Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,119,351	C→T	G33R (GGA→AGA)	yceO ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,119,351	0	C	T	100.0%	213.9 / NA	56	G33R (GGA→AGA)	yceO	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/31);  total (25/31)

GAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTC  >  NC_000913/1119203‑1119498                                                                                                                                                     |                                                                                                                                                    gagaTGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATctcc                                                                                                                                                   <  2:347356/151‑1 (MQ=255)                   tGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGc                                                                                                                                  >  2:399129/1‑150 (MQ=255)                           cAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGc                                                                                                                         >  2:899999/1‑151 (MQ=255)                               aataatCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTcgc                                                                                                                     <  2:44710/151‑1 (MQ=255)                                ataatCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTcgcg                                                                                                                    <  2:158241/151‑1 (MQ=255)                                       cATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAAtatt                                                                                                              >  2:710266/1‑150 (MQ=255)                                        aTTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAAtattat                                                                                                            >  1:375736/1‑151 (MQ=255)                                          tCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATattatt                                                                                                           >  1:508086/1‑150 (MQ=255)                                          tCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATattatt                                                                                                           <  1:493091/150‑1 (MQ=255)                                           cAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATtga                                                                                                         <  2:595416/151‑1 (MQ=255)                                           cAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATtg                                                                                                          <  2:695848/150‑1 (MQ=255)                                            aaTTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATtgat                                                                                                        <  1:804514/151‑1 (MQ=255)                                             aTTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATtgatg                                                                                                       <  2:802468/151‑1 (MQ=255)                                                  tCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGAt                                                                                                   <  1:450671/150‑1 (MQ=255)                                                   cACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATa                                                                                                  <  1:363069/150‑1 (MQ=255)                                                      gCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGc                                                                                              <  2:643900/151‑1 (MQ=255)                                                          aaTGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACa                                                                                          <  1:296058/151‑1 (MQ=255)                                                             gAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATtga                                                                                                         >  2:859495/1‑133 (MQ=255)                                                             gAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATtga                                                                                                         <  1:859562/133‑1 (MQ=255)                                                                aGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGAc                                                                                    <  1:53998/151‑1 (MQ=255)                                                                 gggAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACg                                                                                   <  2:650309/151‑1 (MQ=255)                                                                 gggAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACg                                                                                   <  1:362736/151‑1 (MQ=255)                                                                     gaTTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCAtt                                                                               >  1:293923/1‑151 (MQ=255)                                                                        tAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTaa                                                                             >  1:72680/1‑150 (MQ=255)                                                                             atTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTc                                                                       <  1:628896/151‑1 (MQ=255)                                                                                tGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCt                                                                     <  2:134497/150‑1 (MQ=255)                                                                                 gTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGt                                                                   <  2:742388/151‑1 (MQ=255)                                                                                  tCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGTGATAGTGCAACAGCCGACGCATTAAATACTCCTGt                                                                   >  1:670213/1‑150 (MQ=255)                                                                                   cAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTa                                                                  <  1:753660/150‑1 (MQ=255)                                                                                    aGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTaaa                                                                <  2:330795/151‑1 (MQ=255)                                                                                      tttATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAAAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTaaaa                                                               >  2:104605/1‑150 (MQ=255)                                                                                         aTGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACg                                                            <  1:324721/150‑1 (MQ=255)                                                                                            tCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGc                                                          >  2:607723/1‑149 (MQ=255)                                                                                            tCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCt                                                        >  1:740834/1‑151 (MQ=255)                                                                                            tCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCt                                                        >  2:449056/1‑151 (MQ=255)                                                                                             cAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTc                                                       <  2:293897/151‑1 (MQ=255)                                                                                                  cGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCt                                                  >  2:555215/1‑151 (MQ=255)                                                                                                  cGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCt                                                  <  1:200024/151‑1 (MQ=255)                                                                                                      aaaCTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAg                                              <  1:882474/151‑1 (MQ=255)                                                                                                         cTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGcc                                           >  2:101695/1‑151 (MQ=255)                                                                                                             aaaTAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTa                                        >  1:190728/1‑150 (MQ=255)                                                                                                               aTAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAatg                                     <  1:34849/151‑1 (MQ=255)                                                                                                                tAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTaa                                       >  1:480674/1‑148 (MQ=255)                                                                                                                   aaCACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAAtgatga                                 <  1:280054/151‑1 (MQ=255)                                                                                                                         tAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTg                           >  1:677884/1‑151 (MQ=255)                                                                                                                         tAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTg                           >  2:657117/1‑151 (MQ=255)                                                                                                                         tAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTg                           >  1:329979/1‑151 (MQ=255)                                                                                                                          agatcagatcCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTgg                          <  2:824853/151‑1 (MQ=255)                                                                                                                              cagatcCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGc                      <  2:40391/151‑1 (MQ=255)                                                                                                                                   ccATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGaa                 <  1:304568/151‑1 (MQ=255)                                                                                                                                     aTAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGaa                 >  2:685027/1‑149 (MQ=255)                                                                                                                                        aTTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCt            >  2:906584/1‑151 (MQ=255)                                                                                                                                        aTTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCt            <  1:738321/151‑1 (MQ=255)                                                                                                                                          tGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTg           >  1:376040/1‑150 (MQ=255)                                                                                                                                             caacaaTCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATg       <  2:117244/151‑1 (MQ=255)                                                                                                                                                 aaTCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGt   >  1:247681/1‑151 (MQ=255)                                                                                                                                                  aTCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGtc  >  2:547226/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                    GAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTC  >  NC_000913/1119203‑1119498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑