Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	846,072	T→C	H131R (CAT→CGT)	glnQ ←	glutamine transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	846,072	0	T	C	100.0%	195.4 / NA	55	H131R (CAT→CGT)	glnQ	glutamine transporter subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/33);  total (22/33)

ATAACCTTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAAC  >  NC_000913/845928‑846218                                                                                                                                                 |                                                                                                                                                   aTAACCTTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCAc                                                                                                                                              >  2:471593/1‑151 (MQ=255) aTAACCTTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCAc                                                                                                                                              <  2:135448/151‑1 (MQ=255)   aaCCTTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACg                                                                                                                                             >  2:86472/1‑150 (MQ=255)   aaCCTTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGt                                                                                                                                            >  1:49243/1‑151 (MQ=255)       ttCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTg                                                                                                                                        >  2:314160/1‑151 (MQ=255)         cAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGAAGTGACGTGGACGCTCTg                                                                                                                                        >  2:454301/1‑149 (MQ=255)          aGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCa                                                                                                                                     <  2:256289/151‑1 (MQ=255)           gCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAg                                                                                                                                    <  1:314186/151‑1 (MQ=255)               ttCATGGCGCAGTTCAGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCg                                                                                                                                >  1:243749/1‑151 (MQ=255)                    ggCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACttt                                                                                                                           <  2:375781/151‑1 (MQ=255)                             ccGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCcagcag                                                                                                                  <  1:609846/151‑1 (MQ=255)                               ggttCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCTCCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCt                                                                                                                <  1:493454/148‑1 (MQ=255)                                     agagCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTg                                                                                                          <  1:575706/151‑1 (MQ=255)                                     agagCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTg                                                                                                          >  1:513512/1‑151 (MQ=255)                                        gCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCa                                                                                                       <  1:107958/151‑1 (MQ=255)                                        gCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCa                                                                                                       <  1:688230/151‑1 (MQ=255)                                           gAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGtt                                                                                                    <  1:797621/151‑1 (MQ=255)                                           gAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGtt                                                                                                    >  2:463567/1‑151 (MQ=255)                                               tCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGtttt                                                                                                  <  1:683262/149‑1 (MQ=255)                                                    tcatcaAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGcc                                                                                            <  1:332565/150‑1 (MQ=255)                                                         aaaCAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCtcttct                                                                                      <  1:625845/151‑1 (MQ=255)                                                            cagcacgatatgcGGCTTTACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTTCCAGACCGACTTTCGCCAGCATCTCACGTGCCAGTTTTTCCGCCTCTTCttt                                                                                    <  2:873855/138‑1 (MQ=255)                                                            cAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCttt                                                                                    >  2:464288/1‑150 (MQ=255)                                                             aGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGt                                                                                  >  1:620078/1‑151 (MQ=255)                                                             aGCATCATTTTCGGCTCCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTg                                                                                   <  2:847515/150‑1 (MQ=255)                                                                  catTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGAGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTcgc                                                                              <  1:302878/150‑1 (MQ=255)                                                                      ttCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCcac                                                                         >  2:624746/1‑151 (MQ=255)                                                                        cGGCTTCTCCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCcac                                                                         <  2:1519/149‑1 (MQ=255)                                                                          gCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCcacgcac                                                                     <  2:19621/151‑1 (MQ=255)                                                                             tCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCacgcacgc                                                                   <  2:669543/150‑1 (MQ=255)                                                                              cACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGt                                                                 >  1:689567/1‑151 (MQ=255)                                                                               aCCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTa                                                                <  1:566360/151‑1 (MQ=255)                                                                                  gCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCg                                                             >  1:543479/1‑151 (MQ=255)                                                                                   ccAGCGCGCGGGCAATCGCCACCCGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCgg                                                            <  1:383541/151‑1 (MQ=255)                                                                                    cAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCggg                                                           >  2:536559/1‑151 (MQ=255)                                                                                    cAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCggg                                                           <  2:411097/151‑1 (MQ=255)                                                                                                actCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCGCGTTCTGCCAGACCGACTTTCGCCAGCAGCTCGCGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACg                                               <  2:191573/149‑1 (MQ=255)                                                                                                aaTCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACg                                               >  1:509410/1‑151 (MQ=255)                                                                                                   cGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGtt                                             <  2:663033/150‑1 (MQ=255)                                                                                                        cacGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCa                                        <  2:775489/150‑1 (MQ=255)                                                                                                         acGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAgc                                      >  2:612556/1‑151 (MQ=255)                                                                                                          cGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAgcg                                     <  1:54804/151‑1 (MQ=255)                                                                                                           gctgctGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTcg                                                                               >  1:478611/1‑108 (MQ=255)                                                                                                           gctgctGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTcg                                                                               <  2:478564/108‑1 (MQ=255)                                                                                                           gctgctGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAgcgc                                    >  2:514521/1‑151 (MQ=255)                                                                                                              gctgTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCt                                   <  1:107350/149‑1 (MQ=255)                                                                                                              gctgTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGt                                 <  1:821614/151‑1 (MQ=255)                                                                                                               ctgTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTc                                >  2:686220/1‑151 (MQ=255)                                                                                                                    gACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATg                           >  1:897907/1‑151 (MQ=255)                                                                                                                         ccaGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCggg                       <  2:154252/150‑1 (MQ=255)                                                                                                                             aaaGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAag                    >  2:365425/1‑149 (MQ=255)                                                                                                                                   cGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTaaaa            <  1:409604/151‑1 (MQ=255)                                                                                                                                    ggAAGGGTAGTGGCGTTCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGTCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGGACGCGTAGCGGTCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAAc           <  2:748959/151‑1 (MQ=255)                                                                                                                                    ggAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAAc           <  1:902745/151‑1 (MQ=255)                                                                                                                                             gTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAac  >  1:238008/1‑151 (MQ=255)                                                                                                                                                 |                                                                                                                                                   ATAACCTTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAAC  >  NC_000913/845928‑846218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑