Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	811,250	A→G	A290A (GCA→GCG)	bioF →	8‑amino‑7‑oxononanoate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	811,250	0	A	G	100.0%	144.9 / NA	43	A290A (GCA→GCG)	bioF	8‑amino‑7‑oxononanoate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/24);  total (19/24)

GCGGGGCAGCGGTGCTTTGCTCCAGTACGGTGGCGGATTATCTGCTGCAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCACGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTGATAACAGCCGTGCGTTACAACTGGCAGAAA  >  NC_000913/811102‑811389                                                                                                                                                     |                                                                                                                                            gCGGGGCAGCGGTGCTTTGCTCCAGTACGGTGGCGGATTATCTGCTGCAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGcgcg                                                                                                                                           >  2:781460/1‑151 (MQ=255)  cGGGGCAGCGGTGCTTTGCTCCAGTACGGTGGCGGATTATCTGCTGCAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCgg                                                                                                                                          <  1:753785/151‑1 (MQ=255)  cGGGGCAGCGGTGCTTTGCTCCAGTACGGTGGCGGATTATCTGCTGCAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCgg                                                                                                                                          >  1:571678/1‑151 (MQ=255)     ggCAGCGGTGCTTTGCTCCAGTACGGTGGCGGATTATCTGCTGCAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCgcggcg                                                                                                                                        >  2:719160/1‑150 (MQ=255)         gCGGTGCTTTGCTCCAGTACGGTGGCGGATTATCTGCTGCAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGaaa                                                                                                                                   <  1:222221/151‑1 (MQ=255)          cGGTGCTTTGCTCCAGTACGGTGGCGGATTATCTGCTGCAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGaaaa                                                                                                                                  <  1:120892/151‑1 (MQ=255)           ggTGCTTTGCTCCAGTACGGTGGCGGATTATCTGCTGCAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGaaaaa                                                                                                                                 <  2:691881/151‑1 (MQ=255)                  tGCTCCAGTACGGTGGCGGATTATCTGCTGCAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTggcgg                                                                                                                          >  2:379672/1‑151 (MQ=255)                   gCTCCAGTACGGTGGCGGATTATCTGCTGCAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTggcgg                                                                                                                          <  2:257538/150‑1 (MQ=255)                              gTGGCGGATTATCTGCTGCAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTAcgc                                                                                                              <  1:388650/151‑1 (MQ=255)                                ggCGGATTATCTGCTGCAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTAcgcg                                                                                                             <  2:589794/150‑1 (MQ=255)                                        aTCTGCTGCAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTg                                                                                                     <  1:456136/150‑1 (MQ=255)                                         tCTGCTGCAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGcc                                                                                                   >  1:273436/1‑151 (MQ=255)                                          ctgctgCAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCg                                                                                                  >  1:282820/1‑151 (MQ=255)                                            gctgcAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGa                                                                                                <  1:426171/151‑1 (MQ=255)                                                 aaTTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTAc                                                                                            >  1:845866/1‑150 (MQ=255)                                                        ccgccACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTg                                                                                    <  2:715035/151‑1 (MQ=255)                                                          gccACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCCTTCCCAGTGATGTGGGTGATGCGCGGCGAGAAAAACTGGCGGCACTCATTACGCGTTTTGGTGGCGGCGTACAGGATTTGcc                                                                                  >  2:663159/1‑151 (MQ=255)                                                          gccACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGcc                                                                                  <  1:342098/151‑1 (MQ=255)                                                          gccACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGcc                                                                                  >  1:780382/1‑151 (MQ=255)                                                            cACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGt                                                                                <  2:807471/151‑1 (MQ=255)                                                                    cTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCtt                                                                        >  2:265393/1‑151 (MQ=255)                                                                      aCAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGGGCCGGAGTACAGGATTTGCCGTTTACGCTTGc                                                                      >  1:406954/1‑151 (MQ=255)                                                                          cACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGAt                                                                  <  1:164488/151‑1 (MQ=255)                                                                          cACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGGGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGAt                                                                  >  2:861099/1‑151 (MQ=255)                                                                            ccAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTc                                                                <  2:15799/151‑1 (MQ=255)                                                                            ccAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGGGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTc                                                                >  1:385704/1‑151 (MQ=255)                                                                             cAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTc                                                                >  1:418041/1‑150 (MQ=255)                                                                              aGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCAt                                                              >  2:794757/1‑151 (MQ=255)                                                                                             cAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAg                                                <  1:460954/150‑1 (MQ=255)                                                                                                 cgcAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCatt                                           >  1:400968/1‑151 (MQ=255)                                                                                                 cgcAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCatt                                           <  2:659237/151‑1 (MQ=255)                                                                                                  gcAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCattg                                          <  1:638003/151‑1 (MQ=255)                                                                                                   cAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCattg                                          >  2:231350/1‑150 (MQ=255)                                                                                                          tACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCgg                                  >  1:443184/1‑151 (MQ=255)                                                                                                               gCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTGAt                              <  1:555465/150‑1 (MQ=255)                                                                                                                cGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTGATaa                            <  1:608338/151‑1 (MQ=255)                                                                                                                   cGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTGATAACa                          <  2:500240/150‑1 (MQ=255)                                                                                                                        gCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTGATAACAGCCGTg                    >  2:585047/1‑151 (MQ=255)                                                                                                                         cGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTGATAACAGCCGTGc                   <  2:622140/151‑1 (MQ=255)                                                                                                                          ggTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTGATAACAGCCGTGCg                  <  1:340747/151‑1 (MQ=255)                                                                                                                                 cGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTGATAACAGCCGTGCGTTACaa            <  2:744203/150‑1 (MQ=255)                                                                                                                                     gTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTGATAACAGCCGTGCGTTACAACTGGc       >  1:466561/1‑151 (MQ=255)                                                                                                                                       gatgaGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTGATAACAGCCGTGCGTTACAACTGGCAg     <  2:423342/151‑1 (MQ=255)                                                                                                                                           aGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTGATAACAGCCGTGCGTTACAACTGGCAGaaa  >  1:141327/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                            GCGGGGCAGCGGTGCTTTGCTCCAGTACGGTGGCGGATTATCTGCTGCAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCACGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTGATAACAGCCGTGCGTTACAACTGGCAGAAA  >  NC_000913/811102‑811389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑