Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,213,552	A→G	W170R (TGG→CGG)	bluR ←	repressor of blue light‑responsive genes

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,213,552	0	A	G	100.0%	207.7 / NA	56	W170R (TGG→CGG)	bluR	repressor of blue light‑responsive genes
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/21);  total (35/21)

CCTGTCCAGACAATCAATGTTTGCGTCGGGAACAGTTCCGGGCGCGGCGATTCAATGGGATCAGCAAGAACAGAAATGTGCCATCCTGACAATGATAAACGCCATGCTTCCAGCCACAATCGTGCTCTCTCTTCAACATTCCACGCCATCAGAATGGCTTCTTTACCGGGCTTACGGCGCATTTCGAAAAGCGAGGTTGCTGCGTATTCAATTAATGCGCCGTCAAACATACTGCTCATAATGCGGGAGGTGTTGTGATCAAGCACGAGACGCTGGCGAACAGGAAGG  >  NC_000913/1213410‑1213697                                                                                                                                               |                                                                                                                                                  ccTGTCCAGACAATCAATGTTTGCGTCGGGAACAGTTCCGGGCGCGGCGATTCAATGGGATCAGCAAGAACAGAAATGTGCCATCCTGACAATGATAAACGCCATGCTTCCAGCCACAATCGTGCTCTCTCTTCAACATTCCGCGCCATc                                                                                                                                            >  2:411637/1‑150 (MQ=255)  cggTCCAGACAATCAATGTTTGCGTCGGGAACAGTTCCGGGCGCGGCGATTCAATGGGATCAGCAAGAACAGAAATGTGCCATCCTGACAATGATAAACGCCATGCTTCCAGCCACAATCGTGCTCTCTCTTCAACATTCCGCGCCATCAg                                                                                                                                          <  2:402521/149‑1 (MQ=255)          aCAATCAATGTTTGCGTCGGGAACAGTTCCGGGCGCGGCGATTCAATGGGATCAGCAAGAACAGAAATGTGCCATCCTGACAATGATAAACGCCATGCTTCCAGCCACAATCGTGCTCTCTCTTCAACATTCCGCGCCATCAGAATGGctt                                                                                                                                  <  2:294982/151‑1 (MQ=255)               caatGTTTGCGTCGGGAACAGTTCCGGGCGCGGCGATTCAATGGGATCAGCAAGAACAGAAATGTGCCATCCTGACAATGATAAACGCCATGCTTCCAGCCACAATCGTGCTCTCTCTTCAACATTCCGCGCCATCAGAATGGCTTCttt                                                                                                                              <  1:586509/150‑1 (MQ=255)                    tttGCGTCGGGAACAGTTCCGGGCGCGGCGATTCAATGGGATCAGCAAGAACAGAAATGTGCCATCCTGACAATGATAAACGCCATGCTTCCAGCCACAATCGTGCTCTCTCTTCAACATTCCGCGCCATCAGAATGGCTTCTTTACCggg                                                                                                                        >  1:123788/1‑151 (MQ=255)                           cGGGAACAGTTCCGGGCGCGGCGATTCAATGGGATCAGCAAGAACAGAAATGTGCCATCCTGACAATGATAAACGCCATGCTTCCAGCCACAATCGTGCTCTCTCTTCAACATTCCGCGCCATCAGAATGGCTTCTTTACCGGGCTTACgg                                                                                                                 >  1:472542/1‑151 (MQ=255)                           cGGGAACAGTTCCGGGCGCGGCGATTCAATGGGATCAGCAAGAACAGAAATGTGCCATCCTGACAATGATAAACGCCATGCTTCCAGCCACAATCGTGCTCTCTCTTCAACATTCCGCGCCATCAGAATGGCTTCTTTACCGGGCTTACgg                                                                                                                 >  1:214465/1‑151 (MQ=255)                             ggAACAGTTCCGGGCGCGGCGATTCAATGGGATCAGCAAGAACAGAAATGTGCCATCCTGACAATGATAAACGCCATGCTTCCAGCCACAATCGTGCTCTCTCTTCAACATTCCGCGCCATCAGAATGGCTTCTTTACCGGGCTTACGgcg                                                                                                               <  2:497785/151‑1 (MQ=255)                               aaCAGTTCCGGGCGCGGCGATTCAATGGGATCAGCAAGAACAGAAATGGGCCATCCTGACAATGATAAACGCCATGCTTCCAGCCACAATCGTGCTCTCTCTTCAACATTCCGCGCCATCAGAATGGCTTCTTTACCGGGCTTACGgcgc                                                                                                              <  2:710848/150‑1 (MQ=255)                                        gggCGCGGCGATTCAATGGGATCAGCAAGAACAGAAATGTGCCATCCTGACAATGATAAACGCCATGCTTCCAGCCACAATCGTGCTCTCTCTTCAACATTCCGCGCCATCAGAATGGCTTCTTTACCGGGCTTACGGCGCATTTCGaaaa                                                                                                    <  2:687108/151‑1 (MQ=255)                                            gcggcgATTCAATGGGATCAGCAAGAACAGAAATGTGCCATCCTGACAATGATAAACGCCATGCTTCCAGCCACAATCGTGCTCTCTCTTCAACATTCCGCGCCATCAGAATGGCTTCTTTACCGGGCTTACGGCGCATTTCGAAAAGCg                                                                                                 >  1:494078/1‑150 (MQ=255)                                             cggcgATTCAATGGGATCAGCAAGAACAGAAATGTGCCATCCTGACAATGATAAACGCCATGCTTCCAGCCACAATCGTGCTCTCTCTTCAACATTCCGCGCCATCAGAATGGCTTCTTTACCGGGCTTACGGCGCATTTCGAAAAGCg                                                                                                 >  1:125608/1‑149 (MQ=255)                                              ggcgATTCAATGGGATCAGCAAGAACAGAAATGTGCCATCCTGACAATGATAAACGCCATGCTTCCAGCCACAATCGTGCTCTCTCTTCAACATTCCGCGCCATCAGAATGGCTTCTTTACCGGGCTTACGGCGCATTTCGAAAAGCGAgg                                                                                              >  1:626489/1‑151 (MQ=255)                                               gcgATTCAATGGGATCAGCAAGAACAGAAATGTGCCATCCTGACAATGATAAACGCCATGCTTCCAGCCACAATCGTGCTCTCTCTTCAACATTCCGCGCCATCAGAATGGCTTCTTTACCGGGCTTACGGCGCATTTCGAAAAGCGAGGt                                                                                             >  1:11293/1‑151 (MQ=255)                                                 gATTCAATGGGATCAGCAAGAACAGAAATGTGCCATCCTGACAATGATAAACGCCATGCTTCCAGCCACAATCGTGCTCTCTCTTCAACATTCCGCGCCATCAGAATGGCTTCTTTACCGGGCTTACGGCGCATTTCGAAAAGCGAGGTtg                                                                                           >  2:147468/1‑151 (MQ=255)                                                  aTTCAATGGGATCAGCAAGAACAGAAATGTGCCATCCTGACAATGATAAACGCCATGCTTCCAGCCACAATCGTGCTCTCTCTTCAACATTCCGCGCCATCAGAATGACTTCTTTACCGGGCTTACGGCGCATTTCGAAAAGCGAGGTtgc                                                                                          >  1:58324/1‑151 (MQ=255)                                                   ttCAATGGGATCAGCAAGAACAGAAATGTGCCATCCTGACAATGATAAACGCCATGCTTCCAGCCACAATCGTGCTCTCTCTTCAACATTCCGCGCCATCAGAATGGCTTCTTTACCGGGCTTACGGCGCATTTCGAAAAGCGAGGTtgct                                                                                         >  2:743781/1‑151 (MQ=255)                                                    tCAATGGGATCAGCAAGAACAGAAATGTGCCATCCTGACAATGATAAACGCCATGCTTCCAGCCACAATCGTGCTCTCTCTTCAACATTCCGCGCCATCAGAATGGCTTCTTTACCGGGCTTACGGCGCATTTCGAAAAGCGAGGTtgctg                                                                                        >  2:874640/1‑151 (MQ=255)                                                             tCAGCAAGAACAGAAATGTGCCATCCTGACAATGATAAACGCCATGCTTCCAGCCACAATCGTGCTCTCTCTTCAACATTCCGCGCCATCAGAATGGCTTCTTTACCGGGCTTACGGCGCATTTCGAAAAGCGAGGTTGCTGCGTATTCa                                                                                >  2:331011/1‑150 (MQ=255)                                                              cAGCAAGAACAGAAATGTGCCATCCTGACAATGATAAACGCCATGCTTCCAGCCACAATCGTGCTCTCTCTTCAACATTCCGCGCCATCAGAATGGCTTCTTTACCGGGCTTACGGCGCATTTCGAAAAGCGAGGTTGCTGCGTATTCaa                                                                               <  1:373748/150‑1 (MQ=255)                                                               aGCAAGAACAGAAATGTGCCATCCTGACAATGATAAACGCCATGCTTCCAGCCACAATCGTGCTCTCTCTTCAACATTCCGCGCCATCAGAATGGCTTCTTTACCGGGCTTACGGCGCATTTCGAAAAGCGAGGTTGCTGCGTATTCAAtt                                                                             >  2:254137/1‑151 (MQ=255)                                                               aGCAAGAACAGAAATGTGCCATCCTGACAATGATAAACGCCATGCTTCCAGCCACAATCGTGCTCCCTCTTCAACATTCCGCGCCATCAGAATGGCTTCTTTACCGGGCTTACGGCGCATTTCGAAAAGCGAGGTTGCTGCGTATTCAAt                                                                              <  1:599736/150‑1 (MQ=255)                                                                gCAAGAACAGAAATGTGCCATTCTGACAATGATAAACGCCATGCTTCCAGCCACAATCGTGCTCTCTCTTCAACATTCCGCGCCATCAGAATGGCTTCTTTACCGGGCTTACGGCGCATTTCGAAAAGCGAGGTTGCTGCGTATTCAACTa                                                                            <  1:103150/151‑1 (MQ=255)                                                                gCAAGAACAGAAATGTGCCATCCTGACAATGATAAACGCCATGCTTCCAGCCACAATCGTGCTCTCTCTTCAACATTCCGCGCCATCAGAATGGCTTCTTTACCGGGCTTACGGCGCATTTCGAAAAGCGAGGTTGCTGCGTATTCAATTa                                                                            <  1:103135/151‑1 (MQ=255)                                                                  aaGAACAGAAATGTGCCATCCTGACAATGATAAACGCCATGCTTCCAGCCACAATCGTGCTCTCTCTTCAACATTCCGCGCCATCAGAATGGCTTCTTTACCGGGCTTACGGCGCATTTCGAAAAGCGAGGTTGCTGCGTATTCAATTAAt                                                                          >  2:24366/1‑151 (MQ=255)                                                                  aaGAACAGAAATGTGCCATCCTGACAATGATAAACGCCATGCTTCCAGCCACAATCGTGCTCTCTCTTCAACATTCCGCGCCATCAGAATGGCTTCTTTACCGGGCTTACGGCGCATTTCGAAAAGCGAGGTTGCTGCGTATTCAATTAAt                                                                          <  1:295504/151‑1 (MQ=255)                                                                     aaCAGAAATGTGCCATCCTGACAATGATAAACGCCATGCTTCCAGCCACAATCGTGCTCTCTCTTCAACATTCCGCGCCATCAGAATGGCTTCTTTACCGGGCTTACGGCGCATTTCGAAAAGCGAGGTTGCTGCGTATTCAATTAATgcg                                                                       >  2:597044/1‑151 (MQ=255)                                                                      aCAGAAATGTGCCATCCTGACAATGATAAACGCCATGCTTCCAGCCACAATCGTGCTCTCTCTTCAACATTCCGCGCCATCAGAATGGCTTCTTTACCGGGCTTACGGCGCATTTCGAAAAGCGAGGTTGCTGCGTATTCAATTAATgcgc                                                                      >  1:412708/1‑151 (MQ=255)                                                                        aGAAATGTGCCATCCTGACAATGATAAACGCCATGCTTCCAGCCACAATCGTGCTCTCTCTTCAACATTCCGCGCCATCAGAATGGCTTCTTTACCGGGCTTACGGCGCATTTCGAAAAGCGAGGTTGCTGCGTATTCAATTAATGCGCCg                                                                    >  2:17522/1‑151 (MQ=255)                                                                           aaTGTGCCATCCTGACAATGATAAACGCCATGCTTCCAGCCACAATCGTGCTCTCTCTTCAACATTCCGCGCCATCAGAATGGCTTCTTTACCGGGCTTACGGCGCATTTCGAAAAGCGAGGTTGCTGCGTATTCAATTAATGCGCCGTc                                                                  >  1:100990/1‑150 (MQ=255)                                                                            aTGTGCCATCCTGACAATGATAAACGCCATGCTTCCAGCCACAATCGTGCTCTCTCTTCAACATTCCGCGCCATCAGAATGGCTTCTTTACCGGGCTTACGGCGCATTTCGAAAAGCGAGGTTGCTGCGTATTCAATTAATGCGCCGTCaa                                                                <  2:889136/151‑1 (MQ=255)                                                                             tgtgCCATCCTGACAATGATAAACGCCATGCTTCCAGCCACAATCGTGCTCTCTCTTCAACATTCCGCGCCATCAGAATGGCTTCTTTACCGGGCTTACGGCGCATTTCGAAAAGCGAGGTTGCTGCGTATTCAATTAATGCGCCGTCaa                                                                >  1:895357/1‑150 (MQ=255)                                                                                  cATCCTGACAATGATAAACGCCATGCTTCCAGCCACAATCGTGCTCTCTCTTCAACATTCCGCGCCATCAGAATGGCTTCTTTACCGGGCTTACGGCGCATTTCGAAAAGCGAGGTTGCTGCGTATTCAATTAATGCGCCGTCAAACATAc                                                          >  2:94489/1‑151 (MQ=255)                                                                                         aCAATGATAAACGCCATGCTTCCAGCCACAATCGTGCTCTCTCTTCAACATTCCGCGCCATCAGAATGGCTTCTTTACCGGGCTTACGGCGCATTTCGAAAAGCGAGGTTGCTGCGTATTCAATTAATGCGCCGTCAAACATACTGCTCAt                                                   >  2:324797/1‑151 (MQ=255)                                                                                              gATAAACGCCATGCTTCCAGCCACAATCGTGCTCTCTCTTCAACATTCCGCGCCATCAGAATGGCTTCTTTACCGGGCTTACGGCGCATTTCGAAAAGCGAGGTTGCTGCGTATTCAATTAATGCGCCGTCAAACATACTGCTCATAATg                                               <  1:12339/150‑1 (MQ=255)                                                                                              gATAAACGCCATGCTTCCAGCCACAATCGTGCTCTCTCTTCAACATTCCGCGCCATCAGAATGGCTTCTTTACCGGGCTTACGGCGCATTTCGAAAAGCGAGGTTGCTGCGTATTCAATTAATGCGCCGTCAAACATACTGCTCATAATGc                                              >  1:729996/1‑151 (MQ=255)                                                                                                    cGCCATGCTTCCAGCCACAATCGTGCTCTCTCTTCAACATTCCGCGCCATCAGAATGGCTTCTTTACCGGGCTTACGGCGCATTTCGAAAAGCGAGGTTGCTGCGTATTCAATTAATGCGCCGTCAAACATACTGCTCATAATGCGGGAgg                                        >  2:870538/1‑151 (MQ=255)                                                                                                      ccATGCTTCCAGCCACAATCGTGCTCTCTCTTCAACATTCCGCGCCATCAGAATGGCTTCTTTACCGGGCTTACGGCGCATTTCGAAAAGCGAGGTTGCTGCGTATTCAATTAATGCGCCGTCAAACATACTGCTCATAATGCGGGAGgtg                                      >  1:289914/1‑151 (MQ=255)                                                                                                       cATGCTTCCAGCCACAATCGTGCTCTCTCTTCAACATTCCGCGCCATCAGAATGGCTTCTTTACCGGGCTTACGGCGCATTTCGAAAAGCGAGGTTGCTGCGTATTCAATTAATGCGCCGTCAAACATACTGCTCATAATGCGGGAGgtgt                                     >  1:182393/1‑151 (MQ=255)                                                                                                        aTGCTTCCAGCCACAATCGTGCTCTCTCTTCAACATTCCGCGCCATCAGAATGGCTTCTTTACCGGGCTTACGGCGCATTTCGAAAAGCGAGGTTGCTGCGTATTCAATTAATGCGCCGTCAAACATACTGCTCATAATGCGGGAGGtgtt                                    >  1:108282/1‑151 (MQ=255)                                                                                                          gCTTCCAGCCACAATCGTGCTCTCTCTTCAACATTCCGCGCCATCAGAATGGCTTCTTTACCGGGCTTACGGCGCATTTCGAAAAGCGAGGTTGCTGCGTATTCAATTAATGCGCCGTCAAACATACTGCTCATAATGCGGGAGGtgttgt                                  <  2:97501/151‑1 (MQ=255)                                                                                                           cTTCCAGCCACAATCGTGCTCTCTCTTCAACATTCCGCGCCATCAGAATGGCTTCTTTACCGGGCTTACGGCGCATTTCGAAAAGCGAGGTTGCTGCGTATTCAATTAATGCGCCGTCAAACATACTGCTCATAATGCGGGAGGTGTtgtg                                 >  2:143956/1‑151 (MQ=255)                                                                                                            ttCCAGCCACAATCGTGCTCTCTCTTCAACATTCCGCGCCATCAGAATGGCTTCTTTACCGGGCTTACGGCGCATTTCGAAAAGCGAGGTTGCTGCGTATTCAATTAATGCGCCGTCAAACATACTGCTCATAATGCGGGAGGTGTtgtg                                 >  1:408793/1‑150 (MQ=255)                                                                                                              ccAGCCACAATCGTGCTCTCTCTTCAACATTCCGCGCCATCAGAATGGCTTCTTTACCGGGCTTACGGCGCATTTCGAAAAGCGAGGTTGCTGCGTATTCAATTAATGCGCCGTCAAACATACTGCTCATAATGCGGGAGGTGTTGTGAt                               >  2:103325/1‑150 (MQ=255)                                                                                                                 gCCACAATCGTGCTCTCTCTTCAACATTCCGCGCCATCAGAATGACTTCTTTACCGGGCTTACGGCGCATTTCGAAAAGCGAGGTTGCTGCGTATTCAATTAATGCGCCGTCAAACATACTGCTCATAATGCGGGAGGTGTTGTGATCAAg                           <  2:58302/151‑1 (MQ=255)                                                                                                                   cacaATCGTGCTCTCTCTTCAACATTCCGCGCCATCAGAATGGCTTCTTTACCGGGCTTACGGCGCATTTCGAAAAGCGAGGTTGCTGCGTATTCAATTAATGCGCCGTCAAACATACTGCTCATAATGCGGGAGGTGTTGTGATCAAGc                          >  1:362338/1‑150 (MQ=255)                                                                                                                      aaTCGTGCTCTCTCTTCAACATTCCGCGCCATCAGAATGGCTTCTTTACCGGGCTTACGGCGCATTTCGAAAAGCGAGGTTGCTGCGTATTCAATTAATGCGCCGTCAAACATACTGCTCATAATGCGGGAGGTGTTGTGATCAAGCACg                       >  2:432015/1‑150 (MQ=255)                                                                                                                       aTCGTGCTCTCTCTTCAACATTCCGCGCCATCAGAATGGCTTCTTTACCGGGCTTACGGCGCATTTCGAAAAGCGAGGTTGCTGCGTATTCAATTAATGCGCCGTCAAACATACTGCTCATAATGCGGGAGGTGTTGTGATCAAGCACgag                     <  2:282512/151‑1 (MQ=255)                                                                                                                         cGTGCTCTCTCTTCAACATTCCGCGCCATCAGAATGGCTTCTTTACCGGGCTTACGGCGCATTTCGAAAAGCGAGGTTGCTGCGTATTCAATTAATGCGCTGTCAAACATACTGCTCATAATGCGGGAGGTGTTGTGATCAAGCACGAGAc                   <  2:14866/151‑1 (MQ=255)                                                                                                                         cGTGCTCTCTCTTCAACATTCCGCGCCATCAGAATGGCTTCTTTACCGGGCTTACGGCGCATTTCGAAAAGCGAGGTTGCTGCGTATTCAATTAATGCGCCGTCAAACATACTGCTCATAATGCGGGAGGTGTTGTGATCAAGCACgaga                    <  2:243349/150‑1 (MQ=255)                                                                                                                            gCTCTCTCTTCAACATTCCGCGCCATCAGAATGGCTTCTTTACCGGGCTTACGGCGCATTTCGAAAAGCGAGGTTGCTGCGTATTCAATTAATGCGCCGTCAAACATACTGCTCATAATGCGGGAGGTGTTGTGATCAAGCACGAGACGCt                >  2:283669/1‑151 (MQ=255)                                                                                                                               ctctctTCAACATTCCGCGCCATCAGAATGGCTTCTTTACCGGGCTTACGGCGCATTTCGAAAAGCGAGGTTGCTGCGTATTCAATTAATGCGCCGTCAAACATACTGCTCATAATGCGGGAGGTGTTGTGATCAAGCACGAGACGCTg               <  1:142329/149‑1 (MQ=255)                                                                                                                                tctctTCAACATTCCGCGCCATCAGAATGGCTTCTTTACCGGGCTTACGGCGCATTTCGAAAAGCGAGGTTGCTGCGTATTCAATTAATGCGCCGTCAAACATACTGCTCATAATGCGGGAGGTGTTGTGATCAAGCACGAGACGCTGGCg            >  2:715983/1‑151 (MQ=255)                                                                                                                                      cAACATTCCGCGCCATCAGAATGGCTTCTTTACCGGGCTTACGGCGCATTTCGAAAAGCGAGGTTGCTGCGTATTCAATTAATGCGCCGTCAAACATACTGCTCATAATGCGGGAGGTGTTGTGATCAAGCACGAGACGCTGGCGAACAg       <  2:472495/150‑1 (MQ=255)                                                                                                                                         cATTCCGCGCCATCAGAATGGCTTCTTTACCGGGCTTACGGCGCATTTCGAAAAGCGAGGTTGCTGCGTATTCAATTAATGCGCCGTCAAACATACTGCTCATAATGCGTGAGGTGTTGTGATCAAGCACGAGACGCTGGCGAACAGGAAg   <  2:185389/151‑1 (MQ=255)                                                                                                                                          aTTCCGCGCCATCAGAATGGCTTCTTTACCGGGCTTACGGCGCATTTCGAAAAGCGAGGTTGCTGCGTATTCAATTAATGCGCCGTCAAACATACTGCTCATAATGCGGGAGGTGTTGTGATCAAGCACGAGACGCTGGCGAACAGGAAgg  >  1:285078/1‑151 (MQ=255)                                                                                                                                               |                                                                                                                                                  CCTGTCCAGACAATCAATGTTTGCGTCGGGAACAGTTCCGGGCGCGGCGATTCAATGGGATCAGCAAGAACAGAAATGTGCCATCCTGACAATGATAAACGCCATGCTTCCAGCCACAATCGTGCTCTCTCTTCAACATTCCACGCCATCAGAATGGCTTCTTTACCGGGCTTACGGCGCATTTCGAAAAGCGAGGTTGCTGCGTATTCAATTAATGCGCCGTCAAACATACTGCTCATAATGCGGGAGGTGTTGTGATCAAGCACGAGACGCTGGCGAACAGGAAGG  >  NC_000913/1213410‑1213697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑