Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	832,662	T→C	M97V (ATG→GTG)	ybiA ←	DUF1768 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	832,662	0	T	C	100.0%	191.8 / NA	55	M97V (ATG→GTG)	ybiA	DUF1768 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/28);  total (27/28)

CGATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCATCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAAT  >  NC_000913/832520‑832802                                                                                                                                               |                                                                                                                                             cGATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCCGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGtt                                                                                                                                      >  2:378127/1‑151 (MQ=255)  gATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTc                                                                                                                                     >  1:59303/1‑151 (MQ=255)   aTTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCt                                                                                                                                    <  1:6825/151‑1 (MQ=255)      cTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCtt                                                                                                                                   <  1:429372/149‑1 (MQ=255)      cTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTg                                                                                                                                 >  1:403247/1‑151 (MQ=255)        tGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCttt                                                                                                                                  <  1:202926/148‑1 (MQ=255)        tGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTg                                                                                                                                 <  1:547456/149‑1 (MQ=255)        tGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGa                                                                                                                                <  1:140412/150‑1 (MQ=255)                ccATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTccc                                                                                                                       >  1:399365/1‑151 (MQ=255)                         ccGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCATCCAGTTTTGCGGGCCCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGAGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTa                                                                                                               <  2:811330/150‑1 (MQ=255)                          cGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCTGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACg                                                                                                             <  2:289858/151‑1 (MQ=255)                           gTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGc                                                                                                            <  1:6921/151‑1 (MQ=255)                               ccccAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCag                                                                                                          >  1:71116/1‑149 (MQ=255)                                     tAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCtt                                                                                                    >  1:705677/1‑149 (MQ=255)                                     tAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCtt                                                                                                    >  1:223670/1‑149 (MQ=255)                                           tCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGAt                                                                                            >  1:564591/1‑151 (MQ=255)                                             gTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATcg                                                                                          >  1:580685/1‑151 (MQ=255)                                                 tCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGcc                                                                                      >  1:521696/1‑151 (MQ=255)                                                          tCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCAtgcgtg                                                                             >  1:503979/1‑151 (MQ=255)                                                          tCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCACAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCAtgcgtg                                                                             >  1:71463/1‑151 (MQ=255)                                                            aaCCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCAtgcgtgcg                                                                           <  1:803627/151‑1 (MQ=255)                                                                aGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGa                                                                       <  2:602486/151‑1 (MQ=255)                                                                   tttGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGAcc                                                                     <  1:245876/150‑1 (MQ=255)                                                                   tttGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGAcc                                                                     >  2:529427/1‑150 (MQ=255)                                                                      gCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATa                                                                  <  1:355537/150‑1 (MQ=255)                                                                       cGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAgg                                                                >  2:189027/1‑151 (MQ=255)                                                                        gggCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAggg                                                               >  2:26235/1‑151 (MQ=255)                                                                         ggCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAgggg                                                              <  1:509687/151‑1 (MQ=255)                                                                              ggTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGTCCCATGCGTGCGGCGACCATAGGGgaaga                                                          >  2:179143/1‑150 (MQ=255)                                                                               gTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGaaa                                                        <  1:243462/151‑1 (MQ=255)                                                                                  gCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAAccc                                                     >  1:653976/1‑151 (MQ=255)                                                                                  gCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAAccc                                                     <  1:515872/151‑1 (MQ=255)                                                                                    cAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCg                                                    >  1:606196/1‑150 (MQ=255)                                                                                     aGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGAc                                                  <  1:446586/151‑1 (MQ=255)                                                                                     aGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGAc                                                  <  1:489720/151‑1 (MQ=255)                                                                                       cAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACgg                                                <  2:739413/151‑1 (MQ=255)                                                                                        aaGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACgg                                                >  2:92190/1‑150 (MQ=255)                                                                                            gcgcACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGAtctc                                           <  2:426236/151‑1 (MQ=255)                                                                                              gcacgcaGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCtt                                         >  1:363059/1‑151 (MQ=255)                                                                                               cacgcaGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTc                                        <  2:511350/151‑1 (MQ=255)                                                                                                acgcaGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGa                                               <  2:814015/143‑1 (MQ=255)                                                                                                acgcaGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGa                                               >  1:814080/1‑143 (MQ=255)                                                                                                            aTGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTtc                            >  2:275900/1‑150 (MQ=255)                                                                                                                    cGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGa                    <  2:567228/150‑1 (MQ=255)                                                                                                                    cGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGa                    <  2:328056/150‑1 (MQ=255)                                                                                                                    cGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGa                    <  2:109682/150‑1 (MQ=255)                                                                                                                     gAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAAt                  >  2:85928/1‑151 (MQ=255)                                                                                                                       aTTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAAttt                <  1:315524/151‑1 (MQ=255)                                                                                                                           aGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTtg             <  1:478813/150‑1 (MQ=255)                                                                                                                            gcgcGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTtgtg           <  2:236400/151‑1 (MQ=255)                                                                                                                               cgAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCt        >  1:702563/1‑151 (MQ=255)                                                                                                                                  aGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGAATTTTTCATCAAGGAATTTTTGTGCCTg       >  2:859888/1‑149 (MQ=255)                                                                                                                                    tGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGaaa    >  1:355868/1‑150 (MQ=255)                                                                                                                                     gCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAAt  <  2:625516/151‑1 (MQ=255)                                                                                                                                      cTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAAt  >  2:433750/1‑150 (MQ=255)                                                                                                                                               |                                                                                                                                             CGATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCATCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAAT  >  NC_000913/832520‑832802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑