑149 (MQ=255)                                                                                                      tGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGTCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATgg                                     >  1:262994/1‑151 (MQ=255)                                                                                                       gAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGTCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGgc                                    >  1:187184/1‑151 (MQ=255)                                                                                                       gAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGTCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGgc                                    >  1:749356/1‑151 (MQ=255)                                                                                                            aTGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGTCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACt                               <  1:451791/151‑1 (MQ=255)                                                                                                                  gcgcACTGACGCTGACACAGGTTGTCGATGTGTCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCtt                         <  2:651779/151‑1 (MQ=255)                                                                                                                         gACGCTGACACAGGTTGTCGATGTGTCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCa                  <  2:730021/151‑1 (MQ=255)                                                                                                                           cGCTGACACAGGTTGTCGATGTGTCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGc                   >  2:356947/1‑148 (MQ=255)                                                                                                                           cGCTGACACAGGTTGTCGATGTGTCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCaga                <  1:389477/151‑1 (MQ=255)                                                                                                                               gACACAGGTTGTCGATGTGTCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGTCt            <  1:565646/151‑1 (MQ=255)                                                                                                                               gACACAGGTTGTCGATGTGTCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGTCt            <  1:110207/151‑1 (MQ=255)                                                                                                                                 cacaGGTTGTCGATGTGTCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGTCTgc          <  1:332134/151‑1 (MQ=255)                                                                                                                                         gTCGATGTGTCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGTCTGCGCTGCCgg  <  1:700418/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                              ATTTTAGCTGTTACAGTCAGTTGCTAAATGCAAAGGAGCATTCATGAAGCAAACAGTTTATATCGCCAGCCCTGAGAGCCAGCAAATTCACGTCTGGAATCTGAATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGCCGGGGCAGGTGCAGCCGATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGTCTGCGCTGCCGG  >  NC_000913/798543‑798829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑