Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,022,227	A→G	Y553H (TAT→CAT)	yccS ←	putative transporter, FUSC superfamily inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,022,227	0	A	G	100.0%	202.3 / NA	57	Y553H (TAT→CAT)	yccS	putative transporter, FUSC superfamily inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/31);  total (26/31)

ATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTAACGCTACTTCAAAACCT  >  NC_000913/1022079‑1022372                                                                                                                                                     |                                                                                                                                                  aTTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGcc                                                                                                                                                 <  2:764566/151‑1 (MQ=255)    tGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGATATGCCGa                                                                                                                                               <  2:757254/150‑1 (MQ=255)     gCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACa                                                                                                                                             <  2:132645/151‑1 (MQ=255)       ggggTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAg                                                                                                                                            >  2:500934/1‑150 (MQ=255)       ggggTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGt                                                                                                                                           >  1:905792/1‑151 (MQ=255)         ggTACCGTTCCGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTg                                                                                                                                         <  2:707119/151‑1 (MQ=255)           tAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGc                                                                                                                                       >  2:444106/1‑151 (MQ=255)               gTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCctct                                                                                                                                   >  1:273067/1‑151 (MQ=255)                   ggCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTgg                                                                                                                               >  2:327645/1‑151 (MQ=255)                           ggACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGtt                                                                                                                         <  1:103081/149‑1 (MQ=255)                              cATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGc                                                                                                                    <  1:585979/151‑1 (MQ=255)                               atatTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCa                                                                                                                   <  2:524778/151‑1 (MQ=255)                                     gATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATgcgc                                                                                                              <  2:142662/150‑1 (MQ=255)                                      aTACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCgg                                                                                                            <  1:480758/151‑1 (MQ=255)                                         ccaccGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCa                                                                                                          <  2:716137/150‑1 (MQ=255)                                         ccaccGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAg                                                                                                         <  1:463330/151‑1 (MQ=255)                                           accGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGc                                                                                                           >  1:901607/1‑147 (MQ=255)                                           accGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGAt                                                                                                       <  2:1281/151‑1 (MQ=255)                                             cGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTg                                                                                                     <  2:392933/151‑1 (MQ=255)                                                 gCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGa                                                                                                  >  2:612984/1‑150 (MQ=255)                                                    aGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACt                                                                                              <  1:24501/151‑1 (MQ=255)                                                     gCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTg                                                                                             <  1:720137/151‑1 (MQ=255)                                                       tCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGcc                                                                                           >  1:752103/1‑151 (MQ=255)                                                        cAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGc                                                                                            <  2:299392/149‑1 (MQ=255)                                                         aGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAg                                                                                         <  2:815468/151‑1 (MQ=255)                                                            aTCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCa                                                                                      <  2:315273/151‑1 (MQ=255)                                                             tCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCa                                                                                      <  2:678715/150‑1 (MQ=255)                                                              cACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAgg                                                                                    >  2:252201/1‑151 (MQ=255)                                                              cACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAg                                                                                     <  2:710767/150‑1 (MQ=255)                                                                cGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGcc                                                                                  >  2:873625/1‑151 (MQ=255)                                                                         gCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGc                                                                         <  2:574610/151‑1 (MQ=255)                                                                                   gggCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTgccgc                                                               >  2:312186/1‑151 (MQ=255)                                                                                   gggCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTgccgc                                                               >  2:128884/1‑151 (MQ=255)                                                                                   gggCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTgccgc                                                               <  1:461978/151‑1 (MQ=255)                                                                                                cgCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGc                                                   >  2:689391/1‑150 (MQ=255)                                                                                                   cAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGcaca                                                >  1:518582/1‑150 (MQ=255)                                                                                                              cACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGt                                    >  1:61556/1‑151 (MQ=255)                                                                                                                 gCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATc                                 <  2:528121/151‑1 (MQ=255)                                                                                                                  ccccTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCg                                <  2:215480/151‑1 (MQ=255)                                                                                                                   cccTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCg                                >  1:5787/1‑150 (MQ=255)                                                                                                                    ccTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGAt                              <  2:41872/151‑1 (MQ=255)                                                                                                                       gATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTAc                           >  2:324581/1‑151 (MQ=255)                                                                                                                       gATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTAc                           <  2:600713/151‑1 (MQ=255)                                                                                                                       gATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTAc                           <  1:485986/151‑1 (MQ=255)                                                                                                                        aTGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTAcg                          >  1:37970/1‑151 (MQ=255)                                                                                                                          ggTACTGCTCCAGTATGGCACCGAGATGCCGACAGTTGGCCTCTGTGGCCCGTTCGAGCAGGCGCGGCAGATTGCGAAACTGCCAGTCACGCCAGATGTAGCCCACTGCCGCCCACGCAATGGCACAACCAAACCGCGTATCGATTAcgc                         >  2:407580/1‑150 (MQ=255)                                                                                                                           gTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTAcgcg                        >  2:32343/1‑150 (MQ=255)                                                                                                                               tGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGt                    >  1:658292/1‑150 (MQ=255)                                                                                                                                gCTCCAGTATGGCATCGAGATGCCGGCAGTTGGCCTCTGTGGCGCGTTCGAGCATGCCCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACGGCCGCCCACGCAACGGCACAACCACTCAGCGTATCGATTACGCGAggga                   >  2:855876/1‑148 (MQ=255)                                                                                                                                   ccAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTAACg                <  1:508437/150‑1 (MQ=255)                                                                                                                                    cAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTAACGCt              >  2:463801/1‑151 (MQ=255)                                                                                                                                      gTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTAACGCTAc            <  1:873107/151‑1 (MQ=255)                                                                                                                                        aTGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTAACGCTACtt          <  2:690118/151‑1 (MQ=255)                                                                                                                                          ggCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTAACGCTACTTCa        <  2:736900/151‑1 (MQ=255)                                                                                                                                             aTCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTAACGCTACTTCaaa      >  2:157540/1‑150 (MQ=255)                                                                                                                                             aTCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTAACGCTACTTCaaa      >  2:598198/1‑150 (MQ=255)                                                                                                                                                gagaTGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTAACGCTACTTCAAAACCt  >  2:332080/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                  ATTTGCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTAACGCTACTTCAAAACCT  >  NC_000913/1022079‑1022372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑