Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	904,502	T→C	intergenic (‑35/‑91)	ybjP ← / → ybjQ	lipoprotein/UPF0145 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	904,502	0	T	C	100.0%	214.5 / NA	59	intergenic (‑35/‑91)	ybjP/ybjQ	lipoprotein/UPF0145 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/28);  total (31/28)

TCAACGCAAGGACCACTGCGTGTGCCGTTATCTTTATAAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGTTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGAATAT  >  NC_000913/904355‑904643                                                                                                                                                    |                                                                                                                                              tCAACGCAAGGACCACTGCGTGTGCCGTTATCTTTATAAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTc                                                                                                                                             <  1:161296/150‑1 (MQ=255)  cAACGCAAGGACCACTGCGTGTGCCGTTATCTTTATAAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTa                                                                                                                                           <  1:546282/151‑1 (MQ=255)      gCAAGGACCACTGCGTGTGCCGTTATCTTTATAAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGat                                                                                                                                       <  1:28293/151‑1 (MQ=255)      gCAAGGACCACTGCGTGTGCCGTTATCTTTATAAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGat                                                                                                                                       <  1:20725/151‑1 (MQ=255)       cAAGGACCACTGCGTGTGCCGTTATCTTTATAAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGata                                                                                                                                      <  1:124671/151‑1 (MQ=255)       cAAGGACCACTGCGTGTGCCGTTATCTTTATAAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGata                                                                                                                                      <  1:278167/151‑1 (MQ=255)        aaGGACCACTGCGTGTGCCGTTATCTTTATAAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGata                                                                                                                                      >  2:46800/1‑150 (MQ=255)         aGGACCACTGCGTGTGCCGTTATCTTTATAAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGataat                                                                                                                                    <  1:654377/151‑1 (MQ=255)          ggACCACTGCGTGTGCCGTTATCTTTATAAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGataata                                                                                                                                   <  2:885623/151‑1 (MQ=255)          ggACCACTGCGTGTGCCGTTATCTTTATAAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGataata                                                                                                                                   <  2:787737/151‑1 (MQ=255)              cACTGCGTGTGCCGTTATCTTTATAAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATg                                                                                                                                >  1:860686/1‑150 (MQ=255)               aCTGCGTGTGCCGTTATCTTTATAAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGat                                                                                                                              >  2:366557/1‑151 (MQ=255)                  gCGTGTGCCGTTATCTTTATAAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATc                                                                                                                           <  1:269055/151‑1 (MQ=255)                  gCGTGTGCCGTTATCTTTATAAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATc                                                                                                                           <  2:316954/151‑1 (MQ=255)                       tgCCGTTATCTTTATAAGCTGGTGTGACTGTGGTGCAGTCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTc                                                                                                                      >  2:381652/1‑151 (MQ=255)                        gCCGTTATCTTTATAAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCa                                                                                                                     <  2:799670/151‑1 (MQ=255)                        gCCGTTATCTTTATAAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCa                                                                                                                     <  2:617529/151‑1 (MQ=255)                             tATCTTTATAAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTa                                                                                                                                           <  1:498768/124‑1 (MQ=255)                             tATCTTTATAAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTa                                                                                                                                           >  2:498719/1‑124 (MQ=255)                             tATCTTTATAAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCaataat                                                                                                                >  1:153397/1‑151 (MQ=255)                             tATCTTTATAAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCaataa                                                                                                                 <  2:198201/150‑1 (MQ=255)                                cTTTATAAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGc                                                                                                             >  2:285473/1‑151 (MQ=255)                                cTTTATAAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGc                                                                                                             >  1:28554/1‑151 (MQ=255)                                   tataAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGt                                                                                                           >  2:673393/1‑150 (MQ=255)                                    ataAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTAt                                                                                                         >  1:719193/1‑151 (MQ=255)                                      aaGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATc                                                                                                        <  2:179798/150‑1 (MQ=255)                                        gCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGt                                                                                                     >  2:367427/1‑151 (MQ=255)                                          tGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCt                                                                                                   >  1:766749/1‑151 (MQ=255)                                           ggAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAg                                                                                                              <  1:278808/139‑1 (MQ=255)                                           ggAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAg                                                                                                              >  2:278782/1‑139 (MQ=255)                                           ggAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTg                                                                                                  >  1:58989/1‑151 (MQ=255)                                              gTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATa                                                                                               <  2:602176/151‑1 (MQ=255)                                                           aGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTg                                                                                      >  2:895142/1‑147 (MQ=255)                                                               aCTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGaa                                                                              <  2:480733/151‑1 (MQ=255)                                                                 tGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAgg                                                                            >  1:17795/1‑151 (MQ=255)                                                                  gaggagCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAgg                                                                            <  1:95404/150‑1 (MQ=255)                                                                        cAGTGCGCAGGGGATAACCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATACTAGCGTATCAGTCTGATAATGCTTTTGACATCGAAGGc                                                                           <  2:18399/145‑1 (MQ=255)                                                                            gcgcAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAAc                                                                  >  2:404241/1‑150 (MQ=255)                                                                             cgcAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACa                                                                 >  2:537359/1‑150 (MQ=255)                                                                              gcAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACa                                                                 >  1:309917/1‑149 (MQ=255)                                                                               cAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATc                                                                                 <  1:579733/132‑1 (MQ=255)                                                                               cAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATc                                                                                 >  2:579683/1‑132 (MQ=255)                                                                                       aaGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAgatcgat                                                      <  2:100615/151‑1 (MQ=255)                                                                                             tGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGc                                                 >  2:223978/1‑150 (MQ=255)                                                                                              gTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCaa                                               <  1:353568/151‑1 (MQ=255)                                                                                                cAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAAtt                                             >  2:895967/1‑151 (MQ=255)                                                                                                  aTTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAAtttt                                           <  2:302850/151‑1 (MQ=255)                                                                                                   tttGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTc                                          >  2:369664/1‑151 (MQ=255)                                                                                                     tGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATTCAATTTTCt                                         <  1:619531/150‑1 (MQ=255)                                                                                                                     gATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATCATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATACTGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTg                          >  1:189450/1‑149 (MQ=255)                                                                                                                          ccTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGcc                   <  1:380997/151‑1 (MQ=255)                                                                                                                          ccTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGcc                   >  2:270330/1‑151 (MQ=255)                                                                                                                              ataAGCGCACGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACACCAACTCTGGAAGGCCAGAc               >  2:74436/1‑151 (MQ=255)                                                                                                                               taAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGAcc              >  1:160521/1‑151 (MQ=255)                                                                                                                                    gATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGt         >  2:898796/1‑151 (MQ=255)                                                                                                                                    gATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGt         >  2:53189/1‑151 (MQ=255)                                                                                                                                       cGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTg       <  2:285683/150‑1 (MQ=255)                                                                                                                                        gCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGa      >  1:877333/1‑150 (MQ=255)                                                                                                                                           ctGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGAatat  >  1:775828/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                              TCAACGCAAGGACCACTGCGTGTGCCGTTATCTTTATAAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGTTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGAATAT  >  NC_000913/904355‑904643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑