Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	973,216	C→T	L62L (CTG→CTA)	elyC ←	envelope biogenesis factor; DUF218 superfamily protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	973,216	0	C	T	100.0%	197.5 / NA	52	L62L (CTG→CTA)	elyC	envelope biogenesis factor; DUF218 superfamily protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/16);  total (36/16)

TCATTCAGGCGTGGCAGACTGTTATTGATTAAATTAGAGCTCGGTGCCCACTGCGGGTTCCAGGTATAACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGCAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCGCCAGGCCAGCGCCAATAATCAACAACATCAGCGGAAGTGGCAGC  >  NC_000913/973070‑973363                                                                                                                                                   |                                                                                                                                                    tcattcaGGCGTGGCAGACTGTTATTGATTAAATTAGAGCTCGGTGCCCACTGCGGGTTCCAGGTATAACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGc                                                                                                                                                 >  1:38868/1‑151 (MQ=255)         gCGTGGCAGACTGTTATTGATTAAATTAGAGCTCGGTGCCCTCTGCGGGTTCCAGGTATAACCCCCTCCGAGCCCCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCt                                                                                                                                         <  2:889567/151‑1 (MQ=255)          cGTGGCAGACTGTTATTGATTAAATTAGAGCTCGGTGCCCACTGCGGGTTCCAGGTATAACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCt                                                                                                                                         >  2:669763/1‑150 (MQ=255)               cAGACTGTTATTGATTAAATTAGAGCTCGGTGCCCACTGCGGGTTCCAGGTATAACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCggc                                                                                                                                   >  2:253666/1‑150 (MQ=255)               cAGACTGTTATTGATTAAATTAGAGCTCGGTGCCCACTGCGGGTTCCAGGTATAACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGt                                                                                                                                   <  2:273863/151‑1 (MQ=255)                  aCTGTTATTGATTAAATTAGAGCTCGGTGCCCACTGCGGGTTCCAGGTATAACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGc                                                                                                                                >  1:280415/1‑151 (MQ=255)                    tGTTATTGATTAAATTAGAGCTCGGTGCCCACTGCGGGTTCCAGGTATAACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCa                                                                                                                               >  2:683958/1‑150 (MQ=255)                                   tAGAGCTCGGTGCCCACTGCGGGTTCCAGGTATAACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAag                                                                                                                 >  2:353803/1‑149 (MQ=255)                                         tCGGTGCCCACTGCGGGTTCCAGGTATAACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCgcc                                                                                                           >  1:564630/1‑149 (MQ=255)                                           ggTGCCCACTGCGGGTTCCAGGTATAACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCgccagcc                                                                                                       <  1:765331/151‑1 (MQ=255)                                                ccACTGCGGGTTCCAGGTATAACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCAccc                                                                                                   >  1:602388/1‑150 (MQ=255)                                                           tCCAGGTATAACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCtaataa                                                                                        <  2:754569/150‑1 (MQ=255)                                                            ccAGGTATAACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATaaa                                                                                       <  1:94291/150‑1 (MQ=255)                                                               ggTATAACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATc                                                                                   >  1:624369/1‑151 (MQ=255)                                                                gTATAACCACCTCCGAGCACCACGATATAGTCCTCTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCt                                                                                  <  1:359977/151‑1 (MQ=255)                                                                 tataACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCtt                                                                                 >  2:836814/1‑151 (MQ=255)                                                                 tataACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCtt                                                                                 >  1:230654/1‑151 (MQ=255)                                                                        accTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGtt                                                                          >  1:709609/1‑151 (MQ=255)                                                                         ccTCCGAGCACCACGATATAGTCCGCTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGtt                                                                          <  2:492087/150‑1 (MQ=255)                                                                         ccTCCGAGCACCACGATATAGTACACTTTTTGCGAATTATTCCATGTCGGATAGGCGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGttt                                                                         >  2:422292/1‑151 (MQ=255)                                                                            ccGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGtttt                                                                        <  2:549084/149‑1 (MQ=255)                                                                            ccGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGtttt                                                                        <  1:507028/149‑1 (MQ=255)                                                                                   ccacGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACg                                                               >  1:22717/1‑151 (MQ=255)                                                                                      cGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCt                                                            <  1:845139/151‑1 (MQ=255)                                                                                          ataGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAAc                                                        >  1:242063/1‑151 (MQ=255)                                                                                           taGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAAcc                                                       >  2:9086/1‑151 (MQ=255)                                                                                               ccACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACcaca                                                    <  2:47193/150‑1 (MQ=255)                                                                                                 aCTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCAcagc                                                  >  1:352223/1‑150 (MQ=255)                                                                                                  cTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCAcagc                                                  >  1:48072/1‑149 (MQ=255)                                                                                                   tttttGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACagcagc                                               >  1:285812/1‑151 (MQ=255)                                                                                                    ttttGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAgcg                                              >  1:704534/1‑151 (MQ=255)                                                                                                     tttGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAgcgc                                             >  2:422223/1‑151 (MQ=255)                                                                                                      ttGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAgcgc                                             <  2:328584/150‑1 (MQ=255)                                                                                                      ttGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCgcc                                            >  1:421989/1‑151 (MQ=255)                                                                                                         cGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCgccagg                                         >  2:76870/1‑151 (MQ=255)                                                                                                                ttCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCGCCAGGCCAgcgc                                  >  1:507537/1‑151 (MQ=255)                                                                                                                 tCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCGCCAGGCCAGCGcc                                 >  2:872104/1‑151 (MQ=255)                                                                                                                  ccATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCGCCAGGCCAGCGcc                                 >  2:699462/1‑150 (MQ=255)                                                                                                                   cATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCGCCAGGCCAGCGCCa                                >  2:462762/1‑150 (MQ=255)                                                                                                                    aTGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCGCCAGGCCAGCGCCaat                              >  2:489740/1‑151 (MQ=255)                                                                                                                     tGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCGCCAGGCCAGCGCCaat                              >  1:158240/1‑150 (MQ=255)                                                                                                                     tGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCGCCAGGCCAGCGCCaat                              >  1:391111/1‑150 (MQ=255)                                                                                                                         ggATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCGCCAGGCCAGCGCCaata                             >  1:315647/1‑147 (MQ=255)                                                                                                                              ggTGCTTTCGATGGGACGCAGTAGGTGGTCGGCTACCGGTTACAAACTCAATAACAAGAGCGACAGCCACCCAATGCTAATAAAAACCTTCCCGGTTTTTTGAAAACGGCTAAACCTCAGCAGAGCCAGGCCAGCGCCAATAATcaacaa                     <  2:869615/150‑1 (MQ=255)                                                                                                                              ggTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCGCCAGGCCAGCGCCAATAATCaacaac                    <  2:322367/151‑1 (MQ=255)                                                                                                                               gTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCGCCAGGCCAGCGCCAATAATCaacaac                    >  1:717884/1‑150 (MQ=255)                                                                                                                               gTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCGCCAGGCCAGCGCCAATAATCaacaac                    >  1:22028/1‑150 (MQ=255)                                                                                                                                 gCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCGCCTGGCCAGCGCCAATAATCAACAACATc                 <  2:517323/151‑1 (MQ=255)                                                                                                                                   tttCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCGCCAGGCCAGCGCCAATAATCAACAACATc                 >  1:430163/1‑149 (MQ=255)                                                                                                                                           ggACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCGCCAGGCCAGCGCCAATAATCAACAACATCAGCGGAAGTg       <  1:86733/151‑1 (MQ=255)                                                                                                                                              cGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCGCCAGGCCAGCGCCAATAATCAACAACATCAGCGGAAGTGgc     >  2:630709/1‑150 (MQ=255)                                                                                                                                              cGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCGCCAGGCCAGCGCCAATAATCAACAACATCAGCGGAAGTGgc     >  2:274057/1‑150 (MQ=255)                                                                                                                                               gcagtaGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCGCCAGGCCAGCGCCAATAATCAACAACATCAGCGGAAGTGgcag   <  1:738612/151‑1 (MQ=255)                                                                                                                                                cagtagGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCGCCAGGCCAGCGCCAATAATCAACAACATCAGCGGAAGTGgcagc  <  1:353829/151‑1 (MQ=255)                                                                                                                                                cagtagGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCGCCAGGCCAGCGCCAATAATCAACAACATCAGCGGAAGTGgcag   >  1:637327/1‑150 (MQ=255)                                                                                                                                                   |                                                                                                                                                    TCATTCAGGCGTGGCAGACTGTTATTGATTAAATTAGAGCTCGGTGCCCACTGCGGGTTCCAGGTATAACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGCAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCGCCAGGCCAGCGCCAATAATCAACAACATCAGCGGAAGTGGCAGC  >  NC_000913/973070‑973363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑